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About authors:
Rakitina Elena Nikolaevna, postgraduate student;
tel.: +79187618224; e-mail: rakitina_1989@bk.ru; https://orcid.org/0000-0003-0150-2662
Golubeva Marina Viktorovna, DMSc, MD, Professor, Head of the Department of children's infectious diseases; tel.: +78652264312; e-mail: mmvg@rambler.ru; https://orcid.org/0000-0002-0225-3672
Baturin Vladimir Aleksandrovich, MD, PhD, Professor, Head of the Department of clinical pharmacology with course of postgraduate and additional training; tel.: +79054901856; e-mail: prof.baturin@gmail.com; https://orcid.org/0000-0002-6892-3552
Minaev Sergey Viktorovich, MD, PhD, Professor, Head of the Department of pediatric surgery; tel.: +79624507653; e-mail: sminaev@yandex.ru; https://orcid.org/0000-0002-8405-6022
Musaelyan Olga Araratovna, postgraduate student; tel.: +79283060966; e-mail: olga.stv@mail.ru; https://orcid.org/0000-0003-3509-3481 Bolloeva Zalina Vladimirovna, Assistant of the Department;
tel.: +79187016996; e-mail: kaspolat777@yandex.ru; https://orcid.org/0000-0002-6349-1252 Borisova Yuliya Vladimirovna, postgraduate student;
tel.: +79624922330; e-mail: borisova.ula@gmail.com; https://orcid.org/0000-0002-0013-7723 Shaposhnikov Boris Sergeevich, anesthesiologist-resuscitator, postgraduate student of the Department
of children's infectious diseases; tel.: +89993793893; e-mail: mackisaew@yandex.ru; https://orcid.org/0000-0002-3333-2058
© Group of authors, 2022 UDC 620.3:579.84/86
DOI - https://doi.org/10.14300/mnnc.2022.17050 ISSN - 2073-8137
EXPERIMENTAL STUDY OF THE BIOCIDAL EFFECT OF NANOSILVER
Tofan Yu. V., Pavlova N. V., Demyanenko S. A., Kharchenko V. Z., Morozova M. N. Medical Academy name after S. I. Georgievsky
of V. I. Vernadsky Crimean Federal University, Simferopol, Russian Federation
ЭКСПЕРИМЕНТАЛЬНОЕ ИССЛЕДОВАНИЕ БИОЦИДНОГО ДЕЙСТВИЯ НАНОСЕРЕБРА
Ю. В. Тофан, Н. В. Павлова, С. А. Демьяненко, В. З. Харченко, М. Н. Морозова
Медицинская академия им. С. И. Георгиевского Крымского федерального университета им. В. И. Вернадского, Симферополь, Российская Федерация
The study demonstrated the effectiveness of nanosilver (linear particle size 10-20 nm) in the form of a patented composition. This form showed high bactericidal and fungicidal activity against gram-positive, gram-negative bacteria and candida in minimal concentrations.
Keywords: silver nanoparticles, bacteria, fungi
В исследовании продемонстрирована эффективность наносеребра (линейный размер частиц 10-20 нм) в виде запатентованной композиции, которое показало высокую бактерицидную и фунгицидную активность против грам-положительных, грамотрицательных бактерий и кандид в минимальных концентрациях.
Ключевые слова: наночастицы серебра, бактерии, грибы
For citation: Tofan Yu. V., Pavlova N. V., Demyanenko S. A., Kharchenko V. Z., Morozova M. N. EXPERIMENTAL STUDY OF THE BIOCIDAL EFFECT OF NANOSILVER. Medical News of North Caucasus. 2022;17(2):204-206. DOI - https://doi.org/10.14300/mnnc.2022.17050
Для цитирования: Тофан Ю. В., Павлова Н. В., Демьяненко С. А., Харченко В. З., Морозова М. Н. ЭКСПЕРИМЕНТАЛЬНОЕ ИССЛЕДОВАНИЕ БИОЦИДНОГО ДЕЙСТВИЯ НАНОСЕРЕБРА. Медицинский вестник Северного Кавказа. 2022;17(2):204-206. DOI - https://doi.org/10.14300/mnnc.2022.17050
ATCC - American Type Culture Collection MIC - minimum inhibitory concentration
ISO - International Organization for Standardization
The development of nanotechnology has given some antibiotic-resistant strains of microorganisms
impetus to the development of new areas of with good wound-healing properties [1, 2]. Experi-
healthcare, biotechnology, and additive tech- mental data and clinical observations with the possi-
nologies. Among other things, highly effective silver bility of modification of application conditions allow
nanoparticles have been obtained that are more ac- assuming a reduction of quantitative load of silver
tive in the 1-100 nm range than other biocides for nanoparticles, with the case of removal of the po-
medical news of north caucasus
2022. Vol. 17. Iss. 2
медицинский вестник северного кавказа
2022. Т. 17. № 2
tential of undesirable reactions when they are used [2, 3].
The study aimed to determine the minimum bactericidal concentration of the nanosilver preparation for gram-positive, gram-negative bacteria and fungi.
Material and Methods. The activity of a 0.1 % solution of silver nanoparticles with a linear size of 10-20 nm in a matrix of 0.6 % sodium alginate in an aqueous medium (99.3 %) (patent UA N10539) against international test strains of bacteria with a known level of antibiotic resistance was studied: Staphylococcus aureus ATCC 25923; Escherichia coli ATCC 25922; Candida albicans CCM885.
The antibacterial and fungicidal activity was assessed by determining the minimum inhibitory concentrations (MlC) for each microorganism by diluting it in a liquid nutrient medium following ISO 20776-1:2006 and the National Standard GOST R ISO 20776-1-2010.
Monitoring of the experiment results was carried out after 24 hours by two methods: taking into account the formation of biofilms in the studied solution and the method of sowing the studied contents on the peptone agar. The positive result of biofilms was determined as the absence of the formation of a biofilm in a liquid nutrient medium with a bactericidal concentration of the preparation.
The second control of the experiment is the sowing of agar to detect the lack of growth of micro-organisms in a liquid medium containing nanosarbiral, which confirms the bactericide of the drug concentration. The result was taken into account after 24 hours for the presence or absence of growth of colonies on the agar by sectors corresponding to the holes of the tablet with a particular concentration of the drug under study.
The mathematical processing on a personal computer using the statistical program Statistica 6.0 (StatSoft Inc., USA) was applied. The nature of the distribution was determined using the Shapiro - Wilk test, and, with a normal distribution, Student's t-test was used.
Results and Discussion. In the course of the study, the ability to form a biofilm on the surface of a liquid nutrient medium without a studied composition containing silver nanoparticles is shown for all test strains. After 24 hours of exposure to microorganisms in a liquid medium and after passage and incubation on agar, at least 108 CFU/ml of S. aureus, E. coli, or Candida strains were detected, indicating good growth qualities of the studied cultures.
When evaluating the activity of the various concentrations of the composition with silver nanoparticles, the following bactericidal activity values for microorganism control strains were found:
- for S. aureus and E. coli strains, MIC 50/90 values were 0.01-0.09 mg/ml;
- for Candida, MIC 50/90 values were 0.06-0.35 mg/
ml.
Five passages were carried out with each culture of strains of microorganisms according to the proposed method, and in all cases, identical results for each strain were obtained.
The result of biofilm detection in a liquid medium with E. coli culture with the addition of a concentration of 0.01 mg/ml of the nanosilver composition under study can be considered attractive. The subsequent passage on agar of the contents of the medium, however, did not reveal the growth of microorganisms. This fact can be regarded as the presence of Escherichia coli strains in a liquid nutrient medium when exposed to nanosilver at a given concentration, which, at the same time, lose their ability to be cultivated on agar. We can talk about the formation of either a bacteriostatic effect of the studied drug or a new impact of nanosilver particles in relation to E. coli strains.
The MIC 50/90 values for each microorganism control strain studied indicate a significant microbicidal potential for nanomera. Silver in the nanoscale range of 10-20 nm in the matrix of 0.6 % sodium alginate in an aqueous medium has an antibacterial and fungicidal effect at low concentrations, which may be due to the larger specific surface of nanoparticles in this composition and the increased area of contact of nanosilver with microorganisms [4]. Hence the possibility of reducing the toxic effect of silver as a metal a hundred times while maintaining its bactericidal properties, including for the remaining viable but uncul-tivable micro-organisms [2, 5]. Nanosilver offers a wide opportunity to create highly effective drugs and their wide use in medicine and other areas of human activity.
Conclusions. The results show a high bactericidal and fungicidal activity of the composition containing a 0.1 % silver nanoparticle solution with a linear size of 10-20 nm in a 0.6 % sodium alginate matrix in an aquatic medium with respect to staphylococcus strains, Escherichia coli and Candida with a known level of antibiotic resistance.
Financing. The study was not sponsored.
Disclosures: The authors declare no conflict of interest.
References
1. Qamer S., Romli M. H., Che-Hamzah F., Misni N., Joseph N. M. S. [et al.] Systematic Review on Biosynthesis of Silver Nanoparticles and Antibacterial Activities: Application and Theoretical Perspectives. Molecules. 2021;26(16):5057.
https://doi.org/10.3390/molecules2616505
2. Munir T., Mahmood A., Shafiq F., Fakhar-E-Alam M., Atif M. [et al.] Experimental and theoretical analyses of nano-silver for antibacterial activity based on differential crystal growth temperatures. Saudi J. Biol. Sci. 2021;28(12):7561-7566.
https://doi.org/10.1016/j.sjbs.2021.09.058
3. Xiao X., He E. J., Lu X. R., Wu L. J., Fan Y. Y., Yu H. Q. Evaluation of antibacterial activities of silver nanoparticles
on culturability and cell viability of Escherichia coli. Sci.
Total Environ. 2021;794:148765.
https://doi.org/10.1016/j.scitotenv.2021.148765
4. Kemah B., Uzer G., Turhan Y., Özturan B., Kilip B. [et al.] Effects of Local Application of Nano-silver on Osteomyelitis and Soft Tissue Infections: An Experimental Study in Rats. J. Bone Jt. Infect. 2018;3(1):43-49. https://doi.org/10.7150/jbji.22121
5. Dalavi P. A., Prabhu A., Shastry R. P., Venkatesan J. Microspheres containing biosynthesized silver nanoparticles with alginate-nano hydroxyapatite for biomedical applications. J. Biomater. Sci. Polym. Ed. 2020;31(16):2025-2043.
https://doi.org/10.1080/09205063.2020.1793464
About authors:
Tofan Yuliya Vladimirovna, Assistant of the Department of dentistry and orthodontics;
tel.: + 79787715298; e-mail: julia.tofan@yandex.ru; https://orcid.org/0000-0002-1190-596X
Pavlova Natalya Viktorovna, MD, PhD, Associate Professor of the Department of microbiology, virology and immunology; tel.: +79780917246; e-mail: Natalia_Natalia-1@inbox.ru; https://orcid.org/0000-0002-6173-0619
Demyanenko Svetlana Alexandrovna, MD, PhD, Professor, Head of the Department of dentistry and orthodontics; tel.: +79787633301; e-mail: dc.kvalitet@gmail.com; https://orcid.org/0000-0002-2743-498X
Kharchenko Vladimir Zakharovich, MD, PhD, Professor of the Department of general and clinical pathophysiology; tel.: +79787075257; e-mail: mr.vzh43@mail.ru; https://orcid.org/0000-0001-5092-4672
Morozova Marina Nikolaevna, MD, PhD, Professor, Professor of the Department of dentistry and orthodontics; tel.: +79787417438; e-mail: mmrz58@mail.ru; https://orcid.org/0000-0002-4627-925X
© Group of authors, 2022
UDC 617.55.12-007.274-089.843
DOI - https://doi.org/10.14300/mnnc.2022.17051
ISSN - 2073-8137
DEVELOPMENT OF THE ADHESIVE PROCESS IN THE ABDOMINAL CAVITY USING PLASTIC DEVICES IN LAPAROSCOPIC SURGERY
Minaev S. V. 1, Grigorova A. N. 1, Timofeev S. I. 2, Obedin A. N. 1, Gerasimenko I. N. 1, Vladimirova O. V. 1 3, Korablina S. S. 1, Trivailo A. D. 1
1 Stavropol State Medical University, Russian Federation
2 Far Eastern State Medical University, Khabarovsk, Russian Federation
3 City Clinical Hospital № 2, Stavropol, Russian Federation
РАЗВИТИЕ СПАЕЧНОГО ПРОЦЕССА В БРЮШНОЙ ПОЛОСТИ ПРИ ИСПОЛЬЗОВАНИИ ПЛАСТИКОВЫХ ДЕВАЙСОВ В ЛАПАРОСКОПИЧЕСКОЙ ХИРУРГИИ
С. В. Минаев 1, А. Н. Григорова 1, С. И. Тимофеев 2, А. Н. Обедин 1,
И. Н. Герасименко 1, О. В. Владимирова 1 3, C. С. Кораблина 1, А. Д. Тривайло 1
1 Ставропольский государственный медицинский университет, Российская Федерация
2 Дальневосточный государственный медицинский университет, Хабаровск, Российская Федерация
3 Городская клиническая больница № 2, Ставрополь, Российская Федерация
The study examined the risk of developing adhesions in the abdominal cavity using modern plastic devices used in endoscopic interventions. Two equivalent groups were formed out of 80 rats. In the first group, 40 rats were injected with a sterile fragment of classical endoscopic bag through a puncture of the anterior abdominal wall, while in the second one (40 rats) - sterile plastic was used for 3D printing in medicine. The animals were removed from the experiment on the 30th and 90th days. After the macroscopic determination of the development degree of the adhesive process, the parietal and visceral peritoneum was taken out, followed by IHC examination. As a result of the study, it was found that the plastic, which is a part of endo bags and 3D printing plastic, does not lead to the formation of viscero-parietal adhesions associated with the development of the adhesive process. Thus, using various plastic devices for 3D printing in the abdominal cavity is safe. However, additional research is needed.
Keywords: adhesions, abdominal cavity, experiment, endoscopic device, 3D printing, laparoscopy
В исследовании изучался риск развития спаечного процесса в брюшной полости в эксперименте при использовании современных пластиковых девайсов, применяемых при эндоскопических вмешательствах. Из 80 крыс были сформированы 2 равнозначные группы. В I группе - 40 крыс, которым выполняли введение через прокол передней брюшной стенки стерильного фрагмента классического эндоскопического мешка, во II группе (40 крыс) - стерильного пластика, используемого для зD-принтинга в медицине. Животные выводились из эксперимента на 30 и на 90 сутки. После макроскопического определения степени развития спаечного процесса выполняли забор париетальной и висцеральной брюшины с последующим ИГХ-исследованием. В результате проведенного исследования установлено, что пластмасса, входящая в состав эндоконтейнеров и пластика зD-принтинга, не приводит к формированию висцеро-париетальных адгезий, ассоциированных с развитием спаечного процесса. Таким образом, использование в брюшной полости различных девайсов из пластика для 3D-принтинга является безопасным. Однако необходимо проведение дополнительных исследований.
Ключевые слова: спайки, брюшная полость, эксперимент, эндоскопическое устройство, ЭО-принтинг, лапароскопия
For citation: Minaev S. V., Grigorova A. N., Timofeev S. I., Obedin A. N., Gerasimenko I. N., Vladimirova O. V., Korablina S. S., Trivailo A. D. DEVELOPMENT OF THE ADHESIVE PROCESS IN THE ABDOMINAL CAVITY USING PLASTIC DEVICES IN LAPAROSCOPIC SURGERY. Medical News of North Caucasus. 2022;17(2):206-208. DOI - https://doi.org/10.14300/mnnc.2022.17051
Для цитирования: Минаев С. В., Григорова А. Н., Тимофеев С. И., Обедин А. Н., Герасименко И. Н., Владимирова О. В., Кораблина C. С., Тривайло А. Д. РАЗВИТИЕ СПАЕЧНОГО ПРОЦЕССА В БРЮШНОЙ ПОЛОСТИ ПРИ ИСПОЛЬЗОВАНИИ ПЛАСТИКОВЫХ ДЕВАЙСОВ В ЛАПАРОСКОПИЧЕСКОЙ ХИРУРГИИ. Медицинский вестник Северного Кавказа. 2022;17(2):206-208. DOI - https://doi.org/10.14300/mnnc.2022.17051
