CC BY
5The 30th International Conference on Advanced Laser Technologies B-I-35
ALT'23
Metal clusters for biomedical application
Fu WANG1
Med-XResearch Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, China
Fluorescent metal clusters, featured by their subnanometer size, good quantum yield and photostability, low toxicity, high extinction coefficients, large Stokes shift, tuneable emission spectrum, and unobtrusive blinking on all relevant time scales, fill a void between organic dye and much larger semiconductor-based probes. Herein, we prepared several kinds of metal clusters and used for superresolution microscopy, two-photon microscopy and correlated microscopy, respectively. Furthermore, the metal clusters were also been applied for ferroptosis Therapy.
First, we prepared an aptamer functionalized silver clusters for STED microscopy. Specifically, the aptamer functionalized silver clusters were constructed by linking the anti-proliferative G-rich aptamer AS1411 with a single stranded CCCTTAATCCC DNA oligomer. Figure 1 clearly shows that the AS1411 functionalized silver clusters can bind to the Hela cells. Nucleolin protein distribution in Hela cells demonstrated in STED microscopy with resolved resolution, proving that the aptamer functionalized Ag cluster is a promising probe for STED microscopy.
Further, near-infrared emitting bi-metallic gold/silver nanoclusters with an excellent large Stokes shift around 330 nm were manufactured through one-pot synthesis. The gold/silver nanoclusters exhibit strong NIR fluorescence due to the silver effect, and can be applied as a two-photon fluorescent contrast agent for in vivo imaging. Figure 2 showed the two-photon fluorescence imaging at different depths of the mouse rear paw.
Figure 1: Confocal and STED images of AS1411-A5-AgNCs labeled Hela cells. STED power is 80 mW.
Figure 2: Two-photon confocal fluorescence images of the rear paw of a mouse at different depths.
[1] Peng, Y.; Gao, L.; Pidamaimaiti, G.; Zhao, D.; Zhang, L.; Yin, G.; Wang, F. Facile Construction of Highly Luminescent and Biocompatible Gold Nanoclusters by Shell Rigidification for Two-Photon PH-Edited Cytoplasmic and in Vivo Imaging. Nanoscale, 14 (23), 8342-8348, 2022.
[2] Peng, Y.; Huang, X.; Wang, F. Near-Infrared Emitting Gold-Silver Nanoclusters with Large Stokes Shifts for Two-Photon in Vivo Imaging. Chem. Commun., 57 (96), 13012-13015, 2021.
[3] Lan, J.; Feng, B.; Wu, X.; Yang, L.; Liu, J.; Shi, G.; Wang, F. Aptamer-Modified Silver Nanoclusters for Fluorescence Detection of Intracellular 8-Hydroxydeoxyguanosine. ACS Appl. Nano Mater., 3 (2), 1332-1338, 2020.
