CC BY
28B-P-15
Mutual influence of intense LED light and cold signaling through the CRISPR/Cas9-edited HOS1 gene
V.P. Bulgakov1, G.N. Veremeichik1, T.Y. Gorpenchenko1, Y.A. Yugay1, T.V. Avramenko1, Y.N.
Shkryl1, E.P. Subbotin2, Y.N. Kulchin2
1 Federal Scientific Center of the East Asia Terrestrial Biodiversity (Institute of Biology and Soil Science), Far Eastern Branch of the Russian Academy of Sciences, 159 Stoletija Str., Vladivostok, 690022, Russia 2 Institute of Automation and Control Processes Far Eastern Branch of the Russian Academy of Sciences (IACP FEB RAS), Far Eastern Branch of the Russian Academy of Sciences, 5 Radio str., Vladivostok, 690041, Russia
V.P. Bulgakov: bulgakov@biosoil.ru
E3 ubiquitin-protein ligase HOS1 is a key integrator of temperature information and developmental processes. However, HOS1 connection with the central integrator of the light signaling system, transcription factor HY5 is unknown. HY5 acts downstream of the light receptor network and directly affects transcription of numerous light-induced genes. Here we studied the intersection of intense LED lighting and cold acclimation signaling pathways by using hos1Cas9 Arabidopsis plants, in which the HOS1 gene was subjected to genome editing via the CRISPR/Cas9 technology. The hos1Cas9 plants were highly resistant to cold stress. When hos1Cas9 plants were exposed to cold stress combined with intense LED lighting (HL, 1200 ^mol m-2 s-1), the light signaling system underwent significant changes due to increased expression of the HY5 gene. The hos1 mutation changed the balance between reactive oxygen species (ROS) production and ROS decomposition in cells via modulation of gene activity. Single-cell confocal imaging showed that levels of ROS were decreased in hos1Cas9 mutant plants under combined action of cold and HL. Co-regulation of cold-induced and HL-induced ROS appears to be accomplished by the HOS1 —I ICE1 —I ABI5 ^ RbohD and HOS1 —I PIF4(HY5) ^ ABI5 ^ RbohD signaling modules. These data show that the search for the optimal combination of strong LED illumination and resistance to temperature stress during plant cultivation should be continued by regulating the expression of the HOS1 and HY5 genes.
