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14 • "PROTIST—2016
phototrophic eukaryotes. Other algae show partial (cryptophyte) or total (dinoflagellates) reduction of the redundant mitochondrially-cytosolic pathway. In dinotoms, two redundant plastid located pathways are present suggesting a presence of the two plastids of different origins in the dinotom cell. Although L. chlorophorum and B. natans contain chlorophyte-derived plastids, enzymes involved in tetrapyrrole biosynthesis are dominantly of rhodophyte origins. This may suggest an early acquisition of the rhodophyte-derived secondary plastid through the secondary endosymbiotic event on the root of SAR group.
MICROBIAL EUKARYOTES FROM THE DEEP: REVEALING THE DIVERSITY OF PROTISTS AND THEIR TROPHIC RELATIONSHIPS AT HYDROTHERMAL VENTS Countway P.D.1, Pasulka A.L.2, Mesrop L.Y.3, Hu S.K.3, Heidelberg K.B.3, Coyne K.J.4, Cary S.C.5, Caron D.A.3
1 - Bigelow Laboratory for Ocean Sciences
2 - California Institute of Technology
3 - University ofSouthern California
4 - University of Delaware
5 - University of Waikato pcountway@bigelow.org
Protists in the deep-sea are a diverse mixture of taxa that are either transported there by sinking or occur there as endemic species. It is not uncommon for the closest genetic relatives of deep-sea protists to be found in samples from other deep-sea habitats thousands of kilometers away. In other cases, the nearest relatives of deep-sea protists are from equally distant, but shallow-water habitats with similar characteristics to the deep-sea locales (e.g., low-oxygen or high-temperature sediments). Many of the most conspicuous protists at hydrothermal vents are ciliates and small flagellates that rely on the copious bacterial production occurring in the proximity of vent ecosystems. We investigated the diversity and activity of marine protists from two deep-sea hydrothermal vent ecosystems including those at Guaymas Basin (Gulf of California, N. Pacific) and 9-North (Eastern Tropical N. Pacific). Complementary molecular techniques were employed to reveal the full breadth of protistan diversity, the diversity of the active fraction, and the taxono-mic identities of some of the most conspicuous and active deep-sea ciliates. Clone libraries based on RNA extractions revealed a substantially greater proportion of the protists that appeared to be the most active via microscopy, while libraries based on DNA captured the diversity of both endemic organisms and those that sank into the vent eco-
system. Single ciliates were hand-isolated and subjected to whole genome amplification, prior to cloning and sequencing. This approach identified specific protistan taxa that were likely some of the most active members of the deep-sea community of microbial eukaryotes.
FIRST RECORDS OF MOSS CILIATES IN MEXICO
Cruz Jimenez G.1, Mayén-Estrada R.2, González Adame G.1
1 - Lab. de Ecología, Instituto de Análisis Ambiental, Universidad de la Sierra Juárez.. Av. Universidad, Ixtlán de Juárez,, C. P. 68725, Oaxaca, México
2 - Lab. Protozoología, Depto. Biol. Comparada, Fac. Ciencias, Universidad Nacional Autynoma de México, Circuito Ext. s/núm. Ciudad Universitaria, C. P. 04510, Ciudad de México, Mexico cruzgera16@hotmail.com
Mosses (Bryophyta) are not vascular gregarious plants that require some environmental characteristics to grow, i. e. the presence of some moisture degree, doing possible the establishment of ciliate communities like those that ocurre in peatlands. Mexico has a diversity of 984 species of mosses and for ciliates about 959 species have been recorded in several habitats, however the study of ciliates associated to these plants remains unknown until the present. The aim of this work was to record the ciliate community in Campylopus pilifer Brid during the summer of the year 2015 in the Sierra Juárez, Oaxaca. Samples were manually collected and observed alive using bright field and DIC microscopy. We also employed silver impregnation techniques for species identification. We recorded five species of ciliates: Colpoda inflata, C. aspera, Colpoda sp., Vorticella infusionum and Vorticella sp. in C. pilifer belonging to habitats with different environmental characteristics. These findings represent the first moss-ciliate record for Mexico. Keywords: Ciliates, Bryophytes, México, Moss, Oaxaca.
Acknowledgements. To Biyl. M. Reyes and M. C. Maricela Vicencio, Facultad de Ciencias, UNAM, for their support with technical procedures.
POPULATION STRUCTURE OF PLANKTONIC MICRO-ORGANISMS (TINTINNID CILIATES OF THE MICROZOOPLANKTON) IN THE SOUTH PACIFIC OCEAN: COMPARISON OF A HIGH PRODUCTIVITY BLOOM AND A TYPICAL OLIGOTROPHIC SITE Dolan J.R.
CNRS Laboratoire d'Océanographie de Ville-franche-sur-Mer
