CC BY
9'22
ALT'" B-P-5
BIOMEDICAL PHOTONICS
Effect of CaCO3 on Optical Parameters of Biological Tissues
in Normal and Cancer
E. N. Lazareva,12* D. K. Tuchina,1'2'3 A. A. Doronkina,1 R. A. Anisimov,1 M. V. Lomova,1 A. M. Mylnikov,4 N. A.
Navolokin,4'5'6 V. I. Kochubey,12 I. Yu. Yanina12
1- Saratov State University (National Research University), Institute of Physics, 83 Astrakhanskaya str., Saratov 410012, Russia 2- Tomsk State University (National Research University), Laboratory of laser molecular imaging and machine
learning, 36Lenin's av., Tomsk 634050, Russia 3- A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, 33Leninskyprospect, building 2Moscow, 119071, Russia 4- Saratov State Medical University, Department of Pathological Anatomy, 112 B Kazachaya str., Saratov 410012, Russia
5- Saratov State Medical University, Center for Collective Use of Experimental Oncology, Experimental Department,
112 B Kazachaya str., Saratov 410012, Russia
6- State Healthcare Institution «Saratov City Clinical Hospital No. 1 named after Yu.Ya. Gordeev», 19 Kholzunova st.,
Pathological Department, Saratov 410017, Russia *email address: lazarevaen@ list.ru
Currently, optical therapeutic and diagnostic methods are being actively developed, which are distinguished by their speed, low invasiveness and availability [1, 2]. Nanocontainers for targeted delivery help in the implementation of such methods [3]. In such cases, porous particles of calcium carbonate are used as carriers, which have a slight cytotoxic effect on living cells [4, 5]. For example, nanocontainers with dyes are used in photodynamic therapy to slow down the growth of tumor tissue [6, 7]. However, it is important to know the optical properties of tissues in the area of photodynamic exposure both for containers with dye and without, since this makes it possible to evaluate the effect of nanocontainers on the optical properties of tissues in the area of irradiation, which must be taken into account when calculating the loading of dye into containers.
This study shows the change in optical parameters, such as the absorption coefficient, scattering coefficient, anisot-ropy factor, of biological tissues before and after the introduction of calcium carbonate particles taken from the area of tumor development. The measurements were performed ex vivo at room and physiological temperatures, which makes it possible to approximate the obtained data to the actual conditions during photodynamic therapy.
The study was supported by RSFgrant no. 21-72-10057, https://rscf.ru/project/21-72-10057/.
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