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Abstracts. PHYTOPHARM 2017
500 mg/kg bw. The inhibition of a-amylase activity by PCT and acarbose standard was dose-dependent from 0.2 to 1.0 mg/ml. The highest a-amylase inhibition was observed at 1.0 mg/ml of PCT, which was 20.63%, where acarbose standard with the similar concentration only showed 14.30% inhibition of a-amylase. The in vivo study showed that PCT at 500 mg/kg bw decreased the blood glucose level in alloxan-induced diabetic mice, and its efficacy was comparable to metformin
at 100 mg/kg bw. PCT (100 and 500 mg/kg bw) also significantly modulated the expression of diabetes-related genes (P < 0.05). In adipose and skeletal muscle tissues, PCT upregulated the expression of PPARy and Tcf7l2 genes, meanwhile MCP-1 gene expression was downregulated. In the pancreas, PCT upregulated the expression of Glut2, Capn10, and Tcf7l2 genes. These results indicate that PCT has antidiabetic potential for management of type 2 diabetes.
IN VITRO ANTIOBESITY AND ANTIDIABETIC ACTIVITIES OF ACTIVE FRACTIONS FROM ZANTHOXYLUM ACANTHOPODIUM FRUITS
© Yanti12, Riyardi Wijaya Limas2, Yenny Novita2
1 Food Technology, Faculty of Biotechnology, Atma Jaya Catholic University, Jakarta, Indonesia;
2 Biology Program, Faculty of Biotechnology, Atma Jaya Catholic University, Jakarta, Indonesia
Therapeutic strategy using spice-derived compounds which are naturally occurring phytochemicals and biomolecules may elicit anti-obesity and anti-diabetic properties. In this study, Zanthoxylum acanthopodium, known as lemon pepper, was isolated for its active fractions (protein, polysaccharide, polyphenol, and essential oil) and investigated for its actions for treatment of obesity and diabetes via regulating the secretion of pro-inflammatory mediators and lipogenic proteins in cell culture system of 3T3-L1 pre-adipocytes and Chang hepatocytes in vitro. In obesity system, ELISA profiles showed that protein fraction from lemon pepper at 1-100 ig/ml significanlty increased up to 140% of adiponectin level in 3T3-L1 adipocytes compared to the untreated cells. Protein fraction at various concentrations (1-100 ig/ml) also attenuated the expression of leptin and interleukin (IL)-6 proteins up to 30% in cells, indicating that it may be potential as antiobesity candidate by reducing and regulating caloric intake,
body weight, and lipid metabolism. In diabetic system, ELISA profiles demonstrated that all lemon pepper fractions, including protein and polysaccharide fractions at 1-100 ig/ml, polyphenol and essential oil fractions at 1-25 ig/ml, effectively reduced the expression of C-reactive protein (CRP) level up to 25% in the dose-dependent manner in Chang cells. CRP is known as one of the inflammatory factors produced by liver in an acute phase of inflammation and its concentration can increase in plasma. Meanwhile, polysaccharide (1-100 ig/ml) and polyphenol fractions (1 -25 ig/ml) were found to be active on inhibiting tumor necrosis factor (TNF)-a and monocyte chemoattractant protein (MCP)-1 levels at range of 20-45% compared to untreated Chang cells. Both TNF-a and MCP-1 are recognized as pro-inflammatory cytokines in obesity-related diseases. Therefore, selected fractions from Z. acanthopodium may possess antiobesity and antidiabetic potentials on hepatocytes and adipocytes in vitro.
ANTI-INFLAMMATORY EFFECT OF ANTHOCYANIN EXTRACTED FROM CLITORIA TERNATEA BLUE POWER PETAL IN CARRAGENAN-INDUCED RAT PAW EDEMA
© Yanti123, Yoseph Toni Wijaya2, Adi Yulandi23, Agustin Wydia Gunawan3, Devi Anggraini Ngandiri3, Dina Sabella3
1 Food Technology, Faculty of Biotechnology, Atma Jaya Catholic University, Jakarta, Indonesia;
2 Master of Science in Biotechnology, Faculty of Biotechnology, Atma Jaya Catholic University, Jakarta, Indonesia;
3 Biology Program, Faculty of Biotechnology, Atma Jaya Catholic University, Jakarta, Indonesia
Inflammation therapeutics could be targeted by non- of these drugs have been increasing. This study was steroidal anti-inflammatory drugs (NSAIDs), however, aimed to evaluate the anti-inflammatory activity of several gastrointestinal side effects caused by the use anthocyanin fraction isolated from Clitoria ternatea blue
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
vol. 15/2017/suppLeMEnT 1
