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Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv
original article | UDC 636.03 | doi: 10.32718/ujvas4-3.05
Natural biologically active additive in feeding calves
Volume 4 Number 3
V. F. Radchikov1, V. P. Tzai1, A. N. Kot1, T. L. Sapsaleva1, G. V. Besarab1, B. V. Gutyj2, V. I. Karpovskyi3, V. O. Trokoz3
1Scientific and Practical Center of the National Academy of Sciences Belarus on animal husbandry, Frynze Str., 11, Zhodino, 222160, Belarus
2Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Pekarska Str., 50, Lviv, 79010, Ukraine
3National University of Life and Environmental Sciences of Ukraine, Heroiv Oborony Str., 15, Kyiv, 03041, Ukraine
Article info Received 12.07.2021 Received in revised form
27.08.2021 Accepted 28.08.2021
Correspondence author Vasilij Radchikov Tel.: +37-529-627-20-65 E-mail: arud22222@gmail.com
2021 Radchikov V. et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Studies on the effectiveness of the inclusion of sodium humate in the diet of young cattle were conducted on four groups of animals. Installed improvement in the consumption of hay by the bulls of the experimental groups by 12.5-20 % compared with the control young was found, as a result of which they consumed more feed units by 1.5, 2.3, and 3.5 %, exchange energy - by 2.4, 3.9 and 5.1 %, digestible protein - by 1.2, 2.1 and 3.7 %. As a result of the inclusion of sodium humate in the diet as part of the KR-2 compound feed, the amount of hemoglobin in the blood of animals of the II experimental group increased by 5.8 %, in III - by 6.8, in IV - by 7.8 % compared to control analogs. With the growth of calves in the blood, the bactericidal activity of blood serum increased by 1.3, 1.9, and 2.5 %, lysozyme activity increased by 0.1 %, 0.2, 0.3 %, which indicates an increase in natural resistance in animals whose diets were injected with the studied peat and sapropel preparation. The use of sodium humate in the feed of bulls in the composition of compound feed KR-2 has a positive effect on the feed consumption, physiological condition, resistance of animals, which provides an increase in the average daily increase in live weight by 3.2-9.4 % while reducing the cost of its production by 2.9-8.5 percent.
Keywords: bulls, feed, sodium humate, resistance, productivity, efficiency.
Contents
1. Introduction................. .. 28
2. Materials and methods .... .. 29
3. Results and discussion .... .. 29
4. Conclusions................ .. 31
References ....... .. 31
Citation:
Radchikov, V. F., Tzai, V. P., Kot, A. N., Sapsaleva, T. L., Besarab, G. V., Gutyj, B. V., Karpovskyi, V. I., & Trokoz, V. O. (2021). Natural biologically active additive in feeding calves. Ukrainian Journal of Veterinary and Agricultural Sciences, 4(3), 28-32. \_/
1. Introduction
Feeding of farm animals must be carried out according to detailed standards, taking into account the chemical composition and nutritional value of the feed used. This makes it possible to more fully balance the diets, thereby increasing the productivity of animals at the exact feed costs (Slavetsky, 2002; Razumovsky et al., 2002; Yakovchik & Ganushchenko, 2011; Johansson et al., 2012; Amamou et al., 2019; Borshch et al., 2021; Denkovich et al., 2021; Mylostyvyi et al., 2021).
It is possible to provide animal diets with protein, carbohydrates, mineral, and biologically active substances by feeding various feed additives and premixes (Jeroch, 2008; Bogdanovich & Razumovsky, 2019; Petrushko & Bogda-
novich, 2019; Prilovskaya et al., 2020; Razumovsky et al., 2020; Vlizlo et al., 2021; Bashchenko et al., 2021).
The creation of a new generation of feed additives with functional properties is of great importance in preparing diets. The inclusion of feed additives with prebiotics in the diet allows you to give the product these properties. Such products support physiological health and reduce the risk of diseases (De Oliveira et al., 2011; Acedo et al., 2011; Bogdanovich, 2019; Bogdanovich & Razumovsky, 2019; Radchikova et al., 2019; Bogdanovich & Razumovsky, 2020).
Such additives in animal feeding make it possible to compensate for the lack of energy, plastic, and regulatory nutrients in the body. It also has a regulating effect on physiological functions and biochemical reactions. This makes it possible to maintain physiological health and reduce the risk
of diseases, including those caused by a violation of the microbial biocenosis of the digestive tract of farm animals (Huuskonen et al., 2009; Shareiko et al., 2013; Valero et al., 2014; Tamkovich et al., 2015).
Inexpensive, highly effective biologically active substances of natural origin are in great demand since they are the most accessible, non-toxic, and do not have an undesirable effect on the animal's body during prolonged feeding (Shareiko et al., 2011; Suchkova et al., 2013; Bogdanovich & Razumovsky, 2019).
One of these additives is sodium humate (humiliate), obtained from peat and sapropel. It has been established that the drug contains several macro-and microelements and amino acids that enter into complex bonds with the help of humic acids. However, its widespread use in feeding farm animals is hindered by insufficient knowledge of the drug's
Table 1
Scheme of experience
effect on the physiological state and productivity of animals. The norms of its feeding have not been established, which was the reason for our research.
The purpose of the research is to study the effectiveness of sodium humate in feeding young cattle.
2. Materials and methods
The studies were carried out on four groups of young cattle of a black-and-white breed with an average live weight of 79-81 kg of 12 heads each (Table 1).
The differences in feeding consisted in the fact that the bulls of the experimental groups were additionally fed sodium humate in doses of 0.4 (Il-experimental), 0.5 ml (III-experimental), and 0.6 ml (IV-experimental) per 1 kg of live weight.
Age of Number of_Duration of the period, day
Group animals, months. animals, heads Preparatory-6 Main-54
I - II - control experienced 3 3 12 12 MD MD The main diet (MD) - WMR, compound feed KR-2, clover-hay timofeevka The main diet (MD) +compound feed in a dose of sodium humate 0.4 ml/kg of live weight
III IV - experienced - experienced 3 3 12 12 MD MD The main diet (MD) + compound feed in a dose of sodium humate 0.5 ml/kg of live weight The main diet (MD) + compound feed in a dose of sodium humate in a dose of 0.6 ml/kg of live weight
All the manipulations with the animals were conducted according to the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (Official Journal of the European Union L276/33, 2010).
3. Results and discussion
The use of KR-2 compound feed in bulls feeding with the inclusion of a feed additive sodium humate had a positive effect on the feed consumption (Table 2).
Studies have found an increase in hay consumption by the bulls of the experimental groups by 12.5-20 % compared to the control ones. As a result, the animals of the
Table 2
Rations of experienced animals
experimental groups consumed more feed units by 1.5, 2.3, and 3.5 %, exchange energy - by 2.4, 3.9, and 5.1 %, digestible protein - by 1.2, 2.1, and 3.7 %. The fiber content was 17.8-17.9 % of the dry matter of the diet. The sugar-protein ratio was at the level of - 0.84-0.55:1. The ratio of calcium to phosphorus in all groups was 1.53-1.65:1.
Hematological indicators also indicate an increase in metabolic processes in the body of animals of the experimental groups (Table 3).
As a result of the conducted studies, it was found that after feeding the sodium humate preparation as part of the KR-2 compound feed, the amount of hemoglobin in the II experimental group increased by 5.8 %, in the III - by 6.8, in the IV - by 7.8 % compared with control peers.
Feed and nutrients I Y_l L \J II III IV
Compound feed KR-2, kg 1.5 1.5 1.5 1.5
Clover-timothy hay, kg 0.8 0.9 0.95 0.97
WMR, g 6.0 6.0 6.0 6.0
Milk, l 2.0 2.2 2.3 2.5
The diet contains:
Feed units 3.45 3.50 3.53 3.57
Metabolizable energy, MJ 33.2 34.0 34.3 34,9
Dry matter, kg 3.06 3.15 3.21 3,30
Crude protein, g 467 477 485 490
Digestible protein, g 328 332 335 340
Crude fat, g 164 165 167 170
Crude fiber, g 545 561 575 589
Sugar, g 177 180 183 187
Calcium, g 19.8 20.5 21.0 22.1
Phosphorus, g 12.9 13.1 13.4 13.4
A tendency has been established to increase the total protein in the blood of calves of experimental groups (II, III, and IV) with the introduction of feed additives by 5.6, 8.1, and 10.7 % compared with control analogs.
The use of sodium feed humate additive in feeding young cattle had a positive effect on phosphorus metabolism. The concentration of this trace element increased in the II experimental group by 3.4 %, in the III - by 4.5 %, and in the IV - by 5.5 % compared to the control group (Table 4).
The calcium content in the blood of experimental calves in comparison with the control indicators increased by 6.8 % (II), 7.2 % (III), and 7.7 % (IV) groups.
Table 3
Morpho-biochemical composition of blood
The results of studies on the effect of the sodium humate preparation in the compound feed on the natural resistance of calves are shown in Table 5.
With the growth of calves' blood, the bacterial activity of blood serum increased by 1.3, 1.9, and 2.5 %, lysozyme activity - by 0.1 %, 0.2 %, 0.3 %. Consequently, the natural resistance of animals, whose diet also included sodium humate from peat and sapropel, significantly increased during the experiment.
Studies have shown that the average daily gains of calves in the control group amounted to 898 g (Table 6).
Indicator
Group
III
IV
Red blood cells, 1012/l 6.3 ± 0.21 6.9 ± 0.29 7.0 ± 0.29 7.1 ± 0.29
Hemoglobin, g/l 102 ± 0.27 108 ± 0.25 109 ± 0.25 110 ± 0.25
White blood cells, 109/l 7.59 ± 0.03 7.52 ± 0.03 7.56 ± 0.04 7.58 ± 0.04
Total protein, g/l 71.05 ± 0.29 75.2 ± 0.29 77.3 ± 0.29 79.5 ± 0.29
Glucose, mmol/l 4.0 ± 0.15 4.2 ± 0.08 4.3 ± 0.11 4.4 ± 0.22
Acid capacity, mg% 440 ± 2.47 460 ± 2.04 470 ± 2.08 480 ± 2.16
Urea, mmol/l 4.08 ± 0.87 3.81 ± 0.89 4.11 ± 0.14 4.11 ± 0.15
I
II
Table 4
Blood mineral composition
Indicator
Group
III
IV
Calcium, mmol/l 3.74 ± 0.06 4.01 ± 0.14 4.03 ± 0.03 4.05 ± 0.08
Phosphorus, mmol/l 2.60 ± 0.04 2.69 ± 0.06 2.72 ± 0.10 2.75 ± 0.05
Magnesium, mmol/l 1.23 ± 0.02 1.23 ± 0.02 1.23 ± 0.02 1.25 ± 0.02
Potassium, mmol/l 9.9 ± 0.04 10.0 ± 0.5 10.3 ± 0.4 10.3 ± 0.4
Sodium, mmol/l 110.3 ± 2.7 110.5 ± 3.3 111.0 ± 3.1 111.1 ± 3.2
Iron, mmol/l 18.7 ± 0.89 18.9 ± 0.87 19.1 ± 0.88 20.3 ± 0.86
Zinc, mmol/l 4.6 ± 3.4 4.6 ± 3.8 4.65 ± 4.5 4.7 ± 1.7
Manganese, mmol/l 1.7 ± 0.1 1.73 ± 0.1 1.75 ± 0.1 1.77 ± 0.1
Copper, mmol/l 12.1 ± 0.78 12.3 ± 0.93 12.4 ± 0.79 12.9 ± 0.48
I
II
Table 5
Natural resistance of experimental animals
Indicator
Group
I
II
III
IV
Bactericidal activity of blood serum, % Lysozyme activity, % beta-lysozyme activity of blood serum
62.3 ± 1.2 6.4 ± 0.29 19.2 ± 0.28
63.6 ± 1.0 6.5 ± 0.35 19.3 ± 0.31
64.2 ± 1.3 6.6 ± 0.33 19.4 ± 0.31
64.8 ± 1.4 6.7 ± 0.31 19.5 ± 0.33
Table 6
Live weight and average daily gain of experience animals
Indicator
Group
I
II
III
IV
Bodyweight. g at the beginning of the experience at the end of the experience Gross increase, kg Average daily weight gain, g % to control
Feed costs per 1 kg of grain, k units. % to control
79.0 ± 1.81 132.9 ± 4.04 53.9 ± 4.5 898 ± 10.2 100 3.84 100.0
79.5 ± 2.15 135.1 ± 3.93
55.6 ± 40.10 927 ± 12.3
103.2 3.78 98.4
80.0 ± 8.6 137.4 ± 3.68 57.4 ± 3.90 957 ± 10.8 106.6 3.69 96.1
81.0 ± 1.91 139.9 ± 3.71 58.9 ± 3.95 982 ± 12.9 109.4 3.57 93.0
The use of sodium humate in feeding young cattle at the rate of 0.4 ml, 0.5, and 0.6 ml per 1 kg of live weight provided an average daily increase in live weight at the level of 927; 957 and 982 g (groups II, III, IV) or 3.2 6.6 and 9.4 % higher than in the control group.
Feeding of 0.4 ml of sodium humate per 1 kg of live weight per day to young cattle as part of compound feed led to a decrease in the cost of growth by 2.9 %, with the inclusion of 0.5 ml per 1 kg of live weight - by 6 % at a dose of 0.6 ml per 1 kg of live weight - by 8.5 % relative to control animals.
4. Conclusions
The inclusion of sodium humate in the diet of young cattle in the composition of compound feed KR-2 has a positive effect on feed consumption, physiological condition, the resistance of animals, which provides an increase in the average daily increase in live weight by 3.2-9.4 %, reducing the cost of its production by 2.9-8.5 percent.
Conflict of interest
The authors declare that there is no conflict of interest. References
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