CC BY
32LICHENS - ЛИШАИНИКИ
Lithographa tesserata (Trapeliaceae, lichenized Ascomycota)
new to Japan
E. A. Davydov1, 2, L. S. Yakovchenko3, A. G. Paukov4, Y. Ohmura5
'Altai State University, Barnaul, Russia 2Central Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences,
Novosibirsk, Russia
3Federal Scientific Center of East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia 4Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg, Russia 5Department of Botany, National Museum of Nature and Science, Tsukuba, Japan Corresponding author. L. S. Yakovchenko, lidiyakovchenko@mail.ru
Abstract. The genus Lithographa and the species L. tesserata are reported for the first time for Japan. The species was collected on siliceous rocks in mountain areas of Hokkaido. It is characterized by having crustose areolate thallus, black lirellate ascomata, simple hyaline ascospores and the presence of norstictic acid. Characteristic features of the species based on the Japanese material, distribution, comparison with other species of the genus are provided. In addition, a short description of a specimen of L. tesserata from Sakhalin Island and a comparison with the Japanese material are given.
Keywords: distribution, new records, taxonomy, East Asia, Hokkaido, Sakhalin.
Lithographa tesserata (Trapeliaceae, lichenized Ascomycota), новый для Японии вид лишайника
Е. А. Давыдов1, 2, Л. С. Яковченко3, А. Г. Пауков4, Й. Омура5
'Алтайский государственный университет, Барнаул, Россия 2Центральный Сибирский ботанический сад СО РАН, Новосибирск, Россия 3Федеральный научный центр биоразнообразия наземной биоты Восточной Азии ДВО РАН,
Владивосток, Россия 4Институт естественных наук и математики, Уральский федеральный университет,
Екатеринбург, Россия
5Отдел Ботаники, Национальный музей природы и науки, Цукуба, Япония Автор для переписки. Л. С. Яковченко, lidiyakovchenko@mail.ru
Резюме. Род лишайников Lithographa и вид L. tesserata впервые приводятся для Японии. Вид был собран на силикатных породах в горных районах о. Хоккайдо. Он характеризуется накипным, ареолированным слоевищем, черными, лирелловидными апотециями, простыми, бесцветными аскоспорами и наличием норстиктовой кислоты. Приведены характерные особенности вида на основе изучения материала из Японии, а также распространение и сравнение
https://doi.org/10.31111/nsnr/2022.562351
351
с другими видами рода. Кроме того, дано краткое описание экземпляра L. tesserata с о. Сахалин в сравнение с материалом из Японии.
Ключевые слова: новые находки, распространение, таксономия, Восточная Азия, Сахалин, Хоккайдо.
The genus Lithographa Nyl. (Trapeliaceae) comprises ten saxicolous species with chlorococcoid photobionts, crustose thalli, lirellate ascomata, branching and soon disappearing or sparse paraphyses, and simple or submuriform hyaline ascospores (Hertel, Rambold, 1990; Spribille et al, 2014; Wijayawardene et al, 2022).
The genus was monographed by Hertel and Rambold (1990) along with the genus Rimularia Nyl., and additional species have since been described: L. graphidioides (Cromb.) Imshaug ex Coppins et Fryday, L. olivacea Fryday, L. opegraphoides Cop-pins et Fryday, L. serpentina Coppins et Fryday, and L. skottsbergii (Zablbr.) Fryday and Coppins (Fryday, 2004; Coppins, Fryday, 2006; Fryday, Coppins, 2007). The representatives of the genus are distributed in Europe, Asia, North and South America, Tasmania and subantarctic islands (Flock, 1989; Hertel, Rambold, 1990; Lumbsch, 1997; Foucard, 2001; Fryday, 2004; Coppins, Fryday, 2006; Fryday, Coppins, 2007; Urbanavichus et al, 2008; Smith et al, 2009; Dietrich, Bürgi-Meyer, 2012; Wirth et al, 2013; Kristinsson et al, 2014; Joseph et al, 2016; McCune, 2017; Chesnokov et al, 2018; Ezhkin, Schumm, 2018; Westberg et al, 2021).
The molecular phylogenetic analysis (Spribille et al, 2014) showed that Lithographa, Ptychographa Nyl. and Xylographa (Fr.) Fr. are closely related. Xylographa is easily separated from Lithographa by its lignicolous habit and often exposed disk with a thin to moderately thickened proper exciple which is pale brown to hyaline in its inner part. Ptychographa shares the dark exciple with Lithographa, but is easily distinguished by its inconspicuous to minute granulate thallus, hymenium which is usually divided by a longitudinal ridge of a dark, sterile tissue, and occurrence on wood. Ophegrapha Ach. can also form lirellae on rock and has a very dark exciple, but the ascospores in this genus are transversely septate, and the photobiont is Trentepohlia Mart. Lithographa differs from Rimularia Nyl. by the shape of ascomata — elongated in Lithographa and orbicular in Rimularia.
During the revisionary studies of lichens of Hokkaido, we found two interesting specimens which were identified later as Lithographa tesserata (DC.) Nyl. The species has not been previously known from Japan and is reported here as a new generic and a species record. The investigation is conducted on the scope of the project of the inventory lichen flora of Japan (Paukov et al., 2021; Galanina, Ohmura, 2022; Yakovchenko et al., 2022).
Materials and Methods
All geographical coordinates are given in the coordinate system WGS 1984. Specimens collected in Hokkaido were examined morphologically using a dissecting stereomicroscope (Olympus SZX61) and a differential interference contrast microscope (Zeiss Axio Lab.A1). Anatomical investigation was undertaken using hand-cut
sections mounted in water. Measurements are given as the (extreme minimum-) minimum-maximum (-extreme maximum) observed. Lichen substances were studied using a thin layer chromatography (TLC) with solvent B' (hexane: methyl tetra-butyl ether: formic acid, 140 : 72 : 18) and a solvent C (toluene: acetic acid, 170 : 30) (Orange et al., 2001).
Results and Discussion
Lithographa tesserata is a species characteristic by its distinct areolate, whitish to pale gray-brownish thallus of plane to convex areoles up to 0.4(1.5) mm wide, black, shortly lirellate apothecia, 0.2-1.5 x 0.2-0.4 mm, with margins of a proper exciple arranged close to each other like thick black lips, and a slit-like, not opening disk. Para-physes slender, up to 1 |m wide, branched, soon disappearing or sparse. Asci 8-spored, Rimularia-type, ascospores hyaline, broadly ellipsoid to ovate, thin-walled, 9-15 x 5-8 |m. Medulla C-, K+ yellow-red (crystals), KC+ red, Pd+ orange, UV- (TLC: norstictic acid). The specimens from Japan (Fig. 1, 2) agree well with available descriptions of the species (Smith et al., 2009; Foucard, 2001; Wirth et al., 2013; McCune, 2017) differing by somewhat larger size of areoles, apothecia, and ascospores as well as the presence of a black-greenish tint on the inner edge of the exciple. Thus, the diagnostic characters are continuous crustose thallus with whitish, areolate, mostly plane
Fig. 1. Morphology of Lithographa tesserata from Japan (Ohmura 3587, TNS): thallus and
apothecia. Scale bar: 0.5 mm.
Fig. 2. Lithographa tesserata (Ohmura 3587, TNS): asci with eight non-septate ascospores. Scale bar: 10 ^m.
areoles, up to 1.4(1.8) mm wide with uneven surface, black, poorly developed hypo-thallus, only occasionally visible between the areoles; black lirellate, adnate, numerous, solitary or crowded, straight or curved apothecia, 0.3-1.6 x 0.2-0.4 with a slitlike disc. Exciple dark brown, sometimes colored greenish-black on the inner edge, pol-. Hymenium hyaline with a dark-brown epihymenium, pol-. Paraphyses thin, ca. 1 |im wide, branched. Ascospores hyaline, broadly ellipsoid, 10.0-15.0(16.5) x x 6.0-8.0(9.0) ^m. Both medulla and the upper cortex are yellow with P and red with K (norstictic acid).
In Japan the species was found on siliceous rocks, partly overgrowing saxicolous mosses at the elevation ca. 2000 m a. s. l. Worldwide the species is recorded on moist siliceous or basic rocks, especially basalt, but also on granite in exposed sites. It is a widespread but rarely collected arctic-alpine species known from North America (Flock, 1989, McCune, 2017), Europe (Foucard, 2001; Urbanavichus et al., 2008; Smith et al, 2009; Dietrich, Bürgi-Meyer, 2012; Wirth et al, 2013; Kristinsson et al, 2014; Westberg et al., 2021), and Asia where it is reported from South Siberia (Urbanavichus, Urbanavichene, 2004, Chesnokov et al., 2018) and Sakhalin Island (Ezhkin, Schumm, 2018).
A specimen of Lithographa tesserata collected in Sakhalin Island differs from the Japanese material by its darker (brownish grey) thallus composed of larger, up to 1.5(2.0) mm wide, moderately convex areoles with a smooth upper surface, distinct hypothallus on the thallus periphery, smaller apothecia 0.20-1.00 x 0.20-0.35 mm and ascospores 9.0-13.0(15.0) x 5.0-7.5 pm, narrowly to broadly-ellipsoid, with rounded to pointed one or two ends.
Among Lithographa species, L. tesserata is unique in containing norstictic acid. Other four Lithographa species with simple ascospores are separated from L. tesserata morphologically: L. graphidioides has ± orbicular ascomata with more open discs; L. opegraphoides possesses initially slit-like discs that usually expand later; L. olivacea has dark, olive-brown thallus and slit-like discs but apothecia are smaller, up to 0.6 x 0.2 mm; L. marionensis Hertel et Rambold has gray rimose-areolate thallus reacting C+ red (gyrophoric acid). The two remaining species of the genus differ mainly by their submuriform ascospores.
Specimens examined: Japan, Hokkaido, Prov. Tokachi: along the trail between Minami-numa Campsite and Kita-numa, Mt. Tomuraushi, Daisetsu Mts, Shintoku-cho, Kamikawa-gun, rocks and stonefield on N slope, 43°31'43"N, 142°50'45"E, 2000 m a. s. l., on rocks, 28 VIII 2019, Davydov 18314, (hb. Davydov et Yakovchenko); ibid., rocks and stonefield on NE slope, 43°31'49"N, 142°50'59.5"E, 2030 m a. s. l., on N exposed rocks, 28 VIII 2019, Davydov 18315 (hb. Davydov et Yakovchenko, TNS). Prov. Ishikari: around the summit of Mt. Tomuraushi, Disetsu Mts, Biei-cho, Kamikawa-gun, 43°31'38.0"N, 142°50'54.5"E, 2130 m a. s. l., on rock, 24 VII 1997, Ohmura 3587 (TNS).
Additional specimens examined: Russia, Sakhalin, Korsakovsky District: Pik Chekhova Mt, ca. 6 km E of Yuzhno-Sakhalinsk, rock "Borodavka", N and W sides, 46°59'27"N, 142°50'18"E, 910 m a. s. l., on rocks, 8 VIII 2019, Davydov 19308 (hb. Davydov et Yakovchenko).
Acknowledgments
The study of L. S. Yakovchenko was carried out within the state assignment of Ministry of Science and Higher Education of the Russian Federation (theme no. 121031000117-9).
References / Литература
Chesnokov S., Konoreva L., Paukov A. 2018. New species and records of saxicolous lichens from the Kodar Range (Trans-Baikal Territory, Russia). Plant and Fungal Systematics 63(1): 11-21. https://doi.org/10.2478/pfs-2018-0003 Coppins B. J., Fryday A. M. 2006. New or previously misunderstood species of Lithographa and Rimularia (Agyriaceae) from the southern subpolar region and western Canada. The Lichenolo-gist 38(2): 93-107. https://doi.org/10.1017/S0024282906005512 Dietrich M., Bürgi-Meyer K. 2012. Spuren des Urner Naturforschers Anton Gisler (1820-1888) im Flechtenherbar von Fintan Greter (1899-1984) am Natur-Museum Luzern (NMLU) — Lithographa tesserata (DC.) Nyl. im 19. Jh. in der Schweiz nachgewiesen. Meylania 49: 19-26. Ezhkin A. K., Schumm F. 2018. New and noteworthy records of lichens and allied fungi from Sakhalin Island, Russian Far East, II. Folia Cryptogamica Estonica 55: 45-50. https://doi.org/10.12697/fce.2018.55.06
Flock J. W. 1989. Lithographa, a lichen genus new to continental North America. Mycotaxon 34(2): 643-645.
Foucard T. 2001. Svenska skorplavar och svampar som växerpa dem. Stockholm: 392 p.
Fryday A. M. 2004. New species and records of lichenized fungi from Campbell Island and the Auckland Islands, New Zealand. Bibliotheca Lichenologica 88: 127-146.
Fryday A. M., Coppins B. J. 2007. A second species of Lithographa with submuriform ascospores. The Lichenologist 39(3): 245-250. https://doi.org/10.1017/S0024282907006810
Galanina I. A., Ohmura Y. 2022. Rinodina endospora and R. macrospora (Physciaceae, lichenized Ascomycota) new to Japan. Novosti sistematiki nizshikh rastenii 56(1): 97-102. https://doi.org/10.31111/nsnr/2022.56.1.97
Hertel H., Rambold G. 1990. Zur Kenntnis der Familie Rimulariaceae (Lecanorales). Bibliotheca Lichenologica 38: 145-189.
Joseph S., Sinha G. P., Jagadeesh Ram T. A. M. 2016. Lithographa Nyl. (Lichenized Ascomycota), a new generic record for India. Indian Journal of Forestry 39(2): 155-157. https://doi.org/10.54207/bsmps1000-2016-TNUP74
Kristinsson H., HeiSmarsson S., Hansen E. S. 2014. Lichens from Iceland in the collection of Svan-hildur Svane. Botanica Lithuanica 20(1): 14-18. https://doi.org/10.2478/botlit-2014-0002
Lumbsch H. T. 1997. Systematic studies in the suborder Agyriineae (Lecanorales). The Journal of the Hattori Botanical Laboratory 83: 1-73. https://doi.org/10.18968/jhbl.83.0_1
McCune B. 2017. Microlichens of the Pacific Northwest. Volume 2: Keys to the species. Corvallis: 755 p.
Orange A., James P. W., White F. J. 2001. Microchemical methods for the identification of lichens. London: 101 p.
Paukov A. G., Skopintseva E. E., Davydov E. A., Yakovchenko L. S. 2021. Studies of the diversity of genus Pertusaria s. l. (lichenized ascomycetes) on Hokkaido. Problemy botaniki Yuzhnoi Sibiri i Mongolii 20(1): 343-344. [Пауков А. Г., Скопинцева Е. Е., Давыдов Е. А., Яков-ченко Л. С. 2021. Изучение разнообразия рода Pertusaria s. l. (лихенизированные аско-мицеты) на Хоккайдо. Проблемы ботаники южной Сибири и Монголии 20(1): 343-344]. https://doi.org/10.14258/pbssm.2021069
Smith C. W., Aptroot A., Coppins B. J., Fletcher A., Gilbert O. L., James P. W., Wolseley P. A. 2009. The lichens of Great Britain and Ireland. London: 1046 p.
Spribille T., Resl P., Ahti T., Perez-Ortega S., Tonsberg T., Mayrhofer H., Lumbsch H. T. 2014. Molecular systematics of the wood-inhabiting, lichen-forming genus Xylographa (Baeomycetales, Ostropomycetidae) with eight new species. Symbolae Botanicae Upsalienses 37(1): 1-87.
Urbanavichus G., Ahti T., Urbanavichene I. 2008. Catalogue of lichens and allied fungi of Murmansk Region, Russia. Norrlinia 17: 1-80.
Urbanavichus G. P., Urbanavichene I. N. 2004. New and rare species of lichens for the Republic of Buryatia of the Baikal Nature Reserve. Problemy botaniki Uzhnoi Sibiri i Mongolii 3: 136-138. [Урбанавичюс Г. П., Урбанавичене И. Н. 2004. Новые и редкие виды лишайников для Бурятии из Байкальского заповедника. Проблемы ботаники Южной Сибири и Монголии 3: 136-138].
Westberg M., Moberg R., Myrdal M., Nordin A., Ekman S. 2021. Santesson's checklist of Fennoscan-dian lichen-forming and lichenicolous fungi. Uppsala: 933 p.
Wirth V., Hauck M., Schulz M. 2013. Die Flechten Deutschlands. Stuttgart: 1244 p.
Wijayawardene N. N., Hyde K. D., Dai D. Q., Sánchez-García M., Goto B. T., Saxena R. K., Erdog-du M., Selguk F., Rajeshkumar K. C., Aptroot A., et al. 2022. Outline of Fungi and fungus-like taxa — 2021. Mycosphere 13(1): 53-453. https://doi.org/10.5943/mycosphere/13/V2
Yakovchenko L. S., Davydov E. A., Ohmura Y. 2022. Candelariella xanthostigmoides (Candelariaceae, Ascomycota) — a new lichen record to East Asia from Japan. Turczaninowia 25(1): 124-128. https://doi.org/10.14258/turczaninowia.25.1.10
