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11B-I-31
Laser Tweezers and Prospects for Live Cells Study
A. Priezzhev1, A. Lugovtsov1, A. Semenov2, Kisung Lee3, P. Ermolinskiy1, A. Kapkov1
1-Physics Department, Lomonosov Moscow State University, Leninskie Gory, 1, Moscow, 119991, Russia
2- Biologic Department, Lomonosov Moscow State University, Leninskie Gory, 1, Moscow, 119991, Russia
3- Center for Soft and Living Matter, Institute for Basic Science, Ulsan 44919, Korea
th the advent of laser tweezers, for the invention of which Arthur Ashkin (USA) was awarded the L 8 Nobel Prize, optical trapping and manipulation of live cells without mechanical contact became ctically feasible. The use of laser tweezers has opened new horizons for scientific and technological ances and developments, since it has provided new opportunities for studying the many nomena in the molecular-cell interface that form the basis of living matter. However, the absence mechanical contact does not always ensure the integrity of the living cell trapped by the laser beam, :n though the laser wavelength is selected so as to be outside the absorption spectrum of the cell. is circumstance requires additional research.
3 principle of operation of laser tweezers is based on the property of a sharply focused laser beam to :rt an effect on dielectric microparticles located near the waist of this beam with a force that brings particles to an equilibrium position and holds them there. A change in the spatial position of the m waist leads to a change in the position of the trapped particle. The removal of a particle captured the laser tweezers from the equilibrium position by external forces can be calibrated so that these ces can be accurately measured in the range of 0.1-100 pN. This is the range of forces of elastic ormation of living cells and their interaction with each other. The ability to measure these forces hout mechanical contact makes it possible to study the mechanisms of their interaction at the level individual cells, which was previously impossible.
this paper, we show the possibilities of studying with the help of laser tweezers various phenomena lerlying the interaction of individual red blood cells (RBC) suspended in autologous plasma or in utions of different macromolecules, somehow affecting the properties of these cells [1-3]. We onstrate the phenomenon of synergy discovered by us in the action of various components of od plasma, which plays a significant role in the aggregation of RBC and significantly affects blood ■ocirculation. Also, we demonstrate that the microrheologic properties of RBC depend on their age, the duration of their presence in the microcirculation after they leave the blood marrow. This is ised, in particular, by the difference in adsorption of macromolecules by the membranes of RBC of ferent age and, also, by pathologic alterations [4-6]. We present our preliminary results in asuring the forces of interaction of RBC with endothelial cells in vitro ranging from 0 to 21 pN »ending on the shape of RBC (discocytes or ehinocytes), concentration of macromolecules rinogen or dextran) in the suspending medium, and preactivation of the endothelial cells with the ior necrosis factor TNF-a [7].
s work was supported by the Russian Foundation for Basic Research (grant No. 19-52-51015).
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