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Journal of Stress Physiology & Biochemistry, Vol. 6 No. 1 2010, pp. 4-12 ISSN 1997-0838 Original Text Copyright © 2010 by Ratheesh Chandra, Abdussalam, Nabeesa Salim and Puthur
ORIGINAL ARTICLE
Distribution of Bio-accumulated Cd and Cr in two Vigna species and the Associated Histological Variations
Ratheesh Chandra, P., Abdussalam A.K., Nabeesa Salim and Jos T. Puthur*
Division of Plant Physiology and Biochemistry, Department of Botany, University of Calicut, Kerala, PIN - 673635. India.
Phone: 0494-2401144*406,407, Fax: 0494-2400269 * [email protected]
Received December 16, 2009
In nutrient culture experiments, bioaccumulation and anatomical effects of cadmium (CdCU - 20^M) and chromium (K2&2O7 - 600 ^M) on the structure of root and stem was studied by histochemical and analytical methods in Vigna radiata and Vigna unguiculata. Each metal exerted specific influences on the anatomy of various tissues in root and stem. Histochemical localisation of cadmium and chromium was observed in the stained sections of root and stem. Atomic Absorption Spectrophotometric study revealed maximum accumulation of cadmium and chromium in the root tissue as compared to shoot with significant variation among the species. Abundant occurrences of densely stained deposits of chromium were seen in the root stelar region of V. unguiculata and to a lesser extend in V. radiata. Cadmium accumulation in V. radiata was comparatively more than that of V. unguiculata. The findings also revealed that the accumulation pattern of cadmium and chromium varies between species and hence is species specific.
key words: Vigna radiata /, Vigna unguiculata, / Cadmium /, Chromium / Bioaccumulation
Distribution of Bio-accumulated Cd and Cr. ORIGINAL ARTICLE
Distribution of Bio-accumulated Cd and Cr in two Vigna species and the Associated Histological Variations
Ratheesh Chandra, P., Abdussalam A.K., Nabeesa Salim and Jos T. Puthur*
Division of Plant Physiology and Biochemistry, Department of Botany, University of Calicut, Kerala, PIN - 673635. India.
Phone: 0494-2401144*406,407, Fax: 0494-2400269
Received December 16, 2009
In nutrient culture experiments, bioaccumulation and anatomical effects of cadmium (CdCU -20^M) and chromium (K2&2O7 - 600 ^M) on the structure of root and stem was studied by histochemical and analytical methods in Vigna radiata and Vigna unguiculata. Each metal exerted specific influences on the anatomy of various tissues in root and stem. Histochemical localisation of cadmium and chromium was observed in the stained sections of root and stem. Atomic Absorption Spectrophotometric study revealed maximum accumulation of cadmium and chromium in the root tissue as compared to shoot with significant variation among the species. Abundant occurrences of densely stained deposits of chromium were seen in the root stelar region of V. unguiculata and to a lesser extend in V. radiata. Cadmium accumulation in V. radiata was comparatively more than that of V. unguiculata. The findings also revealed that the accumulation pattern of cadmium and chromium varies between species and hence is species specific.
key words: Vigna radiata /, Vigna unguiculata, / Cadmium /, Chromium / Bioaccumulation
Heavy metals are one of the key factors that exert negative influences on man and environment, and their release to environment cause toxicity to plants that are continually exposed to potentially toxic heavy metals. Cadmium is a metal pollutant which enters the environment mainly through anthropogenic and industrial processes and also through fertilizers.
(Foy et al., 1978, Sanita di- Toppi and Gabbrielli, 1999, Cakmak et al., 2000). Although cadmium adversely affects plant growth, (Baryla et al. 2001, Sandalio et al. 2001) root growth is severely affected and results in faster reduction of root biomass compared to the shoot resulting in an increased
shoot/root biomass ratio (Jalil et al., 1994, Wojcik and Tukendorf, 1999). According to Stolt et al. (2006) the rate of absorption and translocation of cadmium varies from plant to plant and genetic variation exists in the accumulation rate of the cadmium in different parts of the plant. Ederly et al. (2004) suggested that in Phragmitis australis, cadmium was accumulated mainly in the vacuoles of parenchyma cells of roots.
Chromium toxicity in plants is observed at multiple levels such as reduced yield, inhibited growth of leaves and roots, inhibition of enzymatic activities and mutagenesis (Clijsters and Van Assche, 1985, Bishnoi et al., 1993, Shanker et al., 2005). Toxic effects of chromium have been reported in water relations of plants (Vazques et al., 1987), mineral metabolism, growth and development (Rout et al., 1997), seed germination (Peralta et al., 2001), enzymatic activities and translocation of sugars (Zeid, 2001). Bioaccumulation of chromium have been reported in cauliflower, beet root and barley (Lahouti and Peterson, 1979), mung bean (Samantary and Das, 1997) and Albizia amara (Shanker et al., 2005)
Although a number of studies have been documented on the toxic effects of cadmium and chromium, there are very meagre reports on the localisation of these metals in various plant tissues and the concerned anatomical variations, especially in cultivated plants. The present study is an attempt to locate the distribution of cadmium and chromium in the root and stem tissues of V. radiata and V. unguiculata and see the species variation with regard to the above phenomenon. Further anatomical variations as a result of the accumulation of these heavy metals are also studied.
MATERIALS AND METHODS
Commercially available seeds of Vigna radiata (L.) Wilczek and Vigna unguiculata (L.) Walp. were used for the study. Healthy seeds were selected and surface sterilized with Q.1 % (w/v) aqueous solution of mercuric chloride for one minute with constant shaking. After decanting the disinfectant, the seeds were then washed thoroughly in double distilled water. Twenty seeds in triplicates were sown in Petri-dishes lined with filter paper and kept for germination.
Modified Hoagland solution (Epstein, 1972) was used for culturing the seedlings. Concentrations of cadmium chloride and potassium dichromate exhibiting 5Q% growth inhibition were selected for the treatments. (CdCb- 2Q ^M, K2&2O7- 4QQ ^M and CdCl2- 2Q ^M, K2&2O7- 6QQ ^M for V. radiata and V. unguiculata respectively). The germinated seedlings on the petri-dish was further transplanted into polypropylene containers (5QQ cm3) containing Hoagland medium along with the respective heavy metals. During transplantation, seedlings were inserted gently through the holes (1 cm2) made by wire net placed on the container by taking care that only the radicle was immersed in the nutrient solution. The containers were kept at 25 ± 2 °C RH 78%± 2 in the net house condition. Seedlings cultured in nutrient solution in the same type of containers described above without any heavy metal served as the control.
Treated and control seedlings were collected after three days of treatment and sampling was continued at an interval of 3 days up to 12 days. For histochemical studies, uniformly cut pieces of root and stem were fixed in FAA, dehydrated through alcohol- TBA series and embedded in paraffin wax (Johansen, 194Q, Berlyn and Miksche, 1976). Using a Leica Rotary Microtome (Model- RM 2125RT) individual blocks were cut at Ш ^m thickness. Deparaffinised sections were stained with Delafield’s hematoxylin (Berlyn and Miksche, 1976) and photomicrographs were taken by using Nikon camera (Model- ECLIPSE E4QQ)
Cadmium and chromium content in the root and stem tissues were analyzed using Atomic Absorption
Spectrophotometer (PERKIN ELMER Model A, Analyst 300). Sample preparation was done according to the method of Allan (1969). The data were analysed statistically and test of significance was done.
RESULTS AND DISCUSSION Bioaccumulation
In general more accumulation of Cr was observed in V. unguiculata in comparison with V. radiata, where as higher Cd accumulation was observed in V. radiata as compared to V. unguiculata (Table 1). Both in V. radiata and V. unguiculata Cd and Cr accumulation were observed to be the maximum in root tissues compared to shoot tissues. Localization of the cadmium in the roots has been reported in Phragmites australis grown in hydroponic medium artificially contaminated with cadmium (Ederli et al., 2004). According to Cseh, (2002) considerable amount of cadmium accumulates mostly in the root cell walls and inhibits elongation of root growth. In a study conducted in rice, it has been observed that Cd is highly mobile and gets translocated even to seeds (Reid et al., 2003). Even though both species are exposed to the same concentration (20 ^M) of CdCl2, the significant differences in the pattern of cadmium accumulation between the species may be due to difference in the tolerance level of these plants towards Cd. According to Yoon et al. (2006) metal accumulation varies with plant species and they point out towards the genetic variation as important in this context. The genetic variation may be expressed as differences in morphological and physiological characteristics of genotypes (Hansson et al., 2005, Stolt et al., 2006). Ishikawa et al. (2006) suggested that cadmium accumulation pattern varies with plant species and comparative studies on Zea mays,
Brassica juncea, Oryza sativa and Beta vulgaris revealed that Zea mays displayed the lowest accumulation of cadmium.
Chromium accumulation is comparatively lower in V. radiata, therefore it is present in small quantities in the shoot whereas maximum accumulation is shown by V. unguiculata. Shanker et al. (2005) suggested that generally in plants chromium translocation from root to shoot is very slow. Pulford et al. (2001) in a study with temperate trees confirmed that chromium is poorly taken up into the aerial parts and is held predominantly in the roots. In both species of Vigna chromium accumulation was very high in the roots compared to the stem (Table 1).
An important reason for enhanced accumulation of chromium in the root may be due the presence of organic acids in the root exudates which form complexes with chromium, there by making them available for the uptake by root. Srivastava et al. (1999) suggested that in Lycopercicum esculentum carboxylic acid and amino acids present in the root are involved in the enhanced uptake of chromium in the roots.
Anatomical Changes
In general histological variations were more predominant in stem and root of V. radiata as compared to V. unguiculata, on treatment with Cd, whereas treatment of Cr brought about predominant histological variations in stem and root of both species of Vigna. Cd treatment brought about distortion of cortical cell shape in stem and root of V. radiata (Fig. 1B&E), whereas tissues of root and stem was unaffected in V. unguiculata (Fig. 2B&E). While Cd could disorganize the root epidermis of V. radiata, the stem epidermis remained intact (Fig. 1B&E). Cr treatment brought about distortion of epidermis, cortex and stele in stem of V. radiate (Fig. 1C), whereas the distortion was limited to the cortex and stele and was to a lesser extend in V. unguiculata (Fig. 2C).
Fig. 1. Histological variations in the stem and root of Vigna radiata seedlings treated with Cadmium (CdCb) and Chromium (K2&2O7). A, B & C represents Vigna radiata stem control, Cd and Cr treated respectively. D, E & F represents Vigna radiata root control, Cd and Cr treated respectively. ^ indicates the densely stained deposits of chromium in the root stelar region
Fig. 2. Histological variations in the stem and root of Vigna unguiculata seedlings treated with Cadmium (CdCl2) and Chromium (K2Cr2O7). A, B & C represents Vigna unguiculata stem control, Cd and Cr treated respectively. D, E & F represents Vigna unguiculata root control, Cd and Cr treated respectively. ^ indicates the densely stained deposits of chromium in the root stelar region
The histological changes in the root treated with Cr resulted in highly distorted piliferous layer and cortex in both species. Even though the stelar portion of root appeared normal in both species, some artefacts in the stele were noticed in V. radiata and also densely coloured deposits were observed towards phloem of V. unguiculata to a larger extend and to a lesser
Earlier it was shown that roots of wheat grown individually with Ni, Cd and Pb, the epidermal cells were disorganized while the cortical cells exhibited distortion of cell shape due to disintegration. These heavy metals were also found to cause breakdown of root vascular tissues (Setia and Bala, 1994). It has also been reported that Cr brings about increased relative proportion of pith and cortical tissue layers (Suseela et al., 2002).
This study reveals that significant variation exists between the two Vigna species studied, with regard to the distribution of bioaccumlated heavy metals (Cd and Cr) and the associated histological variations. It can be very well presumed that the variation in the distribution may either be due to the differences in the mode of absorption or due to the species specificity as far as the tolerance or sensitivity towards heavy metal toxicity is concerned.
extend in V. radiata (Fig. 1F & Fig. 2F). Thickened cell walls of vessels and pith of V. unguiculata was another notable variation (Fig. 2F). The distortion of cells of various tissues may be the result of interference with the cell division or with cell elongation.
Acknowledgements
We are indebted to The Cashew Export Promotion Council (CEPC), Government of India, for the AAS facilities extended.
REFERENCES
AllanJ.E. (1969) The Preparation of Agricultural Samples for Analysis by Atomic Absorption Spectrometry. Varian Techtron Bulletin,
New Zealand.
Baryla,A., Carrier,P., Frank,F., Coulomb,C., Sahut,C., Havaux,M. (2001) Leaf chlorosis in oil seed rape plants (Brassica rapans) grown on cadmium polluted soil: causes and consequences for photosynthesis and growth. Planta, 212, 696-709.
Table 1. Distribution of bioaccumlated Cadmium and Chromium in plants treated with CdCl2 and K2&2O7. The data are the average of recordings from three independent experiments, each with a minimum of three replicates (i.e. n=9). The data represent mean + standard error.
Treatment Vigna radiata Vigna unguiculata
Cadmium Root (|ug/g dry wt.) 351±23 98±6.4
Stem 26±1.2 7Q±4.6
Chromium Root 58Q±41 275Q±98
(^g/g dry wt.) Stem 2Q±1.1 68Q±35
11 Distribution of Bio-
Berlyn,G.P., Miksche,J.P. (1976) Botanical Microtechnique and Cytochemistry. The lowa State University Press, Ames lowa.
Bishnoi,N.R., Sheoran,I.S., Singh,R. (1993) Effects of chromium on photosynthesis, respiration and nitrogen fixation in Pea (Pisum sativum L.) seedlings. J. Plant. Physiol., 142, 25-30.
Cakmak,I., Welch,R.M., Erenoglu,B., Romheld,V., Norvell,W.A., Kochian,L.V. (2000) Influence of varied zinc supply on retranslocations of cadmium (109Cd) and rubidium (86Rb) applied on mature leaf of Durum Wheat seedlings. Plant Soil. 219, 279-284.
Clijsters,H., Van Assche,F. (1985) Inhibition of photosynthesis by heavy metals. Photosynth. Res. 7, 31-40.
Cseh, E. (2002) Metal permeability, transport and efflux in plants. In Prasad,M.N.V., Strzalka,K. (Ed.), Physiology and Biochemistry of Metal Toxicity and Tolerance in Plants. Kluwer Academic Publishers, Dordrecht. pp 1-36.
Ederli,L., Reale,L., Ferrauti,F., Pasqualini,S. (2004) Responses induced by high concentration of cadmium in Phragmites australis roots. Physiol. Plant., 121, 66-74.
Epstein,E. (1972) Mineral Nutrition of Plants: Principles and Perspectives. John Wiley and Sons, New York.
Foy,C.D., Chaney,R.L., White,M.C. (1978) The Physiology of metal toxicity in plants.
Annu. Rev. Plant Physiol., 29, 511-566.
Hansson,D., Stolt,J.P., Asp,H., Merker,A. (2005) Rye chromosome with Cd regulating genes in wheat. Proceedings 8th International Conference on the Biogeochemistry of
Trace Elements. ICOBTE, Adelaide, Australia. 358-359.
Ishikawa,S., Ae,N., Murakami,M., Wagatsuma,T. (2006) Is Brassica juncea a suitable plant for phytoremediation of cadmium in soils with moderately low cadmium contamination?-Possibility of using other plant species for Cd phytoextraction. Soil Sci. Plant Nutr., 52, 32-42.
Jalil,A., Selles,F., Clarke,J.M. (1994) Effect of cadmium on growth and uptake of cadmium and other elements by Durum Wheat. J. Plant Nutr., 17, 1839-1858.
Johansen,D.A. (1940) Plant Microtechnique. Mc Graw Hills, New York.
Lahouti,M., Peterson,P.J. (1979) Chromium accumulation and distribution in crop plants.
J. Sci. FoodAgric. 30, 136-142.
Peralta,J.R., Gardea Torresdey,J.L., Tiemann,K.J., Arteaga,S., Rascon,E. (2001) Uptake and effects of five heavy metals on seed germination and plant growth in alfalfa
(Medicago sativa L.). Bull. Environ. Contam. Toxicol., 66, 727-734.
Pulford,I.D. Watson,C., McGregor,S.D. (2001) Uptake of chromium by trees: Prospects for phytoremediation. Environ. Geochem. Health. 23, 307-311.
Reid,R.J., Dunbar,K.R., McLaughlin,M.J. (2003) Cadmium loading into potato tubers: the roles of the periderm, xylem and phloem.
Plant Cell Environ., 26, 201-206.
Rout,G.R., Samantaray,S., Das,P. (1997) Different chromium tolerance among eight mung bean cultivars grown in nutrient culture. J. Plant Nutr., 20, 341-347.
Samantary,S., Das,P. (1997) Accumulation and distribution of chromium, nickel and iron in the mung bean plant. Fresnius Envir. Bull. 6, 633-641.
Sandalio,L.M., Dalurzo,H.C., Geomez,M., Romero-Puertas,M.C., del Rio,L.A. (2001) Cadmium induces changes in growth and oxidative metabolism of pea plants. J. Exp. Bot., 52, 2115-2126.
Sanita-di-Toppi,L., Gabbrielli,R. (1999) Response to cadmium in higher plants. Environ. Exp. Bot, 41, 105-130.
Setia,R.C., Bala,R. (1994) Anatomical changes in root and stem of wheat (Triticum aestivum L.) in response to different heavy metals. Phytomorphol., 44, 95-104.
Shanker,A.K., Cervantes,C., Loza-Tavera,H., Avudainayagam,S. (2005) Chromium toxicity in plants. Environ. Intl. 31, 739-753.
Srivastava,S., Prakash,S., Srivastava,M.M. (1999) Chromium mobilization and plant availability-the impact of organic complexing ligands. Plant Soil. 212, 203208.
Stolt,P., Asp,H., Hultin,S. (2006) Genetic variation in wheat cadmium accumulation on soils with different cadmium concentrations. J. Agron. Crop Sci, 192, 201-208.
Suseela,M.R., Sinha,S., Singh,S., Saxena,R. (2002) Accumulation of chromium and scanning electron microscopic studies in Scirpus lacustris L. treated with metal and tannery effluent. Bull. Environ. Contam. Toxicol., 68, 540-548.
Vazques,M.D., Poschenrieder,C.H., Barcelo,J. (1987) Chromium (VI) induced structural changes in bush bean plants. Ann. Bot., 59, 427-438.
Wojcik,M., Tukendorf,A. (1999) Cadmium tolerance of maize, rye and wheat seedlings. Acta Phys. Plant., 21, 99-107.
YoonJ., Cao,X., Zhou,Q., Ma,L.Q. (2006) Accumulation of Pb, Cu, and Zn in native plants growing on a contaminated Florida site. Sci. Total Environ., 368, 456-464.
Zeid,I.M. (2001) Responses of Phaseolus vulgaris to chromium and cobalt treatments. Biol. Plant, 44, 111-115.
