CC BY
15
UDC 576.89:597 (261.2)
FLORISTIC DIVERSITY OF MACROPHYTES IN KAZACHYA BAY (THE CRIMEA, THE BLACK SEA)
Nataliya Afanasiyevna Mylchakova, Valentina Gennadiyevna Ryabogina
SBE "Institute of the South Marine Biology named after Kovalevsky A.O.", 2, Nakhymova av., the city of Sevastopol, RF, 299011 milchakova@gmail.ru
Introduction
Study of macrophyte flora in Kazachya Bay has taken almost forty years, though species composition of macroalgae and higher plants inhabited its territory, including water area, adjacent to the state wildlife preserve, has been published in recent years [1 - 4, 8 - 10]. This work presents an annotated list of macrophyte flora in Kazachya bay, based on inspection of published information and hydrobotanic surveys (1997 - 2007), allowing for nomenclatural changes. A comparative ecological and floristic analysis of the long-term changes in macrophyte flora has been made within the whole bay water area [1]. The research objective is to reveal cozoological importance of macrophyte flora in Kaachya bay and to ground increasing the wildlife preserve territory.
Results and discussion
105 species present macrophyte flora of Kazachya bay: Chara- 1, Ulva latissima - 28, Porphyra gen. - 50, Phaeophyceae - 20, flowering plants - 6 (Table).
Ulva latissima group is marked out by Bryopsis hypnoides, B. plumosa, Chaetomorpha crassa, Cladophora liniformis, Cladophoropsis membranacea, Ulva intestinalis, developed everywhere and not found out before.
Table
Macrophyte s pecies composition of Kazachya bay (1967-2007)
Taxon Type of vegetation Period
1967-1987 1997-2007
1 2 3 4
Charophyta
Chara aculeolata F.T. Kütz. in H. Reichenbach A* + +
Chlorophyta
Acrochaete viridis (Reinke) R. Nielsen (= Entocladia viridis Reinke) A + +
Acrosiphonia arcta (Dillw.) Gain (= A. centralis (Lyngb.) Kjellm.) Sw - +
Bolbocoleon piliferum Pringsh. A + -
Bryopsis corymbosa J. Ag. Ss - +
B. hypnoides Lamour. A - +
B. plumosa (Huds.) C. Ag. Sw - +
Chaetomorpha aerea (Dillw.) Kütz. A + +
Ch. crassa (C. Ag.) Kütz. A - +
Ch. linum (O.F. Müller) Kütz. (= Ch. chlorotica (Mont.) Kütz.) A + +
Chlorochytrium cohnii E.P. Wright (= Chlorocystis cohnii (E.P. Wright) L. Reinhard) ? + -
Cladophora albida (Nees) Kütz. A + +
Cl. dalmatica Kütz. A + -
Cl. laetevirens (Dillw. ) Kütz. A + +
Cl. liniformis Kttz. A - +
Cl. sericea (Huds.) Kütz A + +
Cl. siwaschensis C. Meyer A - +
Cl. vadorum (Aresch.) Kütz. A + +
Cladophoropsis membranacea (Hofm. Bang ex C. Ag.) B0rg. Ss - +
Codium vermilara (Olivi) Delle Chiaje P - +
Pedobesia simplex (Menegh. ex Kütz) M.J. Wynne & Leliaert (= Derbesia lamourouxii (J. Ag.) Soland.) Ss + -
Phaeophila dendroides (P.L. Crouan & H.M. Crouan) Batt. A + -
Pringsheimiella scutata (Reinke) Marschew. A + +
Rhizoclonium riparium (Roth) Harv. (= Rh. implexum (Dillw.) Kütz.) A - +
Ulothrix implexa (Kütz.) Kütz A - +
Ulva clathrata (Roth) C. Ag. (= Enteromorpha clathrata (Roth) Gtev.) A + +
U. intestinalis L. (= Enteromorpha intestinalis (L.) Link.) A - +
U. rigida C. Ag. P + +
Ulvella lens P.L. Crouan & H.M. Crouan A - +
Rhodophyta
Acrochaetium secundatum (Lyngb.) Näg. (= Kylinia virgatula (Harv.) Papenf.) A + +
Antithamnion cruciatum (C. Ag.) Näg. A + +
Apoglossum ruscifolium (Turn.) J. Ag. P + +
Callithamnion corymbosum (J.E. Smith.) Lyngb. A + +
Ceramium arborescens J. Ag. A - +
C. ciliatum (Ell.) Ducl. Ss + +
C. deslongchampsii Chauvin exDuby (= Ceramium strictum (Kütz.) Rabenh.) A + +
C. diaphanum (Lightf.) Roth A - +
var. elegans (Roth) Roth (= Ceramium elegans (Roth) Ducl.) Ss - +
var. tenuissimum Roth (= Ceramium tenuissimum (Roth) Aresch.) A - +
C. virgatum Roth (= C. rubrum (Huds.) Ag.) A + +
C. pedicellatum C. Ag. A - +
Chondria capillaris (Huds.) M.J. Wynne (= Ch. tenuissima C. Ag.) A + +
Ch. dasyphylla (Wood.) C. Ag. A - +
Chroodactylon ornatum (C. Ag.) Basson (= Asterocytis ramosa (Thwaites) Gobi ex F. Schmitz) Ss - +
Colaconema daviesii (Dillw.) Stegenga (= Acrochaetium davesii (Dillw.) Näg.) A - +
Corallina elongata J. Ellis & Soland. (= C. mediterranea Aresch.) A + +
C. officinalis L. P - +
Dasya baillouviana (Gmel.) Mont. (= D. elegans (G. Martens) C. Ag.) Ss + -
D. hutchinsiae Harv. (= D. arbuscula Harv.) Ss - +
D. pedicellata (C. Ag.) C. Ag. Ss - +
Erythrodermis traillii (Holmes ex Batters) Guiry & Garbary (= Phyllophora traillii Holmes ex Batters) P + +
Erythrotrichia carnea (Dillw.) J. Ag. Ss + +
Eupogodon apiculatus (C. Ag.) P.C. Silva (= Dasyopsis apiculata (C. Ag.) A. Zin.) P + +
Gelidium crinale (Hare ex Turner) Gaillon P + +
G. spinosum (S.G. Gmel.) P.C. Silva, Basson & Moe P + +
(= G. latifolium Bornet ex Hauck)
Gracilaria dura (C. Ag.) J. Ag. P - +
G. gracilis (Stackh.) M. Steentoft, L.M. Irvine & W.F. Farnham (= G. verrucosa (Huds.) Papenf.) P + +
Haliptilon virgatum (Zanard.) Garbary & H.W. Johansen (= Corallina granifera Ell. et Soland.) P - +
Hydrolithon farinosum (J.V. Lamour.) D. Penrose & Y.M. Chamberlain (= Fosliellafarinosa (J.V. Lamour.) M.A. Howe;Melobesiafarinosa J.V. Lamour.) A + +
Jania rubens (L.) Lamour. P + +
Laurencia coronopus J. Ag. P + +
L. obtusa (Huds.) Lamour. P + +
Lomentaria clavellosa (Turn.) Gail. A + +
Nitophyllumpunctatum (Stackh.) Grev. P + +
Osmundea hybrida (A.P. de Candolle) K.W. Nam (= Laurencia hybrida (A.P. de Candolle) T. Lestiboudois) P + -
O.pinnatifida (Huds.) Stackh. (= Laurencia pinnatifida (Huds.) Lamour.) P + +
Palisadaperforata (Bory de Saint-Vincent) K.W. Nam (= Laurenciapapillosa (Forsk.) Grev.; Chondrophycus papillosus (C. Ag.) Garbary et Harper) P + +
Phyllophora crispa (Huds.) P.S. Dixon (= Ph. nervosa (DC.) Grev.) P + +
Phymatolithon lenormandii (J.E. Aresch.) W.H. Adey (= Lithothamnion lenormandii (J.E. Aresch.) Foslie) P + +
Pneophyllum confervicola (Kütz.) Y.M. Chamberlain (= Melobesia minutula Foslie) A - +
Pneophyllum fragile Kttz. (= Melobesia lejolisii Rosan.) A - +
Polysiphonia breviarticulata (C. Ag.) Zanard. Ss - +* *
P. denudata (Dillw.) Grev. ex Harv. A + -
P. elongata (Huds.) Spreng. P + +
P. fucoides (Huds.) Grev. (= P. nigrescens (Huds.) Grev., P. violacea (Roth) Spreng.) A - +
P. opaca (C. Ag.) Moris et De Not. P - +* *
P. pulvinata (Roth) Spreng. Ss - +* *
P. subulifera (C. Ag.) Harv. A + +
Rubrointrusa membranacea (Magnus) S.L. Clayden & G.W. Saunders (= Audouinella membranacea (Magn.) Papenf.) P + -
Spermothamnion strictum (C. Ag.) Ardiss. P + +
Stylonema alsidii (Zanard.) K.M. Drew (= Goniotrichum elegans (Chauv.) Zanard.) P - +
Ochrophyta
Cladostephus spongiosus (Huds.) C. Ag. P + +
Corynophlaea umbellata (C. Ag.) Kütz. Ss + +
Cystoseira barbata (Stackh.) C. Ag.
var. barbata P + +
f. repens A.D. Zinova & Kalugina P + +
C. crinita Duby P + +
Dictyota dichotoma (Huds.) Lamour. Ss - +
D. linearis (C. Ag.) Grev. Ss + -
D. fasciola (Roth) J.V. Lamour. (= Dilophus fasciola (Roth) Howe) Ss + +
D. spiralis Mont. (= Dilophus spiralis (Mont.) Hamel) Ss + +
Ectocarpus siliculosus (Dillw.) Lyngb. (= E. confervoides Le Jolis) Sw + +
E. fasciculatus Harv. Sw + -
Eudesme virescens (Carm. ex Berkeley) J. Ag. A + +
Feldmannia irregularis (Kütz.) G. Hamel Ss - +* *
Giraudia sphacelaroides Derb. et Sol. A + -
Myriactula rivulariae (Suhr) Feldm. Ss + +
Myriotrichia repens Hauck A - +
Nereiafiliformis (J. Ag.) Zanard. P + +
Padinapavonica (L.) Thivy in W.R. Taylor Ss + +
Scytosiphon lomentaria (Lyngb.) Link (= S. simplicissimus (Clemente) Cremades) Sw +* * +
Sphacelaria cirrosa (Roth) C. Ag. P + +
Stilophora tenella (Esper) P.C.Silva in P.C. Silva, Basson & Moe (= S. rhizodes (Ehrh.) J. Ag.) Ss + +
Zanardinia typus (Nardo) P.C. Silva in W. Greuter (= Z. prototypus Nardo) P + +
Angiospermae
Ruppia maritima L. P + +
Ruppia cirrosa (Petagna) Grande (= R. spiralis L.) P + +
Stuckenia pectinata (L.) Börner (= Potamogeton pectinatus L.) P + +
Zannichelliapalustris L. (= Z. major (Hartman) Boenn. ex Reichenb.) P + +
Zostera marina L. P + +
Zostera noltii Hornem. P + +
Всего: 105 73 97
Notes: * - P - perennial, A - annual, Sw - seasonal winter, Ss - seasonal summer; ** - data of other scientists [2 - 4]
Species diversity of Ceramium and Polysiphonia genus and some coralline red algae has been increased in comparison with last century 60-70th [5, 6]. Besides floristic changes, a number of species with short vegetation cycle has increased (Fig.) especially it concerns seasonal summer and annual macroalgae (from 16,9 up to 20,2% and from 40 up to 42,7%). At the same time perennial species decreased their contribution in the ecosystem (from 38,5 down to 32,6%) causing deterioration of ecological conditions.
Fig. Long-term changes in contribution ratio of species with different vegetation type within algal
flora, Kazachya bay (1967 - 2007)
Algal flora contains 16 rare and protected in the Black Sea species [7, 11, 12] as follows: 4 species of Ulva latissima (Cladophora dalmatica, Cl. vadorum, Cladophoropsis membranacea, Codium vermilara), 7 Porphyra gen. (Chroodactylon ornatum, Eupogodon apiculatus, Laurencia coronopus, Osmundea hybrida, O. pinnatifida, Phyllophora crispa, Stylonema alsidii) and 5 Phaeophyceae (Cladostephus spongiosus, Cystoseira barbata, C. crinita, Dictyota dichotoma, Stilophora tenella). Moreover Zostera marina and Z. Noltii are protected and included into the Black Sea Red Data Book [12].
Therefore 17,1% of macrophytes in Kazachya bay are protected species that emphasises originality of this water body and its nature conservation significance. Results of ecological and floristic analysis have been included into scientific basis concerning wildlife preservation territory increasing due to bay water area and its status change into general biological [1]. Actual task is to develop effective natural conservation measures aimed at maintenance of the unique flora within the wildlife preserve, a centre of biological organization "Gerakleisky" belonged to the Crimean maritime ecological system [9].
Conclusions
1. 105 species present macrophyte flora of Kazachya bay: Chara- 1, Ulva latissima -28, Porphyra gen. - 50, Phaeophyceae - 20, higher plants - 6.
2. 18 macrophyte species of the Black Sea basin are protected (17,1%), one of them, Zostera marina was included into Berne Convention (1979).
3. In recent decades in algaflora of Kazachya bay a number of species with short vegetation cycle has increased especially it concerns seasonal summer and annual macroalgae (from 16,9 up to 20,2% and from 40 up to 42,7%). At the same time perennial species decreased their percentage in the ecosystem (from 38,5 down to 32,6%) causing deterioration of ecological conditions.
4. Taking into consideration importance of Kazachya bay flora, it was recommended to change wildlife preserve status and make it general biological, increase its territory due to water area and work out a management-plan for development of the protected object.
Gratitude
Researches have been funded due to financial support within the 7th Framework Programme of the European Union (FP7/2007 - 2013), project COCONET «Towards COast to COast NETworks of marine protected areas (from the shore to the high and deep sea), coupled with sea-based wind energy potential» (No. 287844).
References
1. Bondaryova L.V., Mylchakova N.A. Flora obshchezoologicheskogo zakaznika "Bukhta Kazachya" (Krym, Chyornoye more) // Zapovidna sprava v Ukraini. - 2002. - T. 8, vyp.2. - S. 36 - 47.
2. Yevstigneyeva I.K. Struktura tsytozirovo- i zosterovo-laurentsiyevyh phytotsenozov v nekotoryh rayonah krymskogo poberezhya Chyornogo morya // Ekologiya morya. - 1983. - Vyp.12. - S. 35 - 41.
3. Yevstigneyeva I.K., Tankovskaya I,N. Zelyoniye vodorosli probrezhya zakaznika "Bukhta Kazachya" (Chyornoye morye) // Ryb. Hoz-vo Ukraini. - 2005. -Spetc.vypusk. - S. 85 - 89.
4. Yevstigneyeva I.K., Tankovskaya I,N. Ekologo-taksonomichesky sostav i biomassa Rhodophyta i Phaeophyta zakaznika "Bukhta Kazachya" // Ekologiya morya. -2006. - vyp. 72. - S. 21 - 29.
5. Kalugyna-Gutnyk A.A. Izmeneniya v donnoi rastitelnosti Sevastopolskoi bukhty za period s 1967 po 1977g. // Ekologiya morya. - 1982. - Vyp.9. - S. 48 - 62.
6. Kalugyna-Gutnyk A.A., Kuftarkova Ye.A., Myronova N.V. Usloviya proizrastaniya Gracilaria verrucosa (Huds.) Papenf i zapasy makrophytov v Bukhte Kazachya (Chyornoye morye) // Rastitelniye resursy. 1987. - T. 23, vyp.4. - S. 520 - 531.
7. Krasnaya kniga Rossiskoj Federatsii (rasteniya i grybi) / Gl. Redkol.: Yu.P. Trutnyev i dr.; Otv.red. R.V. Kamelyn, V.S. Novykov; Sost. T.V. Abramova i dr. - M.: Tovaryshchestvo nauchnyh izdanij KMK, 2008. - 885 s.
8. Mylchakova N.A. Makrophytobentos // sovremennoye sostoyaniye bioraznoobraziya pribrezhnyh vod Kryma (chernomorsky sector) / pod red. V.N. Yeremeyeva, A.V. Gayevskoj, NAN Ukraini, Institut biologii yuzhnyh morei. - Sevastopol: EKOSI-Gydrophysika, 2003. - S. 152 - 208.
9. Mylchakova NA., Maslov I.I., Boltachova N.A. Morskiye ohranyayemiye akvatorii v strukture ekoseti Kryma // Zapovedniki Kryma. Teoriya, praktika i perspektivi zapovednogo dela v Chernomorskom regione: materialy V mezhdunar. nauchn-prakt.konf (Simferopol, 22-23 oktyabrya 2009). - Simferopol, 2009. - S. 98 - 102.
10. Mylchakova N.A., Ryabogina V.G. Floristicheskaya kharakteristika morskih akvatorij objektov pryrodno-zapovednogo fonda regiona Sevastopola (Chyornoye morye) // Ekologiya morya. - 2002. - Vyp. 60. - S. 5 - 11.
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The article was received at editors 17.11.2014
Milchakova N.A., Ryabogina V.G. Floristic diversity of macrophytes in Kazachya bay (the Crimea, the Black Sea) // Bull. of the State Nikit. Botan. Gard. - 2015. - № 114. - P. 16-22
The article presents an annotated list of macrophytes in Kazachya bay water area, which is going to be included into the state general zoological wildlife reserve "Buhta Kazacha". Species composition of macroalgae and higher plants corresponds to archives (1967 - 1980), published information and results of hydrobotanical surveys (1997-2007), according to actual taxonomic inspection and nomenclatural changes. It was pointed a cozoological importance of macrophyte flora in Kazacha bay. This work contains a checklist of rare macroalgae and discussion relative to increasing the reserve territory due to adjacent water area.
Key words: macrophytes, species composition, long-term dynamics, rare species, wildlife preserve, Kazachya bay, the Black Sea.
PLANT BIO CHEMISTR Y
UDC 582.477:577.19
BIOLOGICALLY ACTIVE SUBSTANCES OF CUPRESSUS TORULOSA D. DON
Nadezhda Yurjevna Marchuk, Anfisa Yevgenjevna Paliy
Nikitsky Botanical Gardens - National Scientific Centre 298648, Republic of the Crimea, Yalta, urban village Nikita marchuk n@i.ua
Introduction
Essential oil is a complicated mixture of terpenoids and other components, isolated out of different parts of a plant; essential oils are used extensively in food, aromatic and pharmaceutical industries. As synthetic chemical substances are quite dangerous for health, usage of natural oils is getting popular that causes further flora investigation [10].
Bhutan Cypress (Cupressus torulosa D. Don, sin. Cupressus tonkinensis Silba, Himalayan Cypress) is an evergreen tree by height 40 m [8]. Preferable soil is limestone. It is a frost-resistant plant. For the Crimea this species was introduced by seeds from Hamburg in Nikitsky Botanical Gardens (NBG) in 1842. In the end of the XX century it was introduced on the Black sea Coast of the Caucasus. It belongs to category of park trees that's why it's mostly spread in the regions with sufficient amount of precipitation: south of France, Portuguese, Spain, north of Italy [3].
The objective of our researches was to study biologically active substances being contained in Cupressus torulosa D. Don for further usage in medicine. With this purpose a dynamic of total content of essential oils, phenol compounds and ascorbic acid in needles and cones during annual vegetation cycle was investigated, as well as component composition of essential oil having a maximum content of substances mentioned-above.
Objects and methods of the research
The researche was carried out at the laboratory of Biochemistry, biotechnology and virology of plants in NBG-NSC in 2013. Needles and cones of Cupressus torulosa D. Don were chosen as an analyzing material, collected within Arboretum of NBG-NSC.
Determination of an essential oil mass concentration was conducted by its distillation with water vapor out of a row material and further measurement of volume. Content of oil was expressed in volume weight percentage in terms of absolutely dry material [2]. The essential oil composition was determined by chromatograph Agilent Technology 6890 with mass spectrometric sensor 5973. Water heater HP-1 by length 30 m, inside diameter is 0,25 mm. Thermostat temperature was coded in range of 50°C - 250 0C with velocity 40C /min. Injector
