CC BY
6*
ALT'23 The 30th International Conference on Advanced Laser Technologies
B-I-34
Multiphoton microscopy of intrinsic fluorophores in biological tissue
Wei Zheng
Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 1068Xueyuan Boulevard, Nanshan, Shenzhen 518055,China [email protected]. cn
Multiphoton microscopy (MPM) allows observation of cellular and subcellular dynamics and functions in deep live tissue within highly complex and heterogeneous environments, providing critical in situ and in vivo information for biological studies. In past decades, we have witnessed the striking development of MPM which has significantly revolutionized biomedical research. In this talk, I will first briefly review the recent advances of MPM and then focus on our new development of MPM techniques. A time- and spectral- resolved detection system was used in our home-made MPM system to provide the capability for detecting fluorescence lifetime and fluorescence spectra simultaneously. Furthermore, a short wavelength femtosecond fiber laser was used to extend the excitation source. To demonstrate the versatility of our novel MPM system, we carried out MPM imaging of endogenous fluorophores in live cells and tissues.
