CC BY
14B-I-22
Intraoperative diagnosis of human brain gliomas using THz spectroscopy and imaging: a pilot study
0. Cherkasova1,2, A. Gavdush2,3, N. Chernomyrdin2'3, S.I. Beshplav2,4, I. Dolganova3,5,
1. Reshetov6, G. Komandin2, A. Potapov4, V. Tuchin7,8, K. Zaytsev2,3
institute of Laser Physics of the Siberian Branch of the Russian Academy of Sciences, Biophysics Laboratory, Novosibirsk, Russian Federation
2Prokhorov General Physics Institute of the Russian Academy of Sciences, Laboratory of Submillimeter Dielectric Spectroscopy, Moscow, Russian Federation
3Bauman Moscow State Technical University, Laboratory of Terahertz Technology, Moscow, Russian Federation
4Burdenko Neurosurgery Institute, Moscow, Russian Federation
5Institute of Solid State Physics of the Russian Academy of Sciences, Chernogolovka,
Russian Federation
6Institute of Regenerative Medicine, Sechenov First Moscow State Medical University, Moscow, Russian Federation
7Saratov State University, Department of Optics and Biophotonics, Saratov, Russian Federation 8Institute of Precision Mechanics and Control of the Russian Academy of Sciences, Saratov, Russian Federation
Gliomas form the most common type of primary brain tumors [1]. Among the prognostic factor of glioma treatment, achievement of its gross-total resection is a crucial one for reducing the probability of tumor recurrence and increasing the patients' survival. Gliomas usually possess unclear margins, complicating their gross-total resection. Existing methods, applied to differentiate between healthy tissues and gliomas, do not provide satisfactory sensitivity and specificity of diagnosis, especially for low-grade gliomas [2]. Terahertz (THz) spectroscopy and imaging [3] represent promissing techniques for the intraoperative neurodiagnosis [4,5]. In this work, we study the THz optical properties of human brain gliomas ex vivo featuring WHO grades I to IV, as well as of perifocal regions comprised of intact and edematous tissues [6]. The tissue specimens were characterized using the reflection-mode THz-pulsed spectroscopy and histology. The gelatin embedding of tissues allowed for sustaining their THz response unaltered for several hours, as compared to that of freshly excised tissues. We observed statistical difference between intact tissues and tumors. This allows to objectively uncover strengths of THz technology in the intraoperative diagnosis of human brain tumors.
This work was supported by the Russian Science Foundation (RSF), Project #18-12-00328.
References
[1] Q. Ostrom et al., Neuro-Oncology 20(Suppl. 4), iv1-iv86 (2018).
[2] T. Garzon-Muvdi et al., Future Oncology 13(19), 1731 (2017).
[3] O.A. Smolyanskaya et al., Progress in Quantum Electronics 62, 1-77 (2018).
[4] S.J. Oh et al., Biomedical Optics Express 5(8), 2837-2842 (2014).
[5] Y.B. Ji et al., Scientific Reports 6, 36040 (2016).
[6] A. A. Gavdush et al., Journal of Biomedical Optics 24(2), 027001 (2019).
