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22
BeernuuK Ka^MMy №2-2019
UDC 591.8: 504
XM.T. Tileshova, *Z.B. Yessimsiitova, 2P. Tleubekkyzy, 3A.S. Kozhamzharova, XM.B. Assan, xO.B. Umirbekova, XB. Satybaldina
1Kazakh National University named after Al-Farabi, Almaty 2Astana Medical University 3Asfendiyarov Kazakh National Medical University
MEDICAL EFFECT OF SILYBUM MARIANUM IN LIVER DISEASES
Today, despite the enormous success in the development of synthetic drugs, a large number of medicinal preparations permitted for use in scientific medicine include active substances of higher plants. Medicinal plants are a source for studying their use as a drug, since they are little studied. It is unacceptable to use any drug without knowing all of its pharmacological effects.
In our work, we studied the mechanism of the action of a medicinal substance based on plant origin of Silybum Marianum on rat liver. The liver is a natural filter in humans and animals; it is a unique internal secretion organ capable of self-healing, regeneration. However, with prolonged exposure to negative environmental factors, toxic substances, under the influence of various diseases, the liver functions weaken, leading to pathological processes. A morphological study of rat liver in an experiment showed that the use of Silybum Marianum improves metabolic processes, helps to restore the structure, and normalizes the basic functions of hepatocyte cells. In addition, has hepatoprotective properties. Keywords: Silybum Marianum, liver, hepatocytes, necrosis, organism, trace elements, seeds, intoxication
Introduction. The liver belongs to the vital organs of the human body. It is the liver that responsible for the destruction and neutralization of poisons and toxic substances, purifying the blood from impurities harmful to health. In the liver, bile is produced, which is involved in the breakdown of fats and intestinal stimulation. Some vitamins and minerals necessary for the normal functioning of a person a deposited in the liver tissues. It is for this reason that this body needs to pay special attention, because if is functionality violated, the whole body will suffer. In order for the liver to function normally, it is must be periodically cleaned of the toxins that accumulate in it. In addition, for these purposes, a unique Silybum Marianum plant is a good example, which has a regenerating and anti is inflammatory effect on the liver, has numerous healing properties, and is ideal. In the Silybum Marianum, there are trace elements like zinc, selenium, copper, and the whole group of fat-soluble vitamins, quartering, flavolignans, and polyunsaturated fatty acids is only about 200 components. Silybum Marianum is a good antioxidant that prevents premature aging, neutralizes free radicals that destroy biological structures. The substances that the plant contains protect and regenerate liver cells. Under the influence of toxic substances in the organs, pathological processes occur, accompanied by morphological changes in the cells and tissues forming them. Disruption of the structure of one of them reflected in the functional and structural properties of other tissue and cellular systems, which leads to certain disruption of interstitial interactions. The mechanism of action of Silybum Marianum at the molecular and cellular level is very important, but it is equally important to study the morphological processes at the cellular level, since they are little studied [1-13].
In this regard, the main goal of our work was the morphological study of a medicinal plant of natural origin of Silybum Marianum, its effectiveness on the structure of the liver of rats in the experiment. Elucidation of the biological mechanisms of action of drugs based on plant origin at the intercellular and interstitial levels is of great importance for understanding the pathogenesis of various diseases.
Material and research methods. Experimental research conducted based on the laboratory of evolutionary and ecological morphology of the Kazakh National University named after Al-Farabi. In the experiment, 30 white outbred male rats of three months of age weighing 180-220 grams were used in laboratory animals. The object of the histological study was the liver of rats. The experiment lasted 30 days; the rats divided into three groups of 10 pieces each. The first control group of rats received the usual basic diet, saline in the same way as in experimental rats. In the morning, rats of the second experimental group injected with a 40% aqueous solution of ethanol at a dose of 0.2 ml / kg, 10 times diluted with water daily when administered orally for 30 days. The rats of the third experimental group also injected with a 40% aqueous solution of ethanol at a dose of 0.2 ml / kg, 10 times diluted with water and a decoction of Silybum Marianum seed powder is 0.25 g each. They were given once a day 30 minutes before meals. Histological examination of the liver of rats done according to the standard technique [14]. Viewing and photographing the obtained histological preparations carried out using a Leica DMLS light microscope with a Leica DFS 280 digital camera. The photographs obtained processed on a Pentium 4 computer.
Figure 1-2 - Silybum marianum the seeds
Results of the study. The study of the liver of rats in the control group had a classic tubular structure. The hepatocytes are large, have a clearly visible cell membrane, large rounded nuclei are located in the center of the cell, and one or two nucleoli are visible in the nuclei. Met binuclear cells. Blood sinusoids and minor vascular sclerosis are noted (Figure 1). In the cytoplasm of
individual sites of hepatocytes marked granularity. Single bile ducts found in the parenchyma. They had the correct structure in the form of tubes, covered with epithelium. Microscopic examination of the liver of rats of the second group, who received a 40% aqueous solution of ethanol at a dose of 0.2 ml / kg, 10 times the hepatocytes diluted with water were
Vestnik KazNMU №2-2019
expressed in irregular hexagonal forms, the beam structure was not preserved. In the parenchyma, binuclear cells an often found, accompanied by physiological polymorphism. There is a disturbance of cellular contacts, accompanied by a weak swelling of the stromal (Figure 4). The vessels of the parenchyma a rounded in shape, lined with a single is layer flat epithelium. In
the lumen of blood vessels, there are single cells of the bloodstream. In medium and large vessels, plethora is noted. Expansion of the perivascular space observed in the liver parenchyma, sinusoids a widened in places (Figure 5). The bile ducts a lined with single is layer cubic epithelium, many of them are deformed. Periportal sclerosis is also noted.
Figure 3 - Blood filling of sinusoids, binuclear cells. Coloring hematoxylin and eosin. Inx 400
Figure 4 - Liver dual cells; disruption of cellular contact with edema. Stained with hematoxylin and eosin. in. x400
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Figure 5 - Expansion of sinusoids and perivascular space. Stained with hematoxylin and eosin. in x100
Thus, in rats subjected to poisoning with a 40% aqueous solution of ethanol at a dose of 0.2 ml / kg, 10 times diluted with water, an uneven vascular plethora in the liver found. In the liver, stromal edema observed; partial fragmentation of hepatocytes observed. A morphological study of rats of the third experimental group, who injected with 40% aqueous solution of ethanol at a dose of 0.2 ml / kg, 10 times diluted with water and a decoction of Silybum marianum seed powder showed that the beam structure
of the hepatocytes of the liver destroyed in places. The nuclei are located in the central part of the cells. There is a slight swelling of the stromal. Liver vessels a slightly deformed, in some parts there is detachment of the epithelium, accompanied by proliferation of connective tissue in the perivascular space, as well as a strong blood filling of the vessels (Figure 6). In small vessels, stagnation of plasma and blood is noted (Figure 7), with the formation of thrombosis of small vessels.
I'igure 6 • Swelling of the struma, deformed, marked blood supply of hlood vessels Coloring hematoxylin and eosin in x 200
BecmHUK Kß^HMy №2-2019
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Figure 7 - Stasis of small vessels blood plasi
Thus, rats of the third group after the introduction of a 40% aqueous solution of ethanol at a dose of 0.2 ml / kg, 10 times diluted with water and a decoction of Silybum marianum seed powder found that partial destruction of cells observed in the liver of rats, small edema, and vascular deformity. The use of medicinal plants has a stimulating effect on the liver cells and helps to restore their structure, normalizes the basic functions of the liver and protects the hepatocytes from pathogenic action, improves metabolic processes.
Conclusion. The liver is a complex organ in its action plays an important role in maintaining a constant body temperature and produces heparin that prevents blood from clotting, and most of the proteins contained in blood plasma, participates in metabolism, neutralizes substances that are dangerous for the body, accumulates nutrients substances and vitamins, produces bile, contributing to the breakdown and absorption of fat. Morphological changes in the rat liver after poisoning with a 40% aqueous solution of ethanol at a dose of 0.2 ml / kg, in 10 times diluted with water, destructive changes of hepatocytes are observed, the inside slice of necrosis alternate manifestations are seen, as well as the cells of various sizes, including a large number of dual and multi core, kernels of various sizes, which are swollen, where the clarity of the beam structure is disturbed. Hydropic, beam and fatty degeneration have a small focal character. In various parts of the hepatic lobules, small foci of necrosis of the parenchyma met with stromal destruction. Because of the study, it is clear that poisoning of rats leads to functional disorders, as well as pathological changes in the
i. Coloring hematoxylin and eosin. In x 200
structure of the liver. When using a 40% aqueous solution of ethanol at a dose of 0.2 ml / kg, 10 times diluted with water in combination with seed powder of Silybum marianum found to have a versatile biological activity, restorative effect, antioxidants, hepatoprotective properties. Findings.
1. As a result of poisoning of the organism of experimental rats of the second group, who received 40% aqueous solution of ethanol at a dose of 0.2 ml / kg, 10 times diluted with water in the liver of rats, a number of morphological changes were found, characterized by a violation of the beam structure, changes in the number of two and polynuclear hepatocytes, the severity of vascular changes.
2. When used in rats of the third group in combination with a 40% aqueous solution of ethanol at a dose of 0.2 ml / kg, 10 times diluted with water in combination seed powder of Silybum marianum not detected in the experiment in the liver of rat's changes of destructive nature. There is an increase in adaptive capacity, that Silybum marianum has a positive effect on the liver, in particular in inflammatory processes, in poisoning. An aqueous solution of the seeds of Silybum marianum contributed to the regeneration of the liver, the elimination of destructive changes and the restoration of compensatory-adaptive reactions.
3. Silybum marianum has a diverse biological activity. Silybum marianum is a good antioxidant that prevents premature aging. It neutralizes free radicals that destroy biological structures, and has hepatoprotective properties.
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Vestnik KazNMU №2-2019
1M.T. Тилешова, 1З.Б. Есимсиитова, 2P. Тлеубеккызы, 3A.C. Кожамжарова, 1М.Б. Асан, 1О.Б. Умирбекова, 1B. Сатыбалдина
1Эл-Фараби атындагы К,азац ¥лттыц yHueepcumemi 2Астана Медицина Университетi 3С.Ж. Асфендияров атындагы К,азац ¥лттыц Медицина Унuверсuтетi
БАУЫР АУРУЛАРЫ КЕЗ1НДЕГ1 Ш¥БАР АЛАТ1КЕННЩ (SILYBUM MARIANUM) МЕДИЦИНАЛЬЩ ЭСЕР1
ТYЙiн: ^a3ipri кезде синтетикалык дэрi-дэрмектердi дайындауда кол жетизген улкен табыска карамастан, гылыми медицинада колдануга руксат етiлген дэрiлiк препараттардыц кеп бвлiгi жогары всiмдiктердiц белсендi заттарынан турады. Дэрiлiк всiмдiктер оларды дэрiлiк зат ретiнде пайдалануды зерттеу болып табылады, себебi олардыц аз зерттелгендiгiмен байланысты. Олардын барлык фармакологиялык эсерлерiн бiлмей кез келген препаратты пайдалануга болмайды. Зерттеу жумыстарында шубар алатiкен (Silybum Marianum) еамдИ шыккан дэрiлiк заттардыц егеукуйрыкка эсер ету механизмi зерттелiндi. Бауыр - адамдармен жануарлар ушш табиги сузп болып табылатындыгы мэлiм. Бул езiн-езi сауьщтыруга, жаракаттанган дененiц кайта калпына келуiне кабыетт iшкi секрецияныц бiрегей мYшесi.
Алайда коршаган ортаныц жагымсыз эсерлерi, улы заттар уза; уа;ыт эсер еткен кезде, эртYрлi аурулардыц эсерiнен бауыр функциясы элсiрейдi, бул патологиялык процестерге экелiп согады. Тэжiрибе ЖYргiзу барысында егеукуйрьщтардыц бауырын морфологиялык зерттеу шубар алатiкен (Silybum Marianum) колдану алмасу процестерiн жаксартады, гепатоцит жасушаларынын негiзгi функцияларын калпына кел^руге кемектеседi. Сонымен катар, гепатоздан коргайтын касиеттерге ие. ТYЙiндi свздер: Silybum Marianum, бауыр, гепатоциттер, некроз, агза, микроэлементтер, тукымдар, улану.
1M.T. Тилешова, 1З.Б. Есимсиитова, 2P. Тлеубеккызы, 3А.С. Кожамжарова, 1М.Б. Асан, 1О.Б. Умирбекова, 1B. Сатыбалдина
1Казахский национальный университет им. аль-Фараби 2Медицинский Университет Астана 3Казахский Национальный Медицинский Университет имени С.Д.Асфендиярова
МЕДИЦИНСКИЙ ЭФФЕКТ РАСТОРОПШИ ПЯТНИСТОЙ (SILYBUM MARIANUM) ПРИ ЗАБОЛЕВАНИЯХ ПЕЧЕНИ
Резюме: Сегодня, несмотря на огромный успех в разработке синтетических лекарств, большое количество лекарственных препаратов, разрешенных к применению в научной медицине, включает активные вещества высших растений. Лекарственные растения являются источником изучения их использования в качестве лекарственного средства, поскольку они мало изучены. Недопустимо использовать любой препарат, не зная всех его фармакологических эффектов.
В нашей работе мы изучили механизм действия лекарственного вещества на основе растительного происхождения Silybum Marianum на печень крысы. Печень является естественным фильтром для людей и животных; это уникальный орган внутренней секреции, способный к самовосстановлению, регенерации. Однако при длительном воздействии негативных факторов окружающей среды, токсических веществ под воздействием различных заболеваний функции печени ослабевают, что приводит к патологическим процессам. Морфологическое исследование печени крысы в эксперименте показало, что использование Silybum Marianum улучшает обменные процессы, помогает восстановить структуру и нормализует основные функции клеток гепатоцитов. Кроме того, обладает гепатозащитными свойствами.
Ключевые слова: Silybum Marianum, печень, гепатоциты, некроз, организм, микроэлементы, семена, интоксикация
UDC 614.2:615.277.4:665.5
K.K. Shekeyeva
Asfendiyarov Kazakh National medical university
CHARACTERISTICS OF CARCINOGENIC SUBSTANCES
This article provides a brief description about the carcinogenic substances, found in manufacturing industries, domestic conditions and in medicinal substances.
Keywords: carcinogenic factors, medicinal substances, petroleum industry, coal production
Nowadays, the carcinogenic substances are often found in medicinal substances (Valartan, Clarithromycin) and in manufacturing industries (oil production " Tengizchevroil ", Karaganda Coal Plant). This article will describe what kind of substances and in what quantity they are often found. The list of carcinogenic substances is widely known For the International Agency for research on cancer and for other organizations. However, a list of such substances used every day by man has not yet been proposed. This article will provide a
comparative analysis of carcinogenic substances found in the home, manufacturing industries and in drugs. Methods and materials: The International Agency for the study of cancer by experimental and epidemiological analysis, as well as previously known information announced the list of carcinogenic substances. In addition, each country has its own list of such substances. Among them will be given information from the Kazakhstan lists. To be more precise, a list of carcinogens found in the branches of oil production "Tengizchevroil" and in Karaganda coal plant will be given.
