CC BY
13
BecmHUK Ka^HMy №2-2018
y^K 591.8: 504
Z.B. Yessimsiitova1, A.S. Kozhamzharova2, R.T. Tagirova2, S.A.Mankibaeva1, A.A. Yelemes1, M.U. Aitzhan3, I.A. Myrzakhanova2
!Ai-Farabi Kazakh National University 2Asfendiyarov Kazakh National Medical University 3LLC Scientific Production and Technical Center «Zhalyn»
MORPHOFUNCTIONAL CHANGES OF HEARTS OF RATS IN THE EXPERIMENT
Data of histological changes of cardiomyocyte of rats are provided in work. Morphological indicators of heart at a hipokineziya against the background of use of dietary supplements were investigated. Hipokinesia rats is led to disrupt of blood circulation by a plethora, plasmorrhagia with the subsequent dystrophy and cellular reaction. Use of biologically active supplements promoted reduction of violations of blood circulation, dystrophic processes. These histologic researches demonstrate beneficial effect of specialized products action on structurally functional integrity of bodies and fabrics.
Key words: heart, plethora, dystrophy, cardiomyocyte, specialized products, rats.
Introduction. Now sharp depression of muscular activity of the person in the course of work and everyday life becomes perceptible. The hypokinesia turned into one of the current problems of the present demanding acceptance of immediate measures on its prophylaxis. In this regard studying of mechanisms of hypokinetic disorders, development of methods, prophylactics and corrections of consequences of a hypokinesia gain great social value. The scientific and technological revolution which now turned into constantly accruing scientific and technical progress, which declared infinite power and force of human mind at the same time put the person in conditions of a social hypokinesia [1-13].
Hipokinesia is the lowered physical activity. Until recently medicine, biologists almost didn't pay attention to hipokinesia questions. Experts in the field of space medicine as the hipokinesia is one of the factors inherent in space flight began to deal with a problem of a hipokinesia in the last 25 years actively. The sufficient activity of skeletal muscles is necessary for ensuring normal activity of a human body. Reorganization of metabolic processes in an organism at various stages of space flight causes change of requirement of an organism in a number of feedstuffs and energy. Studying and use of specialized products will allow to reduce the adaptation period, to improve the general condition of a human body and animals. Development of products of the directed preventive action for correction of a basic food allowance of people substantially will promote increase in working capacity, adaptation opportunities
of an organism and decrease in negative impact on an organism of various ecological factors [14-22]. Development of food is priority for the Republic of Kazakhstan and is of great interest to the foreign states.
Material and technique of a research: For identification of adaptic opportunities of the rats who received a hypokinesia with use of a specialized radio tire-tread product " TanSaulesy " within 30 days pilot study on 15 experienced puberal rats -males of the line Vistar, trimensual age with the initial body weight of 180-220 grams was conducted. During the experiment all animals were in identical standard conditions of a vivarium. The decapitation of animals was carried out with use of a narcosis to strictly fixed time - between 9 and 11 o'clock in the morning. An object of a histological research were the main populations of cells of a myocardium.
All experiment was made within 30 days, all animals were parted on 3 gruppy:1 group of animals - control; The 2nd group of animals contained in hypokinesia conditions within 30 days; The 3rd group of animals a hypokinesia + a radio tire-tread product "Tan Saulesy" within 30 days; Experimental animals received Water beyond all bounds.
Results of researches their discussion. Histological studying of microdrugs of a myocardium control rats on semi-thin sections showed that the muscular cover of heart is formed by cross-striped muscle cells. Squared cordial myocytes. In the central part of a myocyte 1-2 cores of the oval or extended form are located. Myofibrils lie strictly rectilinearly (figure 1).
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Figure 1 - Histological structure of heart of rats of control group. Semi-thin cut.Coloring hematoxylin - eosin.
Magnification.x 400
Vestnik KaxNMU №2-2018
At a morphological research of heart of rats of the second group it was revealed that the structure of a structure and an arrangement of muscle fibers aren't kept. On histologic medicines cross painting layers of fibers of the friable connecting fabric containing a large number of vessels indistinctly came to light. Often in the same capillary thinnings and swellings of a wall come to light. At the same time nucleus and cytoplasm of endotheliocytes can bulk up, blocking a microvessel gleam. Process of the microvesicle transport is broken, the big
congestion the pinositosis of vesicles in cytoplasm of endotheliocytes is observed that can be characterized as stagnation of a vesicle structure , the expressed intermuscular has swelled (figure 2). Convincing damage of the fact that the gipokineziya leads to morphostructural violations of blood capillaries of a myocardium are changes of structure of endotheliocytes.
Histostructure of a myocardium of rats of the second group who were exposed to hypokinesia a within a month.
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Figure 2 - Intramuscular edema. Semi-thin section Eosin-hematoxylin coloring. Magnification x 200
Changes in microstructure of investigated organs were observed in investigation of heart of animals that went through
hypokinesia within a month. On histological preparations of heart we can observe expressed muscular edema.
Figure 3 - Interstitial edema with vascular fullness.Semi-thin section. Coloring of hematoxylin-eosin. Magnification x 400
As a result of a research of the animals that received a hypokinesia and a radio tire-tread specialized product "Tan Saulesy" small interstitial edema with vascular fullness is noted in bodies of heart. At the same time less expressed changes of capillaries are noted in the right departments of heart, a nucleus slightly indistinct. In heart against the background of edema subcardinal hemorrhages were observed. Therefore, as a result of a research of the animals that received a hypokinesia and a radio tire-tread specialized product "Tan Saulesy" partial violation in bodies of heart is observed (figure 2). Experimental research on 15 experienced puberal rats - males of the line Vistar, trimensual age with the initial body weight of 180-220 grams morphostructural changes in heart organs, which
happened under the influence of a hypokinesia and a hypokinesia with addition of special additive, are revealed. There are no distinct changes in control rats. Delicacy, a malaise, change of behavior at rats isn't revealed, the general normal state, gross weight, pupils and a wool integument is normal, physiological deviations didn't take place. All organs are in normal state, the structure is kept. Distinct pathological and physiological changes arent revealed. More than a half of experimental animals of the second group relatively calmly endure hypokinesiaconditions, the others show excitement and even some aggression that, is obviously bound to a hypokinetic stress. The mass of the studied organs at the rats who are calmly enduring a hypokinesia-without changes, and at restless-
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decreased. The hypokinesia leads to morphological damages of system of microcirculation of heart. . At the same time the complexity and depth of the morphofunctional shifts depend both on hipokinesia duration, and on display of a hypokinetic stress. Around microvesselspericapillary edema is formed. Often in the same capillary thinnings and swellings of a wall come to light. At the same time nucleus and cytoplasm of endotheliocyte can bulk up, blocking a microvessel gleam. Process of the microvesicle transport is disrupted- the big congestion the pinocytosis vesicles in cytoplasm of endotheliocytes is observed, that can be characterized as stagnation of a vesicleformation.
The researches of the first stage enabled to make the following conclusions:
1. Hypokinesia of rats leads to disrupt of blood circulation by plethora, plasmorrhagia with the subsequent dystrophy and cellular reaction;
2. Use of biologically active supplements promoted reduction of blood circulation disrupt, dystrophic processes;
3. Favorable effect of a specialized product is connected with their positive influence on trophic of tissues, normalization of exchange processes in the conditions of a hypokinetic stress.
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Vestnik KazNMU №2-2018
З.Б. Есимсиитова1-3, А.С. Цожамжарова2, Р.Т.Тагирова2,С.А.Манкибаева1 А.А.Елемес1, М.У. Айтжан3, И.А.Мырзаханова2
1 Эл-Фараби атындагы К,азац ¥лттыц yHueepcumemi 2С.Ж.Асфендияров атындагы К,азац ¥лттыц Медицина yHueepcumemi 3«Жалын» Гылыми-eHdipicmiK техникалыц орталыгы»ЖШС
ЕГЕУЦ¥ЙРЬЩТАРГА ЭКСПЕРИМЕНТ ЖУРГ1ЗУ КЕЗ1НДЕ ЖУРЕКТЩ МОРФОФУНКЦИОНАЛДЬЩ 6ЗГЕР1СТЕРГЕ ¥ШЫРАУЫ
ТYЙiн: Бул жумыта егеу;уйрык;тардыц кардиомиоциттерiнiц гистологиялы; езгер^тершщ мэлiметтерi кел^ршген. Биологиялы; белсендi ;оспаларды ББЦ ^олдангандагы гипокинезия кезiндегi ЖYректiц морфологиялы; KepceTK^Tepi зерттелдi.Гипокинезия егеу;уйрык;тардыц ;ан айналымыныц бузылуына, ;анныц толу TYpiHe, плазморрагияга кешннен дистрофияга жэне жасушалы; реакцияга экеледь Биологиялы; белсендi ;оспаларды ;олдану ;ан айналымыныц бузылуына, дистрофиялы; процестердiц азаюына ы;пал eTTi. Гистологиялы; зерттеулердiц мэлiметтерi арнайыландырылган азы;тардыц эрекетiмен агзалармен тшдердщ ;урылымдык;-функционалдык; са;талуына ;олайлы эсер ететшдИн дэлeлдeйдi.
ТYЙiндi сездер: ЖYрeк, ;анныц толуы, плазморрагия, дистрофия, кардиомиоцит, арнайыландырылган азы;тар, егеу;уйрык;тар.
З.Б. Есимсиитова1-3, А.С. Кожамжарова2, Р.Т.Тагирова2,С.А.Манкибаева1 А.А.Елемес1, М.У. Айтжан3, И.А.Мырзаханова2
1Казахский Национальный Университет имени аль-Фараби 2Казахский Национальный Медицинский Университет имени С.Д. Асфендиярова 3ТОО Научно-производственный и технический центр «Жалын»
МОРФОФУНКЦИОНАЛЬНЫЕ ИЗМЕНЕНИЯ СЕРДЦА КРЫС В ЭКСПЕРИМЕНТЕ
Резюме: В работе приводятся данные гистологических изменений кардиомиоцитов крыс. Исследовались морфологические показатели сердца при гипокинезии на фоне использования БАДов. Гипокинезия крыс приводит к нарушению кровообращения в виде полнокровия, плазморрагии с последующей дистрофией и клеточной реакцией. Применение биологически активных добавок способствовало уменьшению нарушений кровообращения, дистрофических процессов. Данные гистологические исследования свидетельствуют о благоприятном влиянии специализированных продуктов действием на структурно-функциональную целостность органов и тканей.
Ключевые слова: сердце, полнокровие, дистрофия, кардиомиоцит, специализированные продукты, крысы
IRSTI 34.35.15
XG.A. Tussupbekova, XS.T. Tuleuhanov, *N. Ablaikhanova, 2A.I. Kydyrmanov, 3A.S. Kozhamzharova, 4A.M. Rakhmetova
1al-Farabi Kazakh National University 2CS MES RK Institute of microbiology and virology 3S.D. Asfendiyarov Kazakh National Medical University 4E. A. Buketov Karaganda State University
RESEARCH OF BIOCHEMICAL AND HEMATOLOGICAL PARAMETERS OF BLOOD OF CASPIAN SEALS
Results of studying of biochemical and hematologic indicators of the Caspian seals for the last five years (2012-2016) are presented in the article. Biochemical indicators of blood were determinedusinga biochemical BioChem SA analyzer and hematologic indicators on the automatic Siemens ADVIA 2120 analyzer. By results of a research it has been revealed, lowering of the level of urea in the first group for 65%, in the second group for 73%, in the third group for 72%, in the fourth group for 30%, Decrease in concentration of urea at intoxication indicates the pathological changes in a liver leading to urea synthesis violation. Decrease in level of urea can be caused by both a hypoproteinemia, and a hypophosphatemia. Activities of ALT, AST by 1,3 times in comparison with normal amount of transferases in blood has increased by 79%, and. Increase in activity hepatic transaminase speaks about processes of death of hepatocytes owing to chronic toxic defeat and increase in blood of level of the studied enzymes. On a hemocytogrammof animals increase of total number of leukocytes, decrease the percentage of neutrophils by 2,5 times in the fourth group, growth of percentage of lymphocytes by 30 times is revealed. In the third group decrease in neutrophils and increase of lymphocytes is noted. Increase in percentage of eosinophils in the second group up to 4,89±0,18% is revealed, at the same time their absolute contents didn't increase. In the second group the percentage and absolute maintenance of lymphocytes increases, also increase in level of monocytes and eosinophils indicates influences an organism of animals toxic or of the allergic substances.
Thus, on biochemical and hematologic indicators of blood of the Caspian seals, it is possible to draw a conclusion on toxic damage of a liver that is shown in malfunction of this body.
Keywords: Caspian seal, biochemical analysis, hematologic analysis, experiment.
Introduction. In recent years there is a problem of relationship strengthening of anthropogenic impact on the environment more
of marine mammals and the person in connection with and more sharply. Pollutants cause oppression of many major
