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MMP-1, MMP-8, MMP-13 INHIBITORY ACTIVITIES OF METHANOLIC EXTRACTS OF CULTIVATED ACHILLEAMILLEFOLIUM, ACHILLEAFILIPENDULINA, MENTHAPIPERITA AND SALVIA OFFICINALIS AND THEIR CORRELATION WITHMETABOLOMIC PROFILING
© Ekrem Mural Gonulalan1. Emirhan Nemutlu2, L. Omur Demirezer1
1 Hacettepe University, Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey;
2 Hacettepe University, Faculty of Pharmacy, Department of Analytical chemistry, Ankara, Turkey
Sun light (UV radiation) increase MMP (matrix metalloproteinase) enzyme production and play a role on photoaging in human skin [1]. In this study, MMP-1, 8, 13 inhibitory effect of methanolic extracts from cultivated Achilleamillefolium, Achilleafilipendulina, Menthapiperita and Salvia officinaliswereinvestigated at 100 |ig/mL concentration. The results were (Inhibition % at 100 |ig/mL):
The metabolomicsanalysis of four cultivated plants have been performed by using gas chromatography-mass spectrometry (GC-MS). After deconvolution and aligned of the chromatograms, 161 mass spectral features have been detected and 88 of them were annotated using retention index libraries. The univariate (t-test), multivariate (PCA and PLS-DA) and correlations
analysis were applied to evaluate correlation between metabolomic levels and MMP inhibitory activity.
It is very well know the whole metabolite poolmay affect the activity of the plant because of synergy and antagonism mechanism between the metabolites and instead of single metabolite. Therefore, we compared the whole metabolomic profile of different plants and MMP inhibitory activity in order to find the metabolites that have stimulatingor inhibiting activity. The four plants showed similar metabolomic profiling. We found 94 metabolites have negative correlation between metabolites levels and MMP inhibitory activity, while 67 of highly positive correlation (r > 0.70). 10 metabolomics were found negatively correlatedwhiletwo of the metabolomics positively correlated in all plants for MMP-1, MMP-8, and MMP-13 inhibitory activities.
References:
1. Lahmann C, Young AR, Wittern KP, Bergemann J,
2001. Photochem Photobiol, 73:657-663.
A. millefolium A. filipendu-lina M. piperita S. officinalis
MMP-1 73.2 66.95 72 . 24 70.99
MMP-8 74.47 72.89 78 . 25 80.51
MMP-13 77.9 77.32 90.64 85.53
HERBAL REMEDIES FOR HYPOACTIVE SEXUAL DESIRE BASED ON PERSIAN MEDICINE
© Reihaneh Moeini, Narjes Gorji, Zahra Memariani, Seyyed Ali Mozaffarpur
Traditional Medicine and History of Medical Sciences Research Center, Babol University of Medical Sciences, Babol, Iran
Nowadays industrial lifestyle beside cardiac, metabolic and psychiatric disorders results in high prevalence of sexual impotence. Traditional medicine like Persian Medicine (PM) are valuable resources of remedies for improving sexualpotency which can reduce the burden of this disorder.
Reports on pharmacological therapy of sexual potency that described in medieval PM, were gathered and analyzed from selected medical and pharmaceutical textbooks of PM. The search of databases such as PubMed, EMBASE/ Excerpta Medica, Science direct, Scopus, Google scholar, Web of science was performed to reconfirm the efficacy of PM remedies in conventional medicine.
44 medicinal herbs were found for increasing libido and sexual potency in PM medical texts such as gooseberry, mango, hazelnut, carrot, chickpea, cinnamon, turnip, clove, galangal, almond etc [1]. Modern studies have shownimpact of some of them on improving sexual hormone level (Ocimum basilicum [2], Cinnamomum zeylanicum [3], Corylus avellana [4]), menopausal symptoms (Citrus Aurantium
[5]), premature ejaculation and erectile dysfunction with increasing intracavernous blood flow (Phyllanthus emblica
[6] and Pistacia vera [7]). Some of them no evidences was found related to effect on sexual function.
Many medicinal herbs have been introduced to boost sex drive in PM but new studies are preliminary about
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] vol. 15/2017/suppLement 1
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many of them. Considering the phrases used to describe the impact of these drugs, it seems some of them have temporary and some have lasting effects. Most of remedies have been advised to use with honey or yolk for increasing their effects. More studies would be necessary for evaluation of safety and efficacy of these herbs.
References:
1. Khorasani, MH Aghili, 2001.
2. Khaki A, Azad FF, Nouri M, Khaki AA, 2011. J of
Medic Plants Res. 5(18):4601-4604.
3. Jahromi VH, Parivar K, Forozanfar M, 2011. Iranian Journal of Applied Animal Science, 1(2):99-103.
4. Abeer E, Amr AR, 2013. Pak. J. Nutr. 12:229-38.
5. Choi SY, Kang P, Lee HS, Seol GH, 2014. Evid Based Complement and Alternative Med. 2014:796518.
6. Sansalone S, Russo GI, Mondaini N et al, 2016. Arch Ital Urol Androl, 88(3):171-176.
7. Aldemir M, Okulu E, Ne§elioglu S et al, 2011. Int J of Impot Res. 23(1):32-8.
VIOLA SYRUP AS AN ANTITUMOR FORMULA IN PERSIAN TRADITIONAL MEDICINE: IN-VITRO STUDY
© Parvin Pasalar1, Narjes Gorji2, Reihaneh Moeini2, Maliheh Paknejad1,Javad Hashemi1, Hossein Rezaeizadeh1, Mohammad Kamalinejad3, Siamak Salami3, Esmail Nazem 1
1 Tehran University of Medical Sciences, Tehran, Iran;
2 Traditional Medicine and History of Medical Sciences Research Center, Babol University of Medical Sciences Babol, Iran;
3 Shahid Beheshti University of Medical Sciences, Tehran, Iran
Complementary and Alternative Medicine (CAM), such as Persian medicine (PM) is a precious source of methods in the management of cancer. In this study one of the prescriptions of Persian Medicine was tested on a breast adenocarcinoma cell line (MDA- MB-468).
V. odorata L. was prepared as syrup and its anticancer properties was studied using flow-cytometry and MTT assay in compared to aqueous extract and sucrose alone. Two different cell lines were used in this study: breast cancer cell line (MDA-MB-468) and normal fibroblast cell (AGO-1522).
MTT and flow-cytometry assays showed a dose-dependent cytotoxic activity of V. odorata L.on MDA-MB-468, regardless of the preparation method. In the MTT assay; crude extract of v. odorata L., with or without
sucrose, had potent cytotoxic effect (IC50:1.36 mg/ ml and 0.23 mg/ml respectively) and no efficacy on the normal cells in these concentrations (P > 0.05). In flow-cytometry assay 2 mg/ml of viola syrup was the lowest concentration that significantly increased the cell apoptosis compared with negative control. The percentage of cell apoptosis was higher when cells were treated with 4 mg/nl of viola syrup compared to the positive H2O2 control (57.1% vs. 51%).
This study revealed that the cytotoxic activity of V. odorata L. was lower in the presence of sucrose. To better understand the potential benefits of this PM recommendation, more studies on the different two forms are required in gastric absorption, side effects and patient compliance.
ANTI-TUMOR ACTIVITIES OF PANAX QUINQUEFOLIUS SAPONINS AND POTENTIAL DIAGNOSTIC MARKERS IN PROSTATE CANCER
© Shan He, Fangqiao Lyu, Lixia Lou, Lu Liu, Songlin Li, Tianchi Jiang, Johannes Jakowitsch, Yan Ma
Division of Comparative Immunology and Oncology, Department of Pathophysiology and Allergy Research, Vienna General Hospital, Medical University of Vienna, Austria
Prostate carcinoma is one of the most commonly diagnosed types of cancer and the second most common cause of cancer mortality among men in the USA and in Europe. We aimed to define and explore new therapeutic approaches and novel biomarkers for diagnosis and treatment of prostate cancer.
The Chinese medicinal herb Panax quinquefolius has been tested for its tumor selectivity and cytotoxic efficacy. P. quinquefolius saponins (PQS) have been shown to have anti-tumor effects in vivo and in vitro. In this study human prostate cancer cells DU145 and immortalized normal prostate epithelial cells PNT2 were
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
vol. 15/2017/suppLeMEnT 1
