CC BY
39
Electrocatalytic Oxidation and Sensitive Determination of N-Acetyl-L-Cysteine at Cyclodextrin-Carbon Nanotubes Modified Glassy Carbon Electrode
Jun Zhangab, Yan Changa and Chuan Donga
"Institute of Environmental Science, Shanxi University, Taiyuan 030006, PR China, e-mail: dc104@sxu.edu.cn bNational and Local Joint Engineering Laboratory of Traffic Civil Engineering Materials, Chongqing Jiaotong University, Chongqing 400074, PR China
The electrochemical behavior and electroanalysis of N-acetyl-L-cysteine (NAC) were studied at a glassy carbon electrode modified with P-cyclodextrin (P-CD) and carbon nanotubes (P-CD/MWCNT/GCE). The P-CD/MWCNT/GCE displayed excellent electrocatalytic performance to the catalytic oxidation reactions of NAC. Because NAC is selectively enriched by P-CD, the P-CD/MWCNT/GCE can solve the problems of the signal attenuation caused by the adsorption of the NAC oxidation product. The influence of the experimental conditions on the NAC electrochemical behavior was also considered, when using a modified electrode. The mechanism and kinetics of the catalytic oxidation reactions of NAC were monitored by the cyclic voltammetry and chronoampero-metry. The catalytic oxidation rate constant k (4.21±0.05)x103 M-1S-1 was calculated using electrochemical approaches. The results showed that in 1.0*10-3 M of potassium ferricyanide solution, the current signals were proportional to the NAC concentration from 4.4x10-4 M to 8.0x10-2 M [IP(10-6A) = = 0.58412 + 5.38x103 c(M), R2 = 0.9934], and the detection limit (S/N = 3) was 5.02x10-5 M. For 80 mM NAC, six successive measurements yielded R.S.D. of 3.4%, which shows that the sensor is reproducible. The proposed method can be applied for the determination of NAC in routine analysis.
Keywords: chemical modified electrode, carbon nanotubes, cyclodextrin, N-Acetyl-L-cysteine, electrochemical determination.
УДК 543.08
INTRODUCTION
As a glutathione precursor, N-Acetyl-L-Cysteine (NAC) is an important mercapto supplying agent, which plays crucial roles in the anti-apoptotic drugs, being an antitoxic, anti-oxidating agent that elimi-nats free radicals and prevents radiation damage effects. Traditionally, NAC is used in the treatment of respiratory tract diseases, but recently it has been also found superior to the treatment of cancer, AIDS and some other grave diseases [1]. The determination of NAC has received considerable attention due to its pharmaceutical and clinical importance in such areas as the protein characterization, analysis of commercial tablets, and injection samples. Commonly used methods for the determination of NAC include high performance liquid chromatography [2], iodometric titration [3], chemiluminescence [4], and electrochemical method. However, many of them are insufficiently sensitive, time consuming and employed in expensive instrumentations. Compared to other options, an electro-analysis has the advantages of being highly sensitive and easy to use. Therefore, it is timely to explore and develop a simple and reliable electrochemical method for the determination of NAC.
Currently, the electrocatalytic oxidation of NAC has attracted much interest of researchers because of its high overpotential on solid electrodes, such as glassy carbon electrodes (GCEs) and metal electrodes [5, 6]. Nowadays various modified electrodes are used in the electrochemical studies on NAC oxidation including a copper ferrocyanide modified electrode [7], Fe2O3-CoHCF modified electrode [8], and a pyrroloquinoline quinone modified electrode [9]. Moreover, the electrolyte K3Fe(CN)6 is found to act as electron transfer medium in the electrocatalytic oxidation at a carbon paste electrode [10-12]. In our previous paper, we reported a near-infrared luminescence quenching method for the detection of phenolic compounds using the NAC-protected gold nanoparticles-tyrosinase hybrid material [13].
Recently, cyclodextrin and its derivatives have been widely used in the design and synthesis of the molecule selective chemical sensors. In addition, electrochemical sensors based on cyclodextrins and their inclusion complexes have been applied in the electrochemical synthesis, batteries and anticorrosion chemistry. For instance, El-Hady [14] designed a hydroxypropyl-P-cyclodextrin modified carbon paste sensor applied successfully to the determination of catechins. Lee et al. [15] coated the
© Jun Zhang, Yan Chang, Chuan Dong, Электронная обработка материалов, 2015, 51(2), 8-15.
surface of the sensing element with a layer of a-cyclodextrin for the tyrosine detection.
It is well known that multiwalled carbon nano-tubes (MWCNT) have excellent performance in promoting the electron transfer when they are used as electrodes in electrochemical reactions. However, if the carbon nanotubes are assembled with cy-clodextrins, the result should be a new material simultaneously possessing unique properties of carbon nanotubes and cyclodextrins, which will provide excellent opportunities for applications as sensors [16]. It is know from literature that a P-cyclodextrin incorporated carbon nanotube modified electrode has been proposed for the determination of norepi-nephrine [17], of adenine, guanine, and thymine [18], of rutin [19], as well as for identification of cancer cells [20]. Karimi et al. [21-24] developed several sensors for voltammetric determination of NAC using a modified MWCNT electrode. In our previous studies, MWCNTs/Nafion modified GCE and its interaction with cyclodextrins were proved to be good for the electrochemical behavior of several dyes [25, 26]. Still, to the best of our knowledge, the electrocatalytic oxidation and electroanalytical methods of NAC at cyclodextrin-carbon nanotube modified GCEs in the potassium ferricyanide electrolyte still have not been reported so far.
In the present paper, a GCE was modified with MWCNT and a P-cyclodextrin (P-CD) composite. The modified electrode was used as the working electrode for the electro-catalytic oxidation of NAC. In addition, a novel, simple and sensitive electrochemical method with K3Fe(CN)6 as the catalyst for the determination of NAC is described and specified. This method overcomes the contamination of the electrode interface from the oxidation product of NAC and makes it possible to achieve high sensitivity and selectivity.
EXPERIMENTAL
Instruments and Reagents
All chemicals were purchased and used as received without any purification; aqueous solutions were prepared with doubly distilled water. MWCNTs (purity >90%) P-CD were obtained from Chinese enterprises. All electrochemical experiments were performed on a CHI660D electrochemical workstation (Chinese make) with a conventional three-electrode cell. A bare or modified GCE was used as working electrode. An Ag/AgCl electrode and a platinum wire were used as reference electrode and auxiliary electrode, respectively. A pH-meter (Corning, Model 140) with a double junction glass electrode was used to check the pH of the solutions.
Preparation of Electrode
A GCE was polished, in turn, with 1.0, 0.3 and 0.05 ^m alumina powders on micro-cloth and then thoroughly cleaned ultrasonically with ethanol and double distilled water. MWCNT (1mg) was dispersed in 0.5 mL aqueous solution of P-CD (0.1M) with the aid of ultrasonication to give a stable black MWCNT suspension. Then 3 ^L of the mixture were cast onto the surface of the GCE and then dried with an infrared lamp. Afterwards, a drop of 0.05% alcohol diluted nafion solution was spread onto the MWCNT/P-CD coated electrode to improve the permeability and stability of the film. Besides, the P-CD/GCE and MWCNT/GCE were prepared in the same way to be used for comparison purposes.
RESULTS AND DISCUSSION
Electrocatalytic oxidation of NAC with K3Fe(CN)6
In a potential range from -0.20 to 0.70V, the representative cyclic voltammograms of GCE with and without NAC or/and K3Fe(CN)6 were obtained in PBS containing 0.20M Na2SO4 aqueous solution. Curve a in Fig. 1 is the CV of the base solution, whereas at the cyclic voltammogram of the supporting electrolyte at the P-CD/MWCNT/GCE neither anodic nor cathode peaks appeared. Curve b in Fig. 1 shows that when NAC was added into the base solution, the oxidation peak current increased gradually with the scan progresses, and an ill-defined oxidation peak was displayed. This anodic current probably resulted from a weak interaction of carbon and sulfur atoms. Curve c in Fig. 1 is the cyclic voltammeter graph of 1.0x10"3 M K3Fe(CN)6 in the buffer solution system.
Curve d in Fig. 1 shows that when K3Fe(CN)6 was added to the buffer solution system containing NAC, an obvious irreversible catalytic oxidation peak appeared at about 0.2 V and the oxidation peak current significantly increased with the scan progresses, indicating good electrocatalytic properties of K3Fe(CN)6 to the oxidation of NAC, when working with Na2SO4 as electrolyte at P-CD/MWCNT/GCE. In addition, the almost disappeared cathode peak current also indicates a strong catalytic effect.
The differential pulse voltammetry (DPV) was used to study the electrochemical behavior of NAC at the P-CD/MWCNT/GCE. As shown in Fig. 2, when the concentration of NAC increased, the oxidation peak current was found to gradually increase, suggesting a good electrochemical response of the modified electrode to NAC. However, the oxidation
potential was found to shift negatively, which may be due to the accumulation of an oxidized product of NAC on the electrode surface.
0.00020
0.00015
< 0.00010
c o H 0.00005
3
u
0.00000
-0.00005
-0.00010
a......Buffer
- b-K3[Fe(CN)6]+NAC
- c.....K3[Fe(CN)6]
. d-Buffer+NAC
d/"--
a
i i
0.4
0.6
0.8
-0.2 0.0 0.2
Potential, V vs Ag/AgC1
Fig. 1. CVs of (a) PBS; (b) PBS+8x10"2 NAC; (c) PBS+ 1.0x10"3 M K3Fe(CN)6; (d) PBS+8x10-2 NAC+1.0x10-3 M K3Fe(CN)6 at ß-CD/MWCNT/GCE. Scan rate: 50 mV/s.
0.00025
0.00020
0.00015
s
<u b
ß 0.00010
0.00005
0.00000 -
- T o ~ 1.6
- f-7 e—jr. ^ 1.4 *—d i,2 / y-1.2233KI.(li2X y/ RJ-0.9S71
■A-c 4—b 0 2 4 S S 10 Conc.NAC, * ¡0"4 M
- -A— a
..........
-0.2
0.0
0.2 0.4 Potential, V
0.6
O.i
Fig. 2. DPVs of ß-CD/MWCNT/GCE in buffer, for different concentration of NAC(x10-4 M) (a) 0; (b) 3.2; (c) 6.4; (d) 8.0; (e) 8.8; (f) 9.6. Scan rate: 100 mV/s. Inset: Plot of electrocataly-tic peak currents vs. NAC concentration.
0 100 200 300 400
Time, s
Fig. 3. Chronoamperograms of 1x10"3 M K3Fe(CN)6 in the absence (b) and presence (a) of 8x10-2 M NAC.
Catalytic Oxidation Rate Constant k
Chronoamperometry was used to study the mass transfer kinetics and to obtain heterogeneous catalytic rate constant. Under the selected experimental conditions, the potential of P-CD/MWCNT/GCE as sensors for the NAC measurement was evaluated by chronoamperometry, and the developed sensor was applied to an aqueous solution of K3Fe(CN)6 with or without NAC. The current-time curves of P-CD/MWCNT/GCE in the absence and presence of NAC are shown in Fig. 3, indicating a more significant electrocatalytic effect of K3Fe(CN)6 on the NAC oxidation at this electrode.
Because K3Fe(CN)6 can act as homogeneous mediator for the electro-oxidation of NAC, it should follow a chronoamperometric equation as below:
Ic/Il = X1/2[n1/2erf (X1/2) + exp (-X)/X1/2] (1)
where IC is the catalytic current in the presence of NAC, IL is the diffusion-limited current in the absence of NAC, and X = kc0t (k is the catalytic rate constant, c0 - the bulk initial concentration of NAC) is the argument of the error function. When X exceeds 1.5, the error function is almost equal to, the reaction zone is in the pure kinetic region, and the above equation can be reduced to
(2)
„0 A 1/2
T /r 1/2* 1/2 1/2/7 0A
ic/il = n X = n (kc t)
The plot of X vs. t can be given by combining Eq. (2) with the experimental data, and then one can simply calculate the value of the catalytic rate constant k from the slope (c0 = 8x10"2 M). The advantage of this method is that it is not necessary to know the diffusion coefficient or the electrode area. In this study, Ic and Il were measured at t > 55 ms, where Ic/Il > 1.5, and the catalytic oxidation rate constant k (4.21±0.05)x103 M"1S"1 was calculated. This value of the catalytic rate constant is near the value that was reported for the electrooxidation of NAC on a palladized aluminum electrode covered by the Prussian blue film [5]. The value of k explains well the sharp shape of the peak observed for the electrochemical oxidation of NAC in the presence of K3Fe(CN)6 on the surface of a GCE.
The pH of a solution can have more pronounced effects on the catalytic activity of K3Fe(CN)6. Therefore, the electrochemical behavior of K3Fe(CN)6 at the P-CD/MWCNT/GCE was investigated in the pH range from 1 to 10. The results show that the peak current decreases with the increase of the pH of the aqueous solution. It is an indicator of the participation of protons in the electrode reaction of K3Fe(CN)6 under these reaction conditions. The findings of the current study are consistent with
those of Jahan [11]. However, in order to eliminate the interference to NAC and improve the usefulness and feasibility of the proposed method, it is necessary to maintain the pH at normal physiological conditions (when pH is around 7). Therefore, the pH of 7.4 was chosen as optimum for the electrooxida-tion of NAC on the CD/MWCNT/GCE surface.
0.00020 -
-0.2 0.0 0.2 0.4 0.6 Potential, V
Fig. 4. DPVs of 3.2x10-4 M of NAC at bare GCE (a); P-CD/GCE (b); P-CD/MWCNT/GCE (c); MWCNT/GCE (d).
Comparison of the Coating on Modified Electrode
In a phosphate buffer solution with the pH of 7.4, differential pulse scans were registered from -0.2V to 0.7V for bare GCE, P-CD/GCE, MWCNT/GCE, and P-CD/MWCNT/GCE, respectively, and the results are shown in Fig. 4. Curve a in Fig. 4 shows the DPV of NAC at bare GCE, which exhibits the lowest oxidation current; for P-CD/GCE, a larger oxidation current and a slightly better peak shape are observed (curve b in Fig. 4). Curve d in Fig. 4 shows that an electrochemical response of MWCNT/GCE is really strong, but the peak shape is not well-defined and the signal decreases gradually in a continuous scan. The reasons for this phenomenon may be that the MWCNT interface layer plays an obvious role in the catalytic oxidation of NAC, but NAC and its oxidation products were irreversibly adsorbed on the carbonaceous material, by which the active sites of the electrode surface were plugged [27]. Obviously, a clear and distinct anodic peak with a relatively good peak shape in the DPV is displayed for P-CD/MWCNT/GCE (curve c in Fig. 4). This performance is relative to the combination of carbon nanotubes, and P-CD definitely improves the characteristics of the NAC oxidation.
Fig. 4 used in conjunctions with Fig. 2, where the oxidation potential shifted negatively, illustrates that the P-CD/MWCNT/GCE showed an excellent elec-trocatalytic effect on the oxidation of NAC. A high electrocatalytic efficiency of the P-CD/MWCNT/
GCE can be related to both the size and specific structure of the material. When P-CD is mixed with MWCNT in the case of ultrasonic agitation, they would be adsorbed and attached to the wall of MWCNT due to the steric hindrance and the binding force. Moreover, MWCNTs can be highly dispersed by P-CD, allowing a very uniform coating film to be formed on the electrode surface. The P-CD/MWCNT film has been evenly distributed on the surface of GCE. Therefore, MWCNTs are fully and easily accessible for NAC and, consequently, can be readily and completely used as electrochemical sensing units. The SEM and TEM images of the cyclodextrin-carbon nanotube composites show the structure of stereo-porous interspaces [20, 28, 29].
It is well known that MWCNTs have excellent catalytic effects owing to their high surface activity and electron conductivity. The roles of MWCNTs are: (i) to transfer electrons between the an electrode surface and NAC based on the intrinsic property of MWCNT; (ii) to increase the conductive area based on the high specific surface of MWCNT; (iii) to dope P-CD into the modified layer since P-CD cannot be easily modified at a bare GCE [28]. Furthermore, P-CD has a special cavity structure. When MWCNTs were modified on the surface of GCE, the rate of the electron transfer between NAC and electrode surfaces rose significantly and the electroca-talysis of NAC went up dramatically. In addition, there was a synergistic electrocatalysis between the P-CD and MWCNTs, which increases the oxidation current as a response of NAC. That is, the electron transfer rate between NAC and the electrode surface raises, and the electrocatalytic activity of NAC is improved by the P-CD-MWCNTs modified on the GCE surface.
Furthermore, curve c in Fig. 4 demonstrates that the signal intensity remains almost unchanged at continuous scanning. As is well known, the matching of the size and energy between P-CD and guest molecules is essential to the formation of an inclusion complex. The NAC molecule is well compatible with P-CD attributing it to a less steric hindrance and higher hydrophobicity of the mercapto group on the alkyl chain portion of the NAC molecule. That is to say, a NAC molecule penetrates deeply into the hydrophobic cavity of P-CD and a stable P-CD inclusion complex is formed. On the contrary, the molecular size of the oxidation product RS-SR is relatively large, and the hydrophilic groups -COOH and -NH2 are at the both ends of the molecule. Namely, the RS-SR molecule is a poor match for the P-CD in size and energy, so the inclusion complex cannot be formed.
In brief, NAC is selectively enriched by the P-CD attached at the surface of a carbon nanotube, which plays an important role of selective filtration membranes. As a consequence, it can be affirmatively concluded that P-CD/MWCNT/GCE with excellent performance including high electrocatalytic activity is a promising anode for the determination of NAC. Moreover, it is of particular significance that the P-CD/MWCNT/GCE could be applied to solve the problems of the signal attenuation caused by the adsorption of the NAC oxidation product on electrodes, in most cases.
A calibration curve of NAC
In order to develop a more simple and sensitive sensing procedure for the analysis of NAC, am-perometry technique was employed. As we know in chronoamperometry, a higher working potential tends to mean a larger anode current and a higher sensitivity. But at higher potentials, the interference from the oxidation of the coexisting substance is inevitable in real samples. Therefore, 0.214V was chosen as the potential of the working electrode for the determination of NAC. Fig. 5 shows the current response of P-CD/MWCNT/GCE to successive additions of 1x10"4 M NAC into a stirred PBS containing 0.20 M Na2SO4 and 1x10"3 M KsFe(CN)6 in a chronoamperometric experiment. It was found that after the concentration of NAC changes, the current attains the dynamic equilibrium 3 min later. The electrode response was quite rapid and proportional to the NAC concentration (Fig. 5, Inset).
Fig. 5. Performance of P-CD/MWCNT/GCE in amperometric detection of NAC (1x10-4 M) at 0.214V vs. Ag/AgCl. Inset: calibration curve for NAC concentrations between 0.5 mM and 3 mM.
Under the above-described optimum conditions, the determination of NAC at varying concentrations was performed at P-CD/MWCNT/GCE. The results
show that the anodic peak current is linear to the concentration of nAc from 4.4x10"4 M to 8.0x10"2 M. The linear regression equation is expressed as: ip = 0.58412 + 5.38x103 c, (ip (^A), c(M), R2 = 0.9934), and the detection limit for NAC is 5.02x10-5 M. Table 1 depicts a comparison between the present results with other electrochemical studies for the NAC oxidation. It is evident that P-CD/MWCNT/GCE is stable, with a wide linear range and low detection limit.
Table 1. Comparison of the present results with other electrochemical studies for NAC oxidation
Electrode Linearity Range (pM) Detection Limit (PM) Reference
Nanoparticles of iron (III) oxide core-cobalt hexacyanoferrate shell-modified carbon paste electrode 20-432 0.0209 [8]
Palladized aluminum electrode modified by Prussian blue film 2-40 0.54 [5]
Mercury Film Electrode 0.012-5.0 5000 [30]
GCE 200-4000 - [31]
10-Methylphenothiazine Modified Carbon Paste Electrode 1.1-1300 0.8 [32]
Multi-wall carbon nano-tubes modified GCE 100-10000 20 [33]
Carbon paste electrode 80-1000 60 [34]
Iron nitroprusside modified graphite paste electrode 60-14000 1050 [35]
P-CD/MW CNT/GCE 440-80000 50.2 This work
Recovery studies can show possible interferences from common excipients used in the pharmaceutical forms. To study the reproducibility and accuracy of the proposed analysis method, recovery experiments were carried out using the standard addition method. In order to find out whether the excipients show any interference with the analysis, the known amounts of pure NAC were added to the pre-analyzed pharmaceutical formulations and the mixtures were analyzed by the proposed method. The recoveries of NAC were calculated using the corresponding regression equations of the respective previously plotted calibration plots.
The accuracy of the method was checked by carrying out recovery studies. The modified P-CD/MWCNT/GCE electrode was applied to detect NAC in simulated samples of the PBS solution containing 0.20M Na2SO4 and 1x10-3 M K3Fe(CN)6. In
this procedure, 10 mM of the NAC standard were added to pre-analyzed samples containing 10.0 mM of NAC before determination by the recommended method. When the relative error was ±5%, the recoveries were approximately 94.7%-104.7% for the method, as shown in Table 2.
To evaluate the selectivity of the proposed electrochemical method, the interference effects of common ions, excipients in pharmaceutical preparations and common substances present in biological fluids were checked under the optimized conditions in a standard solution of 1.0x10-5 M NAC. The tolerance of each foreign species was taken as the largest concentration yielding less than ±5% of the error of the adoptive concentration of NAC. No interference could be found in the presence of 250-fold glucose, sucrose, starch, Na+, K+, Ca2+, Mg2+, Zn2+, SO42", PO43", NO3", acetic acid, propanone and ethanol. These results prove a good accuracy of the proposed method and the absence of interferences from common excipients, indicating that the proposed method can be reliably used for a routine analysis. Ease of application, sensitivity, short analysis time, low cost and reliability are its main advantages.
CONCLUSIONS
A CD/MWCNT/GC electrode has been applied to effectively catalyze the oxidation of NAC in the presence of K3Fe(CN)6 for its sensitive determination. A simple, rapid and economical electrochemical method for the determination of NAC has been successfully developed and validated with satisfactory results. The novel electrochemical sensor has been manufactured using the CD/MWCNT/GCE and the K3Fe(CN)6 medium. The results of the cyclic voltammetry implied that the CD/MWCNT/GCE is characterized by a high catalytic activity and fine stability, superior to other electrodes. The proposed electrode is reliable, simple, and rapid to prepare; it is low cost, precise, and does not require an extensive preliminary sample treatment. In conclusion, the electrochemical sensor has the advantages of good reproducibility and stability, and it is expected
to be used for clinical rapid analysis of the NAC detection.
ACKNOWLEDGMENTS
This work was supported by the National Natural Science Foundation of China (No: 21175086) and the Nature Science Foundation of Shanxi Province of China (No: 2013011010-3). All the authors express their deep thanks.
REFERENCES
1. Vries D.N. and Flora D.S. N-Acetyl-l-Cysteine. J Cell Biochem Suppl. 1993, 53(17F), 270-277.
2. Tsikas D., Sandmann J., Ikic M., Fauler J., Stichtenoth D.O. and Frolich J.C. Analysis of Cysteine and N-acety-L-cysteine in Human Plasma by Highperformance Liquid Chromatography at the Basal State and After Oral Administration of N-acetyl-cysteine. J Chromatogr B. Biomed Sci Appl. 1998, 708(1-2), 55-60.
3. Pharmacopoeia Commission of the Ministry of Health of the People's Republic of China. Pharmacopoeia of the People's Republic of China (2th Ed), Beijing: Chemical Industry Press, 2005. P. 4.
4. Toyooka T., Chokshi H.P., Givens R.S., Carlson R.G., Lunte S.M. and Kuwana T. Fluorescence and Chemiluminescence Detection of Oxazole-labelled Amines and Thiols. Biomed Chromatogr. 1993, 7(4), 208-216.
5. Pournaghi-Azar M.H. and Ahour F. Palladized Aluminum Electrode Covered by Prussian Blue Film as an Effective Transducer for Electrocatalytic Oxidation and Hydrodynamic Amperometry of N-acetyl-cysteine and Glutathione. J Electroanal Chem. 2008, 622(1), 22-28.
6. Barusa C., Grosa P., Comtata M., Daunes-Marionb S. and Tarrouxb R. Electrochemical Behavior of N-acetyl-L-cysteine on Gold Electrode: A Tentative Reaction Mechanism. Electrochim Acta. 2007, 52(28), 7978-7985.
7. Suarez W.T., Marcolino L.H. and Fatibello-Filho O. Voltammetric Determination of N-acetylcysteine using a Carbon Paste Electrode Modified with Copper (II) Hexacyanoferrate (III). Microchem J. 2006, 82(2), 163-167.
8. Helia H., Majdib S. and Sattarahmadyc N. Chemical: Ultrasensitive Sensing of N-Acetyl-L-Cysteine using An Electrocatalytic Transducer of Nanoparticles of Iron (iii) Oxide Core-Cobalt Hexacyanoferrate Shell.
Sens Actuators B. 2010, 145(1), 185-193.
9. Gao Z.N., Zhang J. and Liu W.Y., Electrocatalytic Oxidation of N-acetyl-L-cysteine by Acetylferrocene at Glassycarbon Electrode. J. Electroanal. Chem. 2005, 580(1), 9-16.
Table 2. Determination of NAC in spiked samples (n = 3).
Samples c (NAC)/mM RSD (%) Recovery (%)
Initial Added Found
1 10.0 10 20.94 4.2 104.7
2 10.0 15 25.77 3.9 103.1
3 10.0 20 28.41 4.6 94.7
10. Ojani R., Raoof J.B. and Zarei E. Electrocatalytic Reduction of Nitrite using Ferricyanide; Application for its Simple and Selective Determination. Electro-chim Acta. 2006, 52(3), 753-759.
11. Raoof J.B., Ojani R. and Rashid-Nadimi S. Preparation of Polypyrrole/Ferrocyanide Films Modified Carbon Paste Electrode and its Application on the Electrocatalytic Determination of Ascorbic Acid. Electrochim Acta. 2004, 49(2), 271-280.
12. Raoof J.B., Ojani R. and Rashid-Nadimi S. Voltam-metric Determination of Ascorbic Acid and Dopamine in the Same Sample at the Surface of a Carbon Paste Electrode Modified with Polypyrrole/Ferrocyanide Films. Electrochim Acta. 2005, 50(24), 4694-4698.
13. Dong W., Dong C., Shuang S. and Choi M.F. Near-infrared Luminescence Quenching Method for the Detection of Phenolic Compounds using N-acetyl-l-cysteine-protected Gold Nanoparticles-tyrosinase Hybrid Material. Biosens Bioelectron. 2010, 25(5), 1043-1048.
14. El-Hady D.A. Selective and Sensitive Hydroxypropyl-beta-cyclodextrin Based Sensor for Simple Monitoring of (+ )-catechin in Some Commercia1 Drinks and Biological Fluids. Anal Chim Acta. 2007, 593(2), 178-87.
15. Lee C.J. and Yang J. a-Cyclodextrin-modified Infrared Chemical Sensor for Selective Determination of Tyrosine in Biological Fluids. Anal Biochem. 2006, 359(1), 124-131.
16. Kang S., Cui Z. and Mu J. Electrochemical Behavior of Sodium Cholate and Deoxycholate on an Electrode Modified with Multi-walled Carbon Nanotubes (MWNTs) Linked Up with Cyclodextrin. Diamond RelatMater. 2007, 16(1), 12-15.
17. Wang G., Liu X., Yu B. and Luo G. Electrocatalytic Response of Norepinephrine at a b-cyclodextrin Incorporated Carbon Nanotube Modified Electrode. J
Electroanal Chem. 2004, 567(2), 227-231.
18. Shen Q. and Wang X. Simultaneous Determination of Adenine, Guanine and Thymine Based on P-cyclodextrin/MWNTs Modified Electrode. J
Electroanal Chem. 2009, 632(1-2), 149-153.
19. He J., Yang Y., Yang X., Liu Y., Liu Z., Shen G. and Yu R. P-Cyclodextrin Incorporated Carbon Nanotube-modified Electrode as an Electrochemical Sensor for Rutin Sens. Actuators B. 2006, 114(1), 94-100.
20. Zhao J., Jin J., Wu C., Jiang H., Zhou Y., Zuo J. and Wang X. Highly Sensitive Identification of Cancer Cells by Combining the New Tetrathiafulvalene Derivative with a b-cyclodextrin/Multi-walled Carbon Nanotubes Modified GCE. Analyst. 2010, 135(11), 2965-2969.
21. Karimi-Maleh H., Keyvanfard M., Alizad K., Fou-ladgar M., Beitollahi H., Mokhtari A., Gholami-Orimi F. Voltammetric Determination of N-actylcysteine using Modified Multiwall Carbon Nanotubes Paste Electrode. Int JElectrochem Sci. 2011, 6, 6141-6150.
22. Salmanipour A., Taher M.A., Beitollahi H., Hossein-zadeh R. New Voltammetric Strategy for Simultaneous Determination of N-acetylcysteine and Folic Acid using a Carbon Nanotube Modified Glassy Carbon Electrode. Colloids Surf B. 2013, 102, 385-390.
23. Ensafi A.A., Karimi-Maleh H., Mallakpour S., Hatami M. Simultaneous Determination of N-acetylcysteine and Acetaminophen by Voltammetric Method using N - (3,4-dihydroxyphenethyl) - 3,5 - dinitrobenzamide Modified Multiwall Carbon Nanotubes Paste Electrode. Sens Actuators B. 2011, 155(2), 464-472.
24. Keyvanfard M., Ensafi A.A., Karimi-Maleh H., Ali-zad K. Modified Multiwalled Carbon Nanotubes Paste Electrode as a Sensor for the Electrocatalytic Determination of N-acetylcysteine in the Presence of High Concentrations of Folic Acid. Anal Methods. 2012, 4(10), 3268-3274.
25. Song J.P., Guo Y.J., Shuang S.M. and Dong C. Electrochemical Behavior of Brilliant Cresyl Violet at Multi-wall Carbon Nanotubes/Nafion Modified Glassy Carbon Electrode and its Interaction with Cyclodextrins. J Incl Phenom. Macro. 2009, 64(1-2), 115-120.
26. Song J.P., Guo Y.J., Shuang S.M. and Dong C. Study on the Supramolecular Systems of Two Basic Violets with Cyclodextrins by MWNTs/Nafion Modified Glassy Carbon Electrode. Chin Chem Lett. 2009, 20(8), 981-984.
27. Davis J.J., Coles R.J. and Hill H.A.O. Protein Electrochemistry at Carbon Nanotube Electrodes. J
Electroanal Chem. 1997, 440(1-2), 279-282.
28. Wang Z.H., Wang Y.M. and Luo G.A. A Selective Voltammetric Method for Uric Acid Detection at Beta-cyclodextrin Modified Electrode Incorporating Carbon Nanotubes. Analyst. 2002, 127(10), 1353-1358.
29. Chen J., Dyer M.J. and Yu M.F. Cyclodextrin-Mediated Soft Cutting of Single-Walled Carbon Nanotubes. J Am Chem Soc. 2001, 123(25), 6201-6202.
30. Yan J.L., Sun R.D. and Sun W.D. Electrochemical Behavior of N-Acetyl-L-cysteine at Mercury Film Electrode. Chin J Anal Chem. 2003, 31(4), 448-450.
31. Gao Z.N., Zhang J. and Liu W.Y. Electrocatalytic Oxidation of N-acetyl-L-cysteine by Acetylferrocene at Glassy Carbon Electrode. J Electroanal Chem. 2005, 580(1), 9-16.
32. Bai L. and Gao Z.N. Electrocatalytic Oxidation of N-Acetyl-L-Cysteine at 10-Methylphenothiazine Modified Carbon Paste Electrode and Its Practical Analytical Application. Chin J Appl Chem. 2008, 25(6), 702-705.
33. Ren C.C. and Gao Z.N. Electrocatalytic Oxidation and its Electroanalytical Method of N-Acetyl-L-Cysteine at Multi-wall Carbon Nanotubes Modified Glassy Carbon Electrode. Chin J Pharm Anal. 2009, 29(7), 1183-1186.
34. Raoof J.B., Ojani R., Amiri-Aref M. and Chekin F. Catechol as an Electrochemical Indicator for Voltam-metric Determination of N-acetyl-L-cysteine in Aqueous Media at the Surface of Carbon Paste Electrode. J Appl Electrochem. 2010, 40(7), 1357-1363.
35. Carmo D.R., Silva R.M. and Stradiotto N.R. Electro-catalytic and Voltammetric Determination of Sulf-hydryl Compounds Through Iron Nitroprusside Modified Graphite Paste Electrode. JBraz Chem Soc. 2003, 14(4), 616-620.
Received 18.06.13 Accepted 05.11.14
Реферат
Электрохимическое поведение и электроанализ ^ацетил^-цистеина (NAC) были изучены на стеклянном углеродном электроде, модифицированном с использованием р-циклодекстрина (P-CD) и углеродных нанотрубок (Р-CD/MWCNT/GCE). р-CD/MWCNT/GCE показывает хорошую электрокаталитическую активность при реакции каталитического окисления NAC. Поскольку NAC селективно обогащен p-CD, р-CD/MWCNT/GCE может решить проблемы затухания сигнала, вызванные адсорбцией
NAC. Было также рассмотрено влияние экспериментальных условий на электрохимическое поведение NAC при использовании модифицированных электродов. Механизм и кинетика реакций каталитического окисления NAC контролировались с помощью циклической вольтамперометрии и хроноамперомет-рии. Константа скорости каталитического окисления £(4,21+0,05) х 103 М-1С-1 была рассчитана с использованием электрохимических методов. Результаты показали, что в 1,0х10-3 М растворе гексацианоферрата калия ток был пропорционален концентрации NAC от 4,4х10-4 М до 8,0х10-2 М [Ip (10-6 A) = 0,58412 + + 5,38х103 c(M), R2 = 0,9934], и пределом обнаружения (S/N = 3) было 5,02х10-5 М. Для 80 мМ NAC, шесть последовательных измерений показали относительное стандартное отклонение 3,4%, что говорит о том, что сенсор имеет хорошую точность измерения. Предложенный метод может применяться для определения NAC при каждом анализе.
Ключевые слова: химически модифицированный электрод, углеродные нанотрубки, циклодекстрин, N-ацетил-Ь-цистеина, электрохимическое определение.
