Научная статья на тему 'Metal nanostructures optimized for plasmonic enhancement of chemiluminescence yield of standard biocompatible chemiluminophores'

Metal nanostructures optimized for plasmonic enhancement of chemiluminescence yield of standard biocompatible chemiluminophores Текст научной статьи по специальности «Нанотехнологии»

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Текст научной работы на тему «Metal nanostructures optimized for plasmonic enhancement of chemiluminescence yield of standard biocompatible chemiluminophores»

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ALT'23 The 30th International Conference on Advanced Laser Technologies

LD-I-11

Metal nanostructures optimized for plasmonic enhancement of chemiluminescence yield of standard biocompatible

chemiluminophores

A.V. Palekhova1, D.R. Dadadzhanov1, T.A. Vartanyan1

1-International Research and Educational Center for Physics of Nanostructures, ITMO University, 49 Kronverkskypr., St. Petersburg 197101, Russia

Email: tavartanyan@itmo.ru, daler.dadadzhanov@gmail.com

Chemiluminescence phenomena has been widely used in the fields of medicine, chemistry and biology. It allows to detect extremely weak emission arising during chemical or biochemical reactions of chemiluminophores with reactive oxygen species [1]. The use of chemiluminescent research methods allows to successfully solve many theoretical and practical medical and biological problems. This method requires no special laboratory conditions and special preparation of the material for analysis, it is sensitive, reliable, and meets the requirements of express research methods. Unfortunately, the luminol chemiluminescence yield is large only in media with high pH levels, while its application in media with neutral, pH=7, and lower pH levels is also desirable [2]. To overcome this problem, the localized plasmon resonance in metal nanoparticles can be employed to speed up the radiative transitions and, in this way, to enlarge the chemiluminescence yield [3,4]. The enhancement factor is highly dependent on the nanoparticles size and shape that determines the spectral position of the localized plasmon resonance and its overlap with the luminol luminescent band as well as on their concentration that determines the mean distance between the luminol molecule and the nearest metal nanoparticle. Hence, a broad search for the optimum values of all these parameters is necessary to achieve the maximum enhancement factor.

The effect of the presence of silver nanoparticles on the luminol chemiluminescent intensity we measured in a specially designed microfluidic chip. The chemiluminescence spectra was measured by stationary spectrofluorometer with Xe-lamp-off, while the decay of luminol luminescence was measured with a homemade photon counter system. Silver nanoparticles were prepared by two different methods: laser ablation in water and chemical citrate-reduction method. The laser ablation method has a significant advantage over the chemical method, since the result is a colloidal solution of particles free from surfactants, while in the chemical method the surface of the nanoparticles is covered with the substances not always desirable. In the search for optimum conditions, we have studied the dependence of the luminol chemiluminescence on the pH of the medium, the nanoparticles concentration, the thickness of the shell around nanoparticles as well as on the extent of the spectral overlap between the silver nanoparticle plasmon band with the emission band of luminol. As a result of this search, about 5-fold enhancement of luminol chemiluminescence in the presence of silver nanoparticles fabricated by the laser ablation method was obtained in the media with the pH as low as 5.

This work was supported by the Russian Science Foundation (Project 23-72-00045).

[1] S. Bedouhene, F. Moulti-Mati, M. Hurtado-Nedelec, P. M. C. Dang, J. El-Benna, Luminol-amplified chemiluminescence detects mainly superoxide anion produced by human neutrophils, Am. J. Blood Res., vol. 7, pp. 41 - 48, (2017).

[2] P. Khan, D. Idrees, M.A. Moxley, J.A. Corbett, F. Ahmad, G. von Figura, W.S. Sly, A. Waheed, M.I. Hassan, Luminol-based chemiluminescent signals: clinical and non-clinical application and future uses. Appl. Biochem. Biotechnol., vol. 173. pp. 333-355, (2014), doi: 10.1007/s12010-014-0850-1.

[3] A. Karabchevsky, A. Mosayyebi, A. V. Kavokin, Tuning the chemiluminescence of a luminol flow using plasmonic nanoparticles, Light: Sci. & Appl., vol. 5, pp. e16164-e16164, (2016), doi.org/10.1038/lsa.2016.164

[4] D. R.Dadadzhanov, I. A. Gladskikh, M. A. Baranov, T. A. Vartanyan, A. Karabchevsky, Self-organized plasmonic metasurfaces: The role of the Purcell effect in metal-enhanced chemiluminescence (MEC), Sen. & Act. B: Chemical, vol. 333, pp. 129453-1 - 129453-10, (2021), doi.org/10.1016/j.snb.2021.129453

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