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gadolinium- and curcumin-loaded micelles based on a-fetoprotein functionalized amphiphilic block copolymers
Pozdniakova NV1Ea, Grigorieva EYu1, Shevelev AB2
1 Laboratory of Radionuclide and Beam Technologies in Experimental Oncology, N. N. Blokhin Russian Cancer Research Center, Moscow
2 Laboratory of Light Sensitization,
N. M. Emanuel Institute of Biochemical Physics, RAS, Moscow
This article describes a method of obtaining curcumin- and gadolinium-loaded micelles based on triblock amphiphilic polyethylene glycol and polypropylene glycol copolymers Pluronic F-127 and Pluronic P-123 (Sigma-Aldrich, USA) superficially functionalized with recombinant human a-fetoprotein. The size of nanoparticles was measured using dynamic light scattering and amounted to an average of 50 to 100 nm. The micelles were stable: stored at +4 °C for 10 days, they exhibited no changes in their properties that would not fall within the standard error of measurement for the methods used for the analysis. Preliminary in vivo experiments conducted on mice showed no conspicuous toxicity of micelles with the maximum possible concentration of gadolinium, which enables their use in tumor tissue imaging in vivo.
Keywords: imaging, contrast agents, gadolinium, curcumin, micelles, Pluronic F-127, Pluronic P-123
Funding: the study was supported by the Ministry of Education and Science of the Russian Federation (Grant Agreement no.14.607.21.0133 dated October 27, 2015, Project ID RFMEFI60715X0133).
[x] Correspondence should be addressed: Natalia Pozdniakova
Kashirskoe shosse, d. 24, Moscow, Russia, 115478; [email protected]
Received: 23.06.2016 Accepted: 27.06.2016
мицеллярные композиции на основе функционализированных а-фетопротеином амфифильных блок-сополимеров, содержащих гадолиний и куркумин
Н. В. Позднякова1 Е. Ю. Григорьева1, А. Б. Шевелев2
1 Лаборатория радионуклидных и лучевых технологий в экспериментальной онкологии, Российский онкологический научный центр имени Н. Н. Блохина, Москва
2 Лаборатория процессов фотосенсибилизации,
Институт биохимической физики имени Н. М. Эмануэля РАН, Москва
Предложен метод получения мицеллярных композиций куркумина и ионов гадолиния на основе трехблочных амфифильных сополимеров полиэтиленгликоля и полипропиленгликоля Pluronic F-127 и Pluronic P-123 (Sigma-Aldrich, США), поверхность которых функционализирована рекомбинантным производным а фетопротеина человека. Методом динамического светорассеяния определили размер наночастиц, и он составил в среднем 50-100 нм. Композиции отличались устойчивостью: в течение 10 суток хранения при +4 °С характеристики мицелл изменялись в пределах стандартной ошибки измерения для выбранных аналитических методов. Предварительные эксперименты in vivo на мышах показали отсутствие явно выраженной токсичности композиций при максимально возможной концентрации гадолиния, что делает возможным их дальнейшее использование для визуализации опухолевых тканей in vivo.
Ключевые слова: визуализация, контрастные средства, гадолиний, куркумин, мицеллярная композиция, Pluronic F-127, Pluronic P-123
Финансирование: исследование поддержано Министерством образования и науки Российской Федерации (Соглашение о предоставлении субсидии № 14.607.21.0133 от 27.10.2015, уникальный идентификатор RFMEFI60715X0133).
Ек] Для корреспонденции: Позднякова Наталья Владимировна 115478, г. Москва, Каширское шоссе, д. 24; [email protected]
Статья получена: 23.06.2016 Статья принята в печать: 27.06.2016
Due to its unique paramagnetic properties, gadolinium (Gd3+) holds a firm place in medical diagnostics (magnetic resonance imaging, MRI) and therapy (secondary thermal radiation used for eliminating tumor cells) [1]. Electron spin-lattice relaxation time T1 of gadolinium-based contrast agents is about 0.1 ns, i.e., an order of magnitude shorter than proton relaxation time in water [2]. Since Gd3+ is very toxic even at low concentrations that are routinely used for enhanced MRI, it must be chelated with agents that have a high effective association constant, such as diethylenetriaminepentaacetic acid [3]. Nanoparticles containing Gd3+ chelates are widely used in clinical practice for performing contrast-enhanced MRI of various tissues on living patients, including scans of brain structures [4], vascular plexuses [5], myocardium and coronary arteries [6].
Kang et al. [7] have described a method of preparing gadolinium oxide nanoparticles Gd@SiO2-DO3A and Gd@SiO2-DO2A-BTA of 50-60 nm in size. Nanoparticles were synthesized from tetraethyl orthosilicate (TEOS) and (3-aminopropyl) triethoxysilane (APTES); then, aminopropylsilane groups were functionalized with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) or 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid conjugates of benzothiazole (DO3A-BTA). The obtained nanoparticles were highly soluble in water; their colloid solution was stable. Their relaxivity r1 was much higher than r1 of low molecular weight contrast agents; the ratio r2/r1 [8] was close to 1, which indicates that the investigated nanoparticles can be used as MRI contrast agents [9]. Biodistribution analysis showed that Gd@SiO2-DO2A-BTA is excreted in bile and urine. The nanoparticles also accumulated in tumor cells and were found efficient for in vivo elimination of such continuous cell lines as SK-HEP-1, MDA-MB-231, HeLa and Hep 3B. Although some authors report cumulative toxic effects of nanostructured gadolinium-based contrast agents [10, 11], including renal toxicity [12], the latter remain an attractive subject for research, as they can be used in MRI and cancer therapies [13].
The aim of this work was to elaborate a technique for obtaining a Gd3+ — based low-toxic micellar formulation for visualizing tumor tissues in vivo.
METHODS
To ensure that the system is low toxic and has affinity to tumor cells and to prevent its non-specific binding to normal tissues, three solutions were suggested.
First, we decided to use curcumin as a complexing agent. Curcumin is a non-toxic natural compound (fig. 1). It is abundant in the roots of Indian saffron Curcuma longa. Some of its properties come in handy for our research [14]: it can form stable coordinate bonds with transition metals [15], it is not toxic at usual concentrations, and its hydrophobicity allows accommodating its compounds in the micelle core and ensures micelle stability in aqueous solutions.
Second, we obtained a micellar formulation of the compound. Methods of preparing micellar formulations have already been employed by biotechnology [16] and can possibly find their way into pharmaceutical industry [17]. Micellar formulations have some advantages over other types of carriers: in aqueous solutions, they self-assemble into structures with a hydrophobic core and a hydrophilic surface, which makes it possible to accommodate hydrophobic agents inside the micelle and thus protect them from inactivation in biological media. Micelles are small (<100 nm), easy to formulate and have long blood circulation time [18]. Among the advantages of block copolymer (Pluronic)-based micelles
are low toxicity, weak immunogenicity and simplicity of surface modification with functional groups necessary for obtaining certain properties. For this work, we chose amphiphilic triblock copolymers of polyethylene glycol and polypropylene glycol Pluronic F-127 and Pluronic P-123 (Sigma-Aldrich, USA) (fig. 2)
Third, we improved the technique of conferring target specificity to a micelle by immobilizing to its surface a recombinant vector protein (a fragment of human alpha-fetoprotein with high affinity to tumors of various types) [19].
The following reagents by Sigma-Aldrich, USA, were used in the study: gadolinium(III)nitrate hexahydrate, triblock copolymers Pluronic F-127 and Pluronic P-123, triethylamine, curcumin, 1,1'-carbonyldiimidazole, N-hydroxysuccinimide, 2,4,6-trinitrobenzenesulfonic acid, tetrahydrofuran, dimethyl sulfoxide, methanol, dimethylformamide, ethanolamine, cobalt thiocyanate, barium hydroxide. The protocol for obtaining human alpha-fetoprotein-based vector protein was described previously [19].
Preparation of curcumin - gadolinium complexes
Complexes were prepared in the organic solvent tetrahydrofuran by mixing gadolinium nitrate, curcumin and triethylamine at molar ratio of 3:1:1, heating the mixture to 50 °C for 30 minutes. Then, triethylamine and the solvent were removed in the rotary evaporator. Details are given below. 100 pl of 0.5 M curcumin solution in tetrahydrofuran were mixed with 300 pl of 0.5 M gadolinium nitrate solution in tetrahydrofuran. Then, 100 pl of 0.5 M tryethylamine solution in methanol were added dropwise over 1-2 minutes while stirring the mixture vigorously. The mixture was heated to 50 °C for 30 minutes while stirring. Solvents and triethylamine were removed from the solution at 50 °C using the rotary evaporator; the solids were washed in methanol and air-dried.
Qualitative assessment of Gd3+ complex formulation was performed on the Cary 50 Scan UV-Vis spectrophotometer (Agilent Technologies, USA) with a scan range set to 300650 nm, in a 1 cm-pathlength disposable cuvette (Sigma-Aldrich, item no. Z330418). Dry aliquots (weight ranging from 1 to several mg) were collected with a spatula. Mother liquor was diluted down with dimethyl sulfoxide (DMSO) to obtain the optical density of 0.5 to 1.5 at 450 nm. To determine complex concentration, the peak height was measured at 455 nm (with free curcumin the peak is absent). To find the peak height [20], we deducted spectral data obtained from free curcumin solution in DMSO from the spectral data of the reaction mix.
HO
о
Fig. 1. Chemical structure of curcumin
CH3
OH
OCH3
H
CH
O
\
CH2
CH
O
CH2
CH
\
CH
OH
Fig. 2. Structure of amphiphilic triblock copolymers Pluronic F-127 and Pluronic P-123 (Sigma-Aldrich, USA), where a represents the number of hydrophilic monomers and b represents the number of hydrophobic monomers. For Pluronic F-127, the a-b-a formula is 98-67-98. For Pluronic P-123, the a-b-a formula is 20-70-20.
CH3O
O
O
b
a
a
Preparation of a modified copolymer Pluronic F-127 with NHS end groups for vector protein immobilization to micelle surface
Pluronic F-127 was modified in dimethylformamide (DMFA) in two steps. At first, hydroxyl end groups were activated by carbonyldiimidazole (CDIz). Next, a reaction with N-hydroxysuccinimide (NHS) was carried out (fig. 3). Briefly, 204 mg of Pluronic F-127, 19 mg of NHS and 26 mg of CDlz were dry-mixed. Then, DMFA was added to bring the final volume of the reaction mixture to 500 pl. The mixture was stirred for 1 hour at 37 °C until CO2 gas bubbling ceased. Excess CDlz was removed by adding 50 pl water to the reaction mixture. After excess CDlz was hydrolyzed, the modified copolymer was extracted several times with diethyl ether. The solids were air-dried.
The number of NHS end groups in the modified Pluronic F-127 was measured by performing a reaction with excess ethanolamine in 10 mM borate buffer (pH 8.5) with subsequent titration of unreacted amino groups by 2,4,6-trinitrobenzenesulphonic acid (TNBS), as described previously [21].
Micelle formulation
Various copolymer-based micellar systems were prepared as described below. Weighted amounts of the curcumin/ gadolinium complex and modified pluronics were dissolved in tetrahydrofuran and mixed together. The amount and the ratio of the components were different in various micellar systems (see the table). Then, the solution was poured into a rotary evaporator flask, and the organic solvent was removed at 50 °C while the flask was rotating. Then, distilled water was added and the mixture was stirred vigorously for 15 minutes and centrifuged. Supernatant was removed; the mixture was dialyzed against the phosphate-buffered saline (pH 7.5) at +4 °C for 24 hours.
To assess how efficiently the investigated substance was encapsulated into the micelles (%), the following formula was applied:
M x 100
E — enc enc
Minit
where M is the mass of the encapsulated curcumin-
enc
gadolinium complex, and Minit is the initial mass of the curcumin-gadolinium complex.
Concentration load (% m/m) was calculated according to the following formula:
L
M x 100
enc
: m +m ,
enc pol
where Mpol is the copolymer mass.
Chemical modification of micelle surface
Micelles were conjugated to protein by mixing the copolymer-based micellar solution and the protein solution, in molar excess of the modified copolymer (NHS groups) to the protein. For functionalization, we used recombinant domain 3 of human alpha-fetoprotein [19]. The detailed protocol is below. 1 ml of the micellar solution was mixed with 200 pl of 0.1 mM protein solution (concentration of 3 mg/ml) in the phosphate-buffered saline (pH 8.0) and incubated for 1 hour at room temperature. To deactivate excess reactive NHS groups, excess ethanolamine was added after incubation, until its concentration in the solution was 10 mM; the mixture was then incubated at room temperature for 2 hours and dialyzed against the phosphate-buffered saline (pH 7.2) at +4 °C for 24 hours. The obtained substance was stored at +4 °C for one week.
Qualitative detection of protein in the obtained micelle composition was performed in a polyacrylamide gel by electrophoresis with subsequent staining for 10 minutes in a 5% aqueous solution of barium iodide [22].
Evaluation of micelle size
The average size of the micelles and size distribution were evaluated by dynamic light scattering on ZetasizerNano ZS (Malvern Ltd., UK) in thermostatic plastic microcuvettes ZEN0040. Measurements were performed using the heliumneon laser with a wavelength of 633 nm and a power level of 4 mW at 25 °C. Prior to measurements, samples were diluted tenfold with deionized water. Measurements for each sample were performed in replicates of 3. Mean root square deviation was calculated.
Determination of gadolinium content in micelles
Measurements were performed on the X-ray fluorescence analyzer X-art M (Komita, Russia). The signal from the studied samples was compared to the signal from the solutions of known gadolinium concentrations.
■ H —
'CH2 O "CH,
CH3
I 3
/CH^ O CH,
CH2 ■O CH2
Activation of hydroxyl end groups by carbonyldiimidazole
Determination of curcumin content in micelles
To determine curcumin content, absorption spectra of the micelles and calibration solutions of known concentration were measured in a disposable cuvette (Sigma-Aldrich, item no. Z330418) at 430 nm wavelength on the Cary 50 Scan UV-Vis spectrophotometer.
Reaction with N-hydroxysuccinimide
H —
,CH, O ~CH,
CH3
I 3 / ch
O ~CH„
CH,
"O CH,
Fig. 3. Chemical schematic of Pluronic F-127 modification
Measuring polymer dry matter content
Dry matter content was measured using a previously described method [23] with slight modifications. The reagent was prepared as follows: 0.2 ml of 2.6 M aqueous solution of cobalt thiocyanate (CoSCN) were mixed with 0.8 mL of 0.8 M barium hydroxide aqueous solution. A 10 pl aliquot of pluronic-containing solution was mixed with 10 pl of the reagent, stirred vigorously and centrifuged; supernatant was removed; the solids were air-dried. Then, 100 pl of DMSO and 5 pl of 5 M HCl
b
a
b
a
a
were added to the solids. Absorption was measured at 630 nm on the Titertek Multiscan® Plus reader (LabX, Canada) in 96-well plates. Calibration solutions of known concentration were measured in parallel. Using a calibration curve, concentration in the analyzed sample was calculated.
Determination of total protein content
Total protein content was evaluated by a modified Lowry method using bicinchoninic acid (Sigma-Aldrich). A standard bovine serum albumin solution was used as a calibration solution [24].
Assesment of toxicity of curcumin-gadolinium micelle formulation
To verify that further in vivo research is possible, we investigated animal tolerance to the obtained micelle formulation. We used 10 C57/black female mice with an average weight of 25 g. The animals were administered the micellar formulation composed of 10 mM Gd3+, 10 mM curcumin, 5 mM Pluronic F-127 and phosphate-buffered saline (pH 7.0). Micelle diameter was 20 nm. The animals received a single 200 pl injection into the tail vein. Dosage was selected based on the pluronic concentration optimal for delivering the cytostatic drug Docetaxel to lung tumor cells in vivo in the mice model [25]. The animals were observed for 2 months. Then, they were sacrificed; autopsy and histological analysis were performed subsequently.
RESULTS
The process of curcumin-gadolinium complex formation was controlled by recording spectral changes, such as the additional peak occurring at 455 nm [20] (fig. 5).
The copolymer:NHS ratio in the modified pluronic F-127 was about 1:1. In total, we obtained about 100 pl of dry modified copolymer containing one NHS group per molecule.
Encapsulation efficiency of the method applied for micelle formulation varied from 40 to 80 % and load concentration varied from 10 to 25 % m/m. As a result, we obtained a number
of micelle solutions with various amounts of basic components (see the table).
Curcumin/gadolinium ratio in the micelles implies vacant valence orbitals in a gadolinium atom that are presumably occupied by water molecules, which is essential for preserving contrasting properties during MRI scans [26]. As far as micelle stability goes, we should note that evaluation of micelle sizes by dynamic light scattering and measurements of visible absorption spectrum typical for the curcumin-gadolinium complex were repeated after the micelles had been stored at +4 °C for 10 days. All values remained unchanged. Differences fell within the standard error of measurement.
The size of the obtained micelles was approximating (but no exceeding) 100 nm. It allows micelles to circulate in the blood for a long time without being captured by the hepatic portal and reticuloendothelial systems [27]. This property is important for ensuring that micelles are delivered to tumor tissues, owing to the immobilized functional protein agent on their surface [28].
During the experiment on mice, no visible signs of toxicity of the micelle system were detected. We did not observe symptoms of heavy metal poisoning [1], such as collaptoid reactions or bleeding. The animals had a good appetite and were active; there were no signs of pathological thirst or photophobicity.
DISCUSSION
A number of works [14, 29] prove that curcumin can be used as a complexing agent that suppresses gadolinium toxicity in vivo. Patil et al. [29] used such complexes for detecting amyloid plaques in Alzheimer's patients. The researchers exploited the natural affinity of curcumin to p-amyloids. Our work is the first to demonstrate the possibility of encapsulating hydrophobic curcumin complexes into micelles formed by pluronics (block copolymers of polyethylene glycol and polypropylene glycol). Such micelles exhibit stability in aqueous media, therefore, curcumin pharmacokinetic properties no longer play a key role in distributing Gd3+ ions in body tissues, because the chelated complex remains highly stable.
Other authors [7] worked on an almost identical task, but found a different solution to it: they encapsulated Gd3+ ions
Micell formation
Intermediate compounds
Initial components
Conjugation to protein
Curcumin
Curcumin-Gd
Gd3+
F-127-NHS
P-123, F-127
F-127 modification
Fig. 4. Schematic of obtaining gadolinium-containing functionallzed micelles
0,2 -
0,0 -
400
500
—1-
600
At the same time, defective or leaky tumor vasculature allows micelles to be retained in tumor tissues [30].
Additional enhancement of target specificity of Gd3+ containing micelles can be achieved through using various target-specific functional groups. Domain 3 of a-fetoprotein employed for this work has a high affinity to receptors of various tumor types, since, like albumin, it uses them as a nutrient [19]. The suggested technique can efficiently immobilize any protein, peptide or other target-specific groups to micelle surfaces.
CONCLUSIONS
- Curcumin - [Curcumin-Gd]
Fig. 5. Spectral data of curcumin and curcumin-Gd3+ complex
into silicon. It proves that the task itself is of high practical significance. The technique we suggest has an advantage over the one described in work [7]: it can be implemented in a biochemical laboratory using commercially available reagents and standard equipment, with almost 100% yield and without recruiting professional synthetic chemists. This technique also allows both small and commercial scale production of Gd3+ containing nanoparticles. Despite its simplicity, the absolute size of particles, size distribution and the amount of the encapsulated substance do not differ from the corresponding values suggested in [8]. It became possible due to those curcumin properties that make it a natural Gd3+ chelator.
Micelles have an important advantage over hard silicon oxide nanoparticles. They can circulate in the blood for a long time without being destroyed by liver monocyte derivatives [9].
We have demonstrated that curcumin-gadolinium complexes are efficiently encapsulated and strongly retained in micelles formed by polyethylene glycol and polypropylene glycol copolymers (pluronics). Stored at +4 °C for 10 days, the micelles exhibited no changes in their properties that would not fall within the standard error of measurement. The micelles have a standard size of 50-100 nm depending on the ratio of their components and a prolonged blood circulation time.
We suggest a technique for modifying the surface of pluronic mycelles containing curcumin and Gd3+ complexes using target-specific molecules, such as domain 3 of human a-fetoprotein.
Due to its simplicity, high efficiency and a possibility to work with scant amounts of components, this method is intended primarily for studying distribution of macromolecules and their complexes in vivo. It can also be used for screening functional target-specific groups that deliver biomacromolecules and their complexes to tissues and cells of various types, including tumors.
Micelle composition
№ Components Concentration of components Mean micelle diameter, nm
1 Gd3+ - Curcumin - P-123 Gd3+ - 12 MM Curcumin - 12 MM P-123 - 3 MM 57.0 ± 1.2
2 Gd3+ - Curcumin - F-127 Gd3+ - 10 MM Curcumin - 10 MM F-127 - 5 MM 20.0 ± 0.9
3 Gd3+ - Curcumin - P-123 - F-127 Gd3+ - 9 MM Curcumin - 21 MM P-123 - 2.7 MM F-127 - 2.7 MM 83.0 ± 1.4
4 Gd3+ - Curcumin - F-127 - Protein Gd3+ - 57 MM Curcumin - 30 MM F-127 - 4 MM Protein - 0.7 Mr/urn 32.0 ± 2.2
5 Gd3+- Curcumin - P-123 - F-127 - Protein Gd3+ - 18 MM Curcumin - 8.4 MM P-123 - 2.5 MM F-127 - 2.5 MM Protein - 1.1 Mr/urn 69.0 ± 1.3
Note: P-123 — Pluronic P-123, F-127 — Pluronic F-127 (Slgma-Aldrlch, USA).
References
1. Goischke HK. MRI with gadolinium-based contrast agents: 15. practical help to ensure patient safety. J Am Coll Radiol. 2016
Jun 18. pii: S1546-1440(16)30315-5.
2. Dinger SC, Fridjhon P, Rubin DM. Thermal Excitation of Gadolinium-Based Contrast Agents Using Spin Resonance. 16 PLoS One. 2016 Jun 24; 11 (6): e0158194.
3. Saito K, Yoshimura N, Shirota N, Saguchi T, Sugimoto K., Tokuuye K. Distinguishing liver haemangiomas from metastatic tumours using gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid-enhanced diffusion-weighted imaging at 17. 1.5T MRI. J Med Imaging Radiat Oncol. 2016 Jun 21. doi: 10.1111/1754-9485.12487. [Epub ahead of print]. PubMed PMID: 27324436. 18.
4. Hu HH, Pokorney A, Towbin RB, Miller JH. Increased signal intensities in the dentate nucleus and globus pallidus on unenhanced T1-weighted images: evidence in children undergoing multiple gadolinium MRI exams. Pediatr Radiol. 2016 Jun 9. [Epub ahead of print]. PubMed PMID: 27282825.
5. Schnell S, Wu C, Ansari SA. Four-dimensional MRI flow 19. examinations in cerebral and extracerebral vessels — ready for clinical routine? Curr Opin Neurol. 2016 Aug; 29 (4): 419-28. [Epub ahead of print]. PubMed PMID: 27262148. 20.
6. Swoboda PP, McDiarmid AK, Erhayiem B, Haaf P, Kidambi A, Fent GJ, et al. A novel and practical screening tool for the detection
of silent myocardial infarction in patients with type 2 diabetes. 21. J Clin Endocrinol Metab. 2016 Jun 14. [Epub ahead of print]. PubMed PMID: 27300573. 22.
7. Kang MK, Lee GH, Jung KH, Jung JC, Kim HK, Kim YH, et al. Gadolinium Nanoparticles Conjugated with Therapeutic Bifunctional Chelate as a Potential T1 Theranostic Magnetic Resonance Imaging Agent. J Biomed Nanotechnol. 2016 May; 23 12 (5): 894-908.
8. De León-Rodríguez LM, Martins AF, Pinho MC, Rofsky NM, Sherry AD. Basic MR relaxation mechanisms and contrast agent 24. design. J Magn Reson Imaging. 2015 Sep; 42 (3): 545-65.
9. Yang CT, Chuang KH. Gd(III) chelates for MRI contrast agents:
from high relaxivity to "smart", from blood pool to blood-brain 25. barrier permeable. Medchemcomm. 2012; 3: 552-65.
10. Maximova N, Gregori M, Zennaro F, Sonzogni A, Simeone R, Zanon D. Hepatic gadolinium deposition and reversibility after contrast agent-enhanced MR imaging of pediatric hematopoietic 26. stem cell transplant recipients. Radiology. 2016 Jun 8: 152846. [Epub ahead of print]. PubMed PMID: 27276243.
11. Vorobiov M, Basok A, Tovbin D, Shnaider A, Katchko L, 27. Rogachev B. Iron-mobilizing properties of the gadolinium-DTPA complex: clinical and experimental observations. Nephrol Dial Transplant. 2003 May; 18 (5): 884-7.
12. Ersoy H, Rybicki FJ. Biochemical safety profiles of gadolinium- 28. based extracellular contrast agents and nephrogenic systemic fibrosis. J Magn Reson Imaging. 2007 Nov; 26 (5): 1190-7.
13. Le Duc G, Roux S, Paruta-Tuarez A, Dufort S, Brauer E, Marais A, 29. et al. Advantages of gadolinium based ultrasmall nanoparticles vs molecular gadolinium chelates for radiotherapy guided by MRI for glioma treatment. Cancer Nanotechnol. 2014; 5 (1): 4.
14. Heger M, van Golen RF, Broekgaarden M, Michel MC. The 30. molecular basis for the pharmacokinetics and pharmaco-dynamics of curcumin and its metabolites in relation to cancer. Pharmacol Rev. 2013 Dec 24; 66 (1): 222-307.
Pröhl M, Schubert US, Weigand W, Gottschaldt M. Metal complexes of curcumin and curcumin derivatives for molecular imaging and anticancer therapy. Coord Chem Rev. 2016 Jan 15; 307 (Pt 1): 32-41.
Carstens MG, Rijcken CJF, van Nostrum CF, Hennink WE. Pharmaceutical micelles: combining longevity, stability and stimuli sensitivity. In: Fundamental Biomedical Technologies. Vol. 4: Torchilin V, editor. Multifunctional Pharmaceutical Nanocarriers. New York: Springer; 2008. p. 263-308.
Oerlemans C, Bult W, Bos M, Storm G, Nijsen JF, Hennink WE. Polymeric micelles in anticancer therapy: targeting, imaging and triggered release. Pharm Res. 2010 Dec; 27 (12): 2569-89. Alexandridis P, Hatton TA. Polyethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) block copolymer surfactants in aqueous solutions and at interfaces: thermodynamics, structure, dynamics, and modeling. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 1995 Mar 10; 96 (12): 1-46.
Pozdniakova NV, Gorokhovets NV, Gukasova NV, Bereznikova AV, Severin ES. New protein vector ApE1 for targeted delivery of anticancer drugs. J Biomed Biotechnol. 2012; 2012: 469756. Ferrari E, Asti M, Benassi R, Pignedoli F, Saladini M. Metal binding ability of curcumin derivatives: a theoretical vs. experimental approach. Dalton Trans. 2013 Apr 21; 42 (15): 5304-13. Fields R. The rapid determination of amino groups with TNBS. Methods Enzymol. 1972; 25: 464-8.
Kurfürst MM. Detection and molecular weight determination of polyethylene glycol-modified hirudin by staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem. 1992 Feb 1; 200 (2): 244-8.
Crabb NT, Persinger HE. The determination of polyoxyethylene nonionic surfactants in water at the parts per million level. J Am Oil Chem Soc. 1964; 41 (11): 752-5.
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, et al. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct; 150 (1): 76-85. Chen L, Sha X, Jiang X, Chen Y, Ren Q, Fang X. Pluronic P105/ F127 mixed micelles for the delivery of docetaxel against Taxol-resistant non-small cell lung cancer: optimization and in vitro, in vivo evaluation. Int J Nanomedicine. 2013; 8: 73-84. Caravan P, Ellison JJ, McMurry TJ, Lauffer RB. Gadolinium(III) chelates as MRI contrast agents: structure, dynamics, and applications. Chem Rev. 1999 Sep 8; 99 (9): 2293-352. Hobbs SK, Monsky WL, Yuan F, Roberts WG, Griffith L, Torchilin VP, et al. Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment. Proc Natl Acad Sci U S A. 1998 Apr 14; 95 (8): 4607-12. Maruyama K. Intracellular targeting delivery of liposomal drugs to solid tumors based on EPR effects. Adv Drug Deliv Rev. 2011 Mar 18; 63 (3): 161-9.
Patil R, Gangalum PR, Wagner S, Portilla-Arias J, Ding H, Rekechenetskiy A, et al. Curcumin targeted, polymalic acid-based MRI contrast agent for the detection of Aß plaques in Alzheimer's disease. Macromol Biosci. 2015 Sep; 15 (9): 1212-7. Jhaveri AM, Torchilin VP. Multifunctional polymeric micelles for delivery of drugs and siRNA. Front Pharmacol. 2014 Apr 25; 5: 77.
Литература
1. Goischke HK. MRI with gadolinium-based contrast agents: practical help to ensure patient safety. J Am Coll Radiol. 2016 Jun 18. pii: S1546-1440(16)30315-5.
2. Dinger SC, Fridjhon P, Rubin DM. Thermal Excitation of Gadolinium-Based Contrast Agents Using Spin Resonance. PLoS One. 2016 Jun 24; 11 (6): e0158194. 4.
3. Saito K, Yoshimura N, Shirota N, Saguchi T, Sugimoto K., Tokuuye K. Distinguishing liver haemangiomas from metastatic
tumours using gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid-enhanced diffusion-weighted imaging at 1.5T MRI. J Med Imaging Radiat Oncol. 2016 Jun 21. doi: 10.1111/1754-9485.12487. [Epub ahead of print]. PubMed PMID: 27324436.
Hu HH, Pokorney A, Towbin RB, Miller JH. Increased signal intensities in the dentate nucleus and globus pallidus on unenhanced T1-weighted images: evidence in children
undergoing multiple gadolinium MRI exams. Pediatr Radiol. 2016 Jun 9. [Epub ahead of print]. PubMed PMID: 27282825.
5. Schnell S, Wu C, Ansari SA. Four-dimensional MRI flow 17. examinations in cerebral and extracerebral vessels — ready for clinical routine? Curr Opin Neurol. 2016 Aug; 29 (4): 419-28. [Epub ahead of print]. PubMed PMID: 27262148. 18.
6. Swoboda PP, McDiarmid AK, Erhayiem B, Haaf P, Kidambi A, Fent GJ, et al. A novel and practical screening tool for the detection of silent myocardial infarction in patients with type 2 diabetes. J Clin Endocrinol Metab. 2016 Jun 14. [Epub ahead of print]. PubMed PMID: 27300573.
7. Kang MK, Lee GH, Jung KH, Jung JC, Kim HK, Kim YH, 19. et al. Gadolinium Nanoparticles Conjugated with Therapeutic Bifunctional Chelate as a Potential T1 Theranostic Magnetic Resonance Imaging Agent. J Biomed Nanotechnol. 2016 May; 20. 12 (5): 894-908.
8. De León-Rodríguez LM, Martins AF, Pinho MC, Rofsky NM, Sherry AD. Basic MR relaxation mechanisms and contrast agent 21. design. J Magn Reson Imaging. 2015 Sep; 42 (3): 545-65.
9. Yang CT, Chuang KH. Gd(III) chelates for MRI contrast agents: 22. from high relaxivity to "smart", from blood pool to blood-brain barrier permeable. Medchemcomm. 2012; 3: 552-65.
10. Maximova N, Gregori M, Zennaro F, Sonzogni A, Simeone R, Zanon D. Hepatic gadolinium deposition and reversibility after 23. contrast agent-enhanced MR imaging of pediatric hematopoietic
stem cell transplant recipients. Radiology. 2016 Jun 8: 152846. [Epub ahead of print]. PubMed PMID: 27276243. 24.
11. Vorobiov M, Basok A, Tovbin D, Shnaider A, Katchko L, Rogachev B. Iron-mobilizing properties of the gadolinium-DTPA complex: clinical and experimental observations. Nephrol Dial 25. Transplant. 2003 May; 18 (5): 884-7.
12. Ersoy H, Rybicki FJ. Biochemical safety profiles of gadolinium-based extracellular contrast agents and nephrogenic systemic fibrosis. J Magn Reson Imaging. 2007 Nov; 26 (5): 1190-7. 26.
13. Le Duc G, Roux S, Paruta-Tuarez A, Dufort S, Brauer E, Marais A, et al. Advantages of gadolinium based ultrasmall nanoparticles vs molecular gadolinium chelates for radiotherapy guided by MRI for 27. glioma treatment. Cancer Nanotechnol. 2014; 5 (1): 4.
14. Heger M, van Golen RF, Broekgaarden M, Michel MC. The molecular basis for the pharmacokinetics and pharmaco-dynamics of curcumin and its metabolites in relation to cancer. 28. Pharmacol Rev. 2013 Dec 24; 66 (1): 222-307.
15. Pröhl M, Schubert US, Weigand W, Gottschaldt M. Metal complexes of curcumin and curcumin derivatives for molecular 29. imaging and anticancer therapy. Coord Chem Rev. 2016 Jan 15;
307 (Pt 1): 32-41.
16. Carstens MG, Rijcken CJF, van Nostrum CF, Hennink WE. Pharmaceutical micelles: combining longevity, stability and stimuli 30. sensitivity. In: Fundamental Biomedical Technologies. Vol. 4:
Torchilin V, editor. Multifunctional Pharmaceutical Nanocarriers. New York: Springer; 2008. p. 263-308.
Oerlemans C, Bult W, Bos M, Storm G, Nijsen JF, Hennink WE. Polymeric micelles in anticancer therapy: targeting, imaging and triggered release. Pharm Res. 2010 Dec; 27 (12): 2569-89. Alexandridis P, Hatton TA. Polyethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) block copolymer surfactants in aqueous solutions and at interfaces: thermodynamics, structure, dynamics, and modeling. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 1995 Mar 10; 96 (12): 1-46.
Pozdniakova NV, Gorokhovets NV, Gukasova NV, Bereznikova AV, Severin ES. New protein vector ApE1 for targeted delivery of anticancer drugs. J Biomed Biotechnol. 2012; 2012: 469756. Ferrari E, Asti M, Benassi R, Pignedoli F, Saladini M. Metal binding ability of curcumin derivatives: a theoretical vs. experimental approach. Dalton Trans. 2013 Apr 21; 42 (15): 5304-13. Fields R. The rapid determination of amino groups with TNBS. Methods Enzymol. 1972; 25: 464-8.
Kurfürst MM. Detection and molecular weight determination of polyethylene glycol-modified hirudin by staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem. 1992 Feb 1; 200 (2): 244-8.
Crabb NT, Persinger HE. The determination of polyoxyethylene nonionic surfactants in water at the parts per million level. J Am Oil Chem Soc. 1964; 41 (11): 752-5.
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, et al. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct; 150 (1): 76-85. Chen L, Sha X, Jiang X, Chen Y, Ren Q, Fang X. Pluronic P105/ F127 mixed micelles for the delivery of docetaxel against Taxol-resistant non-small cell lung cancer: optimization and in vitro, in vivo evaluation. Int J Nanomedicine. 2013; 8: 73-84. Caravan P, Ellison JJ, McMurry TJ, Lauffer RB. Gadolinium(III) chelates as MRI contrast agents: structure, dynamics, and applications. Chem Rev. 1999 Sep 8; 99 (9): 2293-352. Hobbs SK, Monsky WL, Yuan F, Roberts WG, Griffith L, Torchilin VP, et al. Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment. Proc Natl Acad Sci U S A. 1998 Apr 14; 95 (8): 4607-12. Maruyama K. Intracellular targeting delivery of liposomal drugs to solid tumors based on EPR effects. Adv Drug Deliv Rev. 2011 Mar 18; 63 (3): 161-9.
Patil R, Gangalum PR, Wagner S, Portilla-Arias J, Ding H, Rekechenetskiy A, et al. Curcumin targeted, polymalic acid-based MRI contrast agent for the detection of Aß plaques in Alzheimer's disease. Macromol Biosci. 2015 Sep; 15 (9): 1212-7. Jhaveri AM, Torchilin VP. Multifunctional polymeric micelles for delivery of drugs and siRNA. Front Pharmacol. 2014 Apr 25; 5: 77.
