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Ukrainian Journal of Ecology
Ukrainian Journal of Ecology, 2019,9(2), 151-155
ORIGINAL ARTICLE UDC: 006.015.4:637.54:636.087.7
Echinacea pallida extract effect on quils meat quality
R.A. Chudak, Y.M. Poberezhets, O.I. Vozniuk, V.O. Dobronetska
Vinnytsia National Agrarian University, 21008, 3, Soniachna Str., Vinnytsia, Ukraine
E-mail: romanchudak@ukr.net Received: 23.04.2019. Accepted: 24.05.2019
The usage of biologically active additives in animal diets provides maximum use of nutrients, it has a positive effect on digestion. Therefore, it contributes to the rational and economical use of feed, it increases its productivity, and livestock farming becomes economically feasible. It has been proved that using dry extract of Echinacea pallida for the feeding quails of meat breed Pharaoh helps to increase protein accumulation and reduce fat loss in the chest muscles.
The aim of the study was to research the physical and chemical parameters of the quails meat of the Pharaoh breed. To accomplish this goal an experiment was carried out on quails of the Pharaoh meat breed in accordance with generally accepted methods. Forming poultry groups, we took into account the live weight, age, sex, breed, productivity, conditions of maintenance and feeding. According to the research results, it was found that feeding quails by the extract of Echinacea pallida increases the percentage of dry matter in white meat of quails by 0.51% (P<0.001), the protein content is increased by 3.46% (P<0.001), the amount of fat is increased by 1.27% (P<0.001) compared with the control group. The use of phytobiotics for poultry feeding increases the level of dry matter in the femoral muscles by 0.25% (P<0.01), fat by 4.05%, and extractives without nitrogen by 1.72% (P<0.001) compared with benchmark. The additional consumption of the phytobiotic additive increases the content of phosphorus by 10.7% (P<0.001), calcium by 0.51 g per kg (P<0.001), manganese by 44.8% (P<0.01) and copper by 7.3 mg per kg (P<0.001) in the quails pectoral muscles compared with the first control group. The content of calcium increased by 22.4% (P<0.001), iron by 55.7% (P<0.001), zinc by 50.1% (P<0.001), manganese by 68.9% (P<0.001) and copper by 3.27 mg per kg (P<0.001) under the action of Echinacea pallida extract in the femur of the bird, relative to the control sample.
Keywords: Quails; feeding; mixed fodder; phytobiotics; meat quality; Echinacea pallida
Introduction
Recently scientists have found that numerous feed additives are used for feeding animals but they do not always have a positive effect on the quality of products. Meat products deterioration has been observed even the production technology has been carefully observed. Under current production conditions, this issue becomes important, it is connected with the introduction of advanced technologies for the new fodder products use, the use of products of chemical and microbiological synthesis in feeding animals (Brady, 2013; Ibatullin et al., 2017; Neporochna, 2016; Podolian, 2016; Shevchenko et al., 2017). Pollution-free and quality food products play an important role for the normal functioning of vital functions of the human body. Recently, foreign and domestic scientists have shown increased interest in the feed additives of natural origin research and use for animal feeding (Arczewska - Wlosek and Swiatkiewicz, 2012; Brady, 2013; Mnisi & Mlambo, 2017; Razanova, 2018; Podolian, 2017; Shevchenko et al., 2017; Santhi & Kalaikannan, 2017).
Phytogenous supplements (phytobiotics) are natural growth stimulants, they are of particular importance nowadays. They are derived from herbs, spices and plant extracts; they possess flavor and curative properties; they are used both in modern medicine and feeding farm animals (Hashemi and Davoodi, 2010; Hrodzynskyi, 1992; Podobed, 2007; Ragab & Ayat, 2011; Rahimi & Teymouri, 2011; Sahin & Tarkan, 2012).
Echinacea pallida is especially important among phytogenous supplements because it has the balanced content of biologically active substances, i.e., polysaccharides, nitrogen-containing substances, organic acids, glycosides, alkaloids, flavonoids, saponins, amara, essential oils, resins, tannins, minerals and phytomelanins. It has antioxidant, antimutagenic, antimicrobial, immunostimulant, radioprotective properties, it also increases the body's resistance, activates metabolism and improves the assimilation of nutrients and minerals feed (Gurbuz, Balev & Kurtoglu, 2010; Maass, 2013; Samorodov, Pospelov, 2013; Sahin & Tarkan, 2012; Tzu & Chungli, 2009).
The roots of Echinacea angustifolia and Echinacea pallida contain phytomelanin that has important pharmacological properties i.e., antioxidant, antiradical, antimutagenic, radioprotective and immunomodulatory. The amount of phytomelanin in the roots of Echinacea angustifolia and Echinacea pallida is 1.8% (Samorodov & Pospelov, 2013).
However, Echinacea pallida as a fodder additive for quails has not been researched yet; it causes the need for this research work.
Therefore, the research to determine the optimal dose of Echinacea pallida extract for the feeding of quails is relevant and has an important theoretical and practical value.
The aim of the study was to research the physical and chemical parameters of the quails meat of the Pharaoh breed.
Methods
To accomplish this goal an experiment was carried out on quails of the Pharaoh meat breed in accordance with generally accepted methods (Ibatullin & Zhukovsky, 2017; Kozyr & Svezhentsov, 2002). Forming poultry groups, we took into account the live weight, age, sex, breed, productivity, conditions of maintenance and feeding. We have used zootechnical, physiological, morphological, hematological, biochemical and statistical methods of research.
Fodder additive of Echinacea pallida dry extract is a brown powder of homogeneous composition with a characteristic smell and specific bitter taste. It is very soluble in water, slightly soluble in ethanol, and insoluble in etcitelene. This additive is gained from underground part of the plant, that is, its roots. The researched additive of Echinacea pallida dry extract was obtained on the basis of LLC Experimental Plant of State Scientific Center of Medicines in Kharkiv. The main biologically active substances of this additive are polysaccharides i.e., fructosans, phenolic compounds such as hydroxycinnamic acids that has anti-inflammatory, antimicrobial and adaptogenic actions (Samorodov & Pospelov, 2013). Fodder additive of Echinacea pallida dry extract was added to the basic diet of quails of meat breed Pharaoh in the experiment (Table 1).
Table 1. Experimental Scheme._
Number of Duration of the
Group animals in group, head experiment, days Feeding habit
1-control 50 56 BD (mixed fodder)
2-experimental 50 56 BD+Echinacea pallida (6 mg per kg of live weight)
3- experimental 50 56 BD+Echinacea pallida (12 mg per kg of live weight)
4- experimental 50 56 BD+Echinacea pallida (18 mg per kg of live weight)
*BD - basic diet
Two hundred one-day age quails of meat breed Pharaoh were selected for experiment. Four groups of poultry (1 control and 3 experimental) were formed by analogues principle; each group had 50 heads, a live weight of quails was 8.0-8.1 g. Duration of the experiment was 56 days. Thirty-day age quails were divided into females and males (25 females and 25 males). The first control group received the basic diet during the experiment, and quails of experimental groups were fed different doses of Fodder additive of Echinacea pallida dry extract in addition to the basic diet. Control slaughter of experimental animals was conducted at the end of research.
The chemical, mineral composition and physical and chemical properties of meat were investigated by selecting samples from the thoracic and femur parts of the carcass; they were prepared by separating the skin, fat and connective tissue and thoroughly crushed.
The findings of the studies have been processed biometrically (Plohinsky, 1969). The indicators of the probability criterion by Student-Fisher were used at three levels *P<0.05, **P<0.01, ***P<0.001.
Results and discussion
It is known that quail meat has dietary properties; it is characterized by a gentle consistency, juicy, aromatic, high flavoring qualities, and slight bitterness. It has more vitamins, essential amino acids, macro- and microelements than chicken. It also contains 22% of protein and 3% of fat.
According to the research results, it was found that feeding quails by the extract of Echinacea pallida had a positive impact on the quality of quail meat.
Table 2. Chemical composition of quails pectoral muscles, %, (M ± m, n=4) (in air-dry substance).
Item
Group
1-control
2-experimental
3-experimental 4-experimental
Dry matter
Protein
Fat
89.56 ± 0.01 63.97 ± 0.35 11.59 ± 0.01
87.95 ± 0.009*** 66.27 ± 0.06*** 9.71 ± 0.01***
90.07 ± 0.01*** 67.10 ± 0.12*** 12.86 ± 0.01***
86.67 ± 0.01*** 67.43 ± 0.05*** 9.65 ± 0.009***
153 Echinacea pallida extract effect on quils meat quality
Ash 5.26 ± 0.01 4.87 ± 0.01*** 4.74 ± 0.01*** 4.94 ± 0.01***
NFE 8.74 ± 0.35 7.10 ± 0.09** 5.41 ± 0.12*** 4.70 ± 0.05***
Thus, if the average dose (12 mg per kg of live weight) of feed supplement is fed the dry matter amount in white quail meat is increased by 0.51 % (P<0.001). If we use the minimum and maximum doses (6 to 18 mg/kg), this item decreases by 1.61% and 2.89%, respectively (P<0.001) than in the control group.
It is known that white meat is better digested in the human gastrointestinal tract because it contains less connective tissue and more protein than red one.
It was found that the protein content in quails pectoral muscles of all experimental groups increased by 2.3%, 3.13% and 3.46% (P<0.001) than the first group.
Fat of poultry meat contains a large number of triglycerides with unsaturated fatty acids. That's why it is more efficiently digested.
The fat amount in quails muscle tissue of the third experimental group increased by 1.27% (P<0.001). However, the poultry of the second and fourth groups have decreased this indicator by 1.88% and 1.94% respectively (P<0.001) than control group. It should be noted that the ash content in the 2nd, 3rd and 4th experimental groups decreased by 0.39%, 0.52% and 0.32% (P<0.001) respectively.
It should be mentioned that the various doses of the researched fodder additive reduces the NFE content by 1.64% (P<0.01), 3.33% and 4.04% (P<0.001), respectively in white quail meat. Chemical composition of quails femoral muscles is presented in Table 3.
Table 3. Chemical composition of quails femoral muscles, %, ^ ± m, n=4) (in air-dry substance)._
Item Group
1-control 2-experimental 3-experimental 4-experimental
Dry matter 91.54 ± 0.05 91.18 ± 0.02*** 91.79 ± 0.007** 91.77 ± 0.009**
Protein 62.02 ± 0.04 59.97 ± 0.01*** 60.25 ± 0.08*** 60.06 ± 0.06***
Fat 18.03 ± 0.01 18.24 ± 0.009*** 22.08 ± 0.01*** 20.00 ± 0.007***
Ash 4.49 ± 0.01 4.24 ± 0.01*** 3.99 ± 0.01*** 4.22 ± 0.01***
NFE 7.04 ± 0.05 8.76 ± 0.02*** 5.49 ± 0.09*** 7.53 ± 0.10**
The use of phytobiotics for poultry feeding increases the level of dry matter by 0.25% and 0.23% (P<0.01) in the third and fourth group, respectively; in the second group this indicator decreases by 0.36% (P<0.001) in comparison with the first control group.
In all experimental groups the level of protein deposition was significantly less than the control sample has, respectively by 2.05%, 1.77% and 1.96% (P<0.001). The ash content decrease in the red quail meat of the groups fed by different doses of the extract of Echinacea pallida by 0.25%, 0.5% and 0.27% (P<0.001) was also observed.
It should be mentioned the fat amount in the femur muscle predominates in the second group by 0.21%, in the third by 4.05%, and in the fourth by 1.97% in comparison with the first control group. The minimum (second group) and the maximum (third group) doses of the researched additive increases the part of nitrogen free substances by 1.72% (P<0.001) and 0.49% (P<0.01), respectively. The research results have shown that changes in the mineral composition of pectoral muscles occur when we use different doses of Echinacea pallida extract (Table 4).
Table 4. Mineral content of quails pectoral muscles (М ± m, n=4)._
Mineral _Group_
elements_1-control_2-experimental_3-experimental_4-experimental
Р, g/kg 10.3 ± 0.06 11.4 ± 0.02*** 10.4 ± 0.12 11.0 ± 0.12**
Са, g/kg 0.84 ± 0.009 0.55 ± 0.014*** 1.35 ± 0.007*** 0.69 ± 0.012***
Mg, g/kg 0.72 ± 0.007 0.73 ± 0.007 0.62 ± 0.009*** 0.66 ± 0.007***
Fe, mg/kg 103.5 ± 1.00 99.5 ± 0.83* 92.8 ± 0.32*** 94.2 ± 0.52***
Zn, mg/kg 101.5 ± 0.21 98.5 ± 0.49** 95.1 ± 0.76*** 94.0 ± 0.39***
Mn, mg/kg 9.6 ± 0.25 13.9 ± 0.73** 1.9 ± 0.40*** 0.8 ± 0.01***
Cu, mg/kg 3.7 ± 0.03 8.5 ± 0.27*** 11.0 ± 0.29*** 10.4 ± 0.07***
Thus, about 10% of the total phosphorus content in the body is deposited in the poultry muscles. Meat products contains from 170-200 mg of this element per 100 g of raw product. The researched additive increased the content of phosphorus by 10.7% (P<0.001) and 6.8% (P<0.01) in the quails white meat of the second and fourth groups. However, in the third the group it was at the same level with the control one.
It should be noted that poultry of the third group has an increase in calcium in the pectoral muscles by 0.51 g per kg (P<0.001). At the same time, the poultry of the second and fourth experimental groups has lower calcium content in the pectoral muscles than the control analogues, respectively by 0.29 g/kg and 0.15 g/kg (P<0.001). It is known that magnesium is calcium antagonist in many muscles processes. If the quails are fed by Echinacea pallida extract the content of magnesium decreased by 13.9%, in the third group and by 8.3% (P<0.001) in the fourth group in accordance with the control parameters.
Iron is an integral part of proteins among which the muscle tissue myoglobin is the most important because is 3 to 5% of the dry weight of the tissue. It also plays an important role in the processes of tissue respiration and nutrition contributing to the live weight increase and the preservation of young animals. It was investigated that the iron content in white meat decreased by 3.9% (P<0.05), 10.3% and 8.9% (P<0.001) in the birds of the second, third and fourth experimental groups compared with first group. Zinc has a wide range of physiological effects, i.e., it participates breathing, it is a catalyst in oxidation-reducing processes, it also increases the activity of vitamins and enhances phagocytosis.
Due to the effects of various doses of the researched additive zinc decreases in the pectoral muscles of quails of all experimental groups, respectively, by 2.9% (P<0.01), 6.3% and 7.4% (P< 0.001) in comparison with the control sample. Manganese facilitates the growth of young animals, affects the processes of hematopoiesis, tissue respiration and improves the hydrocarbon, protein and copper metabolism. It was found that the second sample had the highest amount of manganese in white meat, it was by 44.8% (P<0.01) more than the control one has. However, the third and fourth samples have reduced manganese proportion by 80.2% and 91.7% (P<0.001).
Copper engages in hemogenesis and promotes the formation of hemoglobin in the blood, as well as is necessary for the normal development of the skeleton and improving meat productivity. It should be mentioned that usage of Echinacea pallida extract facilitates the copper accumulation in white quail meat; it was 4.8 mg/kg, 7.3 mg/kg and 6.7 mg/kg (P<0.001), respectively, in comparison with the first control group.
The mineral content of quails femoral muscles also had certain features (Table 5). Thus, the phosphorus amount in red meat of poultry from third and fourth groups increases by 8.6% and 6.4% (P<0.001) respectively. However, the second group had a tendency to decrease this indicator by 2.1% compared to the control. It should be noted that the poultry of the third and fourth group had increase in calcium content by 22.4% (P<0.001) and by 6.9% (P<0.05). However, the poultry of the second group has this indicator lower by 8.6% (P<0.01) than the control group has.
Table 5. Mineral content of quails femoral muscles, (M ± m, n=4).
Mineral Group
elements 1-control 2-experimental 3-experimental 4-experimental
P, g/kg 9.3 ± 0.02 9.1 ± 0.22 10.1 ± 0.05*** 9.9 ± 0.05***
Ca, g/kg 0.58 ± 0.009 0.53 ± 0.009** 0.71 ± 0.01*** 0.62 ± 0.01*
Mg, g/kg Fe, mg/kg 0.60 ± 0.01 62.1 ± 0.41 0.55 ± 0.01* 63.1 ± 0.46 0.59 ± 0.009 96.7 ± 0.60*** 0.56 ± 0.009* 59.5 ± 0.57**
Zn, mg/kg 62.6 ± 0.24 61.5 ± 0.15** 94.0 ± 0.89*** 59.8 ± 0.19***
Mn, mg/kg 8.7 ± 0.39 13.2 ± 0.28*** 14.7 ± 0.09*** 14.3 ± 0.02***
Cu, mg/kg 0.85 ± 0.06 4.12 ± 0.24*** 1.94 ± 0.12*** 1.38 ± 0.24
The amount of magnesium in all experimental groups slightly decreased in comparison with the control parameters, i.e., by 8.3% (P<0.05) in the second group, by 1.7% in the third group, and by 6.7% (P<0.05) in the fourth group. The iron content of quails femoral muscles increases by 55.7% (P<0.001) in the third experimental group. However, this indicator decreases by 4.2% (P<0.01) in the fourth group.
The level of zinc significantly increased by 50.1% (P<0.001) in the third experimental group. However it was less than control by 1.7% (P<0.01) and 4.5% (P<0.001) in the second and fourth groups respectively.
It was found that the use of Echinacea pallida extract facilitates an increase of manganese percentage in the red meat of the second group quails by 51.7%, the third - by 68.9%, and the fourth by 64.3% (P<0.001) than the control sample. It should be noted that the consumption of minimum and average dose of additive by poultry increases the level of accumulation of copper in the femoral muscle, respectively, in the second group by 3.27 mg/kg and in the third group by 1.09 mg/kg (P<0.001 ) compared to control.
Therefore, different doses of Echinacea pallida extract as a part of poultry feeds increases productivity; it also improves the quality of the quails meat production of.
Conclusions
It was found that feeding quails by the extract of Echinacea pallida increases the percentage of dry matter in white meat of quails by 0.51% (P<0.001), the protein content is increased by 3.46% (P<0.001), the amount of fat is increased by 1.27% (P<0.001) compared with the control group.
The use of phytobiotics for poultry feeding increases the level of dry matter in the femoral muscles by 0.25% (P<0.01), fat by 4.05%, and extractives without nitrogen by 1.72% (P<0.001) compared with benchmark.
The additional consumption of the phytobiotic additive increases the content of phosphorus by 10.7% (P<0.001), calcium by 0.51 g per kg (P<0.001), manganese by 44.8% (P<0.01) and copper by 7.3 mg per kg (P<0.001) in the quails pectoral muscles compared with the first control group.
155 Echinacea pallida extract effect on quils meat quality
The content of calcium increased by 22.4% (P<0.001), iron by 55.7% (P<0.001), zinc by 50.1% (P<0.001), manganese by 68.9% (P<0.001) and copper by 3.27 mg per kg (P<0.001) under the action of Echinacea pallida extract in the femur of the bird, relative to the control sample.
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Citation: Chudak, R.A., Poberezhets, Y.M., Vozniuk, O.I., Dobronetska, V.O. (2019). Echinacea pallida extract effect on quils meat quality. Ukrainian Journal of Ecology, 9(2), 151-155.
I (")E^^MI This work Is licensed under a Creative Commons Attribution 4.0. License
