CC BY
46STH MULTIDISCIPLINARY INTERNATIONAL
Conference of Biological Psychiatry
«Stress and Behavior»
Proceedings of the 9th International Multidisciplinary Conference «Stress and behavior» Saint-Petersburg, Russia, 16-19 May 2005 Editor: Allan V. Kalueff, PhD
CONFERENCE ABSTRACTS 2. SLEEP AND BEHAVIOR
EFFECTS OF HEAT SHOCK PROTEIN 70 kDa AND QUERCETIN ON BLOOD PARAMETERS DURING A STATE OF REST AND ACTIVE WAKEFULNESS IN RATS
T.N. Mankovskaya, A.V. Novozhilov Sechenov Institute of Evolutionary Physiology and Biochemistry RAS, St. Petersburg, Russia;
Institute of Physiology NAS, Minsk, Belarus;
Ivanovo State University, Ivanovo, Russia
Heat shock proteins (HSP) play an important role in the living organisms. Under normal conditions, HSP70 are known to work in cells as the molecular chaperones folding and translocating cytoplasmic proteins. Members of HSP70 family are powerful cytoprotectors against damaging factors acting on a cell or an organism (Margulis, Guzhova, 2000). Now it is established that HSP70 possesses a somnogenic effect (Pastukhov et al., 2004). «Normal» sleep is a strong anti-stress factor, whereas its long-lasting disturbance may lead to various diseases. During the last years, the interest to biologically active polyphenols from family of flavonoids markedly increased. Most members of this family (including quercetin) are antioxidants (Kahraman et al., 2003). Quercetin seems to be used as an artificial trap of free radicals in vivo. At the same time, quercetin is well-known as a strong inhibitor of HSP70 expression (Pshennikova et al., 1998). Effect of HSP70 and quercetin on biochemical and hematological parameters of blood has not yet been investigated. In this study, the research objects were the whole blood and plasma of Wistar rats. Guide cannulaes were implicated in the III brain ventricle and in the jugular vein under Nembutal anesthesia. Each animal was placed in an individual cage and kept in a sound attenuated room at 20—22 °C, with food and water ad libitum. All animals preliminary were tested in the open field to select the most active rats. HSP70 purified from lipopolisaccharide (Institute of Cytology RAS; Guzhova et al., 1998), and quercetin (Sigma), were used in this study. The animals were injected with HSP70 (III ventricle of the brain, 1.5 mkg/1 mkl) or quercetin (i.p., 5 mg/kg). In the control group, saline was injected in the same volumes. In both control and experimental animals, staying in a state of rest and active wakefulness, the blood was taken from jugular vein: 1) 30—40 min before the injection; 2) 30—40 min and 3) 2.5—3 h after the injection. It was shown in a separate series that 10— 20% of the drowsiness and 80—90% of non-rapid-eye-movement sleep (NREMS) correspond to a state of rest. The standard biochemical sets were used to determine the content of glucose, cholesterol and triglyceride. Standard hematological parameters and acid erythrocyte resistance were identified in plasma. Our results showed that acid erythrocyte resistance in intact resting rats (with prevalence NREMS) is greater than in active awake animals. It may reflect an increase in emission of young forms of erythrocytes as well as a resistance to acid action during the NREMS. The data coincide with concepts that the NREMS is the powerful anti-stress factor. During the NREMS, muscular tone, corticosterone levels and vegetative functions decrease and the synthesis of growth hormone and anabolic processes increase. No changes in erythrocyte resistance in resting (vs. intact) animals after a microinjection of saline or HSP70 werefound. At the same time, an increase in erythrocyte resistance in active wakefulness after microinjections of HSP70 (vs. both intact and saline controls) was observed. Hence, an injection of HSP70 alters population compound of blood erythrocytes due to emission of more resistant young forms. We suggest that such effect of HSP70 may be mediated by activation of vegetative centers and neurosecretory systems of hypothalamus. In contrast to HSP70, quercetin
ISSN 16G6-S1S1
reduced acid resistance of erythrocytes since 3.5 h after the injection there was an acceleration of erythrocyte disintegration. This suggests that altered population of erythrocytes include higher number of old erythrocytes non-resistant to the acidic action. Quercetin inhibits HSP70 expression in various tissues. Our present data show that injections of HSP70 do not affect concentrations of glucose, cholesterol and triglyceride in plasma. Quercetin reduces cholesterol and does not affect glucose and triglyceride. The study was supported by RFRF grant 03-03-33034.
ISSN 16G6-S1S1
