CC BY
6*
ALT'23 The 30th International Conference on Advanced Laser Technologies
LM-I-17
Intracellular trafficking using plasmon resonance in silver and gold
nanoparticles with arbitrary shape
D.R. Dadadzhanov1, N.S. Petrov1, E.S. Smirnova1, T.A. Vartanyan1
1-International Research and Educational Center for Physics of Nanostructures, ITMO University, 49 Kronverkskypr., St. Petersburg 197101, Russia
Email: daler. [email protected]
Silver and gold metal nanoparticles find widespread applications in various fields such as targeted drug delivery, photothermal and photodynamic cancer therapies, optical coherence tomography, immunoas-says, and biosensing. Extensive research focuses on the use of nanoparticles to investigate physiological structures and cellular functions both in vitro and in vivo. The interest in metal nanoparticles stems from their unique optical and catalytic properties, which are influenced by the size and shape of the particles obtained [1]. The optical properties of metallic nanoparticles are determined by the excitation of localized surface plasmon resonance. This phenomenon occurs when electromagnetic radiation interacts with collective vibrations of conduction electrons within the nanoparticle. Understanding the impact of physicochemical properties such as shape, size, surface charge, surface chemistry, and cytotoxicity of nanoparticles on endocytosis, cell uptake, and cell survival is crucial for diagnosing and treating various disorders in living systems [2, 3]. The optical properties of silver and gold nanoparticles were investigated through experimental and numerical approaches with a focus on endocytosis. The experimental study examined the impact of nutrient medium (RPMI 1640 and dMEM) on gold and silver nanoparticles produced via pulsed laser ablation and chemical methods. It was observed that silver nanoparticles obtained through laser ablation displayed certain solubility and stability, whereas nanoparticles synthesized chemically with citric acid exhibited instability and were prone to aggregation, resulting in the loss of their characteristic plasmon resonance peak. To simulate the vesicle formation during the endocytosis of plasmonic nanoparticles, a protein shell derived from bovine serum albumin (BSA) was utilized. The formation of the shell was validated by the displacement of the plasmon resonance towards longer wavelengths in comparison to pure nanoparticles, indicating the conjugation of the nanoparticle surface with the protein. In the case of silver nanoparticles obtained through both laser ablation and chemical methods, a maximum shift of 7 nm was observed, surpassing that of gold nanoparticles synthesized chemically (3 nm) or via laser ablation (5 nm). The experimental findings were further supported by numerically calculated results using COMSOL Multiphysics. This work was supported by the Russian Science Foundation (Project 22-72-10057)
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