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Journal of Stress Physiology & Biochemistry, Vol. 10 No. 3 2014, pp. 37-46 ISSN 1997-0838 Original Text Copyright © 2014 by Vijayalakshmi, Srividhya, Muthulakshmi and Satishraj
ORIGINAL ARTICLE
Induction of Oxidative Stress by Hydrogen Peroxide Treatment in Rice Genotypes to Study the Osmolyte Accumulation Pattern and Antioxidant Capacity
12 2 2 D. Vijayalakshmi *, S. Srividhya , S. Muthulakshmi and R. Satishraj
1 Assistant Professor, Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore- 641 003, Tamil Nadu, India
2 Post Graduate Scholars, Department of Crop Physiology, Tamil Nadu Agricultural University
*E-Mail: [email protected]
Received March 13, 2013
The aim of the study was to compare the rice genotypes for oxidative stress tolerance. Induction of oxidative stress, by in vivo treatment with hydrogen peroxide (H2O2) in rice genotypes to study the osmolyte accumulation pattern and antioxidant capacity was investigated. Leaf strips of uniform size from rice genotypes FL 478, IR 29,Co 43 and FR13A were subjected to various concentrations of H2O2 (0, 0.05, 0.1, 0.15 and 0.2 mM). All the four rice genotypes exhibited varied responses to proline accumulation. FL 478 and Co 43 exhibited an increase in the accumulation of proline contents initially with low concentrations of H2O2, and thereafter showed a sharp decline in proline contents with higher concentrations. Degradation of protein contents in rice leaves was observed in all the varieties and the protein contents decreased with increase in concentration of hydrogen peroxide treatment. A gradual increase in the activities of catalase and peroxidase were recorded under H2O2 treatments. Significant upregulation of antioxidant enzyme systems and slow degradation of protein contents in the tolerant genotypes (FR 13A and FL 478) play important roles in stress protection.
Key words: Catalase, Peroxidase, Proline, Oxidative stress, Rice, Soluble protein
ORIGINAL ARTICLE
Induction of Oxidative Stress by Hydrogen Peroxide Treatment in Rice Genotypes to Study the Osmolyte Accumulation Pattern and Antioxidant Capacity
1 2 2 2 D.Vijayalakshmi *, S. Srividhya , S. Muthulakshmi and R. Satishraj
1 Assistant Professor, Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore- 641 003, Tamil Nadu, India
2 Post Graduate Scholars, Department of Crop Physiology, Tamil Nadu Agricultural University
*E-Mail: [email protected]
Received March 13, 2013
The aim of the study was to compare the rice genotypes for oxidative stress tolerance. Induction of oxidative stress, by in vivo treatment with hydrogen peroxide (H2O2) in rice genotypes to study the osmolyte accumulation pattern and antioxidant capacity was investigated. Leaf strips of uniform size from rice genotypes FL 478, IR 29,Co 43 and FR13A were subjected to various concentrations of H2O2 (0, 0.05, 0.1, 0.15 and 0.2 mM). All the four rice genotypes exhibited varied responses to proline accumulation. FL 478 and Co 43 exhibited an increase in the accumulation of proline contents initially with low concentrations of H2O2, and thereafter showed a sharp decline in proline contents with higher concentrations. Degradation of protein contents in rice leaves was observed in all the varieties and the protein contents decreased with increase in concentration of hydrogen peroxide treatment. A gradual increase in the activities of catalase and peroxidase were recorded under H2O2 treatments. Significant upregulation of antioxidant enzyme systems and slow degradation of protein contents in the tolerant genotypes (FR 13A and FL 478) play important roles in stress protection.
Key words: Catalase, Peroxidase, Proline, Oxidative stress, Rice, Soluble protein
Stress often leads to the production of Reactive High salinity and submergence stress induces
Oxygen Species (ROS) such as O2- and H2O2 in plant oxidative stress by accumulation of H2O2 (Hernandez
tissues (Desikan et al., 2004). H2O2 is produced and et al., 2000; Gosset et al., 1996; Gomez et al., 1999;
accumulates, leading to oxidative stress in plants. Savoure et al., 1999). Hydrogen peroxide (H2O2) is a
versatile molecule that is involved in several cell processes under normal and stress conditions (Quan et al., 2008). H2O2 are highly reactive to membrane lipids, protein and DNA; they are believed to be the major contributing factors to stress injuries and to cause rapid cellular damage (Hariyadi and Parkin, 1993; O'Kane et al., 1996; Prasad, 1996).
Plants have evolved complex regulatory mechanisms in adapting to various environmental stresses. Recently, H2O2, in addition to being a toxicant, has been regarded as a signalling molecule (Hung et al., 2005). Therefore, the control of H2O2 concentration is critical for cell homeostasis. Plants require biochemical and molecular strategies to survive the problem of salinity. Biochemical strategies used to enhance oxidative stress tolerance in plants include synthesis of osmotic regulators and induction of oxidative enzymes and certain hormones (Nakamura et al., 2002). Under physiological steady-state conditions, there is a balance between the production and scavenging of ROS (Skopelitis et al., 2006). Enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX) and glutathione reductase (GR) (Zhang et al., 1995; Lee and Lee, 2000), and nonenzymatic antioxidants such as tocopherols, ascorbic acid (AsA), and glutathione (GSH) (Wingsle and Hallgren, 1993; Kocsy et al., 1996; Noctor et al., 1998) work in concert to detoxify ROS.
The other way by which plants adjust to any environmental stresses is by increasing their tissue osmotic potential. Thus the cells tend to accumulate osmoprotective compounds which increases the
osmotic potential of the cell. Proline is the major compound that protects cells by stabilizing proteins and cellular membranes (Kumar et al., 2003;Martinez et al, 2003).
Rice genotypes tolerant/ susceptible to salinity and flooding stress show differential H2O2 accumulation, physiological response and antioxidant activity (Lee et al., 2001; Khan and Panda., 2008; Stanisavljevic et al., 2011; Blokhina et al., 2001). H2O2 induced an increase in membrane permeability, chlorophyll damage, lipid peroxidation (Lin and Kao, 1998; Patra and Panda, 1998). However, how a plant perceives environmental changes and how it subsequently triggers signals to activate the physiological response are yet to be explored. Hence, the present study was aimed to study the effect of various H2O2 concentrations on oxidative stress, osmolyte accumulation and antioxidant activity in a salt tolerant (FL 478); salt susceptible (IR29); flooding tolerant (FR 13A) and flooding susceptible (Co 43) rice genotypes.
MATERIALS AND METHODS
Rice genotypes (Oryza sativa L.) cvs. FL 478 (salt tolerant); IR29 (salt susceptible); FR 13A (flooding tolerant) and Co 43 (flooding susceptible) were planted in earthen pots (medium size) filled with 10 kg mixture of tank silt and farm yard manure in 5:1 ratio. Each pot was fertilized with N, P, K corresponding to 150, 50, 50 kg/ha, respectively. Three seedlings were maintained in each pot. A total of sixty pots were maintained with three pots for each treatment in a variety. Plants were watered regularly. Samples for various assays/estimations were taken on 30-35 days
after sowing. Assays were performed in the first fully expanded leaves. Samples collected in ice bucket were washed with tap water and then with double distilled water. Leaf strips of uniform size were submerged in about 150 cm3 of various concentrations of H2O2 (0, 0.05, 0.1, 0.15 and 0.2 mM) in 0.1M potassium phosphate buffer, pH 7.5 contained in 250 cm3 beakers and incubated for 6 h in dark at 25o C. Samples incubated in phosphate buffer served as control. After incubation the samples were twice washed with double distilled water and soaked dry, and processed for various observations.
The soluble protein content of the leaves was determined by measuring the colour developed by the reduction of Folin-Ciocalteau reagent by the amino acids like tyrosine and tryptophan of protein, following the method of Lowry et al. (1951) and expressed in mg g-1 FW. Proline was estimated by selective extraction with three per cent aqueous sulphosalicylic acid after removing the interfering proteins. The chromophore developed, while reacting with acid ninhydrin, was estimated spectrophotometrically adopting the procedure of Bates et al. (1973) and expressed in mg g-1 FW.
Catalase activity was determined following the method of Luck (1974). One gram of the sample was macerated and extracted in 0.067 M phosphate buffer (pH 7.0). A known volume of the extract was added to the experimental cuvette containing three ml H2O2 -PO4 buffer. The time taken for per cent change in absorbance (At) at 240 nm was recorded for calculating the enzyme activity and expressed as enzyme units g-1 tissue. All the operations were
carried out at 0 - 5oC. Peroxidase activity was determined by adopting the method of Malik and Singh (1980). One gram of leaf was macerated and extracted in 0.1 M phosphate buffer (pH 7.0). A known volume of the extract was added to an experimental cuvette containing three ml phosphate buffer and 0.05 ml guaiacol reagent and then 0.03 ml of H2O2 solution was added rapidly and the increase in absorbance at 436 nm was recorded. This At in minutes was used to calculate the enzyme activity. The enzyme activity was expressed as enzyme units per litre. All the operations were carried out at 0 - 5oC.
RESULTS
Four rice genotypes differing in their tolerance behavior to salinity (FL 478 and IR 29) and submergence (FR 13A and Co 43) were subjected to oxidative stress by exposing them to various H2O2 concentrations to study the osmolyte accumulation pattern, protein degradation and antioxidant activity. The results revealed that the genotypes FL 478 and Co 43 exhibited an increase in the accumulation of proline contents with 0.5 mM H2O2 and 1 mM H2O2, and thereafter showed a sharp decline in proline contents when exposed to 0.15mM H2O2 and 0.20mM H2O2. The genotypes IR 29 and FR 13A showed a decline in proline contents with exposure to increasing concentrations of H2O2 (Table 1). Among the genotypes taken for the study, FR 13A recorded a higher content of proline (1000 ^g/g) under control conditions and was also able to maintain a higher proline content (840 ^g/g) compared to other genotypes even after exposure of 0.20mM H2O2. Statistically significant changes were observed in the
accumulation of proline contents within the genotypes and treatments.
With respect to the soluble protein contents, hydrogen peroxide treatment showed pronounced and adverse effects on soluble protein contents irrespective of the varieties taken for the study. Hydrogen peroxide treatment resulted in decrease in protein contents and the decrease increased with increase in concentration of hydrogen peroxide treatment (Fig.1). In genotypes FL 478 and FR 13A the protein content gradual decreased with H2O2 treatments (FL 478: 20.15 to 16.12 mg/g; FR 13A: 20.15 to 16.53 mg/g) while in varieties IR 29 and Co 43 the protein contents gradually decreased upto 0. 1mM H2O2 and thereafter there was a steep decline in the protein contents at
0.15mM and 0.2mM H2O2 treatments.
The antioxidant activity was studied by observing the ROS scavenging enzymes namely catalase and peroxidase. Activities of catalase and peroxidase showed increasing trends with increasing H2O2 treatments in all the varieties. FR 13A manifested higher activity of catalase and peroxidase than the other genotypes at all concentrations of H2O2 treatments. In this genotype the catalase activity ranged from 0.455 ^moles H2O2/g/s under control to 1.526 ^moles H2O2/g/s at 0.2mM H2O2 treatments (Table 2). Similar pattern was also observed with peroxidase activity where FR 13A recorded 110 enzyme units/g under control and increased upto 192 enzyme units/g at 0.2mM H2O2 treatments (Table 3).
Figure 1. Effect of Hydrogen peroxide treatment on the soluble protein contents (mg/g) in rice genotypes Table 1 Effect of Hydrogen peroxide treatment on the proline contents ^g/g) in rice genotypes
Treatments Rice genotypes
FL 478 IR 29 Co 43 FR 13A
Control 955±18.75 892±17.51 908±17.82 1000±19.63
0.05mM H2O2 986±19.36 875±17.18 926±18.18 978±19.20
0.1mM H2O2 942±18.49 855±16.78 922±18.10 972±19.08
0.15mM H2O2 915±17.96 842±16.53 911±17.88 940±18.45
0.20mM H2O2 802±15.74 692±13.58 799±15.68 840±16.49
SEd. 25.75
CD (P=0.05) 52.036*
Table 2 Effect of Hydrogen peroxide treatment on the Catalase activity (nmoles H2O2/g/s) in rice genotypes
Treatments Rice genotypes
FL 478 IR 29 Co 43 FR 13A
Control 0.422±0.01 0.399±0.01 0.402±0.01 0.455±0.01
0.05mM H2O2 0.578±0.01 0.485±0.01 0.499±0.01 0.502±0.01
0.1mM H2O2 0.644±0.01 0.594±0.01 0.591±0.01 0.679±0.01
0.15mM H2O2 0.754±0.01 0.667±0.01 0.753±0.01 0.825±0.02
0.20mM H2O2 1.322±0.03 0.699±0.01 0.984±0.02 1.526±0.03
SEd. 0.021
CD (P=0.01) 0.056**
Table 3 Effect of Hydrogen peroxide treatment on the Peroxidase activity (units/g) in rice genotypes
Treatments Rice genotypes
FL 478 IR 29 Co 43 FR 13A
Control 80±1.57 98±1.92 85±1.67 110±2.16
0.05mM H2O2 92± 1.81 104±2.04 112±2.20 125±2.45
0.1mM H2O2 130±2.55 122±2.39 135±2.65 140±2.75
0.15mM H2O2 154±3.02 136±2.67 142±2.79 174±3.42
0.20mM H2O2 175±3.44 140±2.75 160±3.14 192±3.77
SEd. 202.88
CD (P=0.01) 410.04 ns
DISCUSSION
Hydrogen peroxide is a potent cytotoxic compound produced during salinity, drought, high and low temperature stresses (Sairam and Srivastava, 2000). In order to understand the physiological mechanisms underlying salinity and flooding stress tolerance in rice, genotypes exhibiting contrasting tolerance behaviour to these stresses were taken for the study. The study clearly indicated that the genotypes varied significantly in the proline accumulation pattern and there was no uniform trends observed with the proline accumulation. The slight increase in proline, an osmoprotectant in H2O2 (0.5 and 1.0 mM) in FL 478 and Co 43 may be attributed to the free radical scavenging function of proline as reported (Smirnoff, 1993; Sairam and Srivastava, 2000; Matysik, 2002).
Enhanced accumulation of proline in FL 478 might be the basis for its salinity tolerance. These findings are in line with Yazici et al., (2007) who has reported improved salt tolerance of Portulaca oleracea L.with proline accumulation. Proline accumulation and stress tolerance correlation have been reported in different studies, and it has been observed that proline concentrations are higher in stress-tolerant plants than in stress-sensitive plants (Misra and Gupta, 2005). Although a positive correlation between abiotic stress tolerance and free proline accumulation has been reported (Martinez et al., 2003), a negative correlation between proline accumulation and submergence tolerant line (FR 13A) was observed in the present study. FR 13A showed a decline in proline contents with exposure to increasing concentrations of H2O2.
This is in line with the findings of Lutts et al.who showed that stress sensitive rice cultivars accumulated more proline than the tolerant ones.
It is known that water, salt, metal toxicity and other stress factors induced endogenous H2O2 accumulation (Upadhyay et al., 2007). H2O2 treatment of primary rice leaves induced an increase in chlorophyll, carotenoid and protein degradation in senescing leaves as observed also for other abiotic stresses (Sairam et al., 1997; Panda et al., 2002). In this study also it was found that in all the varieties taken for the study, hydrogen peroxide treatment resulted in decrease in protein contents and the decrease increased with increase in concentration of hydrogen peroxide treatment. In the stress tolerant lines (FR 13A and FL 478) the protein content gradually decreased, while in the other varieties (Co 43 and IR 29) there was a steep degradation of protein contents at 0.15mM and 0.2mM H2O2 treatments. The protein degradation in senescing leaves may be due to a cytotoxic effect of H2O2 (Mukherhee and Choudhuri, 1983; Menconi et al., 1995; Khan and Panda, 2002).
POX, CAT, SOD are the three major antioxidant enzymes responsible for scavenging, the reactive oxygen species generated via different mechanisms in plant cells (Winston, 1990). This formed the basis to study the activities of two major antioxidant enzymes POX and CAT. Activities of catalase and peroxidase showed increasing trends with increasing H2O2 treatments in all the varieties. The increased CAT and POX activities point to a signalling role of H2O2 in the induction of H2O2 synthesis detoxifying enzymes in rice leaves, as reported for other abiotic stresses (Guo
et al., 1997; Sairam and Srisvastava, 2000; Lee et al, 2001; Mittova et al., 2002). The study clearly stated that under conditions of oxidative stress, such as exposure to H2O2 the antioxidant levels increase in plant tissues and was much higher in the tolerant genotypes. Similar findings has been reported by Smith et al., 1990 with exposures to 03, SO2, heat shock or drought stresses.
From the study it is clear that induction of oxidative stress, by in vivo treatment with hydrogen peroxide in rice genotypes varying in their tolerance behaviour to different abiotic stresses (Submergence and salt stress) gave a clear understanding of the signalling role of H2O2. There was no uniform pattern in the accumulation of the osmolytes but significant upregulation of antioxidant enzyme systems and slow degradation of protein contents in the tolerant genotypes (FR 13A and FL 478) could explain the physiological basis of tolerance and play important roles in stress protection.
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