CC BY
11plasticity was improved.[8] JD-30 improves deterioration of spatial learning and memory in the SAMP8 mouse model, and by decreasing the content and deposition of AP, neuronal activity and synaptic plasticity improve.[9] A better absorption of paeoniflorin in rats of the VD than in normal group was observed through orally administrating with DSS, which is helpful for the treatment of VD.[10] DSS could ameliorate deterioration of cognition in SAMP8, especially in female animals. Increasing E2, NO, and glycine might contribute to the cognitive improvement effect of DSS in female SAMP8.[11]
Reference
[1] Huang Z, Mao QQ, Zhong XM, Li ZY, Qiu FM, Ip SP. Mechanistic study on the antidepressant-like effect of danggui-shaoy-ao-san, a chinese herbal formula. Evid Based Complement Alternat Med. 2012:173565.
[2] Liu IM, Tzeng TF, Liou SS, Chang CJ.Beneficial effect of traditional chinese medicinal formuladanggui-shaoyao-san on advanced glycation end-product-mediated renal injury in streptozotocin-diabetic rats. Evid Based Complement Alternat Med. 2012:140103.
[3] Zhong S, Ma S, Hong Z, Jin X. Anti-inflammation effect of danggui shaoyao san on Alzheimer's diseases. Zhongguo Zhong Yao Za Zhi. 36(22):3155-60.
[4] Li H, Gu Z, Wu L, Xia L, Zhou K, E L, Wang D, Kou J, Liu H.Danggui-shaoyao-san, a traditional Chinese medicine prescription, alleviates the orthodontic pain and inhibits neuronal and microglia activation.Chin Med J (Engl). 127(20):3630-7.
[5] Yin JB, Zhou KC, Wu HH, Hu W, Ding T, Zhang T, Wang LY, Kou JP, Kaye AD, Wang W. Analgesic Effects of Danggui-Shaoyao-San on Various "Phenotypes" of Nociception and Inflammation in a Formalin Pain Model. Mol Neurobiol. 53(10):6835-6848.
[6] Qu HG, Cheng SW, Tian RB, Li ZL, Lei WL, Wang HQ, Yao ZB, He HW.Effects of the aqueous extract of the Chinese medicine Danggui-Shaoyao-San on rat pineal melatonin synthesis. Neuro Endocrinol Lett. 29(3):366-72.
[7] Fu X, Wang Q, Wang Z, Kuang H, Jiang P. Danggui-Shaoyao-San: New Hope for Alzheimer's Disease.Aging Dis. 7(4):502-13.
[8] Hu ZY, Liu G, Yuan H, Yang S, Zhou WX, Zhang YX, Qiao SY. Danggui-Shaoyao-San and its active fraction JD-30 improve Abe-ta-induced spatial recognition deficits in mice.J Ethnopharmacol. 128(2):365-72.
[9] Hu ZY, Liu G, Cheng XR, Huang Y, Yang S, Qiao SY, Sun L, ZhouWX, Zhang YX. JD-30, an active fraction extracted from Dang-gui-Shaoyao-San, decreases P-amyloid content and deposition, improves LTP reduction and prevents spatial cognition impairment in SAMP8 mice. Exp Gerontol. 47(1):14-22.
[10] Liu J, Wang JS, Kong LY. Comparative pharmacokinetics of paeoniflorin in plasma of vascular dementia and normal rats orally administrated with Danggui-Shaoyao-San or pure paeoniflorin. Fitoterapia. 82(3):466-73.
[11] Huang Y, Hu ZY, Yuan H, Shu L, Liu G, Qiao SY, Sun L, Zhou WX, Zhang YX. Danggui-Shaoyao-San Improves Learning and Memory in Female SAMP8 via Modulation of Estradiol. Evid Based Complement Alternat Med. 2014:327294.
EVALUATED STUDY ON THE LETROZOLE AND ENDOTOXIN(LPS) - INDUCED MODEL OF POLYCYSTIC OVARIAN SYNDROME DUE TO KIDNEY DEFICIENCY-BLOOD STAGNATION IN RATS
YANG Xinmingi, FU dongxue2, YU Huijia2,
(1.Department of Obstetrics and Gynecology, The First Affiliated, Heilongjiang University of Chinese Medicine, Harbin 150040, China ; 2. Heilongjiang University of Chinese Medicine, Harbin 150040, China ; E-mail
[Abstract] Objective:To establish rat models for the study of polycystic ovarian syndrome due to Kidney Deficiency-Blood Stagnation by using letrozole and endotoxin(LPS), and evaluated them comprehensively.Methods:Sixty SD female rats aged six weeks were divided into two groups ,including experimental group of fifty rats and control group of ten rats.Rats were administered letrozole combined with LPS to induce PCOS model.Firstly,the rats in the experimental groups received intragastric administration of letrozole (1mg/kg) for 21 days.After the evaluation, the successful experimental ratsreceived injection of bacterial endotoxin (LPS) (2.5 mg/kg) for four weeks.The changes of morphology, body weight, estrous cycle, ovarian morphology , histology and serum levels of sex hormones, inflammatory factors, blood lipids, blood coagulation and hemorheology in rats were observed.Results:The weight gain of PCOS experimental rats was higher than that of control group( P <0. 05).Vaginal epithelial cells lose cyclical changes. Serum sex hormone (LH, T), fasting insulin (INS) concentration was higher than that of the control group( P <0. 05), serum sex hormone (FSH, E2) level was lower than the control group (P < 0.05).After LPS injection, rats showed weight gain slowed, reduced activity, fur lose luster, dry nails,foul stool, dark purple tongue, thickened and enlarged sublingual vein.Experimental group rats serum triglyceride (TG), low density lipoprotein (LDL), high-density lipoprotein (HDL) levels were higher than control group (P < 0.05), total cholesterol (TC) is significantly higher than control group (P < 0.01);Experimental group rats serum interleukin 6 (IL - 6), tumor necrosis factor (TNF alpha), c-reactive protein (CRP) concentration were higher than control group (P < 0.05);Experimental group rats plasma thrombin time (TT), fibrinogen (FIB) content is higher than that of control group (P < 0.05), prothrombin time (PT), partial prothrombin time (APTT) there was no statistically significant difference (P > 0.05);Rate of platelet aggregation in the rat model of experimental group is higher than thecontrol group (P < 0.05), whole blood viscosity, plasma viscosity were significantly higher than that of control group (P < 0.01).The ovaries of experimental rats seemed paler, larger and poly-cystic,histology showed less granular cell layer, thickened albuginea together with proliferated mesenchymaovarian .In the control group, the color of the ovary was red and the surface was smooth.Conclusion:letrozole and endotox-in(LPS)-induced model of polycystic ovarian syndrome due to Kidney Deficiency-Blood Stagnation in rats is successful.
Keywords : PCOS, Letrozole, Endotoxin, Animal model References:
[1] Wang Wenlai, Liu Meijie, Zhao Hongxia, Wang Shaojun, Wang Zhen, Zhang Fangzhen. Polycystic ovary syndrome of traditional Chinese medicine clinical research [J]. China Herald, 2014,02:308-310.
[2] Hong Shixiang. Nearly 20 years of traditional Chinese medicine and Western medicine combined with the [D]. research literature of Guangzhou University of Chinese Medicine, polycystic ovary syndrome, 2013
[3] Liu Yanli, Jia Liting, Ma Baojing, Wang Quanxian, Wang Peng, Li Weiwei, Zhi Yanfang, Zhu Yanling. To evaluate the song Zuofa established PCOS rat model of [J]. Chinese maternal and child health care, 2011,08:1213-1216.
[4], Sun Xuefei, Hu Huiyuan, Gao Qinghua, Sun Yu, et al.,, et al. Application of vaginal smear method in the identification of physiological cycle in rats [J]. advances in anatomical science, 2014,04:360-363.
[5] Cui Yuqian. Effects of testosterone on pancreatic beta cell insulin secretion and apoptosis and its mechanism [D]. Shandong University, 2012
[6] Xu Xiaojuan, Huang Yinghong, Yao Lijuan, Deng Linwen, Li Wanjing, Wang Qiuxiang, Ho Yunliang. Letrozole and comparative studies of DHEA establishment of kidney deficiency and phlegm characteristic information of obese PCOS rat animal model of [J]. China medicine, 2015,05:511-514+529.
[7] Yang Chao, Zhou Yan, Sun Xiaohong, Ji man, Xu Qunyuan. With noxious heat and blood stasis syndrome characterized the rat blood components and rheological changes of [J]. Chinese Journal of comparative medicine, 2007,10:607-612.
[8] Yang Zhengwang, Zhou Fang, Tan Zhen Yu, Xu Qiongfang, Zhang Shuyi, Zhong Fei, Li Lan, Xiong Juan, Yu Ximing, Lu Yaobang. Effects of different modeling methods on the rat models of polycystic ovary syndrome [J]. Chinese experimental animal journal, 2010,01:13-16+94.
[9] Song Chengcheng, Wang Zhibin, Su Bin, Guo Yudong, Gao Yang, Zuo Zeping, Jin Jiajin. A comparative study on blood stasis model used in rats [J]. Journal of Beijing University of Chinese Medicine, 2014,02:94-98.
TO OBSERVE THE CLINICAL THERAPEUTIC EFFECT OF ACUPOINT CATGUT EMBEDDING THERAPY OF URINANY INCONTINENCE AFTER STROKE WITH SCALP ACUPUNCTURE
YANG Yuanyuan
(The Hanan Branch of the Second Affiliated Hospital of Heilongjiang University Of Chinese Medicine, Harbin,Hei-longjiang 150001) E-mail : [email protected] Tel:18045181662
Abstract Objective To observe the effect of scalp acupuncture foot motor sensory area combined with acupoint catgut embedding and treated with scalp acupuncture foot circulation influence area on the clinical efficacy of urinary incontinence after stroke.Methods Randomly 60 patients with diagnostic criteria were divided into two groups. Scalp acupuncture foot motor sensory area combined with acupoint catgut embedding therapy 30 cases as treatment group, acupuncture scalp foot motor sensory area of 30 cases as control group, to observe the curative effect, the statistical effect after the end of treatment.Results The scalp acupuncture combined with Catgut Implantation at acupoint foot motor sensory area total effective rate (92.3%) was significantly higher than that of simple acupuncture scalp foot motor sensory area group total effectiveness (73.3%), there was significant difference between two groups (P<0.05).Conclusion Acupuncture of foot motor sensory area combined with acupoint catgut embedding therapy clinical curative effect, which is better than the treatment with acupuncture of foot motor sensory area, provides a more reliable clinical evidence for the promotion of acupoint catgut embedding therapy for the disease.
Key words :acupuncture;foot motor sensory area; acupoint catgut embedding therapy; urinary incontinence;
Urinary incontinence is a loss of control under conscious, Urinary incontinence after stroke often shows a strong urine, conscious control of urination and can't stop the urine,urge incontinence, The disease belongs to traditional Chinese medicine "stroke", "urine can not help"category.
Objective In this study, the clinical effect of acupuncture combined with acupoint catgut embedding therapy on urinary incontinence after stroke is achieved, In order to provide a new theoretical basis for the study of clinical disease.
Materials and methods Diagnostic criteria for cerebral vascular disease meeting in Chinese medicine: criteria for evaluation of therapeutic effect of stroke[1]. Treatment group: the acupuncture therapy: The main acupoints Bilateral foot motor sensory area, Fengchi, Hegu, Quchi, Zusanli, taichong. Catgut embedding : Guanyuan, Qihai, Sanyinjiao. Choose the same side of the acupuncture points, alternate use. Embedding operation: Patients take supine position, point skin routine disinfection, 00 ~2 chromic catgut (0.8 ~ 1cm) in the No. 9 needle, the point from the local to the flat below the needle thorn, each point is about 15 ~ 20mm, for lifting thrusting twirling after the gas, The use of Ren's "two fast slow" manipulation [2]. The control group:only patients with the treatment group received acupuncture treatment and acupuncture of Guanyuan, Qihai, Sanyinjiao, do not accept the acupoint catgut embedding therapy. Grading of urinary incontinence before and after treatment. Method for grading the degree of urinary incontinence [3].Clinical Standard:efficacy criteria[4].Treatment outcome:after 4 courses of treatment, the results were compared in table 1.
