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Network Ca2+-Cell Activity Field CA3 Hippocampal Slices of Rat Early and Late Postnatal Development
Mitaeva Y.I.1, Mozherov A.M.1, Mukhina I.V.1'2
1 Lobachevsky State University of Nizhni Novgorod, Nizhny Novgorod, Russian Federation;
2 Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation.
Information processing in the brain - is the result of the constant interaction between two cellular networks: the neuronal and the glial. Hippocampus - the structure of the central nervous system, which is involved in the mechanisms of emotion and memory consolidation. The hippocampus has a certain topology distribution of cellular elements, which provides the many cellular networks. One of them is the network of neurons in the CA3 field. This network receives inputs from cells of the entorhinal cortex and the dentate gyrus, in addition CA3 pyramidal neurons form the connection between themselves and interneurons, forming a closed network that operates in conditions of acute slice and generates spontaneous Ca2+ activity. Neuronal network interacts with the glial network, the main manifestation of activity which are Ca2+ oscillations. Therefore, to estimate the age dependence of Ca2+ activity in the cells were investigated Ca2+ oscillations in neuronal and glial networks and the interactions between them.
In this work, we investigated changes in the characteristics of Ca2+ oscillations cells of rat hippocampal CA3 field in early (P5-8, P14-16) and late (P21-25), postnatal development. Also shown the effect of temperature of perfusion solution on cells Ca2+ activity of CA3 field hippocampal slices of rats in different postnatal periods. Besides in the study was valued role of network activity in the formation of spontaneous Ca2+ oscillations cells of rat hippocampal CA3 field in early and late stages of postnatal development. Experiments were carried out on acute hippocampal slices from rats. Was used laser scanning confocal microscope Carl Zeiss LSM 510 Duoscan (Germany). Recording fluorescence kinetics were carried out in full frame (field of view of 400x400 mm), with a resolution of 512x512 pixels digital and scanning frequency of 1 Hz. Fluorescence indicators recorded in the range 500-530 nm (Oregon Green 488 BAPTA-1 AM) and 650-710 nm (Sulforhodamine 101). The fluorescence intensity (s.u.) shows the dependence of the concentration of [Ca2+]i in time, indicating the metabolic activity of cells. Method of cross - correlation analysis was used to evaluate synchrony of Ca2+ oscillations cells of CA3 field of rat hippocampus. We chose the time interval size in 3 seconds and within this interval were found synchronous Ca2+ oscillations in all possible pairs of cells. Further, the number of synchronously occurring Ca2+ oscillations were normalized to the minimum number of Ca2+ oscillations in one of the cells analyzed pairs.
The studies have shown that the parameters of cell Ca2+ oscillations field CA3 of hippocampal slices vary depending on the period of postnatal rats. Reducing the amount of Ca2+ oscillations with age due to the formation and complexity of synaptically connected neural networks, the transition of electrical synapses in the chemical. Transitional period is 14-16 days of postnatal development, and for 21 days - there is a fully formed neural network. Electrically connected network is weakly controlled, excitement is freely distributed over the network, involving work of all cells, resulting in a high Ca2+ activity in rat hippocampal cells of younger age group. In mature hippocampal brain slices spontaneous Ca2+ activity with low due to lack of active neural network. In this case, the spontaneous Ca2+ oscillations are due mainly metabolic activity of cells has been shown in our experiments. This study showed that changes in Ca2+ activity in the cells of rat hippocampal CA3 fields occurring during postnatal development directly related to the functioning of the neural networks, and the metabolic state of the cells. Ca2+ signaling in mature brain - is a complex multicomponent process involving various receptor systems capable of mutual substitution in violation of the normal functioning of one or more of them.
OM&P
8 Opera Med Physiol 2017 Vol. 3 (S1)
