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9IMAGING BY PHOTOACOUSTIC MICROSCOPY AND TOMOGRAPHY WITH PLASMONIC TIN
NANOPARTICLES
ANDERSON S. L. GOMES13 AVISHEK DAS 1, ARTHUR CESARE 1, ANTON A. POPOV 2, ANDREI
PASTUKHOV 2 AND ANDREI KABASHIN 23
1Departamento de Física, Universidade Federal de Pernambuco, Recife, 50670-901, PE, Brazil; avishek55das@gmail.com (A. D); arthurcmvp@gmail.com (A. C); anderson.lgomes@ufpe.br (A. G) 2AixMarseille University, CNRS, LP3, 163 Ave. De Luminy, Case 917, 13288 Marseille, France; kabashin@lp3.univ-
mrs.fr (A.V.K.)
3Bio-Nanophotonics Laboratory, MEPhI, Institute of Engineering Physics for Biomedicine (PhysBio), 31 31 Kashirskoe
sh., 115409 Moscow, Russia
ABSTRACT
Photoacoustic imaging (PAI) is enabled by a hybrid of optical excitation and acoustic detection which comprises both the advantages of high optical contrast and ultrasonic resolution. Some PAI techniques are mostly advantageous for preclinical or clinical non-invasive biomedical imaging. In this talk, we shall highlight the basics and state-of-the-art in PAI methods with particular emphasis on the use of exogenous contrast agents. We will describe how we have exploited Titanium Nitride (TiN) nanoparticles for PAM (Photoacoustic Microscopy) and PAT (Photoacoustic Tomography) prepared by the laser ablation technique [1]. Following a structural and optical characterization, TiN nanoparticles ranging from 50-100nm in size were employed. The absorption spectra of the nanoparticles confirmed the presence of a broad plasmonic band covering both parts of the visible and near-infrared (NIR) spectrum. Recent characterization of the nonlinear optical properties of such nanoparticles has been recently published [2]. For the photoacoustic imaging, a labmade setup was developed by employing a Q-switched Nd: YAG laser (fundamental and second harmonic), high-frequency ultrasonic transducer, customized two-dimensional (2D) scanning stage and real-time data acquisition computer program. The PAM system was used to image the TiN nanoparticles with a resolution of ~ 50 ^m, underneath 1 mm chicken breast tissue. For the PAT results, lower resolution (~400 ^m) was measured, but the penetration depth of 2cm was observed. Future applications will be discussed.
The authors thanks CNPq, FACEPE and CAPES for financial support for this work. REFERENCES
[1] Popov, A.A., Tselikov, G., Dumas, N. et al. Laser-synthesized TiN nanoparticles as promising plasmonic alternative for biomedical applications. Sci Rep 9, 1194 (2019).
[2] Melissa E. Maldonado, Avishek Das, Anderson S. L. Gomes, Anton A. Popov, Sergey M. Klimentov, Andrei V. Kabashin, Nonlinear Photoacoustic Response of Suspensions of Laser-Synthesized Plasmonic Titanium Nitride Nanoparticles, Opt. Lett. 45, 6695-6698 (2020).
