LASER INDUCED FLUORESCENCE (LIF) SPECTROSCOPIC INVESTIGATION OF CERVIX TISSUE FOR
THE DETECTION OF CHRONIC CERVICITIS
AJAYA KUMAR BARIK1, SANOOP PAVITHRAN M1, JIJO LUKOSE1, REKHA UPADHYA2, MURALIDHAR V PAI2, AJEETKUMAR PATIL1, ASEEFHALI BANKAPUR1 AND SANTHOSH CHIDANGIL1
department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal 2Department of Obstetrics & Gynaecology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education,
Manipal.
Email: ajaykumarbarik@gmail.com
ABSTRACT
Cervical cancer is one of the leading cause of cancer mortalities. The detection of any abnormality like chronic cervicitis (CC), low grade intraepithelial lesion (LGSIL) and high grade intraepithelial lesion (HGSIL) may prevent further progression diseases. Optical techniques are explored for the early detection and diagnosis for diseases in which laser induced fluorescence (LIF) is one of the simple methods for the detection of various types of cancers. This technique is capable of discriminating normal and abnormal tissue sample without the necessity of exogenous fluorophores. In the present work, LIF studies have been performed using cervix tissues obtained from volunteers who have undergone hysterectomy. The tissue fluorescence was acquired by exciting the sample with 325 nm He-Cd laser through a fiber optic probe. The fluorescence spectra show two distinct peaks due to collagen and NADH around 390 nm and 440nm respectively. The fluorescence intensity of collagen and NADH is different for normal and chronic cervicitis tissue. As the disease progresses, the NADH intensity increases and the collagen intensity decreases. The device has proven to be a reliable technique for the early detection, in vivo screening and surgical demarcation.
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