CC BY
8B-I-13
FDISCO: advanced solvent-based clearing method for imaging whole organs
D. Zhu1
1Huazhong University of Science and Technology,
Wuhan National Laboratory for Optoelectronics, Wuhan, China
Various optical clearing methods have emerged as powerful tools for deep biological imaging. Organic solvent-based clearing methods, such as three-dimensional imaging of solvent-cleared organs (3DISCO), present the advantages of high clearing efficiency and size reduction for panopticimaging of large samplessuch as whole organs, and even whole bodies. However, 3DISCO results in a rapid quenching of endogenous fluorescence, which has impeded its application. Here, we propose an advanced method named FDISCO to overcome this limitation. FDISCO can effectively preserve the fluorescence of various fluorescent probes, and can achieve a long storage time of months while retaining potent clearing capability. We used FDISCO for high-resolution imaging and reconstruction of neuronal and vascular networks. Moreover, FDISCO is compatible with labelling by multiple viruses and enables fine visualization of neurons with weak fluorescence labelling in the whole brain. FDISCO represents an effective alternative to the three-dimensional mapping of whole organs and can be extensively used in biomedical studies.
References
[1] Yisong Qi,fTingting Yu,f Jianyi Xu, Peng Wan, Yilin Ma, Jingtan Zhu, Yusha Li, Hui Gong, Qingming Luo, Dan Zhu*, FDISCO:advanced solvent-based clearing methodfor imaging whole organs, Science Advances, 5(1), eaau8355, 2019.
