CC BY
10# Research Results in Pharmacology
Research Results in Pharmacology 8(4): 115-139
UDC 615.035.1 DOI 10.3897/rrpharmacology. 8.80376
3 Review Article
Endothelial dysfunction: developmental mechanisms and therapeutic strategies
Dmitry V. Shcheblykin1, Anton A. Bolgov1, Mikhail V. Pokrovskii1, Julia V. Stepenko1, Julia M. Tsuverkalova1, Olesya V. Shcheblykina1, Polina A. Golubinskaya2, Liliya V. Korokina1
1 Belgorod State National Research University, 85 Pobedy St., Belgorod 308015, Russia
2 Budgetary Healthcare Institution of the Voronezh Region "Voronezh Regional Clinical Ophthalmological Hospital", 22 Revolutiutsii 1905 goda St. Voronezh 394000, Russia
Corresponding author: Olesya V. Shcheblykina ([email protected])
Academic editor: Oleg Gudyrev ♦ Received 10 January 2022 ♦ Accepted 13 December 2022 ♦ Published 22 December 2022
Citation: Shcheblykin DV, Bolgov AA, Pokrovskii MV, Stepenko JuV, Tsuverkalova JuM, Shcheblykina OV, Golubinskaya PA, Korokina LV (2022) Endothelial dysfunction: developmental mechanisms and therapeutic strategies. Research Results in Pharmacology 8(4): 115-139. https://doi.org/10.3897/rrpharmacology.8.80376
Abstract
Introduction: Every year the importance of the normal functioning of the endothelial layer of the vascular wall in maintaining the health of the body becomes more and more obvious.
The physiological role of the endothelium: The endothelium is a metabolically active organ actively involved in the regulation of hemostasis, modulation of inflammation, maintenance of hemovascular homeostasis, regulation of angio-genesis, vascular tone, and permeability.
Risk factors for the development of endothelial dysfunction: Currently, insufficient bioavailability of nitric oxide is considered the most significant risk factor for endothelial dysfunction.
Mechanisms of development of endothelial dysfunction: The genesis of endothelial dysfunction is a multifactorial process. Among various complex mechanisms, this review examines oxidative stress, inflammation, hyperglycemia, vitamin D deficiency, dyslipidemia, excess visceral fat, hyperhomocysteinemia, hyperuricemia, as well as primary genetic defect of endotheliocytes, as the most common causes in the population underlying the development of endo-thelial dysfunction.
Markers of endothelial dysfunction in various diseases: This article discusses the main biomarkers of endothelial dysfunction currently used, as well as promising biomarkers in the future for laboratory diagnosis of this pathology.
Therapeutic strategies: Therapeutic approaches to the endothelium in order to prevent or reduce a degree of damage to the vascular wall are briefly described.
Conclusion: Endothelial dysfunction is a typical pathological process involved in the pathogenesis of many diseases. Thus, pharmacological agents with endothelioprotective properties can provide more therapeutic benefits than a drug without such an effect.
Keywords
endothelium, endothelial dysfunction, biomarkers, therapeutic strategies.
Copyright Shcheblykin DV et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Introduction
Since the discovery in 1980 that acetylcholine-mediat-ed vasodilation requires the presence of endothelial cells (Furchgott and Zawadzki 1980), the importance of the endothelial layer of the vascular wall for vascular ho-meostasis has become increasingly apparent. Endothelial dysfunction is an integral part of the pathophysiology of various diseases, including hypertension, atherosclerosis, chronic heart failure, coronary heart disease (CHD), diabetes mellitus, chronic renal failure, oncological diseases and even mental disorders. Therefore, in order to develop therapeutic strategies, it is necessary to understand the key factors involved in maintaining endothelial function and the signaling pathways affecting endothelial dysfunction (ED).
The purpose of this article is to describe the function and main signaling pathways of oxidative stress, inflammatory factors, as well as metabolic disorders leading to the development of ED, to consider currently available biochemical markers of ED, as well as promising therapeutic strategies for the prevention and treatment of this pathology.
The physiological role of the endothelium
Blood vessels consist of connective tissue, fibroblasts, endothelial cells, and vascular smooth muscle cells. The inner surface of the blood vessel wall is covered with en-dothelium, which is a semi-permeable continuous mono-layer of flat cells of mesenchymal origin between the bloodstream and the vessel wall. Due to dense specialized intercellular connections, the endothelium forms a barrier that selectively restricts the movement of macromole-cules (Rahimi 2017). The barrier plays an important role in maintaining vascular tone, fluid homeostasis, and body protection (Zhang et al. 2018).
For many years, the endothelium was considered only to be a cellular barrier. But numerous studies have shown a much more complex role of the endothelium. The endothelium is not only a highly selective barrier, but also a metabolically active system, not least involved in maintaining vascular homeostasis, regulating the balance between vasodilation and vasoconstriction (Kasprzak et al. 2006). Vasoconstriction is provided by the following factors: endothelin-1 (ET-1), angiotensin II, prostaglan-din H2, and thromboxane A2 (Bonetti et al. 2003). Nitric oxide (NO) (formerly known as endothelial relaxation factor), endothelin depolarization factor (EDHF), prosta-cyclin or natriuretic peptides are responsible for vasodila-tion (Busse and Fleming 2006; Moncada and Higgs 2006; Spieker et al. 2006). Vasodilating endothelial factors also have antiaggregational properties, inhibit the formation of blood clots, vascular stenosis and, in the case of NO/ cGMP (cyclic guanosine monophosphate), also prevent myocardial hypertrophy (Ritchie et al. 2009).
Not so long ago, hydrogen sulfide (H2S) was identified as a new vasodilator produced by endothelial cells (Yang et al. 2008) that acts in conjunction with NO (Cortese-Krott et al. 2015; Yuan et al. 2015). Regulation of vascular tone by H2S is carried out both by direct action on vascular smooth muscle cells and by endothe-lium-dependent pathway (Wang et al. 2015). The main mechanism of H2S-induced vasodilation is associated with the activation of ATP-sensitive K+(ATP) channels of vascular smooth muscle (Zhao et al. 2001). The involvement of K+ATP channels in the H2S-mediated regulation of vascular tone is further confirmed by the effects of vasodilation blockade by the K+ATP channel inhibitor glibenclamide (Webb et al. 2008). In addition, H2S potentiates the NO-mediated vasodilating effect by inhibiting phosphodiesterase-5 (PDE-5), prolonging the half-life of cGMP, which is a key mediator in vasoactive NO signals (Bucci et al. 2010).
Under physiological conditions, due to the controlled synthesis and release of biologically active substances (Table 1) (Melnikova and Makarova 2015), the endo-thelium has the ability to maintain a balance between its multidirectional functions: regulation of the hemostasis system, modulation of inflammation, maintenance of hemovascular homeostasis, regulation of angiogenesis, vascular tone, and permeability.
Table 1. Factors synthesized in the endothelium determining its functions (Melnikova and Makarova 2015)
Group Function Biologically active substances
Factors narrowing of blood endothelin-1, angiotensin II,
affecting vessels thromboxane A2, prostaglandin H2
vascular dilation of blood nitric oxide, prostacyclin, endothelin
smooth vessels depolarization factor, angiotensin I,
muscle tone adrenomedullin, hydrogen sulfide
Hemostasis prothrombogenic platelet growth factor, plasminogen
factors tissue activator inhibitor, Willebrand factor (blood clotting factor VIII), angiotensin IV, endothelin-1, fibronectin, thrombospondin, platelet activation factor
antithrombogenic nitric oxide, tissue plasminogen activator, prostacyclin, thrombomodulin
Factors stimulants endothelin-1, angiotensin II,
affecting superoxide radicals, endothelial
growth and growth factor
proliferation inhibitors nitric oxide, prostacyclin, C-type natriuretic peptide, heparin-like growth inhibitors
Factors pro-inflammatory tumor necrosis factor alpha,
affecting superoxide radicals, C-reactive
inflammation protein
anti-inflammatory nitric oxide
The imbalance of these diverse vasoactive factors is fundamental to the development of ED (Frey et al. 2009; Tarafdar and Pula 2018). According to some data, glyco-calyx plays an equally important role, located on the surface of endothelial cells facing the vascular bed and participating in the adhesion of leukocytes and platelets and,
consequently, in the function of the endothelium (Van den Berg et al. 2006). It should be emphasized that areas of the damaged endothelium can be renewed thanks to circulating endothelial progenitor cells (EPC), the qualitative and quantitative indicators of which significantly affect the outcomes and prognoses of cardiovascular diseases (Werner et al. 2005) and the functioning of the endothelium as a whole (Hill et al. 2003).
According to many researchers, just assessing the function of the endothelium may not be enough, since the functioning of the vascular wall cannot be considered in isolation from the functional state of the smooth myocytes that make it up (for example, a degree of activity of soluble guanylyl cyclase) (Stasch et al. 2006). Moreover, it is currently proven that the adipose tissue surrounding the vessels contributes to vascular homeostasis by producing vasoactive compounds such as adipokines, reactive oxygen species (ROS) and NO (Brown et al. 2014; Jankovic et al. 2017).
Risk factors for ED development
The clinical characteristic of ED is a violation of en-dothelium-dependent vasorelaxation in response to factors such as exposure to acetylcholine and bradykinin, or changes in blood flow. Although the mechanisms leading to ED are numerous, the main one, nevertheless, is a decrease in the bioavailability of NO. The
diagram (Fig. 1) shows that the reason for a decrease in the bioavailability of NO may be a decrease in the availability of L-arginine (Schlaich et al. 2004), the accumulation of asymmetric dimethylarginine (ADMA) (Gamil et al. 2020), changes in the interaction with heat shock protein 90 (Hp90) (Ou et al. 2003), and phosphorylation of endothelial NO synthase (eNOS) (Smith and Hagen 2003), as well as an increase in NO uptake due to an excessive amount of ROS accumulated due to the activity of NADPH and xanthine oxidases (Frey et al. 2009; Tarafdar and Pula 2018), and the separation of eNOS. In addition, it should be noted that changes in caveolin-1 (Darblade et al. 2001), tetrahydrobiopterin (BH4) (Topal et al. 2004), S-glutathionylation of ENOS (Chen et al. 2010; Kavoussi et al. 2019), and oxidation of low-density lipoproteins (oxLDL) (Fleming et al. 2005) are also involved in the separation of eNOS. It is important that a decrease in the expression of the eNOS protein also leads to a violation of the activity of eNOS and the production of NO, which are often observed in such cardiovascular diseases as atherosclerosis, acute myocardial infarction and heart failure in animals and humans (Fujii et al. 2002; Damy et al. 2004; Tonduangu et al. 2004).
The mechanisms underlying a decrease in the bio-availability of nitric oxide include both a decrease in the production of nitric oxide and increased absorption of nitric oxide. A decrease in NO production may be the result of:
Figure 1. Mechanism of reducing the bioavailability of nitric oxide (Su 2015). Notes: NO - nitric oxide, ENOS - endothelial NO-synthase, CAM - calmodulin, ADMA - asymmetric dimethylarginine, DDAH - dimethylarginindimethylaminohydrolase BH4 -tetrahydrobiopterin, P - receptor, G - G-protein, Hsp90 - heat shock protein 90, PI3K - phosphatidylinositol-3-kinase, Akt - protein kinase B, NADPH is the reduced form of nicotinamide adenine dinucleotide phosphate, SOD - superoxide dismutase, ONOO - per-oxynitrite, O2 - superoxide, O2 - oxygen, H2O2 - hydrogen peroxide, EC - endothelial cells, MMC - smooth muscle cells (Su 2015).
• reduced availability of L-arginine due to its deficiency, disturbances in its transporter, or due to a combination of these phenomena;
• accumulation of ADMA, which is an endogenous eNOS inhibitor;
• deficiency of the BH4 cofactor or its modification;
• increases in caveolin-1, leading to a change in the interaction between it and eNOS;
• changes in G-proteins associated with the receptor;
• changes in the interaction of eNOS with Hsp90 due to changes in the content of Hsp90;
• changes in calcium-independent phosphatidylinosi-tol-3-kinase (PI3K) / protein kinase B (Akt) - mediated activation of eNOS due to tyrosine or serine phosphorylation;
• as well as a decrease in eNOS expression as a result of a decrease in the transcription of the eNOS gene and/or a decrease in the stability of the eNOS mRNA.
Increased absorption of NO by ROS and reactive forms of nitrogen (RFA) may be associated with:
• separation of eNOS associated with changes in BH4, caveolin-1 and oxLDL;
• increased expression of NADPH and its activity;
• and increased expression and activity of xanthine oxidase.
The mechanisms underlying ED in various diseases may vary according to the factors contributing to the development of each specific disease.
Mechanisms of ED development
Under physiological conditions, vasodilation, synthesis of aggregation inhibitors, coagulation and fibrinolysis activators, anti-adhesive substances by endothelial cells prevail. ED implies a violation of the production of various messengers produced by the endothelium (as well as smooth muscles, perivascular adipose tissue), which leads to a vasoconstrictor, proinflammatory and proath-erothrombotic phenotype, leading to a violation of the regulation of vascular tone and vascular permeability.
The development of ED is a multifactorial process. Among the many complex mechanisms, oxidative stress is probably the most common cause underlying the development of ED. Oxidative stress develops with excessive formation of free radicals and insufficient mechanisms of antioxidant defense systems. Most risk factors for cardiovascular diseases also contribute to the development of intracellular oxidative stress and increased production of ROS, which contributes to the activation of NADPH oxidase, inactivation of NO, formation of peroxynitrite (ONOO-), inhibition of eNOS activity, stimulation of en-dothelin expression, etc. (Forstermann and Munzel 2006).
Another common mechanism for the development of endothelial dysfunction is inflammation, and there is a
high probability that there is a direct relationship between the inflammatory process and oxidative stress (Karbach et al. 2014). Under normal conditions, the endothelium regulates vascular inflammation by releasing NO. However, the damaged endothelium contributes to the formation of ROS and thereby exacerbates vascular inflammation, which adversely affects the vascular wall. Oxidative stress can increase inflammation of the vascular wall, and inflammatory cells increasingly secrete superoxide radicals (Karbach et al. 2014), potentiating oxidative stress. There are many inflammatory markers associated with ED. C-reactive protein (CRP) is an acute-phase inflammatory protein, which is released in response to various types of inflammation. Experimental studies have shown that CRP directly contributes to the early phase of atherosclerosis due to deposition on the intima, even before the appearance of monocytes (Torzewski et al. 2000). In addition, CRP directly affects the bioavailability of NO, which leads to the development of oxidative stress, and as a consequence of ED and intimal hyperplasia. CRP acts directly through a lec-tin-like oxidized low-density lipoprotein receptor, which plays a crucial role in oxLDL-induced ED in human aortic endothelial cells (Li et al. 2004). Inflammation is also associated with overexpression of tumor necrosis factor-alpha (TNF-a) and interleukin-1, which contribute to the attachment and migration of leukocytes (Barton 2013). In addition, these inflammatory cytokines induce endothelial cells and leukocytes to express adhesion molecules such as vascular cell adhesion molecules (VCAM) and intercellular adhesion molecules (ICAM), chemotactic monocyte protein-1, E-selectin, P-selectin and interleukin-6, which leads to increased ED (Blake and Ridker 2001).
The endothelial function can also be disrupted by infectious agents and immuno-mediated damage developing due to their effects (Epstein et al. 2000). According to population studies, infectious agents can cause predisposition of patients to cardiovascular diseases and their clinical manifestations. Viruses, such as cytomegalovirus and herpes simplex virus-1 and bacteria such as Chlamydia pneumoniae and Helicobacter pylori, are reported to be associated with the development of various forms of coronary heart disease in humans (Pothineni et al. 2017). It was shown that the titers of immunoglobulin-G antibodies to cytomegalovirus, hepatitis A virus, herpes simplex virus-1, Chlamydia pneumonia and Helicobacter pylori were independent risk factors for ED and the presence of coronary heart disease. Therefore, it is possible that due to infection with these pathogens, common significant immunological pathways may develop, as a result of activation of which endothelial damage occurs and the development of pathological conditions mediated by ED (Grabczewska et al. 2006).
In light of recent events, there are more and more reports that COVID-19 affects organs other than the lungs, primarily the heart and kidneys (Fanelli et al. 2020; Yang et al. 2020). Due to the tissue tropism of SARS-CoV-2 for cells expressing angiotensin converting enzyme 2 (APF2), another major important target for infection is the
vascular endothelium (Monteil et al. 2020). Studies show that APF2 is abundantly expressed on vascular endothelial cells of both small and large arteries and veins (Hamming et al. 2014) demonstrated the structures of virus inclusion in the endothelial cells of glomerular capillary loops and signs of widespread endotheliitis in the heart, lungs, kidneys, liver and gastrointestinal tract in pathological samples from patients with severe COVID-19 (Varga et al. 2020). Damage to the endothelium and its dysfunction in coronavirus infection may be the result of direct infection with SARS-CoV-2 (for example, by induction of intracellular oxidative stress (Khomich et al. 2018), as well as due to a deep systemic inflammatory reaction.
In addition, hyperglycemia is known to disrupt en-dothelial function (Avogaro et al. 2008). Interestingly, even in subjects with normoglycemia who have a high risk of diabetes and insulin resistance, ED was observed during an oral glucose tolerance test (Title et al. 2000). The mechanisms of ED in patients with diabetes melli-tus are associated with a decrease in NO synthesis and increased production of vasoconstrictors (Johnstone et al. 1993). The results of scientific studies show that in patients with diabetes, oxidative stress, NADPH oxidase and eNOS dissociation play an important role in the development of ED (Hsueh et al. 2004). Hyperglycemia also leads to the formation of advanced glycation endproducts (AGE), which are products of non-enzymatic glycation of proteins and lipids. AGEs accumulate in the vessel wall, alter the structural integrity of the endothelium and basement membrane and are able to interfere with the activity of NO. This significantly contributes to the development of ED. In addition, AGEs bind to specific surface receptors that are expressed on cells such as monocytes, macrophages and vascular smooth muscle cells, which leads to an increased inflammatory response, increased vascular permeability and oxidative stress (Soldatos et al. 2005; Avogaro et al. 2008). Vitamin D deficiency (1a, 25-di-hydroxycholecalciferol) becomes a new candidate among the causes of ED development. Vitamin D appears to be indirectly involved in the development of endothelial dysfunction and systemic inflammation. The presence of vitamin D receptors on the surface of endothelial cells has been shown (Kassi et al. 2013). In patients with sub-clinical atherosis and slow coronary blood flow, a strong association was found between vitamin D deficiency and ED (Oz et al. 2013). There are reports in scientific papers that vitamin D stimulates the production of NO in endothelial cells by activating eNOS (Wong et al. 2010; Khan et al. 2018). Recent experimental studies in vivo and in vitro allow us to say with confidence that the vitamin D analog (22-oxacalcitriol) significantly suppresses the increased expression of NADPH oxidase, while increasing ENOS binding, thereby reducing oxidative stress in the endothelium (Oz et al. 2013). Moreover, vitamin D protects endothelial cells from oxidative stress, preventing the formation of superoxide radicals, and apoptosis.
Also, some studies say that high cholesterol, LDL and low HDL levels are independently associated with ED and
inflammation (Mineo et al. 2006). In patients with hypercholesterolemia, a violation of the activation of the metabolic pathway of L-arginine (substrate NO) was found (Hadi et al. 2019). In patients with coronary artery disease and dyslipidemia, it was suggested that increased degradation of NO under the action of ROS, since the infusion of L-arginine partially normalized the impaired function of the coronary endothelium (Cziraki et al. 2020). Possible mechanisms underlying dyslipidemia-induced ED include: 1) activation of NADPH oxidase, development of oxidative stress and increased production of superoxide radicals (O2-); 2) increased plasma ADMA levels; and 3) LDL oxidation. ADMA is an endogenous eNOS inhibitor, which competes with L-arginine for the same binding site on eNOS, which leads to eNOS dissociation. As a result, O2 production increases and NO production decreases (Antoniades et al. 2009). OxLDL cause vasoconstriction by suppressing the action of endothelium-dependent vasodilators and increasing endothelin expression; stimulate proliferation and migration of smooth myocytes, proliferation and subendothelial infiltration of monocytes; increase the production of adhesive molecules by endothelial cells (ICAM, VCAM), stimulate aggregation and adhesion to endothelial cells of leukocytes and platelets, increase coagulation activity of the endothelium, inducing the release of tissue factor and inhibiting fibrinolysis; stimulate the formation of ROS, increase apoptosis, etc. (Markov 2005).
Excess visceral fat is also one of the most important factors in the occurrence and progression of ED. The idea of adipose tissue as an organ of accumulation and storage of energy substrates has finally become a thing of the past. Today, adipose tissue is considered as an important organ that synthesizes many hormone-like proteins. Among the adipocytokines synthesized by visceral fat, which play an important role in the pathogenesis of metabolic syndrome, TNF-a is known to disrupt the function of endothelial cells by increasing insulin resistance and its pro-inflammatory effect (Oz et al. 2013). Also, a number of clinical studies have shown that excess visceral fat is accompanied by a decrease in adiponectin synthesis. Adiponectin is a protein with antiarteriosclerotic activity, the receptor of which is located in vascular endothelial cells. NO is released when endothelial cells are stimulated by adiponectin, which is realized by activation of eNOS by adiponectin via AMP-activated protein kinase and the PI3K/Akt system (Deng et al. 2010). In patients with visceral obesity, as well as with diabetes mellitus, in whom the concentration of adiponectin is reduced, the endothe-lial function is weakened (Szydelko et al. 2020).
An unconventional factor leading to endothelial dysfunction is hyperhomocysteinemia. This is evidenced by animal models of hyperhomocysteinemia (Virdis et al. 2003). There are scientific data on the development of ED in patients with hyperhomocysteinemia without hypertension (Baszczuk et al. 2014). Studies on cells (Zhang et al. 2000), animals (Virdis et al. 2003; Khadieva et al. 2019) and humans (Salvio et al. 2021) show that homocysteine reduces the bioavailability of NO due to oxidative excess.
Through inhibition of NO, production of prostanoid vasoconstrictors, inhibition of EDHF (Heil et al. 2004; Cheng et al. 2011), homocysteine causes activation of the angiotensin 1 receptor (AT1) and generation of ROS (Cheng et al. 2009). It also inhibits ENOS by increasing ADMA production by reducing dimethylarginindimethylamino-hydrolase (DDAH) activity (Stuhlinger et al. 2001) and eNOS dissociation by reducing intracellular synthesis of BH4 (Topal et al. 2004), which leads to a decrease in bioavailability of NO and an increase in ROS formation. In addition, homocysteine suppresses eNOS expression in human endothelial cells (Zhang et al. 2007), causes endothelial damage, vascular deendothelization, and increases platelet adhesion (Harker et al. 1976). Homocysteine also increases the formation of ROS due to phosphorylation of NADPH oxidase (Siow et al. 2006), as well as due increasing the activity of ACE as a result of homo-cysteinylation of ACE with the formation of angiotensin II, which activates NADPH oxidase (Huang et al. 2015). Increased cardiovascular risk in patients with hyperho-mocysteinemia may be explained by the mechanisms described above. This is especially important for patients with chronic renal failure, who often have elevated homo-cysteine levels, which, as shown in recent studies, makes it possible to predict cardiovascular outcomes (Zhang et al. 2020; Shih et al. 2021).
Impaired endothelial cell function is also associated with high plasma uric acid levels (Tomiyama et al. 2011; Maruhashi et al. 2018). The pathological effect of uric acid on endothelial cells is apparently promoted by a number of different surface membrane carriers of urates, such as Glut-9 and URAT-1 (Price et al. 2006; Liu et al. 2017). As a result, the formation of chemokines and ROS is stimulated, as well as the activation of adhesion molecules in combination with increased activity of NF-kB (kappa-bi nuclear factor) and a decrease in NO production, which contributes to the development of ED (Cai et al. 2017; Afonasyeva 2019; Yang et al. 2019).
To date, there is a lot of evidence that the cause of ED may be a primary genetic defect, as indicated by structural changes in the eNOS gene in Japanese patients with essential hypertension (Markov 2005), as well as a decrease in the synthesis of NO in the vessels and their endothe-lium-dependent dilation in adolescents with primary hypertension long before the first symptoms of the disease (Markov 2005) (apparently due to a genetic defect of en-dotheliocytes) (Panza et al. 1993). In addition, it became known that a violation of L-arginine-dependent synthesis of NO can be observed in individuals with normal blood pressure levels, which is interpreted as a primary genetic defect underlying the development of ED. According to some authors, a defect in the genes responsible for the production and intensity of degradation of endotheli-um-relaxing factors may potentiate the formation of ED. Thus, the nature of the primary factor contributing to the occurrence of ED is not completely clear.
There is a judgment that endothelial dysfunction, especially in hypertension, is probably associated with a
violation of the phosphatidylinositol signaling pathway/ Ca2+. Since calcium ionophores stimulate the synthesis of NO by increasing the entry of calcium into endothelial cells regardless of the activation of membrane receptors, the presence of a structural defect or functional failure of these receptors or signaling mechanisms activated by these receptors in patients with arterial hypertension cannot be ruled out (Markov 2005).
Thus, the literature data indicate a complex pathogen-esis of ED. All these factors are closely interrelated, and their effect on the endothelium is difficult to separate. The clinical significance of ED is associated with its role in the development and/or progression of many diseases, not only of the cardiovascular system (Sun et al. 2020; Little et al. 2021), but also rheumatological (Murdaca et al. 2012), oncological (Toya et al. 2020; Ching et al. 2021), mental disorders (Morris et al. 2020) and many others (Vairappan 2015; Ekeloef et al. 2020; Joffre et al. 2020). This prevalence of endothelial dysfunction suggests that it is a common link in the pathogenesis of almost all diseases. Probably, from these positions, ED can be put on a par with such universal damage mechanisms as activation of the immune system and inflammation, formation of ROS and oxidative stress, and some others. There are practically no diseases in which the above mechanisms are not activated to a greater or lesser extent. In addition, they are closely interrelated, including being the cause of ED.
Markers of endothelial dysfunction in various diseases
To assess ED, functional diagnostic methods have been developed, such as flow-mediated dilation, laser Doppler flowmetry, occlusive plethysmography, pulse wave propagation velocity measurement, and in vitro methods using endothelial cell cultures and isolated organs (Storch et al. 2017; Soldatov et al. 2018; Martins-Filho et al. 2020). However, functional methods are very time-consuming compared to laboratory methods for measuring the concentration of biomarkers in blood serum.
Currently, for the purpose of laboratory diagnosis of ED, a number of factors synthesized by endotheliocytes are being considered. The change in the level of these factors in biological fluids reflects a violation of the main functions of the endothelium: vasomotor, regulation of angiogenesis, barrier, adhesion and thromboresistance.
Obviously, to assess the function of the endothelium, it is possible to measure the concentration of NO metabolites, which is constantly released and metabolized into stable nitrite and nitrate ions. However, when studying the NO level in systemic arterial hypertension, ambiguous results were obtained (Vasina et al. 2017). The next marker of endothelial dysfunction is ADMA, which is a structural analog of L-arginine and inhibits the activity of all isoforms of NO synthase, disrupting the formation of nitric oxide in the endothelium (Zhang et al. 2017).
It is known that ADMA is a competitive analog of argi-nine; therefore, a decrease in the L-arginine/ADMA ratio is also very significantly associated with an increase in cerebrovascular risk (Tousoulis et al. 2015).
ET-1, a biologically active broad-spectrum peptide, can serve as another marker of ED reflecting the state of vasomotor function of the endothelium (Stepanova et al. 2019). ET-1 is expressed mainly in the endothelium (Davenport et al. 2016), and it is a factor that intensively stimulates vasoconstriction and mitotic activity of vascular muscle cells, fibroblasts, and cardiomyocytes. It also plays the role of a regulator of endothelial proliferation, stimulating endotheliocyte hyperplasia in low concentrations and their production of NO and prostacyclin. ET-1 serves as one of the significant markers of ED in a number of diseases: chronic and acute ischemic lesions of the brain and myocardium (Sapira et al. 2010), diabetes mel-litus and its vascular complications (Sánchez et al. 2014; Sorrentino et al. 2018; Efimenko et al. 2022). In addition, this peptide is considered as a predictor of the severity and outcome of these pathological conditions.
Vascular Endothelial Growth Factor (VEGF) is known for its role in angiogenesis, promoting proliferation and migration of endothelial cells and increasing vascular permeability. VEGF is produced by endothelial and some other cells. The selective mitogenic activity of VEGF against endothelial cells suggests that its level in the blood can serve as one of the criteria for ED (Gershtein et al. 2015). At the same time, VEGF is a vital factor in the trophism of endothelial cells (Polverino et al. 2018), and an increase in its level in the blood can be compensatory, thereby indicating a favorable course of the pathological process, for example, in stroke (Gontschar et al. 2013).
Expression of the VEGF type 2 receptor (VEGFR-2) is limited to vascular endothelial cells. VEGFR-2 plays an important role in cell migration, endothelium-dependent vasodilation and angiogenesis, which makes it a good candidate for an ED marker (Lange et al. 2016).
Vasogibin-1 (VASH1) is a new, currently actively studied marker of ED, which is formed in endothelial cells under the action of VEGF and the main fibroblast growth factor. It has been experimentally proven that VASH1 prevents premature aging of endothelial cells and increases their resistance to stress (Miyashita et al. 2012), and with replicative aging of endotheliocytes, VASH-1 expression is significantly reduced (Takeda et al. 2016). For example, with the instability of atherosclerotic plaques of the carotid arteries, the production of this protein increases, reflecting active vascularization and inflammation and indicating the risk of ischemic stroke.
Endothelial cells play a key role in the transport of other cell types and metabolic substrates between the blood and the interstitial space. When these cells are activated by pro-inflammatory proteins, such as C-reactive protein, bacterial endotoxins, interleukin 1B, TNF-a, the expression of cell adhesion molecules, such as ICAM-1, VCAM-1 and E-selectin, increases. Transendothelial migration of leukocytes is realized through these molecules.
ICAM-1 is a member of the supergene family of immunoglobulins and a ligand for integrin ß2 molecules present on leukocytes (Hubbard and Rothlein 2000), and is highly expressed in endothelial cells and subendothelial macrophages (de Lemos et al. 2000). ICAM-1 mediates a number of intercellular interactions, including adhesion and migration of leukocytes to the vascular endothelial wall. Literature data support the hypothesis that ICAM-1 expression activates endothelial cells and leads to inflammation, which, in turn, is an important stage for the initiation and progression of atherosclerosis (Marzolla et al. 2017).
VCAM-1 expression is limited to endothelial cells (but sometimes to spindle cells). Unlike ICAM-1, which is produced in low concentrations, VCAM-1 is not expressed in healthy endothelial cells. It is assumed that VCAM-1 expression can lead to endothelial activation, since it increases monocyte recruitment and improves monocyte-endothelial interaction at the initial stages of atherosclerotic lesion formation (Habas and Shang 2018).
E-selectin belongs to the lectin family, participates in the attraction of leukocytes to the site of inflammation and is expressed by vascular endothelial cells, due to which it is a specific marker of endothelial activation (Hidalgo et al. 2007).
Markers of inflammation of CRP, CD40 ligand (CD40L), IL-18, chemotactic protein of monocytes 1, leading to endothelial activation, can act as markers of ED (Straface et al. 2010), since inflammation is an integral part of the development of atherosclerosis.
The endothelial damaging factor, in addition to CRP, is homocysteine, an amino acid formed in the body during methionine metabolism. Elevated homocysteine content is currently considered as an independent risk factor for the development of cardiovascular diseases (Afonasye-va 2019). Hyperhomocysteinemia is also noted in stroke (Murmu et al. 2018) and other pathological conditions, causing the development of ED.
8-hydroxy-2'-deoxyguanosine is a modified nucleoside that is a product of oxidative DNA damage caused by the action of ROS. Recent studies suggest that this compound can be considered as a fairly sensitive and specific early marker of ED in many pathological conditions, including malignant neoplasms, atherosclerosis, cardiovascular diseases and diabetes mellitus (Mahat et al. 2018).
Scientists suggest a direct relationship between elevated levels of circulating endothelial cells (CEC) in peripheral blood and a degree of endothelial damage in patients with atherosclerotic disease and vascular inflammation (Szmitko et al. 2003). Consequently, the amount of CEC measured by flow cytometry can serve as a marker of ED.
The enzyme myeloperoxidase can bind to glycosami-noglycans in the walls of blood vessels and disrupt the release of endothelial NO, which leads to local ED. Scientists have also recognized the role of this enzyme in the occurrence and development of atherosclerosis (Pen-nathur and Heinecke 2007).
Markers of endothelial thromboresistance disorders include Willebrand factor and thrombomodulin, which are synthesized directly in the endothelium (Leite et al. 2020).
Endocan, or endothelial cell-specific molecule-1 (bio-marker of ED), is a proteoglycan dermatan sulfate and is secreted in response to inflammation not only by vascular endotheliocytes, but also by epithelial cells that line the distal tubules of the kidneys and bronchi (Zhang et al. 2012). On the one hand, endoxane increases VEGF-A expression and the interaction between VEGF-A and its VEGFR 2, increasing vascular permeability (Lee et al. 2014), and on the other hand, VEGF directly induces en-doxane expression (Sun et al. 2019).
Another marker of ED is free fatty acids (FFA). FFA can increase ROS levels by increasing cytokine production in mononuclear cells. In addition, FFA can induce activation of the proinflammatory pathway NF-kB in human endothelial cells. In this regard, FFA is considered an early biomarker of ED and atherosclerosis, which is important for the prevention and treatment of cardiovascular diseases (Badimon et al. 2012).
Along with the well-known role of the endothelium in the pathogenesis of cardiovascular diseases, it has been demonstrated that the proper functioning of the endothelium plays a role in human fertility (Santi et al. 2021). Since polycystic ovary syndrome is associated with the risk of cardiovascular diseases, early detection of ED is of clinical importance. In this case, scientists consider increased VEGF, matrix metallopeptidase 9, visfatin, pentraxin-3, and soluble lectin-like oxidized low-density lipoprotein receptor-1 as markers of ED (Dambala et al. 2019).
Thus, the assessment of ED using various markers should be complex, since not all of the compounds listed above have equally valuable prognostic significance. Therefore, the development of an optimal protocol for the diagnosis of ED is an urgent task for fundamental medicine at the present time. As ED indicators become clinically applicable, this may lead to an improved risk assessment methods that will help predict, prevent and treat cardiovascular diseases.
Therapeutic strategies
Evaluation of ED as a result of an imbalance in the formation of vasoactive messengers requires the development of new therapeutic approaches to the effect on the endo-thelium in order to prevent or reduce a degree of damage to the vascular wall.
Currently, it has been proven that the modification of a person's lifestyle and habits lead to an improvement in endothelial function. ED is one of the main damages caused by cigarette smoke (as well as vapors of electronic cigarettes and hookahs (Munzel et al. 2020)). Toxic substances that enter the bloodstream during smoking (for example, free radicals and reactive glycation products) can react with endothelial and cause various vascular damage (Prasad et al. 2015; Munzel et al. 2020). Cigarette smoking causes inflammation and also increases ROS production and lipid peroxidation (Yamaguchi et al. 2005; Barbieri et al. 2011). Cigarette smoke extracts
inhibit the activity of eNOS endothelial cells of the pulmonary artery by changing the pattern of phosphorylation of eNOS (Wagner et al. 2007), which leads to a decrease in the bioavailability of NO. Quitting smoking leads to an improvement in endothelial function (Delgado et al. 2020; Fukumoto et al. 2021).
Physical activity improves the condition of the endothelium (Hirata et al. 2013). Physical exercise causes an increase in NO production, both in normotonics and in patients with hypertension (Nystoriak and Bhatnagar 2018).
Weight loss also contributes to the restoration of endothelial function in obese patients. This is probably facilitated by the normalization of the production of cytokines released from visceral fat (Ziccardi et al. 2002).
Daily monitoring of blood glucose levels is one of the most important factors in ED correction (Chen et al. 2021). In turn, compensation for hyper- and dyslipidemia contributes to normalization of endothelial function (Kim et al. 2012) and reduction of cardiovascular risk (Chen et al. 2021).
An excessive amount of fat consumed daily in food stimulates the development of the initial signs of ED in practically healthy individuals. It has been experimentally shown that a high-fat diet stimulates the formation of oxygen free radicals (superoxide anions) that inactivate NO (Roberts et al. 2000). High salt intake suppresses the effect of NO in peripheral resistive vessels in animal hypertension modeling (Kurtz et al. 2018). A link between reduced NO production and high salt intake in clinical studies in patients with hypertension has also been demonstrated (Li et al. 2009; Boegehold 2013).
Polyunsaturated fatty acids, antioxidants, vitamins (especially, tocopherol and ascorbic acid), folic acid, and L-arginine have been found to have a beneficial effect on vascular endothelial function (Konovalova et al. 2019; Belenichev et al. 2021). They improve endothelium-de-pendent vasodilation both in patients at high risk of cardiovascular diseases and in healthy people without risk factors (Brownand Hu 2001; Kurtz et al. 2018).
In cases of smokers, diabetics, as well as patients with dyslipidemia and hypertension, vitamin C may be a substance that can improve the endothelium-dependent response (Engler et al. 2003; Sabri et al. 2016). By capturing superoxide, it increases the bioavailability of NO, inhibits lipid peroxidation, activation of platelets and neutrophils, and activation of adhesion molecules, which in turn inhibits endotheliocyte damage (Matsumoto et al. 2003). Vitamin C captures RF produced by peroxidase and inhibits the oxidation of LDL mediated by myeloper-oxidase/H2O2/ nitrite (Cai et al. 2017).
Vitamin E also has a protective effect on the endothe-lium in smoking and hypercholesterolemia (Engler et al. 2003), but its effect on diabetes remains controversial (Skyrme-Jones et al 2000; Economides et al. 2005). Vitamin E acts as a fat-soluble antioxidant, trapping hy-droperoxyl radicals in the lipid environment (Traberand Stevens 2011). The intake of antioxidants (vitamin E, C) contributes to the correction of endothelial function and
inhibits the thickening of the intima of the carotid artery (Frey et al. 2009).
N-acetylcysteine is an interchangeable amino acid that is mainly used in the treatment of cough. However, in the course of experimental studies, it turned out that it has pronounced antioxidant properties, acting on the production of glutathione, which protects the cardiovascular system from the harmful effects of TNF-a, which causes the production of ROS using NADPH oxidase and ceramide (Adamy et al. 2005; Scioli et al. 2014). Its action has a positive effect on the vessels in relation to the endothe-lium-dependent response, and it does not matter whether they were affected by atherosclerosis (Andrews et al. 2001). The effect of N-acetylcysteine on ED is mediated not only through a decrease in NADPH oxidase expression and suppression of leukocyte adhesion and secretion of inflammatory cytokines (Scioli et al. 2014), but also by inhibition of Willebrand factor-dependent platelet aggregation and collagen binding in human plasma and in mice (Chen et al. 2011), attenuation of expression matrix metallopeptidase in microvascular endothelial cells in rats (Bourraindeloup et al. 2004), as well as inhibition of cave-olin-1 activation and improvement of endothelial barrier function in mice (Beauchesne et al. 2010). These multiple effects contribute to the pronounced endotheloiprotective action of N-acetylcysteine. N-acetylcysteine interacts with endogenous and exogenous vasodilators. Moreover, in patients with systemic atherosclerosis, N-acetylcyste-ine causes vasodilation (Salsano et al. 2005) and in hypertensive patients it potentiates the hypotensive effects of angiotensin converting enzyme (ACE) inhibitors, thereby exhibiting vasoactive properties (Barrios et al. 2002).
Genistein (soy phytoestrogen) weakens ED in rats with hypertension and rats with hyperhomocysteinemia. This is achieved by increasing the activity and expression of eNOS and reducing the production of cytokines and ROS (Cho et al. 2011; Zhen et al. 2012). Genistein also increases the concentration of nitrites/nitrates and reduces the levels of ET-1 in plasma (Ou et al. 2003). Thus, genistein may be effective for the treatment of ED that occurs in connection with atherosclerosis and hypertension.
Resveratrol (phytoestrogen with antioxidant properties) is a promising multi-purpose therapeutic agent for the correction of endothelial dysfunction. According to experimental studies, resveratrol modulates several processes associated with endothelial dysfunction, such as vasorelaxation disorders, eNOS dissociation, oxidative stress, leukocyte adhesion, endothelial aging, smooth muscle proliferation and vascular remodeling (Gumanova et al. 2007; Kochkarov et al. 2008; Gureev et al. 2010). It was found that the endothelial protective effects of res-veratrol are mediated by numerous molecular targets (Li et al. 2019; Parsamanesh et al. 2021) (for example, sirtu-in-1 (SIRT1), 5' AMP-activated protein kinase (AMPK), endothelial nitric oxide synthase (eNOS), redox-sensitive transcription factor of nuclear erythroid origin (Nrf2), the receptor activated by the proliferator peroxisome (PPAR), Kruppel-like factor-2 (KLF2) and nuclear factor
"kappa-bi" (NF-kB)). Considering the fact that resvera-trol is contained in large quantities in grapes and red wine, one of the promising directions may be an enotherapeutic approach to the correction of endothelial dysfunction.
It has been experimentally revealed that ACE inhibitors exhibit endothelioprotective properties when administered to animals with heart failure (Varin et al. 2000) and those suffering from coronary heart disease (Bots et al. 2007). This effect is associated with both a decrease in the level of angiotensin II and an increase in tissue bradykinin. In addition, in animal experiments, ACE inhibitors have been shown to enhance eNOS expression (Bachetti et al. 2001; Fujii et al. 2002). This effect is realized through bradykinin B2 receptors (Bachetti et al. 2001; Fujii et al. 2002). ACE inhibitors, as well as AT1 blockers, suppress the production of ROS and vasoconstrictors derived from COX-2, which contributes to the endothelioprotective effect of these drugs (Ancion et al. 2019).
There is also evidence that some beta-blockers have an endothelial protective effect. Nebivolol, a ß1-antagonist with the property of a ß2,3-agonist, improves endotheli-um-dependent vasodilator reactions in patients with hypertension (Zepeda et al. 2012) and in smokers (Vyssoulis et al. 2004). Nebivolol also reduces vascular remodeling and expression of ET-1 and cytokines when modeling pulmonary hypertension in rats (Perros et al. 2015). Nebivo-lol increases the release of NO and reduces prothrombotic levels of fibrinogen, homocysteine and plasminogen-1 activator inhibitor in the blood, thereby affecting the state of the endothelium, in particular in smokers (Vyssoulis et al. 2004; Zepeda et al. 2012). Carvedilol, which is a non-selective antagonist of ß1 and ß2 with the property of an a-antagonist, also has a positive effect on the endothe-lium in patients with hypertension, but this effect in this case is explained by its antioxidant properties (Zepeda et al. 2012). The combined use of carvedilol with an ACE inhibitor has the most beneficial effect on the endothe-lium-dependent response in patients with hypertension and obesity (Kelly et al. 2012). Thus, this group of drugs alone and in combination with some others is suitable for the treatment of ED associated with hypertension, atherosclerosis and possibly diabetes.
Calcium channel blockers of the dihydropyridine series nicardipine and nifedipine protect endothelial cells from damage caused by exposure to ROS (Velena et al. 2016). Benidipine has an endothelial protective effect against oxLDL-induced generation of ROS in human en-dothelial cells (Matsubaraand Hasegawa 2005). Israpidin improves endothelial function in rabbits treated with cholesterol (Habib et al. 1986). Thus, the endothelioprotective effect of dihydropyridine calcium channel blockers is realized through their antioxidant effect, namely through the reduction of lipid peroxidation and the associated generation of ROS (Velena et al. 2016). In addition, am-lodipine, azelnidipine and nifedipine have been shown to have an anti-inflammatory effect, which was manifested by a decrease in the level of CRP and interleukin-6 and activation of leukocytes (Fukao et al. 2011; Yasu et al.
2013). Amlodipine in combination with a renin inhibitor improves ED in patients with arterial hypertension, which is apparently due to its NO-releasing effect and anti-inflammatory effect (Fukutomi et al. 2014; He et al. 2014; Celik et al. 2015). In addition, amlodipine in combination with statin has a better effect on the endothelium than the separate use of such drugs in rats with hypertension or diabetes (Okamura et al. 2014; Zhou et al. 2014). Thus, dihydropyridines can be effectively used not only for hypertension, but also for the treatment of ED in diabetes.
Phosphodiesterase-5 inhibitors are of therapeutic interest. Phosphodiesterase-5 (PDE5) is an enzyme that is found in vascular smooth muscles, heart, skeletal muscles, platelets, placenta, brain, kidneys, liver, pancreas, gastrointestinal tract, and lung tissues (Kass et al. 2007). In the vascular network, the main action of PDE5 is the degradation of cGMP and, thus, the induction of vasoconstriction. PDE5 inhibitors are often used to correct erectile dysfunction; they block the degradation of cGMP, which leads to its accumulation in tissues and, consequently, vasodilation (Boolell et al. 1996). PDE5 inhibitors increase the expression of eNOS and thereby increase the release of NO (Salloum et al. 2003; DeYoung et al. 2008), which contributes to a long-term vasodilator effect. PDE5 inhibitors have a number of other properties - in a model of mouse hind limb ischemia, the use of sildenafil has shown that not only does it improve blood flow restoration, but also increases capillary density and mobilization of endothelial progenitor cells (Dussault et al. 2009). In patients with vascular erectile dysfunction, daily use of vardenafil reduces arterial stiffness (Aversa et al. 2012). However, PDE5 inhibitors can be used not only in ED conditions for erectile dysfunction. For example, inhibition of PDE5 suppresses platelet activation in patients with coronary artery disease (Halcox et al. 2002) or with chronic heart failure (Bocchi et al. 2002; Lewis et al. 2007). Moreover, under conditions of modeling diabetes mellitus in rats, this group of drugs improves endothelium-dependent vasore-laxation (Schäfer et al. 2008). PDE5 inhibitors also reduce the concentration of ET-1 in plasma (Proietti et al. 2007), thereby improving microcirculation (Rosato et al. 2009). However, the mechanism underlying the action of PDE5 inhibitors to reduce ET-1 has not yet been determined.
The endothelioprotective effect of statins involves several mechanisms. Under the influence of statins, there is a decrease in LDL, which, like oxLDL, reduce the expression of eNOS (Martínez-González et al. 2001) and increase the levels of caveolin-1 (Feron et al. 2001). Statins also have a direct antioxidant effect on LDL, reducing the electronegative form of LDL (Sánchez-Quesada et al. 1999). Statins increase the bioavailability of NO by activating eNOS via the PI3K/Akt signaling pathway (Kureishi et al. 2000), stimulated by the agonists of the eNOS-hsp90 interaction (Feron et al. 2001) and the BH4-mediated binding of eNOS. These properties were demonstrated not only in the model of insulin-resistant diabetes mellitus in rats (Okamura et al. 2014), but also in patients suffering from atherosclerosis (Antoniades et al. 2011). These stud-
ies have shown that atorvastatin promotes an increase in the vascular content of BH4 and bioavailability of NO, as well as a decrease in ROS production by stimulating the expression and activity of the GTP cyclohydrolase I gene (Antoniades et al. 2011). In addition, statins increase eNOS expression by increasing the stability of eNOS mRNA (Rikitake and Liao 2005; Kosmidou et al. 2007). Statins also have an anti-inflammatory effect (Antonopou-los et al. 2012; Denisyuk 2015). For example, treatment with atorvastatin reduces pro-inflammatory cytokines (TNF-a, interleukin-1 and -6), intercellular adhesion molecules and CRP levels in the blood of patients with hypercholesterolemia (Ascer et al. 2004), while rapid discontinuation of statins provokes an increase in proinflammatory and prothrombotic markers (Lai et al. 2005). Statins have also been shown to increase the number of circulating endothelial progenitor cells, probably via the PI3K/Akt pathway (Dimmeler et al. 2001), which may contribute to their longer-term endotheliotropic effect.
One of the promising therapeutic agents in the treatment of ED is Angiotensin-(1-7). It is a metabolite of angiotensin I (Su 2014). It can also be obtained from angiotensin II under the action of prolylcarboxypeptidase (Mallela et al. 2008) and carboxypeptidase APF2 (Zisman et al. 2003). Angiotensin-(1-7) activates endothelial cell eNOS via the PI3K/Akt pathway, thereby inhibiting angiotensin II-in-duced activation of NADPH oxidase (Sampaio et al. 2007a, 2007b). Long-term angiotensin therapy (1-7) improves renal ED, pathogenetically caused by apolipoprotein E deficiency (Stegbauer et al. 2011) and diet-induced obesity in mice (Beyer et al. 2013) which is probably mediated by an increase in NO release (Traskand Ferrario 2007)and eNOS expression (Zhang et al. 2008;Costaetal. 2010). Angioten-sin-(1-7) has also been shown to disrupt the production of ROS activated by the angiotensin receptor AT1 NADPH oxidases in the modeling of hypertension or diabetes melli-tus in rats (Fraga-Silva et al. 2013; Pernomian et al. 2014). Angiotensin-(1-7) restores the production and migration of NO/cGMP, and increases the stability and proliferative activity of endothelial progenitor cells in patients with diabetes (Jarajapu et al. 2013). However, there is not enough information about the effectiveness of angiotensin-(1-7) in relation to the human endothelium yet.
In recent years, a new class of drug has appeared - a direct renin inhibitor - aliskiren. The endothelioprotective effect of aliskiren on animals has been experimentally demonstrated. In rabbits with Watanabe hyperlipidemia, the renin inhibitor aliskiren enhanced both an increase in NO concentration in the blood and a decrease in NO release after treatment with L-NMMA (nitric oxide syn-thase inhibitor) to a degree similar to that obtained with valsartan; in addition, it simultaneously reduced the area of plaques in the aorta. These effects were further enhanced by the combined use of aliskiren and valsartan (Imanishi et al. 2008), which indicates that the effect of aliskiren is independent of angiotensin II. However, information on the effect of aliskiren on the functional activity of the human endothelium remains insufficient.
Endogenous bradykinin, as mentioned above, has many different effects on the functional state of the endothelium. As a pharmacological drug, it protects against the death of microvascular endothelial cells caused by ROS and toxins (Bovenzi et al. 2010). Long-term use of bradykinin preserves the expression of eNOS in dogs with cardiac insufficiency caused by pacing (Tonduangu et al. 2004), and also enhances the expression of eNOS and nNOS (neuronal nitric oxide synthase) in the vessels and heart of dogs with cardiomyopathy with dystrophin deficiency (Dabire et al. 2012; Su et al. 2012). However, due to the very short half-life and the involvement of bradykinin in inflammation (Chen et al. 2004) and cancer (Montana and Sontheimer 2011; Yu et al. 2013), the use of bradykinin in clinical practice remains problematic.
The eNOS transcription enhancer, the chemical compound AVE3085, has certain prospects for ED correction. It stimulates eNOS expression, suppresses oxidative stress and activates platelets, thereby improving endo-thelium-dependent relaxation and heart function, which was found in animals with various experimental diseases (Cheang et al. 2011; Yang et al. 2011). This compound, among other things, prevents the inhibition of vasodi-lation by asymmetric dimethylarginine (ADMA) in the rings of the human internal thoracic artery and in the rings of the porcine coronary artery (Xuan et al. 2012; Xue et al. 2012). However, its clinical efficacy in relation to humans has not yet been demonstrated.
One of the controversial candidates for the treatment of ED is the if-channel inhibitor ivabradine. A number of studies have revealed its positive effect on both endotheli-um-dependent vasodilation and eNOS expression in both animals and humans (Bolduc et al. 2011; Musikhina et al. 2012; Orea-Tejeda et al. 2013). However, the authors of some studies note that there was no significant improvement in vasodilation under the action of ivabradine in patients with microvascular angina (Villano et al. 2013), as well as in patients with other forms of coronary heart disease (Jochmann et al. 2014) or type II diabetes (Nerla et al. 2012). Moreover, the use of ivabaradine (in addition to standard treatment) in patients with stable coronary artery disease without heart failure was associated with an increase in the frequency of atrial fibrillation, which casts doubt on the usefulness of this drug (Fox et al. 2014).
There is evidence of endothelioprotective effects of sphingosine-1-phosphate (C1F). C1F is a signaling sphin-golipid formed by sphingosine kinase in blood and tissues, regulates vascular proliferation, their permeability and transport of T- and B-lymphocytes. C1P enhances the function of the endothelial barrier (Wilkerson et al. 2014) stimulates the release of NO through Akt-mediated phosphorylation of eNOS (Igarashi et al. 2001) and restores high-density lipoproteins (Tong et al. 2014). C1F also has anti-inflammatory properties and has a protective effect against lung damage caused by endotoxins (Lucke and Levkau 2010). Moreover, C1F has a strong effect on the differentiation of adipose tissue-derived stem cells into endothelial-like cells and the activation of eNOS in
these cells (Arya et al. 2014). All these properties of C1F can contribute to its endothelioprotective effect. Interestingly, FTY720, an oral active analogue of C1F, also demonstrates similar properties (Van der Giet et al. 2008). Thus, C1F and its analogues can be used to improve the functional state of the endothelium, especially in such pathological conditions as atherosclerosis and acute lung damage, when a violation of the function of the endothe-lial barrier is manifested (Natarajan et al. 2013).
Another promising drug for the treatment of ED is erythropoietin (EPO). EPO activates the PI3K/Akt pathway and promotes the release of NO. In addition, there is evidence showing that the concentration of EPO in the blood is directly proportional to the amount of EPC in patients with coronary heart disease (Heeschen et al. 2003). In addition, the introduction of EPO increased the amount of EPC. These data suggest that EPO may play an important role in the production of EPC.
Several authors of large prospective studies report a significant reduction in cardiovascular events in patients with major depressive disorder who responded to antide-pressant therapy (Santangelo et al. 2009; Kimmel et al. 2011; Safronenko et al. 2021). In these patients, there was a significant improvement in ED and inflammation markers, which was confirmed by increased endothelial dilation and a decrease in interleukin-6 levels (Pizzi et al. 2009; Tseng et al. 2010; Lopez-Vilchez et al. 2016). There is also evidence to suggest that the effectiveness of low doses of lithium in borderline personality disorder and stroke may be partially associated with improved endothelial function and reduced inflammation in the endothelium (Lyoo et al. 2010; Mohammadianinejad et al. 2014; Li et al. 2018).
Recently, H2S donors have attracted the attention of researchers with the discovery of new therapeutic strategies for the treatment of various ED-related diseases (Benavides et al. 2007; Martelli et al. 2014; Tomasova et al. 2015; Abramavicius et al. 2021). Sodium thiosulfate is the main product of H2S oxidation. Sodium thiosulfate is an odorless inorganic water-soluble compound with the chemical formula Na2S2O3 and a molecular weight of 158.11 g/mol. Recent data show that sodium thiosulfate has antioxidant, anti-inflammatory and antihypertensive properties (Roorda et al. 2021), which makes it a potential candidate molecule in the treatment of ED-related diseases. In addition to the fact that thiosulfate is a stable non-toxic metabolite of H2S (Bilska-Wilkosz et al. 2017), it is also sulfane sulfur, which is defined as sulfur atoms covalently bound to other sulfur atoms, which makes it unstable and easily oxidized in air and reduced by thiols (Koike and Ogasawara 2016). It is known that compounds containing sulfane sulfur have cell regulation effects due to activation or inactivation of enzymes and changes in protein activity (Mustafa et al. 2009). The functions of sulfane sulfur include antioxidant regulation, sulfonation of tRNA and the formation of iron-sulfur protein. As an H2S donor molecule, sodium thiosulfate has unexplored therapeutic potential in the context of many diseases.
Over the past few years, a number of independent research groups have found positive effects of sodium thiosulfate on animal models of various diseases (Zhang et al. 2021).
Conclusion
ED is a typical pathological process involved in the patho-genesis of many diseases. In some diseases, such as atherosclerosis, ED plays a crucial role in the development of pathology, whereas in others, such as hypertension and type II diabetes mellitus, ED usually occurs as a complication, but subsequently contributes to the development and progression of organ damage. It is obvious that multiple mechanisms are involved in the pathogenesis of ED development, such as inflammation, increased ROS and RFA, cellular apoptosis, increased production of vasocon-
strictors, decreased production of vasodilators and vascular remodeling, and each specific pathology may include them to a greater or lesser extent. However, a decrease in the bioavailability of NO seems to play a crucial role. Thus, pharmacological agents with endothelioprotective properties can provide more therapeutic benefits than a drug without such an effect. Considering the important role of ED in the development and progression of many diseases, it is becoming increasingly attractive to consider ED as a primary therapeutic goal. For this reason, the evaluation of the endothelioprotective effect is becoming increasingly attractive in the development of new drugs.
Conflict of interest
The authors declare that there is no conflict of interests.
References
■ Abramavicius S, Petersen AG, Renaltan NS, Prat-Duran J, Tor-regrossa R, Stankevicius E, Whiteman M, Simonsen U (2021) GYY4137 and sodium hydrogen sulfide relaxations are inhibited by L-cysteine and KV7 channel blockers in rat small mesenteric rteries. Frontiers in Pharmacology 26(12): 613989. https://doi.org/10.3389/ fphar.2021.613989 [PubMed]
■ Adamy C, Le Corvoisier P, Candiani G, Kirsch M, Pavoine C, Defer N, Bourin MC, Su JB, Vermes E, Hittinger L, Pecker E (2005) Tumor necrosis factor alpha and glutathione interplay in chronic heart failure. Archives des Maladies du Coeur et des Vaisseaux 98: 906-912. [PubMed]
■ Afonasyeva TN (2019) Endothelial dysfunction. Possibilities for early diagnosis. Health and Education in the XXI Century 64(1): 34-41. https://doi.org/10.18821/0869-2084-2019-64-1-34-41
■ Ancion A, Tridetti J, Nguyen Trung ML, Oury C, Lancellotti P (2019) A review of the role of bradykinin and nitric oxide in the cardioprotective action of angiotensin-converting enzyme inhibitors: Focus on perindopril. Cardiology and Therapy 8(2): 179-191. https://doi.org/10.1007/s40119-019-00150-w [PMC] [PubMed]
■ Andrews NP, Prasad A, Quyyumi AA (2001) N-acetylcysteine improves coronary and peripheral vascular function. Journal of the American College of Cardiology 37: 117-123. https://doi. org/10.1016/S0735-1097(00)01093-7 [PubMed]
■ Antoniades C, Bakogiannis C, Leeson P, Guzik TJ, Zhang MH, Tou-soulis D, Antonopoulos AS, Demosthenous M, Marinou K, Hale A, Paschalis A, Psarros C, Triantafyllou C, Bendall J, Casadei B, Stefa-nadis C, Channon KM (2011) Rapid, direct effects of statin treatment on arterial redox state and nitric oxide bioavailability in human atherosclerosis via tetrahydrobiopterin-mediated endothelial nitric oxide synthase coupling. Circulation 124: 335-345. https://doi. org/10.1161/CIRCULATIONAHA.110.985150 [PMC] [PubMed]
■ Antoniades C, Shirodaria C, Leeson P, Antonopoulos A, Warrick N, Van-Assche T, Cunnington C, Tousoulis D, Pillai R, Ratnatunga C, Stefanadis C, Channon KM (2009) Association of plasma asymmetrical dimethylarginine (ADMA) with elevated vascular superoxide production and endothelial nitric oxide synthase uncoupling: implications for endothelial function in human atherosclerosis. European Heart Journal 30: 1142-1150. https://doi.org/10.1093/eurheartj/ehp061 [PubMed]
■ Antonopoulos AS, Margaritis M, Lee R, Channon K, Antoniades C (2012) Statins as anti-inflammatory agents in atherogenesis: molecular mechanisms and lessons from the recent clinical trials. Current Pharmaceutical Design 18: 1519-1530. https://doi. org/10.2174/138161212799504803 [PMC] [PubMed]
■ Arya D, Chang S, DiMuzio P, Carpenter J, Tulenko TN (2014) Sphin-gosine-1-phosphate promotes the differentiation of adipose-derived stem cells into endothelial nitric oxide synthase (eNOS) expressing endothelial-like cells. Journal of Biomedical Science 21(1): 55. https://doi.org/10.1186/1423-0127-21-55 [PMC] [PubMed]
■ Ascer E, Bertolami MC, Venturinelli ML, Buccheri V, Souza J, Nicolau JC, Ramires JA, Serrano CV (2004) Atorvastatin reduces proinflammatory markers in hypercholesterolemic patients. Atherosclerosis 177(1): 161-166. https://doi.org/10.1016Zj.atherosclero-sis.2004.07.003 [PubMed]
■ Aversa A, Letizia C, Francomano D, Bruzziches R, Natali M, Lenzi A (2012) A spontaneous, double-blind, double-dummy cross-over study on the effects of daily vardenafil on arterial stiffness in patients with vasculogenic erectile dysfunction. International Journal of Cardiology 160(3): 187-191. https://doi.org/10.1016/j.ij-card.2011.04.003 [PubMed]
■ Avogaro A, de Kreutzenberg SV, Fadini G (2008) Endothelial dysfunction: causes and consequences in patients with diabetes mellitus. Diabetes Research and Clinical Practice 82(Suppl 2): S94. https:// doi.org/10.1016/j.diabres.2008.09.021 [PubMed]
■ Bachetti T, Comini L, Pasini E, Cargnoni A, Curello S, Ferrari R (2001) Ace-inhibition with quinapril modulates the nitric oxide pathway in normotensive rats. Journal of Molecular and Cellular Cardiology 33(3): 395-403. https://doi.org/10.1006/jmcc.2000.1311 [PubMed]
■ Badimon L, Romero JC, Cubedo J, Borrell-Pages M (2012) Circulating biomarkers. Thrombosis Research 130(Suppl 1): S12-S15. https://doi.org/10.1016/j.thromres.2012.08.262 [PubMed]
■ Barbieri SS, Zacchi E, Amadio P, Gianellini S, Mussoni L, Weksler BB, Tremoli E (2011) Cytokines present in smokers' serum interact with smoke components to enhance endothelial dysfunction. Cardiovascular Research 90(3): 475-483. https://doi.org/10.1093/ cvr/cvr032 [PubMed]
■ Barrios V, Calderón A, Navarro-Cid J, Lahera V, Ruilope LM (2002) N-acetylcysteine potentiates the antihypertensive effect of ACE inhibitors in hypertensive patients. Blood Pressure 11(4): 235-239. https://doi.org/10.1080/08037050213760 [PubMed]
■ Barton M (2013) Prevention and endothelial therapy of coronary artery disease. Current Opinion in Pharmacology 13(2): 226-241. https://doi.org/10.1016/j.coph.2013.05.005 [PubMed]
■ Baszczuk A, Kopczyñski Z, Thielemann A (2014) Endothelial dysfunction in patients with primary hypertension and hyperhomocys-teinemia. Postepy Higieny i Medycyny Doswiadczalnej 68: 91-100. https://doi.org/10.5604/17322693.1087521 [PubMed] [In Polish]
■ Beauchesne E, Desjardins P, Butterworth RF, Hazell AS (2010) Up-regulation of caveolin-1 and blood-brain barrier breakdown are attenuated by N-acetylcysteine in thiamine deficiency. Neurochem-istry International 57(7): 830-837. https://doi.org/10.1016Zj.neu-int.2010.08.022 [PubMed]
■ Belenichev IF, Gorchakov NO, Samura IB, Savchenko NV, Bukh-tiyarova NV, Popazova OO (2021) Pharmacological properties of selenium and its preparations: from antioxidant to neuroprotec-tor. Research Results in Pharmacology 7(4): 29-40. https://doi. org/10.3897/rrpharmacology.7.73051
■ Benavides GA, Squadrito GL, Mills RW, Patel HD, Isbell TS, Patel RP, Darley-Usmar VM, Doeller JE, Kraus DW (2007) Hydrogen sulfide mediates the vasoactivity of garlic. Proceedings of the National Academy of Sciences of the United States of America 104(46): 17977-17982. https://doi.org/10.1073/pnas.0705710104 [PubMed]
■ Beyer AM, Guo DF, Rahmouni K (2013) Prolonged treatment with angiotensin 1-7 improves endothelial function in diet-induced obesity. Journal of Hypertension 31(4): 730-738. https://doi.org/10.1097/ HJH.0b013e32835ecbe5 [PMC] [PubMed]
■ Bilska-Wilkosz A, Iciek M, Górny M, Kowalczyk-Pachel D (2017) The role of hemoproteins: Hemoglobin, myoglobin and neuroglo-bin in endogenous thiosulfate production processes. International Journal of Molecular Sciences 18(6): 1315. https://doi.org/10.3390/ ijms18061315 [PMC] [PubMed]
■ Blake GJ, Ridker PM (2001) Novel clinical markers of vascular wall inflammation. Circulation Research 89(9): 763-771. https://doi. org/10.1161/hh2101.099270 [PubMed]
■ Bocchi EA, Guimarâes G, Mocelin A, Bacal F, Bellotti G, Ramires JF (2002) Sildenafil effects on exercise, neurohormonal activation, and erectile dysfunction in congestive heart failure: a double-blind, placebo-controlled, randomized study followed by a prospective treatment for erectile dysfunction. Circulation 106(9): 1097-1103. https://doi.org/10.1161/01.CIR.0000027149.83473.B6 [PubMed]
■ Boegehold MA (2013) The effect of high salt intake on endotheli-al function: reduced vascular nitric oxide in the absence of hypertension. Journal of Vascular Research 50(6): 458-467. https://doi. org/10.1159/000355270 [PubMed]
■ Boger RH, Lentz SR, Bode-Boger SM, Knapp HR, Haynes WG (2001) Elevation of asymmetrical dimethylarginine may mediate endothelial dysfunction during experimental hyperhomocyst(e) inaemia in humans. Clinical Science 100(2): 161-167. https://doi. org/10.1042/cs1000161 [PubMed]
■ Bolduc V, Drouin A, Gillis MA, Duquette N, Thorin-Trescases N, Frayne-Robillard I, Des Rosiers C, Tardif JC, Thorin E (2011) Heart rate-associated mechanical stress impairs carotid but not cerebral artery compliance in dyslipidemic atherosclerotic mice. American Journal of Physiology. Heart and Circulatory Physiology 301(5):
H2081-H2092. https://doi.org/10.1152/ajpheart.00706.2011 [PMC] [PubMed]
■ Bonetti PO, Lerman LO, Lerman A (2003) Endothelial dysfunction: a marker of atherosclerotic risk. Arteriosclerosis, Thrombosis, and Vascular Biology 23(2): 168-175. https://doi.org/10.1161/01. ATV.0000051384.43104.FC [PubMed]
■ Boolell M, Allen MJ, Ballard SA, Gepi-Attee S, Muirhead GJ, Nay-lor AM, Osterloh IH, Gingell C (1996) Sildenafil: an orally active type 5 cyclic GMP-specific phosphodiesterase inhibitor for the treatment of penile erectile dysfunction. International Journal of Impotence Research 8(2): 47-52. [PubMed]
■ Bots ML, Remme WJ, Luscher TF, Fox KM, Bertrand M, Ferrari R, Simoons ML, Grobbee DE, EUROPA-PERFECT Investigators (2007) ACE inhibition and endothelial function: main findings of PERFECT, a sub-study of the EUROPA trial. Cardiovascular Drugs and Therapy 21(4): 269-279. https://doi.org/10.1007/s10557-007-6041-3 [PMC] [PubMed]
■ Bourraindeloup M, Adamy C, Candiani G, Cailleret M, Bourin MC, Badoual T, Su JB, Adubeiro S, Roudot-Thoraval F, Dubois-Rande JL, Hittinger L, Pecker F (2004) N-acetylcysteine treatment normalizes serum tumor necrosis factor-alpha level and hinders the progression of cardiac injury in hypertensive rats. Circulation 110(14): 2003-2009. https://doi.org/10.1161/01.CIR.0000143630.14515.7C [PubMed]
■ Bovenzi V, Savard M, Morin J, Cuerrier CM, Grandbois M, Gobeil F (2010) Bradykinin protects against brain microvascular endothelial cell death induced by pathophysiological stimuli. Journal of Cellular Physiology 222 (1):168-176. https://doi.org/10.1002/jcp.21933 [PubMed]
■ Brown AA, Hu FB (2001) Dietary modulation of endothelial function: implications for cardiovascular disease. The American Journal of Clinical Nutrition 73(4): 673-686. https://doi.org/10.1093/ ajcn/73.4.673 [PubMed]
■ Brown NK, Zhou Z, Zhang J, Zeng R, Wu J, Eitzman DT, Chen YE, Chang L (2014) Perivascular adipose tissue in vascular function and disease: a review of current research and animal models. Arteriosclerosis, Thrombosis, and Vascular Biology 34(8): 1621-1630. https:// doi.org/10.1161/ATVBAHA.114.303029 [PMC] [PubMed]
■ Bucci M, Papapetropoulos A, Vellecco V, Zhou Z, Pyriochou A, Roussos C, Roviezzo F, Brancaleone V, Cirino G (2010) Hydrogen sulfide is an endogenous inhibitor of phosphodiesterase activity. Arteriosclerosis, Thrombosis, and Vascular Biology 30(10): 1998-2004. https://doi.org/10.1161/ATVBAHA.110.209783 [PubMed]
■ Busse R, Fleming I (2006) Vascular endothelium and blood flow. Handbook of Experimental Pharmacology 176(Pt 2): 43-78. https:// doi.org/10.1007/3-540-36028-X_2 [PubMed]
■ Cai W, Duan X-M, Liu Y, Yu J, Tang Y-L, Liu Z-L, Jiang S, Zhang C-P, Liu J-Y, Xu J-X (2017) Uric acid induces endothelial dysfunction by activating the HMGB1/RAGE signaling pathway. BioMed Research International 2017: 4391920. https://doi.org/10.1155/2017/4391920 [PMC] [PubMed]
■ Celik T, Balta S, Karaman M, Ahmet Ay S, Demirkol S, Ozturk C, Dinc M, Unal HU, Yilmaz MI, Kilic S, Kurt G, Tas A, Iyisoy A, Quarti-Trevano F, Fici F, Grassi G (2015) Endocan, a novel marker of endothelial dysfunction in patients with essential hypertension: comparative effects of amlodipine and valsartan. Blood Pressure 24(1): 55-60. https://doi.org/10.3109/08037051.2014.972816 [PubMed]
■ Champagne S, Hittinger L, Heloire F, Suto Y, Sambin L, Crozatier B, Su JB (2002) Reduced coronary vasodilator responses to amlodipine in pacing-induced heart failure in conscious dogs: role of nitric oxide. British Journal of Pharmacology 136(2): 264-270. https://doi. org/10.1038/sj.bjp.0704701 [PMC] [PubMed]
■ Cheang WS, Wong WT, Tian XY, Yang Q, Lee HK, He GW, Yao X, Huang Y (2011) Endothelial nitric oxide synthase enhancer reduces oxidative stress and restores endothelial function in db/db mice. Cardiovascular Research 92(2): 267-275. https://doi.org/10.1093/cvr/ cvr233 [PubMed]
■ Chen BC, Yu CC, Lei HC, Chang MS, Hsu MJ, Huang CL, Chen MC, Sheu JR, Chen TF, Chen TL, Inoue H, Lin CH (2004) Bradykinin B2 receptor mediates NF-kappaB activation and cyclooxygen-ase-2 expression via the Ras/Raf-1/ERK pathway in human airway epithelial cells. The Journal of Immunology 173(8): 5219-5228. https://doi.org/10.4049/jimmunol.173.8.5219 [PubMed]
■ Chen CA, Wang TY, Varadharaj S, Reyes LA, Hemann C, Talukder MA, Chen YR, Druhan LJ, Zweier JL (2010) S-glutathionylation uncouples eNOS and regulates its cellular and vascular function. Nature 468(7327): 1115-1118. https://doi.org/10.1038/nature09599 [PMC] [PubMed]
■ Chen J, Reheman A, Gushiken FC, Nolasco L, Fu X, Moake JL, Ni H, Lopez JA (2011) N-acetylcysteine reduces the size and activity of von Willebrand factor in human plasma and mice. The Journal of Clinical Investigation 121(2): 593-603. https://doi.org/10.1172/ JCI41062 [PMC] [PubMed]
■ Chen S, Shen Y, Liu YH, Dai Y, Wu ZM, Wang XQ, Yang CD, Li LY, Liu JM, Zhang LP, Shen WF, Ji R, Lu L, Ding FH (2021) Impact of glycemic control on the association of endothelial dysfunction and coronary artery disease in patients with type 2 diabetes mellitus. Cardiovascular Diabetology 20(1): 64. https://doi.org/10.1186/s12933-021-01257-y [PMC] [PubMed]
■ Cheng Z, Jiang X, Kruger WD, Pratico D, Gupta S, Mallilankara-man K, Madesh M, Schafer AI, Durante W, Yang X, Wang H (2011) Hyperhomocysteinemia impairs endothelium-derived hyperpolar-izing factor-mediated vasorelaxation in transgenic cystathionine beta synthase-deficient mice. Blood 118(7): 1998-2006. https://doi. org/10.1182/blood-2011-01-333310 [PMC] [PubMed]
■ Cheng Z, Yang X, Wang H (2009) Hyperhomocysteinemia and endo-thelial dysfunction. Current Hypertension Reviews 5(2): 158-165. https://doi.org/10.2174/157340209788166940 [PMC] [PubMed]
■ Ching C, Gustafson D, Thavendiranathan P, Fish JE (2021) Cancer therapy-related cardiac dysfunction: is endothelial dysfunction at the heart of the matter? Clinical Science 135(12): 1487-1503. https:// doi.org/10.1042/CS20210059 [PubMed]
■ Cho HY, Park CM, Kim MJ, Chinzorig R, Cho CW, Song YS (2011) Comparative effect of genistein and daidzein on the expression of MCP-1, eNOS, and cell adhesion molecules in TNF-a-stimulated HUVECs. Nutrition Research and Practice 5 (5): 381-388. https:// doi.org/10.4162/nrp.2011.5.5.381 [PMC ] [PubMed]
■ Corda S, Laplace C, Vicaut E, Duranteau J (2001) Rapid reactive oxygen species production by mitochondria in endothelial cells exposed to tumor necrosis factor-alpha is mediated by ceramide. American Journal of Respiratory Cell and Molecular Biology 24(6): 762-768 https://doi.org/10.1165/ajrcmb.24.6.4228 [PubMed]
■ Cortese-Krott MM, Kuhnle GG, Dyson A, Fernandez BO, Grman M, DuMond JF, Barrow MP, McLeod G, Nakagawa H, Ondrias K, Nagy P, King SB, Saavedra JE, Keefer LK, Singer M, Kelm M, Butler
AR, Feelisch M (2015) Key bioactive reaction products of the NO/ H2S interaction are S/N-hybrid species, polysulfides, and nitroxyl. Proceedings of the National Academy of Sciences of the United States of America 112(34): E4651-E4660. https://doi.org/10.1073/ pnas.1509277112 [PMC] [PubMed]
■ Costa MA, Lopez Verrilli MA, Gomez KA, Nakagawa P, Peña C, Arranz C, Gironacci MM (2010) Angiotensin-(1-7) upregulates cardiac nitric oxide synthase in spontaneously hypertensive rats. American Journal of Physiology. Heart and Circulatory Physiology 299(4): H1205-H1211. https://doi.org/10.1152/ajpheart.00850.2009 [PubMed]
■ Cziráki A, Lenkey Z, Sulyok E, Szokodi I, Koller A (2020) L-Arginine-nitric oxide-asymmetric dimethylarginine pathway and the coronary circulation: Translation of basic science results to clinical practice. Frontiers in Pharmacology 11: 569914. https://doi. org/10.3389/fphar.2020.569914 [PMC] [PubMed]
■ Dabiré H, Barthélémy I, Blanchard-Gutton N, Sambin L, Sampe-drano CC, Gouni V, Unterfinger Y, Aguilar P, Thibaud JL, Ghaleh B, Bizé A, Pouchelon JL, Blot S, Berdeaux A, Hittinger L, Chetboul V, Su JB (2012) Vascular endothelial dysfunction in Duchenne muscular dystrophy is restored by bradykinin through upregulation of eNOS and nNOS. Basic Research in Cardiology 107(1): 240. https:// doi.org/10.1007/s00395-011-0240-6 [PMC] [PubMed]
■ Dambala K, Paschou SA, Michopoulos A, Siasos G, Goulis DG, Vavilis D, Tarlatzis BC (2019) Biomarkers of endothelial dysfunction in women with polycystic ovary syndrome. Angiology 70(9): 797-801. https://doi.org/10.1177/0003319719840091 [PubMed]
■ Damy T, Ratajczak P, Shah AM, Camors E, Marty I, Hasenfuss G, Marotte F, Samuel JL, Heymes C (2004) Increased neuronal nitric oxide synthase-derived NO production in the failing human heart. Lancet 363(9418): 1365-1367. https://doi.org/10.1016/S0140-6736(04)16048-0 [PubMed]
■ Darblade B, Caillaud D, Poirot M, Fouque M, Thiers JC, Rami J, Bayard F, Arnal JF (2001) Alteration of plasmalemmal caveolae mimics endothelial dysfunction observed in atheromatous rabbit aorta. Cardiovascular Research 50(3): 566-576. https://doi.org/10.1016/ S0008-6363(01)00251-6 [PubMed]
■ Davenport AP, Hyndman KA, Dhaun N, Southan C, Kohan DE, Pollock JS, Pollock DM, Webb DJ, Maguire JJ (2016) Endothelin. Pharmacological Reviews 68(2): 357-418. https://doi.org/10.1124/ pr.115.011833 [PMC] [PubMed]
■ de Lemos JA, Hennekens CH, Ridker PM (2000) Plasma concentration of soluble vascular cell adhesion molecule-1 and subsequent cardiovascular risk. Journal of the American College of Cardiology 36(2): 423-426. https://doi.org/10.1016/S0735-1097(00)00742-7 [PubMed]
■ De Young LX, Domes T, Lim K, Carson J, Brock GB (2008) Endothelial rehabilitation: the impact of chronic PDE5 inhibitors on erectile function and protein alterations in cavernous tissue of diabetic rats. European Urology 54(1): 213-220. https://doi.org/10.1016/j. eururo.2008.02.034 [PubMed]
■ Denisyuk TA (2015) Endothelio-and cardioprotective effects of HMG-Co-reductase in endotoksin-inducing endotelial dysfunction. Research Result. Medicine and Pharmacy Series 1(4): 56-61. https:// doi.org/10.18413/2313-8955-2015-1-4-56-61 [in Russian]
■ Delgado GE, Krämer BK, Siekmeier R, Yazdani B, März W, Leipe J, Kleber ME (2020) Influence of smoking and smoking cessation on biomarkers of endothelial function and their association with mortality. Atherosclerosis 292: 52-59. https://doi.org/10.1016Zj.ath-erosclerosis.2019.11.017 [PubMed]
■ Deng G, Long Y, Yu YR, Li MR (2010) Adiponectin directly improves endothelial dysfunction in obese rats through the AMPK-eNOS. International Journal of Obesity 34(1): 165-171. https://doi. org/10.1038/ijo.2009.205 [PubMed]
■ Dimmeler S, Aicher A, Vasa M, Mildner-Rihm C, Adler K, Tiemann M, Rütten H, Fichtlscherer S, Martin H, Zeiher AM (2001) HMG-CoA reductase inhibitors (statins) increase endothelial progenitor cells via the PI 3-kinase/Akt pathway. The Journal of Clinical Investigation 108(3): 391-397. https://doi.org/10.1172/JCI13152 [PMC] [PubMed]
■ Dussault S, Maingrette F, Menard C, Michaud SE, Haddad P, Gro-leau J, Turgeon J, Perez G, Rivard A (2009) Sildenafil increases en-dothelial progenitor cell function and improves ischemia-induced neovascularization in hypercholesterolemic apolipoprotein E-defi-cient mice. Hypertension 54(5): 1043-1049. https://doi.org/10.1161/ HYPERTENSIONAHA.109.139451 [PubMed]
■ Economides PA, Khaodhiar L, Caselli A, Caballero AE, Keenan H, Bursell SE, King GL, Johnstone MT, Horton ES, Veves A (2005) The effect of vitamin E on endothelial function of micro- and macrocirculation and left ventricular function in type 1 and type 2 diabetic patients. Diabetes 54(1): 204-211. https://doi.org/10.2337/diabe-tes.54.1.204 [PubMed]
■ Efimenko SV, Chernyaeva SS, Peresypkina AA, Pobeda AS, Korokin MV, Beskhmelnitsyna EA, Pokrovskii MV, Corac AM (2022) Studies to elucidate the effect and antiapoptotic mechanism of 2-eth-yl-3-hydroxy-6-methylpyridine-n-acetyltaurinate in a rat model of retinal ischemia-reperfusion. Research Results in Biomedicine 8(3): 317-326. https://doi.org/10.18413/2658-6533-2022-8-3-0-5
■ Ekeloef S, Oreskov JO, Falkenberg A, Burcharth J, Schou-Pedersen AMV, Lykkesfeldt J, Gögenur I (2020) Endothelial dysfunction and myocardial injury after major emergency abdominal surgery: a prospective cohort study. BMC Anesthesiology 20(1): 67. https://doi. org/10.1186/s12871-020-00977-0 [PMC] [PubMed]
■ Engler MM, Engler MB, Malloy MJ, Chiu EY, Schloetter MC, Paul SM, Stuehlinger M, Lin KY, Cooke JP, Morrow JD, Ridker PM, Rifai N, Miller E, Witztum JL, Mietus-Snyder M (2003) Anti-oxidant vitamins C and E improve endothelial function in children with hyperlipidemia: Endothelial assessment of risk from lipids in youth (EARLY) Trial. Circulation 108(9): 1059-1063. https://doi. org/10.1161/01.CIR.0000086345.09861.A0 [PubMed]
■ Epstein SE, Zhu J, Burnett MS, Zhou YF, Vercellotti G, Hajjar D (2000) Infection and atherosclerosis: potential roles of pathogen burden and molecular mimicry. Arteriosclerosis, Thrombosis, and Vascular Biology 20(6): 1417-1420. https://doi.org/10.1161/01. ATV.20.6.1417 [PubMed]
■ Fanelli V, Fiorentino M, Cantaluppi V (2020) Acute kidney injury in SARS-CoV-2 infected patients. Critical Care 24(1): 155. https://doi. org/10.1186/s13054-020-02872-z [PMC] [PubMed]
■ Farbstein D, Kozak-Blickstein A, Levy AP (2010) Antioxidant vitamins and their use in preventing cardiovascular disease. Molecules 15(11): 8098-8110. https://doi.org/10.3390/molecules15118098 [PMC] [PubMed]
■ Feron O, Dessy C, Desager JP, Balligand JL (2001) Hydroxy-meth-ylglutaryl-coenzyme A reductase inhibition promotes endotheli-al nitric oxide synthase activation through a decrease in caveolin abundance. Circulation 103(1): 113-118. https://doi.org/10.1161/01. CIR.103.1.113 [PubMed]
■ Fleming I, Mohamed A, Galle J, Turchanowa L, Brandes RP, Fis-slthaler B, Busse R (2005) Oxidized low-density lipoprotein in-
creases superoxide production by endothelial nitric oxide synthase by inhibiting PKCalpha. Cardiovascular Research 65(4): 897-906. https://doi.org/10.1016/j.cardiores.2004.11.003 [PubMed]
■ Förstermann U, Münzel T (2006) Endothelial nitric oxide synthase in vascular disease: from marvel to menace. Circulation 113(13): 1708-1714. https://doi.org/10.1161/CIRCULATIONA-HA.105.602532 [PubMed]
■ Fox K, Ford I, Ferrari R (2014) Ivabradine in stable coronary artery disease. The New England Journal of Medicine 371(25): 2435. https://doi.org/10.1056/NEJMc1413158 [PubMed]
■ Fraga-Silva RA, Costa-Fraga FP, Murça TM, Moraes PL, Martins Lima A, Lautner RQ, Castro CH, Soares CM, Borges CL, Nadu AP, Oliveira ML, Shenoy V, Katovich MJ, Santos RA, Raizada MK, Ferreira AJ (2013) Angiotensin-converting enzyme 2 activation improves endothelial function. Hypertension 61(6): 1233-1238. https://doi.org/10.1161/HYPERTENSIONAHA.111.00627 [PMC] [PubMed]
■ Frey RS, Ushio-Fukai M, Malik AB (2009) NADPH oxidase-depen-dent signaling in endothelial cells: role in physiology and pathophys-iology. Antioxidants and Redox Signaling 11(4): 791-810. https:// doi.org/10.1089/ars.2008.2220 [PMC] [PubMed]
■ Fujii M, Wada A, Tsutamoto T, Ohnishi M, Isono T, Kinoshita M (2002) Bradykinin improves left ventricular diastolic function under long-term angiotensin-converting enzyme inhibition in heart failure. Hypertension 39(5): 952-957. https://doi.org/10.1161/01. HYP.0000015613.78314.9E [PubMed]
■ Fukao K, Shimada K, Hiki M, Kiyanagi T, Hirose K, Kume A, Ohsa-ka H, Matsumori R, Kurata T, Miyazaki T, Daida H (2011) Effects of calcium channel blockers on glucose tolerance, inflammatory state, and circulating progenitor cells in non-diabetic patients with essential hypertension: a comparative study between azelnidipine and amlodipine on glucose tolerance and endothelial function-a crossover trial (AGENT). Cardiovascular Diabetology 10: 79. https://doi. org/10.1186/1475-2840-10-79 [PMC] [PubMed]
■ Fukumitsu R, Minami M, Yoshida K, Nagata M, Yasui M, Higuchi S, Fujikawa R, Ikedo T, Yamagata S, Sato Y, Arai H, Yokode M, Miyamoto S (2015) Expression of Vasohibin-1 in human carotid atherosclerotic plaque. Journal of Atherosclerosis and Thrombosis 22(9): 942-948. https://doi.org/10.5551/jat.29074 [PubMed]
■ Fukumoto K, Takemoto Y, Norioka N,Takahashi K, Namikawa H, Tochino Y, Shintani F, Yoshiyama M, Shuto T (2021) Predictors of the effects of smoking cessation on the endothelial function of conduit and digital vessels. Hypertension Research 44: 63-70. https:// doi.org/10.1038/s41440-020-0516-z
■ Fukutomi M, Hoshide S, Mizuno H, Kario K (2014) Differential effects of aliskiren/amlodipine combination and high-dose amlodipine monotherapy on endothelial function in elderly hypertensive patients. American Journal of Hypertension 27(1): 14-20. https://doi. org/10.1093/ajh/hpt158 [PubMed]
■ Furchgott RF, Zawadzki JV (1980) The obligatory role of endotheli-al cells in the relaxation of arterial smooth muscle by acetylcholine. Nature 288: 373-376. https://doi.org/10.1038/288373a0 [PubMed]
■ Gamil S, Erdmann J, Schwedhelm E, Bakheit KH, Abdalrahman IBB, Mohamed AO (2020) Increased serum levels of asymmetric dimethylarginine and symmetric dimethylarginine and decreased levels of arginine in sudanese patients with essential hypertension. Kidney and Blood Pressure Research 45(5): 727-736. https://doi. org/10.1159/000508695 [PubMed]
■ Gershtein ES, Kushlinsky DN, Tereshkina IV, Ermilova VD, Ovchinnikova LK, Galdava DE, Kuznetsova OV (2015) Vascular endothelial growth factor and tumors of the female reproductive system. Part 1. Breast cancer. Oncogynecology [Onkoginekologiya] (1): 34-41. [in Russian]
■ Gontschar IA, Prudyvus IS, Stepanova YuI (2013) Vascular endothe-lial growth factor expression in patients with acute ischemic stroke. S.S. Korsakov Journal of Neurology and Psychiatry [Zhurnal Nev-rologii i Psihiatrii im. S.S. Korsakova] 3(2): 25-29. [in Russian]
■ Grabczewska Z, Nartowicz E, Kubica J, Rose D (2006) Endothe-lial function parameters in patients with unstable angina and infection with Helicobacter pylori and Chlamydia pneumoniae. European Journal of Internal Medicine 17(5): 339-342. https://doi. org/10.1016/j.ejim.2006.02.005 [PubMed]
■ Gumanova NG, Metel'skaya VA, Artyushkova EB, Kochkarov VI, Pokrovskaya TG, Danilenko LM, Korneev MM, Pokrovskii MV, Pashin EN (2007) Effect of antioxidants pQ510 and resveratrol on regulatory function of the endothelium in rats with modeled arterial hypertension. Bulletin of Experimental Biology and Medicine 143(6): 678-681. https://doi.org/10.1007/s10517-007-0212-x
■ Gureev VA, Kochkarov VI, Proskuryakova EV (2010) Correction of ADMA-like preeclampsia with resveratrol in the experiment. Actual Problems of Medicine 22(93): 51-55.
■ Habas K, Shang L (2018) Alterations in intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in human endothelial cells. Tissue and Cell 54: 139-143. https://doi. org/10.1016/j.tice.2018.09.002 [PubMed]
■ Habib JB, Bossaller C, Wells S, Williams C, Morrisett JD, Henry PD (1986) Preservation of endothelium-dependent vascular relaxation in cholesterol-fed rabbit by treatment with the calcium blocker PN 200110. Circulation Research 58: 305-309. https://doi. org/10.1161/01.RES.58.2.305 [PubMed]
■ Hadi A, Arab A, Moradi S, Pantovic A, Clark CCT, Ghaedi E (2019) The effect of l-arginine supplementation on lipid profile: a systematic review and meta-analysis of randomised controlled trials. The British Journal of Nutrition 122(9): 1021-1032. https://doi.org/10.1017/ S0007114519001855 [PubMed]
■ Halcox JP, Nour KR, Zalos G, Mincemoyer RA, Waclawiw M, Rivera CE, Willie G, Ellahham S, Quyyumi AA (2002) The effect of sildenafil on human vascular function, platelet activation, and myo-cardial ischemia. Journal of the American College of Cardiology 40: 1232-1240. https://doi.org/10.1016/S0735-1097(02)02139-3 [PubMed]
■ Hamming I, Bulthuis MLC, Lely AT (2014) Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis. The Journal of Pathology 203(2): 631-637. https://doi.org/10.1002/path.1570 [PMC] [PubMed]
■ Harker LA, Ross R, Slichter SJ, Scott CR (1976) Homocystine-in-duced arteriosclerosis. The role of endothelial cell injury and platelet response in its genesis. The Journal of Clinical Investigation 58: 731-741. https://doi.org/10.1172/JCI108520 [PMC] [PubMed]
■ He Y, Si D, Yang C, Ni L, Li B, Ding M, Yang P (2014) The effects of amlodipine and S(-)-amlodipine on vascular endothelial function in patients with hypertension. American Journal of Hypertension 27: 27-31. https://doi.org/10.1093/ajh/hpt138 [PubMed]
■ Heeschen C, Aicher A, Lehmann R, Fichtlscherer S, Vasa M, Urbich C, Mildner-Rihm C, Martin H, Zeiher AM, Dimmeler S (2003)
Erythropoietin is a potent physiologic stimulus for endothelial progenitor cell mobilization. Blood 102(4): 1340-1346. https://doi. org/10.1182/blood-2003-01-0223 [PubMed]
■ Heil SG, De Vriese AS, Kluijtmans LA, Mortier S, Den Heijer M, Blom HJ (2004) The role of hyperhomocysteinemia in nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF)-me-diated vasodilatation. Cellular and Molecular Biology 50: 911-916. [PubMed]
■ Hidalgo A, Peired AJ, Wild MK, Vestweber D, Frenette PS (2007) Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44. Immunity 26(4): 477489. https://doi.org/10.1016/j.immuni.2007.03.011 [PMC] [PubMed]
■ Hill JM, Zalos G, Halcox JP, Schenke WH, Waclawiw MA, Quyyu-mi AA, Finkel T (2003) Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. The New England Journal of Medicine 348(7): 593-600. https://doi.org/10.1056/NEJMoa022287 [PubMed]
■ Hirata M, Serizawa K, Aizawa K, Yogo K, Tashiro Y, Takeda S, Moriguchi Y, Endo K, Fukagawa M (2013) 22-Oxacalcitriol prevents progression of endothelial dysfunction through antioxidative effects in rats with type 2 diabetes and early-stage nephropathy. Nephrology, Dialysis, Transplantation 28: 1166-1174. https://doi. org/10.1093/ndt/gfs536 [PubMed]
■ Hsueh WA, Lyon CJ, Quiñones MJ (2004) Insulin resistance and the endothelium. The American Journal ofMedicine 117: 109-117. https:// doi.org/10.1016/j.amjmed.2004.02.042 [PubMed] [Google Scholar]
■ Huang A, Pinto JT, Froogh G, Kandhi S, Qin J, Wolin MS, Hintze TH, Sun D (2015) Role of homocysteinylation of ACE in endothelial dysfunction of arteries. American Journal of Physiology. Heart and Circulatory Physiology 308: H92-H100. https://doi.org/10.1152/ ajpheart.00577.2014 [PMC] [PubMed]
■ Hubbard AK, Rothlein R (2000) Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radical Biology and Medicine 28(9): 1379-1386. https://doi.org/10.1016/S0891-5849(00)00223-9 [PubMed]
■ Igarashi J, Bernier SG, Michel T (2001) Sphingosine 1-phosphate and activation of endothelial nitric-oxide synthase. differential regulation of Akt and MAP kinase pathways by EDG and brady-kinin receptors in vascular endothelial cells. The Journal of Biological Chemistry 276: 12420-12426. https://doi.org/10.1074/jbc. M008375200 [PubMed]
■ Imanishi T, Tsujioka H, Ikejima H, Kuroi A, Takarada S, Kitabata H, Tanimoto T, Muragaki Y, Mochizuki S, Goto M, Yoshida K, Akasaka T (2008) Renin inhibitor aliskiren improves impaired nitric oxide bioavailability and protects against atherosclerotic changes. Hypertension 52(3): 563-572. https://doi.org/10.1161/HYPERTENSI0N-AHA.108.111120 [PubMed]
■ Jankovic A, Korac A, Buzadzic B, Stancic A, Otasevic V, Ferdi-nandy P, Daiber A, Korac B (2017) Targeting the NO/superoxide ratio in adipose tissue: relevance to obesity and diabetes management. British Journal of Pharmacology 174: 1570-1590. https://doi. org/10.1111/bph.13498 [PMC] [PubMed]
■ Jarajapu YP, Bhatwadekar AD, Caballero S, Hazra S, Shenoy V, Medina R, Kent D, Stitt AW, Thut C, Finney EM, Raizada MK, Grant MB (2013) Activation of the ACE2/angiotensin-(1-7)/Mas receptor axis enhances the reparative function of dysfunctional diabetic endothelial progenitors. Diabetes 62: 1258-1269. https://doi. org/10.2337/db12-0808 [PMC] [PubMed]
■ Jochmann N, Schröter F, Knebel F, Hättasch R, Gericke C, Stangl K, Baumann G, Stangl V (2014) Effect of ivabradine-induced heart rate reduction on flow-mediated dilation measured with high-sensitivity ultrasound in patients with stable coronary heart disease. Cardiovascular Ultrasound 12: 5. https://doi.org/10.1186/1476-7120-12-5 [PMC] [PubMed]
■ Joffre J, Hellman J, Ince C, Ait-Oufella H (2020) Endothelial responses in sepsis. American Journal of Respiratory and Critical Care Medicine 202(3): 361-370. https://doi.org/10.1164/rc-cm.201910-1911TR [PubMed]
■ Johnstone MT, Creager SJ, Scales KM, Cusco JA, Lee BK, Crea-ger MA (1993) Impaired endothelium-dependent vasodilation in patients with insulin-dependent diabetes mellitus. Circulation 88: 2510-2516. https://doi.org/10.1161/01.CIR.88.6.2510 [PubMed]
■ Kang DH, Han L, Ouyang X, Kahn AM, Kanellis J, Li P, Feng L, Nakagawa T, Watanabe S, Hosoyamada M, Endou H, Lipkowitz M, Abramson R, Mu W, Johnson RJ (2005) Uric acid causes vascular smooth muscle cell proliferation by entering cells via a functional urate transporter. American Journal of Nephrology 25(5): 425-433. https://doi.org/10.1159/000087713 [PubMed]
■ Karbach S, Wenzel P, Waisman A, Munzel T, Daiber A (2014) eNOS uncoupling in cardiovascular diseases- the role of oxidative stress and inflammation. Current Pharmaceutical Design 20: 3579-3594. https://doi.org/10.2174/13816128113196660748 [PubMed]
■ Kasprzak JD, Klosinska M, Drozdz J (2006) Clinical aspects of assessment of endothelial function. Pharmacological Reports 58: 33-40. [PubMed]
■ Kass DA, Champion HC, Beavo JA (2007) Phosphodiesterase type 5: expanding roles in cardiovascular regulation. Circulation Research 101: 1084-1095. https://doi.org/10.1161/CIRCRESA-HA.107.162511 [PubMed]
■ Kassi E, Adamopoulos C, Basdra EK, Papavassiliou AG (2013) Role of vitamin D in atherosclerosis. Circulation 128: 2517-2531. https:// doi.org/10.1161/CIRCULATIONAHA.113.002654 [PubMed]
■ Kavoussi PK, Smith RP, Oliver JL, Costabile RA, Steers WD, Brown-Steinke K, de Ronde K, Lysiak JJ, Palmer LA (2019) S-nitrosylation of endothelial nitric oxide synthase impacts erectile function. International Journal of Impotence Research 31(1): 31-38. https://doi.org/10.1038/s41443-018-0056-0 [PubMed]
■ Kelly AS, Gonzalez-Campoy JM, Rudser KD, Katz H, Metzig AM, Thalin M, Bank AJ (2012) Carvedilol-lisinopril combination therapy and endothelial function in obese individuals with hypertension. The Journal of Clinical Hypertension 14: 85-91. https://doi.org/10.1111/ j.1751-7176.2011.00569.x [PMC] [PubMed]
■ Khadieva TA, Pokrovskaya TG, Belousova Y (2019) Pharmacological correction of endothelial dysfunction using ademethionin and taurine. Research Results in Pharmacology 5(2): 13-21. https://doi. org/10.3897/rrpharmacology. 5.32730
■ Khan A, Dawoud H, Malinski T (2018) Nanomedical studies of the restoration of nitric oxide/peroxynitrite balance in dysfunctional endothelium by 1,25-dihydroxy vitamin D3 - clinical implications for cardiovascular diseases. International Journal of Nanomedicine 13: 455-466. https://doi.org/10.2147/IJN.S152822 [PMC] [PubMed]
■ Khomich OA, Kochetkov SN, Bartosch B (2018) Redox biology of respiratory viral infections. Viruses 10(8): 392. https://doi. org/10.3390/v10080392 [PMC] [PubMed]
■ Kim JA, Montagnani M, Chandrasekran S, Quon MJ (2012) Role of lipotoxicity in endothelial dysfunction. Heart Failure Clinics
8(4): 589-607. https://doi.org/10.1016/j.hfc.2012.06.012 [PMC] [PubMed]
■ Kimmel SE, Schelleman H, Berlin JA, Oslin DW, Weinstein RB, Kinman JL, Sauer WH, Lewis JD (2011) The effect of selective serotonin re-uptake inhibitors on the risk of myocardial infarction in a cohort of patients with depression. British Journal of Clinical Pharmacology 72(3): 514-517. https://doi.org/10.1111/j.1365-2125.2011.04008.x [PMC] [PubMed]
■ Kochkarov VI, Pokrovsky MV, Pokrovskaya TG, Gudyrevi OS (2008) Cardioprotective effects of resveratrol during experimental blockade of the inducible nitric oxide synthase Allergology and Immunology 9(1): 157. [in Russian]
■ Koike S, Ogasawara Y (2016) Sulfur atom in its bound state is a unique element involved in physiological functions in mammals. Molecules 21(12): 1753. https://doi.org/10.3390/mole-cules21121753 [PMC] [PubMed]
■ Konovalova EA, Chernomortseva ES (2019) The study of the en-dothelial protective properties of the L-norvaline combination with mexidol in the simulation of L-NAME-induced NO deficiency. Research Results in Pharmacology 5(4): 41-46. https://doi.org/10.3897/ rrpharmacology. 5.38405
■ Konukoglu D, Uzun H (2017) Endothelial dysfunction and hypertension. Advances in Experimental Medicine and Biology 956: 511540. https://doi.org/10.1007/5584_2016_90 [PubMed]
■ Kosacka M, Brzecka A (2021) Endothelin-1 and LOX-1 as markers of endothelial dysfunction in obstructive sleep apnea patients. International Journal of Environmental Research and Public Health 18(3): 1319. https://doi.org/10.3390/ijerph18031319 [PMC] [PubMed]
■ Kosmidou I, Moore JP, Weber M, Searles CD (2007) Statin treatment and 3' polyadenylation of eNOS mRNA. Arteriosclerosis, Thrombosis, and Vascular Biology 27: 2642-2649. https://doi.org/10.1161/ ATVBAHA.107.154492 [PubMed]
■ Kureishi Y, Luo Z, Shiojima I, Bialik A, Fulton D, Lefer DJ, Sessa WC, Walsh K (2000) The HMG-CoA reductase inhibitor simvastatin activates the protein kinase Akt and promotes angiogenesis in nor-mocholesterolemic animals. Nature Medicine 6: 1004-1010. https:// doi.org/10.1038/79510 [PMC] [PubMed]
■ Kurtz TW, DiCarlo SE, Pravenec M, Morris RC (2018) Functional foods for augmenting nitric oxide activity and reducing the risk for salt-induced hypertension and cardiovascular disease in Japan. Journal of Cardiology 72(1): 42-49. https://doi.org/10.1016/j. jjcc.2018.02.003 [PMC] [PubMed]
■ Lai WT, Lee KT, Chu CS, Voon WC, Yen HW, Tsai LY, Sheu SH (2005) Influence of withdrawal of statin treatment on proinflam-matory response and fibrinolytic activity in humans: an effect independent on cholesterol elevation. International Journal of Cardiology 98: 459-464. https://doi.org/10.1016/j.ijcard.2003.11.023 [PubMed]
■ Lange C, Storkebaum E, de Almodovar CR, Dewerchin M, Carme-liet P (2016) Vascular endothelial growth factor: a neurovascular target in neurological diseases. Nature Reviews. Neurology 12(8): 439-454. https://doi.org/10.1038/nrneurol.2016.88 [PubMed]
■ Lavi S, Prasad A, Yang EH, Mathew V, Simari RD, Rihal CS, Lerman LO, Lerman A (2007) Smoking is associated with epicardi-al coronary endothelial dysfunction and elevated white blood cell count in patients with chest pain and early coronary artery disease. Circulation 115: 2621-2627. https://doi.org/10.1161/CIRCULA-TIONAHA.106.641654 [PubMed]
■ Lee W, Ku SK, Kim SW, Bae JS (2014) Endocan elicits severe vascular inflammatory responses in vitro and in vivo. Journal of Cellular Physiology 229: 620-630. https://doi.org/10.1002/jcp.24485 [PubMed]
■ Leite AR, Borges-Canha M, Cardoso R, Neves JS, Castro-Ferreira R, Leite-Moreira A (2020) Novel biomarkers for evaluation of endothelial dysfunction. Angiology 71(5): 397-410. https://doi. org/10.1177/0003319720903586 [PubMed]
■ Lewis GD, Lachmann J, Camuso J, Lepore JJ, Shin J, Martinovic ME, Systrom DM, Bloch KD, Semigran MJ (2007) Sildenafil improves exercise hemodynamics and oxygen uptake in patients with systolic heart failure. Circulation 115: 59-66. https://doi. org/10.1161/CIRCULATI0NAHA.106.626226 [PubMed]
■ Li H, Xia N, Hasselwander S, Daiber A (2019) Resveratrol and vascular function. International Journal of Molecular Sciences 20(9): 2155. https://doi.org/10.3390/ijms20092155 [PubMed] [PMC]
■ Li J, White J, Guo L, Zhao X, Wang J, Smart EJ, Li XA (2009) Salt inactivates endothelial nitric oxide synthase in endothelial cells. The Journal of Nutrition 139 (3): 447-451. https://doi.org/10.3945/ jn.108.097451 [PMC] [PubMed]
■ Li L, Roumeliotis N, Sawamura T, Renier G (2004) C-reactive protein enhances LOX-1 expression in human aortic endothelial cells: relevance of LOX-1 to C-reactive protein-induced endothelial dysfunction. Circulation Research 95: 877-883. https://doi. org/10.1161/01.RES.0000147309.54227.42 [PubMed]
■ Li W, Li R, Zhao S, Jiang C, Liu Z, Tang X (2018) Lithium posttreat-ment alleviates blood-brain barrier injury after intracerebral hemorrhage in rats. Neuroscience 383: 129-137. https://doi.org/10.1016/j. neuroscience.2018.05.001 [PubMed]
■ Little PJ, Askew CD, Xu S, Kamato D (2021) Endothelial dysfunction and cardiovascular disease: history and analysis of the clinical utility of the relationship. Biomedicines 9(6): 699. https://doi. org/10.3390/biomedicines9060699 [PMC]
■ Liu S, Yuan Y, Zhou Y, Zhao M, Chen Y, Cheng J, Lu Y, Liu J (2017) Phloretin attenuates hyperuricemia-induced endothelial dysfunction through co-inhibiting inflammation and GLUT9-mediated uric acid uptake. Journal of Cellular and Molecular Medicine 21(10): 25532562. https://doi.org/10.1111/jcmm.13176 [PMC] [PubMed]
■ Lopez-Vilchez I, Diaz-Ricart M, Navarro V, Torramade S, Zamora-no-Leon J, Lopez-Farre A, Galan AM, Gasto C, Escolar G (2016) Endothelial damage in major depression patients is modulated by SSRI treatment, as demonstrated by circulating biomarkers and an in vitro cell model. Translational Psychiatry 6(9): e886. https://doi. org/10.1038/tp.2016.156 [PMC] [PubMed]
■ Lucke S, Levkau B (2010) Endothelial functions of sphin-gosine-1-phosphate. Cellular Physiology and Biochemistry 26: 87-96. https://doi.org/10.1159/000315109 [PubMed]
■ Lyoo IK, Dager SR, Kim JE, Yoon SJ, Friedman SD, Dunner DL, Renshaw PF (2010) Lithium-induced gray matter volume increase as a neural correlate of treatment response in bipolar disorder: a longitudinal brain imaging study. Neuropsychopharmacology 35(8): 1743-1750. https://doi.org/10.1038/npp.2010.41 [PMC] [PubMed]
■ Mahat RK, Singh N, Gupta A, Rathore V (2018) Oxidative DNA damage and carotid intima media thickness as predictors of cardiovascular disease in prediabetic subjects. Journal of Cardiovascular Development and Disease 5(1): 15. https://doi.org/10.3390/ jcdd5010015 [PMC] [PubMed]
■ Mallela J, Perkins R, Yang J, Pedigo S, Rimoldi JM, Shariat-Ma-dar Z (2008) The functional importance of the N-terminal region of human prolylcarboxypeptidase. Biochemical and Biophysical Research Communications 374: 635-640. https://doi.org/10.1016/j. bbrc.2008.07.069 [PMC] [PubMed]
■ Markov HM (2005) Molecular mechanisms of vascular endothelial dysfunction. Cardiology [Kardiologiya] 12: 62-67. [in Russian]
■ Martelli A, Testai L, Citi V, Marino A, Bellagambi FG, Ghimenti S, Breschi MC, Calderone V (2014) Pharmacological characterization of the vascular effects of aryl isothiocyanates: is hydrogen sulfide the real player? Vascular Pharmacolog 60(1): 32-41. https://doi. org/10.1016/j.vph.2013.11.003 [PubMed]
■ Martínez-González J, Raposo B, Rodríguez C, Badimon L (2001) 3-hydroxy-3-methylglutaryl coenzyme a reductase inhibition prevents endothelial NO synthase downregulation by atherogenic levels of native LDLs: balance between transcriptional and posttranscrip-tional regulation. Arteriosclerosis, Thrombosis, and Vascular Biology 21: 804-809. https://doi.org/10.1016Zj.bbrc.2008.07.069 [PubMed]
■ Martins-Filho RK, Zotin MC, Rodrigues G, Pontes-Neto O (2020) Bio-markers related to endothelial dysfunction and vascular cognitive impairment: a systematic review. Dementia and Geriatric Cognitive Disorders 49(4): 365-374. https://doi.org/10.1159/000510053 [PubMed]
■ Maruhashi T, Hisatome I, Kihara Y, Higashi Y (2018) Hyperuricemia and endothelial function: from molecular background to clinical perspectives. Atherosclerosis 278: 226-231. https://doi.org/10.1016/j. atherosclerosis.2018.10.007 [PubMed]
■ Marzolla V, Armani A, Mammi C, Moss ME, Pagliarini V, Ponte-corvo L, Antelmi A, Fabbri A, Rosano G, Jaffe IZ, Caprio M (2017) Essential role of ICAM-1 in aldosterone-induced atherosclerosis. International Journal of Cardiology 232: 233-242. https://doi. org/10.1016/j.ijcard.2017.01.013 [PMC] [PubMed]
■ Matsubara M, Hasegawa K (2005) Benidipine, a dihydropyri-dine-calcium channel blocker, prevents lysophosphatidylcholine-in-duced injury and reactive oxygen species production in human aortic endothelial cells. Atherosclerosis 178: 57-66. https://doi. org/10.1016/j.atherosclerosis.2004.08.020 [PubMed]
■ Matsumoto T, D'uscio LV, Eguchi D, Akiyama M, Smith LA, Katu-sic ZS (2003) Protective effect of chronic vitamin C treatment on endothelial function of apolipoprotein E-deficient mouse carotid artery. The Journal of Pharmacology and Experimental Therapeutics 306: 103-108. https://doi.org/10.1124/jpet.103.049163 [PubMed]
■ Melnikova YS, Makarova TP (2015) Endothelial dysfunction as a central link in the pathogenesis of chronic diseases. Kazan Medical Journal [Kazanskii Meditsinskii ZHurnal] 96(4): 659-665. https:// doi.org/10.17750/KMJ2015-659 [in Russian]
■ Mineo C, Deguchi H, Griffin JH, Shaul PW (2006) Endothelial and antithrombotic actions of HDL. Circulation Research 98: 1352-1364. https://doi.org/10.1161/01.RES.0000225982.01988.93 [PubMed]
■ Miyashita H, Watanabe T, Hayashi H, Suzuki Y, Nakamura T, Ito S, Ono M, Hoshikawa Y, Okada Y, Kondo T, Sato Y (2012) Angiogen-esis inhibitor vasohibin-1 enhances stress resistance of endothelial cells via induction of SOD2 and SIRT1. PLoS ONE 7(10): e46459. https://doi.org/10.1371/journal.pone.0046459 [PMC][PubMed]
■ Mohammadianinejad SE, Majdinasab N, Sajedi SA, Abdollahi F, Moqaddam MM, Sadr F (2014) The effect of lithium in post-stroke motor recovery: a double-blind, placebo-controlled, randomized clinical trial. Clinical Neuropharmacology 37(3): 73-78. https://doi. org/10.1097/WNF.0000000000000028 [PubMed]
■ Molchanova EE (2016) The possibilities of non-pharmacological correction of endothelial dysfunction in the acute period of ischemic stroke. Amur Medical Journal [Amurskii Meditsinskiy Zhurnal] 3-4(15-16): 91-92. https://doi.org/10.22448/AMJ.2016.15-16.91-92 [in Russian]
■ Moncada S, Higgs EA (2006) Nitric oxide and the vascular endothelium. Handbook of experimental pharmacology 176(Pt 1): 213-254. https://doi.org/10.1007/3-540-32967-6_7 [PubMed]
■ Montana V, Sontheimer H (2011) Bradykinin promotes the chemo-tactic invasion of primary brain tumors. The Journal of Neuroscience 31: 4858-4867. https://doi.org/10.1523/JNEUROSCI.3825-10.2011 [PMC] [PubMed]
■ Monteil V, Kwon H., Prado P (2020) Inhibition of SARS-CoV-2 infections in engineered human tissues using clinical-grade soluble human ACE2. Cell 181(4): 905-913. https://doi.org/10.1016/j. cell.2020.04.004 [PMC] [PubMed]
■ Morris G, Puri BK, Olive L, Carvalho A, Berk M, Walder K, Gustad LT, Maes M (2020) Endothelial dysfunction in neuroprogressive disorders-causes and suggested treatments. BMC Medicine 18(1): 305. https://doi.org/10.1186/s12916-020-01749-w [PMC] [PubMed]
■ Moshal KS, Sen U, Tyagi N, Henderson B, Steed M, Ovechkin AV, Tya-gi SC (2006) Regulation of homocysteine-induced MMP-9 by ERK1/2 pathway. American Journal of Physiology. Cell Physiology 290: C883-C891. https://doi.org/10.1152/ajpcell.00359.2005 [PubMed]
■ Münzel T, Hahad O, Kuntic M, Keaney JF, Deanfield JE, Daiber A (2020) Effects of tobacco cigarettes, e-cigarettes, and waterpipe smoking on endothelial function and clinical outcomes. European Heart Journal 41(41): 4057-4070. https://doi.org/10.1093/eurheartj/ ehaa460 [PMC] [PubMed]
■ Murdaca G, Colombo BM, Cagnati P, Gulli R, Spano F, Puppo F (2012) Endothelial dysfunction in rheumatic autoimmune diseases. Atherosclerosis 224(2): 309-317. https://doi.org/10.1016Zj.athero-sclerosis.2012.05.013 [PubMed]
■ Murmu M, Karun MKP, Dash S, Singh LK, Kar A, Mishra PK (2018) Study of serum homocysteine level in cases of non-diabetic ischemic stroke. International Journal of Research in Medical Sciences 6(5): 1611-1616. https://doi.org/10.18203/2320-6012.ijrms20181433
■ Musikhina NA, Gapon LI, Utesheva AB, Petelina TI, Kolesnikova SN (2012) Cerebral blood flow and endothelial functional activity in patients with coronary heart disease and arterial hypertension during therapy with ivabradine in combination with perindopril. Therapeutic Archives [Terapevticheskii Arkhiv] 84: 30-34. [PubMed] [in Russian]
■ Mustafa AK, Gadalla MM, Sen N, Kim S, Mu W, Gazi SK, Barrow RK, Yang G, Wang R, Snyder SH (2009) H2S signals through protein S-sulfhydration. Science Signaling 2(96): ra72. https://doi. org/10.1126/scisignal.2000464 [PMC] [PubMed]
■ Natarajan V, Dudek SM, Jacobson JR, Moreno-Vinasco L, Huang LS, Abassi T, Mathew B, Zhao Y, Wang L, Bittman R, Weichselbaum R, Berdyshev E, Garcia JG (2013) Sphingosine-1-phosphate, FTY720, and sphingosine-1-phosphate receptors in the pathobiolo-gy of acute lung injury. American Journal of Respiratory Cell and Molecular Biology 49(1): 6-17. https://doi.org/10.1165/rcmb.2012-0411TR [PMC] [PubMed]
■ Nerla R, Di Franco A, Milo M, Pitocco D, Zaccardi F, Tarzia P, Sarullo FM, Villano A, Russo G, Stazi A, Ghirlanda G, Lanza GA, Crea F (2012) Differential effects of heart rate reduction by atenolol or ivabradine on peripheral endothelial function in type 2 diabetic patients. Heart 98(24): 1812-1816. https://doi.org/10.1136/heart-jnl-2012-302795 [PubMed]
■ Nishimatsu H, Suzuki E, Takeda R, Takahashi M, Oba S, Kimura K, Nagano T, Hirata Y (2008) Blockade of endogenous proinflammatory cytokines ameliorates endothelial dysfunction in obese Zucker rats. Hypertension Research 31(4): 737-743. https://doi. org/10.1291/hypres.31.737 [PubMed]
■ Nystoriak MA, Bhatnagar A (2018) Cardiovascular effects and benefits of exercise. Frontiers in Cardiovascular Medicine 5: 135. https:// doi.org/10.3389/fcvm.2018.00135 [PMC] [PubMed]
■ Okamura T, Tawa M, Geddawy A, Shimosato T, Iwasaki H, Shintaku H, Yoshida Y, Masada M, Shinozaki K, Imamura T (2014) Effects of atorvastatin, amlodipine, and their combination on vascular dysfunction in insulin-resistant rats. Journal of Pharmacological Sciences 124: 76-85. https://doi.org/10.1254/jphs.13178FP [PubMed]
■ Orea-Tejeda A, Balderas-Muñoz K, Castillo-Martínez L, Infante-Vázquez O, Martínez Memije R, Keirns-Davis C, Dorantes-García J, Narváez-David R, Vázquez-Ortíz Z (2013) Effect of ivabradine on endothelial function in diastolic and right heart failure patients. Cardiology Research and Practice 2013: 603913. https:// doi.org/10.1155/2013/603913 [PMC] [PubMed]
■ Ou J, Ou Z, McCarver DG, Hines RN, Oldham KT, Ackerman AW, Pritchard KA (2003) Trichloroethylene decreases heat shock protein 90 interactions with endothelial nitric oxide synthase: implications for endothelial cell proliferation. Toxicological Sciences 73: 90-97. https://doi.org/10.1093/toxsci/kfg062 [PubMed]
■ Oz F, Cizgici AY, Oflaz H, Elitok A, Karaayvaz EB, Mercanoglu F, Bugra Z, Omer B, Adalet K, Oncul A (2013) Impact of vitamin D insufficiency on the epicardial coronary flow velocity and endothelial function. Coronary Artery Disease 24: 392-397. https://doi. org/10.1097/MCA.0b013e328362b2c8 [PubMed]
■ Panza JA, Casino PR, Kilcoyne CM, Quyyumi AA (1993) Role of endothelium-derived nitric oxide in the abnormal endothelium-de-pendent vascular relaxation of patients with essential hypertension. Circulation 87(5): 1468-1474. https://doi.org/10.1161/01. CIR.87.5.1468 [PubMed]
■ Parsamanesh N, Asghari A, Sardari S, Tasbandi A, Jamialahmadi T, Xu S, Sahebkar A (2021) Resveratrol and endothelial function: A literature review. Pharmacological Research 170: 105725. https://doi. org/10.1016/j.phrs.2021.105725 [PubMed]
■ Pennathur S, Heinecke JW (2007) Oxidative stress and endotheli-al dysfunction in vascular disease. Current Diabetes Reports 7(4): 257-264. https://doi.org/10.1007/s11892-007-0041-3 [PubMed]
■ Pernomian L, Gomes MS, Restini CB, de Oliveira AM (2014) MAS-mediated antioxidant effects restore the functionality of angio-tensin converting enzyme 2-angiotensin-(1-7)-MAS axis in diabetic rat carotid. BioMed Research International 2014: 640329. https:// doi.org/10.1155/2014/640329 [PMC] [PubMed]
■ Perros F, Ranchoux B, Izikki M, Bentebbal S, Happé C, Antigny F, Jourdon P, Dorfmüller P, Lecerf F, Fadel E, Simonneau G, Humbert M, Bogaard HJ, Eddahibi S (2015) Nebivolol for improving endothelial dysfunction, pulmonary vascular remodeling, and right heart function in pulmonary hypertension. Journal of the American College of Cardiology 65: 668-680. https://doi.org/10.1016/j. jacc.2014.11.050 [PubMed] [Google Scholar]
■ Pizzi C, Mancini S, Angeloni L, Fontana F, Manzoli L, Costa GM (2009) Effects of selective serotonin reuptake inhibitor therapy on endothelial function and inflammatory markers in patients with coronary heart disease. Clinical Pharmacology and Therapeutics 86(5): 527-532. https://doi.org/10.1038/clpt.2009.121 [PubMed]
■ Polverino F, Celli BR, Owen CA (2018) COPD as an endothelial disorder: endothelial injury linking lesions in the lungs and other organs? Pulmonary Circulation 8(1): 2045894018758528. https://doi. org/10.1177/2045894018758528 [PMC] [PubMed]
■ Pothineni NVK, Subramany S, Kuriakose K, Shirazi LF, Romeo F, Shah PK, Mehta JL (2017) Infections, atherosclerosis, and coronary heart disease. European Heart Journal 38(43): 3195-3201. https:// doi.org/10.1093/eurheartj/ehx362 [PubMed]
■ Prasad K, Dhar I, Caspar-Bell G (2015) Role of advanced glyca-tion end products and its receptors in the pathogenesis of cigarette smoke-induced cardiovascular disease. The International Journal of Angiology 24(2): 75-80. https://doi.org/10.1055/s-0034-1396413 [PMC] [PubMed]
■ Price KL, Sautin YY, Long DA (2006) Human vascular smooth muscle cells express a urate transporter. Journal of the American Society of Nephrology 17(7): 1791-1795. https://doi.org/10.1681/ ASN.2006030264 [PubMed]
■ Proietti M, Aversa A, Letizia C, Rossi C, Menghi G, Bruzziches R, Merla A, Spera G, Salsano F (2007) Erectile dysfunction in systemic sclerosis: effects of longterm inhibition of phosphodiesterase type-5 on erectile function and plasma endothelin-1 levels. The Journal of Rheumatology 34(8): 1712-1717. [PubMed]
■ Rahimi N (2017) Defenders and challengers of endothelial barrier function. Frontiers in Immunology 8: 1847. https://doi.org/10.3389/ fimmu.2017.01847 [PMC] [PubMed]
■ Rikitake Y, Liao JK (2005) Rho GTPases, statins, and nitric oxide. Circulation Research 97(12): 1232-1235. https://doi.org/10.1161/01. RES.0000196564.18314.23 [PMC] [PubMed]
■ Ritchie RH, Irvine JC, Rosenkranz AC, Patel R, Wendt IR, Horowitz JD, Kemp-Harper BK (2009) Exploiting cGMP-based therapies for the prevention of left ventricular hypertrophy: NO* and beyond. Pharmacology and Therapeutics 124(3): 279-300. https://doi. org/10.1016/j.pharmthera.2009.08.001 [PubMed]
■ Roberts CK, Vaziri ND, Wang XQ, Barnard RJ (2000) Enhanced NO inactivation and hypertension induced by a highfat, refinedcarbohy-drate diet. Hypertension 36(3): 423-429. https://doi.org/10.1161/01. HYP.36.3.423 [PubMed]
■ Roorda M, Miljkovic JL, van Goor H, Henning RH, Bouma HR (2021) Spatiotemporal regulation of hydrogen sulfide signaling in the kidney. Redox Biology 43: 101961. https://doi.org/10.1016Zj.re-dox.2021.101961 [PubMed] [PMC]
■ Rosato E, Letizia C, Proietti M, Aversa A, Menghi G, Rossi C, Torella E, Cotesta D, Petramala L, Bruzziches R, Spera G, Pisarri S, Salsano F (2009) Plasma adrenomedullin and endothelin-1 levels are reduced and Raynaud's phenomenon improved by daily tadala-fil administration in male patients with systemic sclerosis. Journal of Biological Regulators and Homeostatic Agents 23(1): 23-29. [PubMed]
■ Sabri M, Ghaffari G, Hashemipour M, Mostofizadeh N, Koushki AM (2016) Effect of long-term Vitamin C intake on vascular en-dothelial function in diabetic children and adolescents: A pilot study. Journal of Research in Medical Sciences 21: 119. https://doi. org/10.4103/1735-1995.193510 [PMC] [PubMed]
■ Safronenko AV, Lepyavka SV, Demidov IA, Nazheva MI, Maklya-kov YuS (2021) Optimization of premedication of patients with arterial hypertension and severe ventricular rhythm disturbances with Amiodarone-associated thyrotoxicosis. Research Results in Pharmacology 7(4): 55-62. https://doi.org/10.3897/rrpharmacology.7.78137
■ Salloum F, Yin C, Xi L, Kukreja RC (2003) Sildenafil induces delayed preconditioning through inducible nitric oxide synthase-depen-dent pathway in mouse heart. Circulation Research 92(6): 595-597. https://doi.org/10.1161/01.RES.0000066853.09821.98 [PubMed]
■ Salsano F, Letizia C, Proietti M, Rossi C, Proietti AR, Rosato E, Pisarri S (2005) Significant changes of peripheral perfusion and plasma adrenomedullin levels in N-acetylcysteine long term treatment of patients with sclerodermic Raynauds phenomenon. International Journal of Immunopathology and Pharmacology 18(4): 761-770. https://doi.org/10.1177/039463200501800420 [PubMed]
■ Salvio G, Ciarloni A, Cutini M, Balercia G (2021) Hyperhomocyste-inemia: Focus on endothelial damage as a cause of erectile dysfunction. International Journal of Molecular Sciences 22(1): 418. https:// doi.org/10.3390/ijms22010418 [PMC] [PubMed]
■ Sampaio WO, Henrique de Castro C, Santos RA, Schiffrin EL, Touyz RM (2007a) Angiotensin-(1-7) counterregulates angiotensin II signaling in human endothelial cells. Hypertension 50(6): 1093-1098. https:// doi.org/10.1161/HYPERTENSIONAHA.106.084848 [PubMed]
■ Sampaio WO, Souza dos Santos RA, Faria-Silva R, da Mata Machado LT, Schiffrin EL, Touyz RM (2007b) Angiotensin-(1-7) through receptor Mas mediates endothelial nitric oxide synthase activation via Akt-dependent pathways. Hypertension 49(1): 185-192. https:// doi.org/10.1161/01.HYP.0000251865.35728.2f [PubMed]
■ Sánchez A, Martínez P, Muñoz M, Benedito S, García-Sacristán A, Hernández M, Prieto D (2014) Endothelin-1 contributes to endothe-lial dysfunction and enhanced vasoconstriction through augmented superoxide production in penile arteries from insulin-resistant obese rats: role of ET(A) and ET(B) receptors. British Journal of Pharmacology 171(24): 5682-5695. https://doi.org/10.1111/bph.12870 [PMC] [PubMed]
■ Sánchez-Quesada JL, Otal-Entraigas C, Franco M, Jorba O, González-Sastre F, Blanco-Vaca F, Ordóñez-Llanos J (1999) Effect of simvastatin treatment on the electronegative low-density lipopro-tein present in patients with heterozygous familial hypercholesterol-emia. The American Journal of Cardiology 84(6): 655-659. https:// doi.org/10.1016/S0002-9149(99)00411-7 [PubMed]
■ Santangelo A, Testai M, Barbagallo P, Crisafulli C, Grasso S, Manuele S, Muscarà G, Rizzotto M, Tomarchio M, Maugeri D (2009) Use of specific serotonin reuptake inhibitors (SSRIs) (sertraline or citalopram) in the treatment of depression reduces the cardiovascular risk in the elderly: evidence from a Sicilian population >80 years recovered in the assisted sanitary residences (RSA). Archives of Gerontology and Geriatrics 48(3): 350-352. https://doi.org/10.1016/j. archger.2008.02.014 [PubMed]
■ Santi D, Spaggiari G, Greco C, Lazzaretti C, Paradiso E, Casarini L, Poti F, Brigante G, Simoni M (2021) The "Hitchhiker's Guide to the Galaxy" of endothelial dysfunction markers in human fertility. International Journal of Molecular Sciences 22(5): 2584. https://doi. org/10.3390/ijms22052584 [PMC] [PubMed]
■ Sapira V, Cojocaru IM, Lilios G, Grigorian M, Cojocaru M (2010) Study of endothelin-1 in acute ischemic stroke. Romanian Journal of Internal Medicine 48(4): 329-332. [PubMed]
■ Schäfer A, Fraccarollo D, Pförtsch S, Flierl U, Vogt C, Pfrang J, Kobsar A, Renné T, Eigenthaler M, Ertl G, Bauersachs J (2008) Improvement of vascular function by acute and chronic treatment with the PDE-5 inhibitor sildenafil in experimental diabetes mellitus. British Journal of Pharmacology 153(5): 886-893. https://doi. org/10.1038/sj.bjp.0707459 [PubMed]
■ Schäfer A, Fraccarollo D, Widder J, Eigenthaler M, Ertl G, Bauersachs J (2009) Inhibition of platelet activation in rats with severe congestive heart failure by a novel endothelial nitric oxide synthase transcription enhancer. European Journal of Heart Failure 11(4): 336-341. https://doi.org/10.1093/eurjhf/hfp005 [PubMed]
■ Scheuer H, Gwinner W, Hohbach J, Gröne EF, Brandes RP, Malle E, Olbricht CJ, Walli AK, Gröne HJ (2000) Oxidant stress in hyper-lipidemia-induced renal damage. American Journal of Physiology. Renal Physiology 278(1): F63-F74. https://doi.org/10.1152/ajpre-nal.2000.278.1.F63 [PubMed]
■ Schlaich MP, Parnell MM, Ahlers BA, Finch S, Marshall T, Zhang WZ, Kaye DM (2004) Impaired L-arginine transport and endo-thelial function in hypertensive and genetically predisposed nor-motensive subjects. Circulation 110(24): 3680-3686. https://doi. org/10.1161/01.CIR.0000149748.79945.52 [PubMed]
■ Scioli MG, Bielli A, Agostinelli S, Tarquini C, Arcuri G, Ferlosio A, Costanza G, Doldo E, Orlandi A (2014) Antioxidant treatment prevents serum deprivation- and TNF-a-induced endothelial dysfunction through the inhibition of NADPH oxidase 4 and the restoration of ß-oxidation. Journal of Vascular Research 51(5): 327-337. https:// doi.org/10.1159/000365926 [PubMed]
■ Shih CC, Shih YL, Chen JY (2021) The association between homo-cysteine levels and cardiovascular disease risk among middle-aged and elderly adults in Taiwan. BMC Cardiovascular Disorders 21(1): 191. https://doi.org/10.1186/s12872-021-02000-x [PMC] [PubMed]
■ Si H, Liu D (2008) Genistein, a soy phytoestrogen, upregulates the expression of human endothelial nitric oxide synthase and lowers blood pressure in spontaneously hypertensive rats. The Journal of Nutrition 138(2): 297-304. https://doi.org/10.1093/jn/138.2.297 [PubMed]
■ Siow YL, Au-Yeung KK, Woo CW, O K (2006) Homocysteine stimulates phosphorylation of NADPH oxidase p47phox and p67phox subunits in monocytes via protein kinase ßbeta activation. The Biochemical Journal 398(1): 73-82. https://doi.org/10.1042/ BJ20051810 [PMC] [PubMed]
■ Skyrme-Jones RA, O'Brien RC, Berry KL, Meredith IT (2000) Vitamin E supplementation improves endothelial function in type I diabetes mellitus: a randomized, placebo-controlled study. Journal of the American College of Cardiology 36(1): 94-102. https://doi. org/10.1016/S0735-1097(00)00720-8 [PubMed]
■ Smith AR, Hagen TM (2003) Vascular endothelial dysfunction in aging: loss of Akt-dependent endothelial nitric oxide synthase phosphorylation and partial restoration by (R)-alpha-lipoic acid. Biochemical Society Transactions 31(6): 1447-1449. https://doi. org/10.1042/bst0311447 [PubMed]
■ Sokolov EI, Grishina TI, Shtin SR (2013) Effect of von Willebrand factor and endothelin-1 on formation of thrombotic status in patients with ischemic heart disease. Kardiologiya 53(3): 25-30. [PubMed]
■ Soldatos G, Cooper ME, Jandeleit-Dahm KA (2005) Advanced-gly-cation end products in insulin-resistant states. Current Hypertension Reports 7(2): 96-102. https://doi.org/10.1007/s11906-005-0081-5 [PubMed]
■ Soldatov VO, Malorodova TN, Balamutova TI, Ksenofontov AO, Dovgan AP, Urozhevskaya ZS (2018) Endothelial dysfunction: comparative evaluation of ultrasound dopplerography, laser doppler-flowmetry and direct monitoring of arterial pressure for conducting pharmacological tests in rats. Research Result in Pharmacology 4(1): 73-80. https://doi.org/10.3897/rrpharmacology.4.25529
■ Sorrentino FS, Matteini S, Bonifazzi C, Sebastiani A, Parmeggiani F (2018) Diabetic retinopathy and endothelin system: microangiopathy versus endothelial dysfunction. Eye 32(7): 1157-1163. https:// doi.org/10.1038/s41433-018-0032-4 [PubMed]
■ Spieker LE, Flammer AJ, Luscher TF (2006) The vascular endotheli-um in hypertension. Handbook of Experimental Pharmacology 176(Pt 2): 249-283. https://doi.org/10.1007/3-540-36028-X_8 [PubMed]
■ Squadrito F, Altavilla D, Morabito N, Crisafulli A, D'Anna R, Cor-rado F, Ruggeri P, Campo GM, Calapai G, Caputi AP, Squadrito G (2002) The effect of the phytoestrogen genistein on plasma nitric oxide concentrations, endothelin-1 levels and endothelium dependent vasodilation in postmenopausal women. Atherosclerosis 163(2): 339-347. https://doi.org/10.1016/S0021-9150(02)00013-8 [PubMed]
■ Stasch JP, Schmidt PM, Nedvetsky PI, Nedvetskaya TY, HS AK, Meurer S, Deile M, Taye A, Knorr A, Lapp H, Müller H, Turgay Y, Rothkegel C, Tersteegen A, Kemp-Harper B, Müller-Esterl W, Schmidt HH (2006) Targeting the heme-oxidized nitric oxide receptor for selective vasodilatation of diseased blood vessels. The Journal of Clinical Investigation 116(9): 2552-2561. https://doi. org/10.1172/JCI28371 [PMC] [PubMed]
■ Stegbauer J, Potthoff SA, Quack I, Mergia E, Clasen T, Friedrich S, Vonend O, Woznowski M, Königshausen E, Sellin L, Rump LC (2011) Chronic treatment with angiotensin-(1-7) improves renal endothelial dysfunction in apolipoproteinE-deficient mice. British Journal of Pharmacology 163(5): 974-983. https://doi.org/10.1111/ j.1476-5381.2011.01295.x [PMC] [PubMed]
■ Stepanova TV, Ivanov AN, Tereshkina NE, Popyhova EB, Laguti-na DD (2019) Markers of endothelial dysfunction: pathogenetic role and diagnostic significance. Clinical Laboratory Diagnostics [Klinicheskaia Laboratornaia Diagnostika] 64(1): 34-41. https://doi. org/10.18821/0869-2084-2019-64-1-34-41 [in Russian]
■ Storch AS, Joäo Dario de Mattos, Alves R, Iuri dos Santos Galdino, Rocha HNM (2017) Methods of endothelial function assessment: description and applications. International Journal of Cardiovascular Sciences 30(3): 262-273. https://doi.org/10.5935/2359-4802.20170034
■ Straface E, Lista P, Gambardella L, Franconi F, Malorni W (2010) Gender-specific features of plasmatic and circulating cell alterations as risk factors in cardiovascular disease. Fundamental & Clinical Pharmacology 24(6): 665-674. https://doi.org/10.1111/j.1472-8206.2010.00860.x [PubMed]
■ Stühlinger MC, Tsao PS, Her JH, Kimoto M, Balint RF, Cooke JP (2001) Homocysteine impairs the nitric oxide synthase pathway: role of asymmetric dimethylarginine. Circulation 104(21): 25692575. https://doi.org/10.1161/hc4601.098514 [PubMed]
■ Su JB (2014) Different cross-talk sites between the renin-an-giotensin and the kallikrein-kinin systems. Journal of the Re-nin-Angiotensin-Aldosterone System 15(4): 319-328. https://doi. org/10.1177/1470320312474854 [PubMed]
■ Su JB (2015) Vascular endothelial dysfunction and pharmacological treatment. World Journal of Cardiology 7(11): 719-741. https://doi. org/10.4330/wjc.v7.i11.719 [PMC] [PubMed]
■ Su JB, Cazorla O, Blot S, Blanchard-Gutton N, Ait Mou Y, Barthélémy I, Sambin L, Sampedrano CC, Gouni V, Unterfinger Y, Aguilar P, Thibaud JL, Bizé A, Pouchelon JL, Dabiré H, Ghaleh B, Berdeaux A, Chetboul V, Lacampagne A, Hittinger L (2012) Bradykinin restores left ventricular function, sarcomeric protein phosphorylation, and e/nNOS levels in dogs with Duchenne
muscular dystrophy cardiomyopathy. Cardiovascular Research 95(1): 86-96. https://doi.org/10.1093/cvr/cvs161 [PubMed]
■ Sun H, Zhang H, Li K, Wu H, Zhan X, Fang F, Qin Y, Wei Y (2019) ESM-1 promotes adhesion between monocytes and endothelial cells under intermittent hypoxia. Journal of Cellular Physiology 234(2): 1512-1521. https://doi.org/10.1002/jcp.27016 [PubMed]
■ Sun HJ, Wu ZY, Nie XW, Bian JS (2020) Role of endothelial dysfunction in cardiovascular diseases: The Link between inflammation and hydrogen sulfide. Frontiers in Pharmacology 10: 1568. https:// doi.org/10.3389/fphar.2019.01568 [PMC] [PubMed]
■ Szmitko PE, Wang CH, Weisel RD, de Almeida JR, Anderson TJ, Verma S (2003) New markers of inflammation and endothelial cell activation: Part I. Circulation 108(16): 1917-1923. https://doi. org/10.1161/01.CIR.0000089190.95415.9F [PubMed]
■ Szydelko J, Trojanowska P, D^browska I, Szydelko-Gorzkowicz M, Litwinczuk M (2020) Adiponectin as novel biomarker of endothelial dysfunction in insulin resistance and obesity - a narrative review. Journal of Education, Health and Sport 10(9): 591-606. https://doi. org/10.12775/JEHS.2020.10.09.071
■ Takeda E, Suzuki Y, Sato Y (2016) Age-associated downregulation of vasohibin-1 in vascular endothelial cells. Aging Cell 15(5): 885892. https://doi.org/10.1111/acel.12497 [PMC] [PubMed]
■ Tarafdar A, Pula G (2018) The Role of NADPH oxidases and ox-idative stress in neurodegenerative disorders. International Journal of Molecular Sciences 19(12): 3824. https://doi.org/10.3390/ ijms19123824 [PMC] [PubMed]
■ Title LM, Cummings PM, Giddens K, Nassar BA (2000) Oral glucose loading acutely attenuates endothelium-dependent vasodilation in healthy adults without diabetes: an effect prevented by vitamins C and E. Journal of the American College of Cardiology 36(7): 21852191. https://doi.org/10.1016/S0735-1097(00)00980-3 [PubMed]
■ Tomasova L, Pavlovicova M, Malekova L, Misak A, Kristek F, Gr-man M, Cacanyiova S, Tomasek M, Tomaskova Z, Perry A, Wood ME, Lacinova L, Ondrias K, Whiteman M (2015) Effects of AP39, a novel triphenylphosphonium derivatised anethole dithiolethione hydrogen sulfide donor, on rat haemodynamic parameters and chloride and calcium Cav3 and RyR2 channels. Nitric Oxide 46: 131-144. https://doi.org/10.1016/j.niox.2014.12.012 [PubMed]
■ Tomiyama H, Higashi Y, Takase B, Node K, Sata M, Inoue T, Ishibashi Y, Ueda S, Shimada K, Yamashina A (2011) Relationships among hyperuricemia, metabolic syndrome, and endothelial function. American Journal of Hypertension 24(7): 770-774. https://doi. org/10.1038/ajh.2011.55 [PubMed]
■ Tonduangu D, Hittinger L, Ghaleh B, Le Corvoisier P, Sambin L, Champagne S, Badoual T, Vincent F, Berdeaux A, Crozatier B, Su JB (2004) Chronic infusion of bradykinin delays the progression of heart failure and preserves vascular endothelium-mediated vasodi-lation in conscious dogs. Circulation 109(1): 114—119. https://doi. org/10.1161/01.CIR.0000105726.89770.35 [PubMed]
■ Tong X, Lv P, Mathew AV, Liu D, Niu C, Wang Y, Ji L, Li J, Fu Z, Pan B, Pennathur S, Zheng L, Huang Y (2014) The compensatory enrichment of sphingosine -1-phosphate harbored on glycated high-density lipoprotein restores endothelial protective function in type 2 diabetes mellitus. Cardiovascular Diabetology 13: 82. https:// doi.org/10.1186/1475-2840-13-82 [PMC] [PubMed]
■ Topal G, Brunet A, Millanvoye E, Boucher JL, Rendu F, Devynck MA, David-Dufilho M (2004) Homocysteine induces oxidative stress by uncoupling of NO synthase activity through reduction of tetrahy-
drobiopterin. Free Radical Biology & Medicine 36(12): 1532-1541. https://doi.org/10.1016/j.freeradbiomed.2004.03.019 [PubMed]
■ Torzewski M, Rist C, Mortensen RF, Zwaka TP, Bienek M, Wal-tenberger J, Koenig W, Schmitz G, Hombach V, Torzewski J (2000) C-reactive protein in the arterial intima: role of C-reactive protein receptor-dependent monocyte recruitment in atherogenesis. Arteriosclerosis, Thrombosis, and Vascular Biology 20(9): 2094-2099. https://doi.org/10.1161/01.ATV.20.9.2094 [PubMed]
■ Tousoulis D, Georgakis MK, Oikonomou E, Papageorgiou N, Zaro-mitidou M, Latsios G, Papaioannou S, Siasos G (2015) Asymmetric dimethylarginine: clinical significance and novel therapeutic approaches. Current Medicinal Chemistry 22(24): 2871-2901. https:// doi.org/10.2174/0929867322666150625095046 [PubMed]
■ Toya T, Sara JD, Corban MT, Taher R, Godo S, Herrmann J, Le-rman LO, Lerman A (2020) Assessment of peripheral endotheli-al function predicts future risk of solid-tumor cancer. European Journal of Preventive Cardiology 27(6): 608-618. https://doi. org/10.1177/2047487319884246 [PubMed]
■ Traber MG, Stevens JF (2011) Vitamins C and E: beneficial effects from a mechanistic perspective. Free Radical Biology & Medicine 51(5): 1000-1013. https://doi.org/10.1016Zj.freerad-biomed.2011.05.017 [PMC] [PubMed]
■ Trask AJ, Ferrario CM (2007) Angiotensin-(1-7): pharmacology and new perspectives in cardiovascular treatments. Cardiovascular Drug Reviews 25(2): 162-174. https://doi.org/10.1111/j.1527-3466.2007.00012.x [PubMed]
■ Tseng YL, Chiang ML, Huang TF, Su KP, Lane HY, Lai YC (2010) A selective serotonin reuptake inhibitor, citalopram, inhibits collagen-induced platelet aggregation and activation. Thrombosis Research 126(6): 517-523. https://doi.org/10.1016/j.throm-res.2010.09.017 [PubMed]
■ Vairappan B (2015) Endothelial dysfunction in cirrhosis: Role of inflammation and oxidative stress. World Journal of Hepatology 7(3): 443-459. https://doi.org/10.4254/wjh.v7.i3.443 [PMC] [PubMed]
■ van den Berg BM, Nieuwdorp M, Stroes ES, Vink H (2006) Glyco-calyx and endothelial (dys) function: from mice to men. Pharmacological Reports 58(Suppl): 75-80. [PubMed]
■ van der Giet M, Tölle M, Kleuser B (2008) Relevance and potential of sphingosine-1-phosphate in vascular inflammatory disease. Biological Chemistry 389(11): 1381-1390. https://doi.org/10.1515/ BC.2008.165 [PubMed]
■ van Zyl LT, Lesperance F, Frasure-Smith N, Malinin AI, Atar D, Lal-iberte MA, Serebruany VL (2009) Platelet and endothelial activity in comorbid major depression and coronary artery disease patients treated with citalopram: the Canadian Cardiac Randomized Evaluation of Antidepressant and Psychotherapy Efficacy Trial (CREATE) biomarker sub-study. Journal of Thrombosis and Thrombolysis 27(1): 48-56. https://doi.org/10.1007/s11239-007-0189-3 [PubMed]
■ Varga Z, Flammer AJ, Steiger P, Haberecker M, Andermatt R, Zinkernagel AS, Mehra MR, Schuepbach RA, Ruschitzka F, Moch H (2020) Endothelial cell infection and endotheliitis in COVID-19. Lancet 395(10234): 1417-1418. https://doi.org/10.1016/S0140-6736(20)30937-5 [PMC] [PubMed]
■ Varin R, Mulder P, Tamion F, Richard V, Henry JP, Lallemand F, Le-rebours G, Thuillez C (2000) Improvement of endothelial function by chronic angiotensin-converting enzyme inhibition in heart failure: role of nitric oxide, prostanoids, oxidant stress, and bradykinin. Circulation 102(3): 351-356. https://doi.org/10.1161/01.CIR.102.3.351 [PubMed]
■ Vasina LV, Petrishchev NN, Vlasov TD (2017) Markers of endothelial dysfunction. Regional Circulation and Microcirculation [Region-arnoe Krovoobrashchenie i Mikrocirkulyatsiya] 16(1): 4-15. https:// doi.org/10.24884/1682-6655-2017-16-1-4-15 [in Russian]
■ Velena A, Zarkovic N, Gall Troselj K, Bisenieks E, Krauze A, Poi-kans J, Duburs G (2016) 1,4-Dihydropyridine derivatives: dihydron-icotinamide analogues-model compounds targeting oxidative stress. Oxidative Medicine and Cellular Longevity 2016: 1892412. https:// doi.org/10.1155/2016/1892412 [PMC] [PubMed]
■ Vera R, Sánchez M, Galisteo M, Villar IC, Jimenez R, Zarzuelo A, Pérez-Vizcaíno F, Duarte J (2007) Chronic administration of genistein improves endothelial dysfunction in spontaneously hypertensive rats: involvement of eNOS, caveolin and calmodulin expression and NADPH oxidase activity. Clinical Science 112(3): 183-191. https://doi.org/10.1042/CS20060185 [PubMed]
■ Villano A, Di Franco A, Nerla R, Sestito A, Tarzia P, Lamendola P, Di Monaco A, Sarullo FM, Lanza GA, Crea F (2013) Effects of ivabradine and ranolazine in patients with microvascular angina pec-toris. The American Journal of Cardiology 112(1): 8-13. https://doi. org/10.1016/j.amjcard.2013.02.045 [PubMed]
■ Virdis A, Iglarz M, Neves MF, Touyz RM, Rozen R, Schiffrin EL (2003) Effect of hyperhomocystinemia and hypertension on endotheli-al function in methylenetetrahydrofolate reductase-deficient mice. Arteriosclerosis, Thrombosis, and Vascular Biology 23(8): 1352-1357. https://doi.org/10.1161/01.ATV.0000083297.47245.DA [PubMed]
■ Vyssoulis GP, Marinakis AG, Aznaouridis KA, Karpanou EA, Arapogianni AN, Cokkinos DV, Stefanadis CI (2004) The impact of third-generation beta-blocker antihypertensive treatment on en-dothelial function and the prothrombotic state: effects of smoking. American Journal of Hypertension 17(7): 582-589. https://doi. org/10.1016/j.amjhyper.2004.03.668 [PubMed]
■ Wagner L, Laczy B, Tamaskó M, Mazák I, Markó L, Molnár GA, Wagner Z, Mohás M, Cseh J, Fekete A, Wittmann I (2007) Cigarette smoke-induced alterations in endothelial nitric oxide synthase phosphorylation: role of protein kinase C. Endothelium: Journal of Endothelial Cell Research 14(4-5): 245-255. https://doi. org/10.1080/10623320701606707 [PubMed]
■ Wang R, Szabo C, Ichinose F, Ahmed A, Whiteman M, Papapet-ropoulos A (2015) The role of H2S bioavailability in endothelial dysfunction. Trends in Pharmacological Sciences 36(9): 568-578. https://doi.org/10.1016Zj.tips.2015.05.007 [PubMed]
■ Webb GD, Lim LH, Oh VM, Yeo SB, Cheong YP, Ali MY, El Oakley R, Lee CN, Wong PS, Caleb MG, Salto-Tellez M, Bhatia M, Chan ES, Taylor EA, Moore PK (2008) Contractile and vasorelax-ant effects of hydrogen sulfide and its biosynthesis in the human internal mammary artery. The Journal of Pharmacology and Experimental Therapeutics 324(2): 876-882. https://doi.org/10.1124/ jpet.107.133538 [PubMed]
■ Werner N, Kosiol S, Schiegl T, Ahlers P, Walenta K, Link A, Böhm M, Nickenig G (2005) Circulating endothelial progenitor cells and cardiovascular outcomes. The New England Journal of Medicine 353(10): 999-1007. https://doi.org/10.1056/NEJMoa043814 [PubMed]
■ Westermann D, Riad A, Richter U, Jäger S, Savvatis K, Schuchardt M, Bergmann N, Tölle M, Nagorsen D, Gotthardt M, Schultheiss HP, Tschöpe C (2009) Enhancement of the endothelial NO synthase attenuates experimental diastolic heart failure. Basic Research in Cardiology 104(5): 499-509. https://doi.org/10.1007/s00395-009-0014-6 [PubMed]
■ Wilkerson BA, Argraves KM (2014) The role of sphingosine-1-phos-phate in endothelial barrier function. Biochimica et Biophysica Acta 1841(10): 1403-1412. https://doi.org/10.1016/j.bbalip.2014.06.012 [PMC] [PubMed]
■ Wong MS, Delansorne R, Man RY, Svenningsen P, Vanhoutte PM (2010) Chronic treatment with vitamin D lowers arterial blood pressure and reduces endothelium-dependent contractions in the aorta of the spontaneously hypertensive rat. American Journal of Physiology. Heart and Circulatory Physiology 299(4): H1226-H1234. https:// doi.org/10.1152/ajpheart.00288.2010 [PubMed]
■ Wong MS, Delansorne R, Man RY, Vanhoutte PM (2008) Vitamin D derivatives acutely reduce endothelium-dependent contractions in the aorta of the spontaneously hypertensive rat. American Journal of Physiology. Heart and Circulatory Physiology 295(1): H289-H296. https://doi.org/10.1152/ajpheart.00116.2008 [PubMed]
■ Xuan C, Chang FJ, Liu XC, Bai XY, Liao XL, He GW, Ou JS (2012) Endothelial nitric oxide synthase enhancer for protection of endo-thelial function from asymmetric dimethylarginine-induced injury in human internal thoracic artery. The Journal of Thoracic and Cardiovascular Surgery 144(3): 697-703. https://doi.org/10.1016/j. jtcvs.2012.01.020 [PubMed]
■ Xue HM, Yu CM, Underwood MJ, Huang JH, Yang Q (2012) AVE3085 protects coronary endothelium from the impairment of asymmetric dimethylarginine by activation and recoupling of eNOS. Cardiovascular Drugs and Therapy 26(5): 383-392. https://doi. org/10.1007/s10557-012-6404-2 [PubMed]
■ Yamaguchi Y, Haginaka J, Morimoto S, Fujioka Y, Kunitomo M (2005) Facilitated nitration and oxidation of LDL in cigarette smokers. European Journal of Clinical Investigation 35(3): 186-193. https://doi.org/10.1111/j.1365-2362.2005.01472.x [PubMed]
■ Yang G, Wu L, Jiang B, Yang W, Qi J, Cao K, Meng Q, Mustafa AK, Mu W, Zhang S, Snyder SH, Wang R (2008) H2S as a physiologic vasorelaxant: hypertension in mice with deletion of cystathionine gamma-lyase. Science 322(5901): 587-590. https://doi.org/10.1126/ science.1162667 [PMC] [PubMed]
■ Yang Q, Xue HM, Wong WT, Tian XY, Huang Y, Tsui SK, Ng PK, Wohlfart P, Li H, Xia N, Tobias S, Underwood MJ, He GW (2011) AVE3085, an enhancer of endothelial nitric oxide synthase, restores endothelial function and reduces blood pressure in spontaneously hypertensive rats. British Journal of Pharmacology 163(5): 1078-1085. https://doi.org/10.1111/j.1476-5381.2011.01308.x [PMC] [PubMed]
■ Yang X, Gu J, Lv H, Li H, Cheng Y, Liu Y, Jiang Y (2019) Uric acid induced inflammatory responses in endothelial cells via up-reg-ulating(pro)renin receptor. Biomedicine & Pharmacotherapy 109: 1163-1170. https://doi.org/10.1016/j.biopha.2018.10.129 [PubMed]
■ Yang X, Yu Y, Xu J, Shu H, Xia J, Liu H, Wu Y, Zhang L, Yu Z, Fang M, Yu T, Wang Y, Pan S, Zou X, Yuan S, Shang Y (2020) Clinical course and outcomes of critically ill patients with SARS-CoV-2 pneumonia in Wuhan, China: a single-centered, retrospective, observational study. The Lancet. Respiratory Medicine 8(5): 475-481. https://doi.org/10.1016/S2213-2600(20)30079-5 [PMC] [PubMed]
■ Yasu T, Kobayashi M, Mutoh A, Yamakawa K, Momomura S, Ueda S (2013) Dihydropyridine calcium channel blockers inhibit non-esterified-fatty-acid-induced endothelial and rheological dysfunction. Clinical Science 125(5): 247-255. https://doi.org/10.1042/ CS20120311 [PubMed]
■ Yu HS, Lin TH, Tang CH (2013) Bradykinin enhances cell migration in human prostate cancer cells through B2 receptor/PKCS/c-Src
dependent signaling pathway. Prostate 73(1): 89-100. https://doi. org/10.1002/pros.22544 [PubMed]
■ Yuan S, Patel RP, Kevil CG (2015) Working with nitric oxide and hydrogen sulfide in biological systems. American Journal of Physiology. Lung Cellular and Molecular Physiology 308(5): L403-L415. https://doi.org/10.1152/ajplung.00327.2014 [PMC] [PubMed]
■ Zepeda RJ, Castillo R, Rodrigo R, Prieto JC, Aramburu I, Bruge-re S, Galdames K, Noriega V, Miranda HF (2012) Effect of carve-dilol and nebivolol on oxidative stress-related parameters and endothelial function in patients with essential hypertension. Basic & Clinical Pharmacology & Toxicology 111(5): 309-316. https://doi. org/10.1111/j.1742-7843.2012.00911.x [PubMed]
■ Zhang JG, Wang LZ, Han XQ, Jiang YD, Zhang RM, Wang SR (2007) The pathogenic mechanism of homocysteine-induced endothelial nitric oxide synthase dysfunction and the antagonistic effects by folic acid. Journal of Molecular Cell Biology 40(1): 17-23. [PubMed]
■ Zhang MY, Dugbartey GJ, Juriasingani S, Sener A (2021) Hydrogen sulfide metabolite, sodium thiosulfate: clinical applications and underlying molecular mechanisms. International Journal of Molecular Sciences 22(12): 6452. https://doi.org/10.3390/ijms22126452 [PubMed]
■ Zhang S, Zhang S, Wang H, Wu W, Ye Y (2017) Arginine methyla-tion dysfunction increased risk of acute coronary syndrome in coronary artery disease population. Medicine 96(7): e6074. https://doi. org/10.1097/MD.0000000000006074 [PubMed][PMC]
■ Zhang SM, Zuo L, Zhou Q, Gui SY, Shi R, Wu Q, Wei W, Wang Y (2012) Expression and distribution of endocan in human tissues. Biotechnic & Histochemistry 87(3): 172-178. https://doi.org/10.310 9/10520295.2011.577754 [PubMed]
■ Zhang X, Li H, Jin H, Ebin Z, Brodsky S, Goligorsky MS (2000) Effects of homocysteine on endothelial nitric oxide production. American Journal of Physiology. Renal Physiology 279(4): F671-F678. https://doi.org/10.1152/ajprenal.2000.279.4.F671 [PubMed]
■ Zhang X, Sessa WC, Fernandez-Hernando C (2018) Endothelial transcytosis of lipoproteins in atherosclerosis. Frontiers in Cardiovascular Medicine 5: 130. https://doi.org/10.3389/fcvm.2018.00130 [PMC] [PubMed]
■ Zhang Y, Lu J, Shi J, Lin X, Dong J, Zhang S, Liu Y, Tong Q (2008) Central administration of angiotensin-(1-7) stimulates nitric oxide
release and upregulates the endothelial nitric oxide synthase expression following focal cerebral ischemia/reperfusion in rats. Neuropeptides 42(5-6): 593-600. https://doi.org/10.1016Zj.npep.2008.09.005 [PubMed]
■ Zhang Z, Gu X, Fang X, Tang Z, Guan S, Liu H, Wu X, Wang C, Zhao Y (2020) Homocysteine and the risk of cardiovascular events and all-cause death in elderly population: A community-based prospective cohort study. Therapeutics and Clinical Risk Management 16: 471-481. https://doi.org/10.2147/TCRM.S239496 [PMC] [PubMed]
■ Zhao W, Zhang J, Lu Y, Wang R (2001) The vasorelaxant effect of H2S as a novel endogenous gaseous K(ATP) channel opener. The EMBO Journal 20(21): 6008-6016. https://doi.org/10.1093/em-boj/20.21.6008 [PubMed] [PMC]
■ Zhen H, Gui F (2017) The role of hyperuricemia on vascular endothelium dysfunction. Biomedical Reports 7(4): 325-330. https://doi. org/10.3892/br.2017.966 [PMC] [PubMed]
■ Zhen P, Zhao Q, Hou D, Liu T, Jiang D, Duan J, Lu L, Wang W (2012) Genistein attenuates vascular endothelial impairment in ova-riectomized hyperhomocysteinemic rats. Journal of Biomedicine & Biotechnology 2012: 730462. https://doi.org/10.1155/2012/730462 [PMC] [PubMed]
■ Zhou MS, Tian R, Jaimes EA, Raij L (2014) Combination therapy of amlodipine and atorvastatin has more beneficial vascular effects than monotherapy in salt-sensitive hypertension. American Journal of Hypertension 27(6): 873-880. https://doi.org/10.1093/ajh/hpt272 [PubMed]
■ Ziccardi P, Nappo F, Giugliano G, Esposito K, Marfella R, Cioffi M, D'Andrea F, Molinari AM, Giugliano D (2002) Reduction of inflammatory cytokine concentrations and improvement of endothelial functions in obese women after weight loss over one year. Circulation 105(7): 804-809. https://doi.org/10.1161/hc0702.104279 [PubMed]
■ Zisman LS, Keller RS, Weaver B, Lin Q, Speth R, Bristow MR, Canver CC (2003) Increased angiotensin-(1-7)-forming activity in failing human heart ventricles: evidence for upregulation of the angiotensin-con-verting enzyme Homologue ACE2. Circulation 108(14): 1707-1712. https://doi.org/10.1161/01.CIR.0000094734.67990.99 [PubMed]
Author contributions
■ Dmitry V. Shcheblykin, post-graduate student, Department of Pharmacology and Clinical Pharmacology; e-mail: [email protected], ORCID ID https://orcid.org/0000-0002-2420-2243. The author was engaged in literature analysis and paper writing.
■ Anton A. Bolgov, post-graduate student, Department of Pathology; e-mail: [email protected], ORCID ID https://orcid.org/0000-0001-9708-8181. The author made a contribution to collection, analysis and interpretation of the data for the paper.
■ Mikhail V. Pokrovskii, Doctor Habil. of Medical Sciecnes, Professor, Department of Pharmacology and Clinical Pharmacology, e-mail: [email protected], ORCID ID https://orcid.org/0000-0002-1493-3376. The author set the research objectives, motivated the team, conducted a critical analysis of the material, and gave the final approval of the version of the manuscript.
■ Julia V. Stepenko, postgraduate student, Department of Pharmacology and Clinical Pharmacology, e-mail: [email protected], ORCID ID http://orcid.org/0000-0002-7414-7326. The author was engaged in structuring the article and arranging the references.
■ Julia M. Tsuverkalova, postgraduate student, Department of Pharmacology and Clinical Pharmacology, e-mail: [email protected], ORCID ID http://orcid.org/0000-0001-8489-247X. The author was engaged in structuring the article and arranging the references.
■ Olesya V. Shcheblykina, PhD in Medical Sciences, senior lecturer, Department of Pharmacology and Clinical Pharmacology, e-mail: [email protected], ORCID ID https://orcid.org/0000-0003-0346-9835. The author was engaged in developing the concept and conducting the literature analysis.
■ Polina A. Golubinskaya, PhD in Medical Sciences, Head of the Clinical Diagnostic Laboratory, Budgetary Healthcare Institution of the Voronezh Region "Voronezh Regional Clinical Ophthalmological Hospital", e-mail: [email protected], ORCID ID http://orcid.org/0000-0002-1765-9042. The author was engaged in collecting information and writing the article.
■ Liliya V. Korokina, PhD in Medical Sciences, Associate Professor of the Department of Pharmacology and Clinical Pharmacology, e-mail: [email protected], ORCID ID http://orcid.org/0000-0002-4115-1564. The author was engaged in developing the concept and conducting the literature analysis.
