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Биорадикалы и Антиоксиданты 2018 Том 5, №3
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ЭКСПЕРИМЕНТАЛЬНЫЕ АСПЕКТЫ ИЗУЧЕНИЯ ЭФФЕКТОВ
БИОРАДИКАЛОВ
MEDICAL OZONE PROMOTES NRF2 PHOSPHORYLATION REDUCING OXIDATIVE STRESS AND PROINFLAMMATORY CYTOKINES IN MULTIPLE SCLEROSIS PATIENTS
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Livan Delgado-Roche , Mario Riera-Romo , Fernando Mesta , Yanet Hernández-Matos3, Juan M. Barrios4, Gregorio Martínez-Sánchez5
department of Pharmacology, Center of Marine Bioproducts, Havana 10600, Cuba. Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez 32315, Chihuahua, México.
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Pharmacy and Food Science Institute, University of Havana, Havana, Cuba. 4Laboratory of Oxidative Stress, Mexican Association of Oxidative Stress, Mexico D.F., Mexico.
5Scientific Advisor, Free lance, Ancona, Italy. Introduction
Oxidative stress and inflammation play key roles in the pathogenesis of Multiple sclerosis (MS). Different drugs have been used in the clinical practice, however, there is not a completely effective treatment. Due to its potential therapeutic action, medical ozone represents a promising approach for neurodegenerative disorders. The aim of the present study was to address the role of ozone therapy on the cellular redox state in MS patients.
Material and methods
Ozone (20 ^g/mL) was administered three times per week during a month by rectal insufflation. The effect of ozone therapy on biomarkers of oxidative stress and inflammation was addressed by spectrophotometric and ELISA techniques. Furthermore, we investigated the action of ozone on CK2 expression and Nrf2 phosphorylation by western blotting analysis.
Results
Medical ozone significantly improved (p<0.05) the activity of antioxidant enzymes and increased the levels of cellular reduced glutathione. In accordance, a significant reduction (p<0.05) of oxidative damage on lipids and proteins was observed in ozone-treated patients. As well, the levels of proinflammatory cytokines TNF and IL-1a were lower after ozone treatment. Ozone therapy incremented the CK2 expression together with Nrf2 phosphorylation in mononuclear cells of MS patients.
BHopagHKa^w u AHTHOKCHgaHTbi 2018 TOM 5, №3
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Conclusion
These findings suggest that ozone's antioxidant and anti-inflammatory effects might be partially associated with an induction of Nrf2 phosphorylation and activation. These results provide new insights on the molecular events modulated by ozone and pointed out ozone therapy as a potential therapeutic alternative for MS patients.
Key words: multiple sclerosis, ozone therapy, oxidative stress, proinflammatory cytokines, CK2 expression, Nrf2 phosphorylation
