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14Impact on cytoflavin cold adaptation of animal organism
Dorovskikh V.A., Li O.N., Dorovskikh V.Y., ShtarbergM.A., SimonovaN.V., AnokhinaR. A., Kodintsev V. V.
Amur State Medical Academy, Department of Pharmacology, Blagoveshchensk, Russia
Summary:We analyzed three groups of experimental animals that are similar in terms of sex and weight characteristics, one of the groups used pharmaceutical (cytoflavin). Matched dosage of the drug has been applied in the cold model experiment. To study the effect of the drug on a warmblooded organism we investigated the antioxidant system of the body (lipid peroxidation). The results are discussed in view of performance of the two groups of animals (intact, control), and some data on the basis of literature, of which relevant conclusions made on pharmacological action of the drug in cold conditions in a warm-blooded organism.
Keywords antioxidants, cytoflavin, cold stress, lipid peroxidation, experiment.
Hypoxia is a complex functional and metabolic disorder, which is based on reduction of delivery and utilization of oxygen in the cells of the body, which may be due to dysfunction of the body: respiratory, cardiovascular system, blood transport, mitochondrial dysfunction. The mechanism of development of hypoxia, which is a mismatch between the needs of tissue oxygen delivery and its associated primarily with the violation of oxidation as a result of difficulties of electron transport in the mitochondrial respiratory chain, resulting in damage to the membranes of lysosomes with access autolytic enzymes into the extracellular space.
The complex mechanism of hypoxia in the body, multi-component system of biochemical and metabolic disease processes occurring in various pathologies, explains the difficulties in prescribing drugs that can provide protection antihypoxic cells. Antigipoksanty and antioxidants are energokorrektorami metabolism that perform tasks correction respiratory chain function and other metabolic processes of supplying energy substrates.
Cytoflavin a balanced complex of two metabolites (succinic acid, riboksin) and two coenzyme vitamins (riboflavin mononucleotide - Vitamin B2, nicotinamide - Vitamin PP)
All components cytoflavin are natural metabolites of the body that are utilized by cellular structures involved in redox reactions, reduce lipid peroxidation, activation of the antioxidant defense system, which leads to the normalization of metabolic processes in the body. Metabolic energokorrektsiya, anti-hypoxic and antioxidant activity of the drug, which determine the pharmacological properties and therapeutic efficacy of the components, due to the complementary action of succinic acid riboksina, riboflavin and nicotinamide.
Taking into account the above, the purpose of our study was to investigate the influence of cytoflavin on the intensity of lipid peroxidation (LPO) and antioxidant system (AOS) under cold stress.
Materialsandmethods:
Cytoflavin antioxidant properties were studied in laboratory animals (white rats-males), to modify the content of lipid peroxidation products: hydroperoxide, conjugated diene, malondialdehyde, and components of the antioxidant system: ceruloplasmin, catalase, vitamin E, glucose-6-phosphate dehydrogenase in serum Blood exposed to prolonged cold exposure. Statistical processing of biochemical data was performed using the parametric method using «t» t-test. To study the effect of the drug on the body of experimental animals was modeled cold model experiment, which involved three groups of animals at the same time, each was on 30 rats weighing 200 g The experimental animals were kept under standard conditions in the animal facility of the Amur State Medical Academy, under the natural light regime without limiting access to food and water. Animals daily at one and the same time for 3 hours was placed in firm klimatokameru "Fentron" (Germany) at -15 ° C for 21 days. In the camera provides air flow to prevent hypoxia and
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creates a constant cooling. To evaluate the antioxidant activity determined by products of lipid peroxidation and antioxidant components of the system. The animals produced by decapitation at 7, 14, 21 days of the experiment. Experimental setup: 1 - intact group - animals were in normal conditions vivarium, 2 - the control group - animals subjected to prolonged cooling using klimatokamery "Fentron" (Germany) at -15 ° C for 3 hours daily for 21 days, 3 - treatment group -animals immediately before cooling for 21 days cytoflavin intraperitoneally administered 100 mg / kg.
The results of the study:
It has been found that the effect of low temperatures on rats is accompanied by activation of lipid peroxidation products and their accumulation in the blood of animals cooled. It is proved that the activation of lipid peroxidation during cold exposure in rats develops in the stress and exhaustion of the antioxidant system of blood, characteristic changes which include a reduction in the activity of glucose-6-phosphate dehydrogenase, catalase and ceruloplasmin, and a decrease in vitamin E. In cold conditions, the experimental model cytoflavin exhibit pronounced antioxidant properties, which results in reducing the amount of lipid peroxidation products in the blood cooled animals. This is an increase of antioxidant activity of components of the system (ceruloplasmin, vitamin E). In turn, the investigation of the activity of antioxidant enzymes enabled state improving catalase and glucose-6-phosphate dehydrogenase, 7, 14 and 21 days of the experiment, but the differences were not significant performance data.
In addition, there is a direct relationship antioxidant action cytoflavin Od duration of its use. The drug begins to act on the 7th day of the experiment, and the maximum efficiency is observed by day 14 of the experiment.
In general, the first to prove the effectiveness of the correction of cold stress in experimental drug administration "cytoflavin", which allows us to recommend the drug for use in the treatment of diseases occurring against the backdrop of the lipid peroxidation. Findings:
1. First experimental proof of the possibility of correction of cold stress by introducing a drug "cytoflavin" containing succinic acid.
2. Intraperitoneal administration cytoflavin laboratory animals (rats) at a dose of 100 mg / kg decreased the intensity of lipid peroxidation of membranes induced by prolonged cold exposure, restoring the steady-state level of peroxidation products on the background of significant increase in activity of the main components of the AOC (ceruloplasmin and vitamin E).
3. Our study indicated recom - General to recommend cytoflavin as an antioxidant, as well as a regulator of adaptive reactions of the body at impact - action of low temperatures.
References:
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