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8proceeding as alimentary toxinfection. Clinic ignorance the antibiotic associated syndrome or the incorrect treatment of the collateral displays caused by antibiotics, dictates necessity of transfer of the patient on stronger antibiotic, with consequences following from it.
T
he table. A comparative estimation of duration o f symptoms of disease, days (M±m).
group H A O
n =81 n=69 n=44
diarrhea 3.01±0.15 3.91±0.19 2.63±0.22
anorexia 2.86±0.13 3.14±0.15 2.92±0.15
fever 2.04±0.11 2.93±0.20 1.99±0.16
acetonuria 3.26±0.22 3.88±0.21 2.96±0.24
intoxication 2.80±0.13 3.20±0.15 3.04±0.17
Thus, nifuroxazid carrying out of antibacterial therapy is a highly effective preparation if necessary at gastroenteritis, caused by E. coli. The prescription of antibiotics should be regulated only by strict indications. Development of antibiotic associated syndrome - the indication to antibiotic cancellation. In need of continuation of antibacterial therapy transfer on nifuroxazid is preferable.
The list of quoted sources
1. Ardatsky M. fl, Rubitsin A.V., Mikushin U.P. The dysbacterios of intestines: modern aspects of studying of a problem and diagnostics and treatment principles. Therapeutic archive. 2001, №2, p.67-72.
2. Livzin M. A, Kostenko M. B. A place of probiotic therapies in treatment and preventive maintenance antibiotic associated diarrheas. The attending physician, 2010, №10, p.83-84.
3. Maev I.V., Samsonov A.N., Golubev N.N., Antibiotic associated diarrhea. Gastroenterology. The appendix to magazine «Consilium medicum». 2007, №1, with. 20-24.
4. Novokshonov N. A, Sokolov H. B, Berezhkova T.V. Clinical efficiency of antibacterial preparations at aqute intestinal infections at children. Infectious diseases. 2008, № 4, with. 58 - 62.
5. Soldatkin P. K. The antibiotic associated syndrome and alternative antibacterial therapy at children with aqute intestinal infections. Materials of IV annual All-Russia congress on infectious diseases. The appendix №1. M. 2012, t.10, p.351-352.
6. Ursova N.I. The tactics of complex correction of a dysbacteriosis of intestines at children: an optimum enterofuril place. Farmateka. 2008., №1, p.33-38.
7. Shevjakov M. A.The antibiotic associated diarrhea and intestines candidiasis. Diagnostics and treatment. Gastroenterology. 2011, № 2, with. 128-129.
8. Shulpekova J.O. The antibiotic associated diarrhea. The Russian medical magazine. The appendix. Gastroenterology. 2007, № 6, with. 8-15.
9. Spenser R.C. The role of antimicrobial agents in the etiology of clostridium dificile-associated disease. Antimicrobchemotherapy. 1998.41.21.7.
Modeling of lipid peroxidation In the conditions of «in situ»
TihanovV.I., DorovskikhV.A., AndrosovaO.G., AnokhinaRA., KodintsevV.V.
Amur State Medical Academy, Department of Pharmacology, Blagoveshchensk, Russia
Summary. The basis of these results we make the assumption that this process can influence the process of lipid peroxidation, in the content of lipid peroxidation products in the liver tissue. The test method promotes activation of peroxidation in the liver, increased levels of lipid
peroxidation products. A method of inducing lipid peroxidation in «in situ» allows for maintaining the integrity of the plasma membrane and can serve as a prototype of the model induce lipid peroxidation in «in situ».
Key words: «in situ», biological membranes lipid peroxidation, products of peroxidation (lipid hydroperoxides, diene conjugates, malonic dialdehyde).
To date, one of the main tasks is to search for Experimental Medicine antioxidants capable of quenching the energy of free radicals, thereby preventing the development of free-radical processes in a biological system. Practice shows - based compounds having antioxidant properties are structure, carriers are usually either one or more pyrimidine rings have the ability to extinguish the energy of free radicals [2].
To solve the problems associated with the search for new antioxidants, models, initiate the free-radical oxidation of lipids: 1) that, as a rule, the model «in vivo» - when in live biological object in different ways initiate the process of lipid peroxidation (cooling the animal heat, ultraviolet irradiation , the introduction of carbon tetrachloride); 2) Model «in vitro» - when in the glass, in vitro incubation medium is created with the contents of all the components involved in the formation of lipid peroxidation [example: induction askorbatzavisimogo (non-enzymatic) and NADPH-dependent (enzymatic) POL]. Based on a comparison of data obtained from the use of models «in vivo» and «in vitro», draws conclusions about the antioxidant properties of seeking funds. But in assessing the properties of antioxidants with phenolic structure, the use of models «in vivo» and «in vitro» is sometimes contradictory, and assessment of the true antioxidant properties of these compounds by the methods which we have, is difficult [1].
At the Department of Pharmacology, we propose to introduce the practice of searching the antioxidant properties of these compounds initiate the process of a new model of lipid peroxidation in the intact body - the activation model «in situ». Besides maintaining the integrity of the plasma membrane of cells with integrated structural proteins (which can be interpreted as receptor proteins) allow us to solve the problem of the pharmacological action of the mediator substances influencing the oxidation processes.
Materials and methods.
In the resulting model was solved problem related to the preservation of the integrity of the plasma membrane of liver cells. To address this issue by the bloodstream liver animal was fed by gravity saline solution into one of the cannulated mesenteric arteries of experimental animals. Mesenteric artery enters the portal sine animal liver and by a pressure difference created by the mass liquid in a glass container connected to the atmospheric air and lifted to a certain height fiz.rastvor flows by gravity through the vasculature of the liver tissue to a cannula extending from the right venous Sine animal's heart. To prevent the destruction of the osmotic membrane of hepatocytes in fiz.rastvor, heated to 37 degrees, with the creation of added KCl molar concentration of KCl 0,15 M. For the obstacles out of the cannulated probe soft tissue on them ligature. Empirically, it was determined the time during which we could make the circulating fluid of the bloodstream - this figure was determined in 2:00. After 2 hours of experimental liver washed with Tris buffer (pH 7.4) to a light color and frozen coffee. After defrosting liver homogenates prepared. Homogenates of liver lipids were extracted by the Folch and Bligh-dialers. In the fraction of total lipids methods Stalnaya I. (1977) and Romanova A. determined diene conjugation and general hydroperoxide [4, 5]. The content of malondialdehyde was determined by reaction with thiobarbituric acid [3].
Results and discussion.
Experimental studies carried out at the Department of Pharmacology previously been identified: the induction of lipid content DK, HP, MDA in the tissues can dynamically change with the level of DK, HP in general lipids can be reduced, and the level of MDA in the aqueous phase of tissue - increased in result, we are talking about speeding up the process of lipid peroxidation. Certainly, an increase in lipid peroxidation process we are talking about and in case of increase or
only DC or HP, as an increase in one of the components of lipid peroxidation products, as we believe, indicative of activation of lipid peroxidation in the tissues. The results obtained in this experiment indicate if the content of diene conjugates (DC) lipid hydroperoxides (HP) and malondialdehyde (MDA) in the group of animals, the "control" (intact) for taking the original data, the experimental group of animals (in this group of animals held by the vascular bed of the liver fiz.rastvor + 0.15 M KCl for 2 hours) DK content decreased 1,34 times, 1,18 times the HP, and the content of MDA increased by 53,73%. In the next phase of the experimental work to fiz.rastvoru with 0.15 M KCl, we added a solution of H2O2 (its molar concentration in the bloodstream was 5 nM). When driving fiz.rastvora with 0.15 M KCl and 5 nM solution of H2O2 in the liver tissue we observed the following effects: increased content of DC by 31,7%, MDA - by 36,6%, but we had a decrease in the HP 40%.
Thus, on the basis of these results we make the assumption that this process can influence the process of lipid peroxidation, in the content of lipid peroxidation products in the liver tissue. Conclusions.
1. The test method promotes activation of peroxidation in the liver, increased levels of lipid peroxidation products.
2. A method of inducing lipid peroxidation in «in situ» allows for maintaining the integrity of the plasma membrane and can serve as a prototype of the model induce lipid peroxidation in «in situ».
References:
1. Vladimirov, Yu.A. Free radicals in biological systems / Yu.A. Vladimirov // Soros Educational Journal ISSEP. - 2000. - T. 6. - № 12. - P. 13 - 19.
2. Kruglova, O.G. Comparative characteristics of the effect of dihydroquercetin and vitamin E on the products of peroxide oxidation of lipids in the blood of laboratory animals by exposure to cold / O.G. Kruglova // Bulletin of physiology and pathology of respiration. - 2011. - Vol. 40. - P. 71-73.
3. Stalnaya, I.D. Method for the determination of malondialdehyde with thiobarbituric acid / I.D. Stalnaya, T.G. Garishvili // Modern techniques in biochemistry. - Moscow: Medicine, 1977. -P. 66 - 68.
4. Stalnaya, I.D. Method for determination of higher unsaturated conjugated diene fatty acids / I.D. Stalnaya // Modern techniques in biochemistry. - Moscow: Medicine, 1977. - P. 63 - 64.
5. Romanova, L.A. Method for the determination of lipid hydroperoxide with ammonium thiocyanate / L.A. Romanova // Modern techniques in biochemistry. - Moscow: Medicine, 1977.- P. 64 - 65.
Optimal ratio of the component composition for the foundations for a dairy -
vegetable products
UtochkinaEA., BatalovaT.A., KupriyanovaGA, KokinaT.V.
Amur State Medical Academy, Blagoveshchensk, Russia
Abstracts: In the article the urgency of the decision on creation of technologies of dairy products enriched with functional components that contribute to human adaptation to unfavorable environmental conditions has proved. Component of the basics of composition has experimentally determined. We studied the influence of a dose of plant component on the process of fermentation mixture.
Keywords: Functional nutrition, dairy and vegetable mixture, fermented product, component composition.
