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0Diagnostics of bacteria using Fabry-Perot interference in silicon nanostructures of various morphologies
M. Wang1*, K.A. Gonchar1, P.A. Rachishena1, D.A. Nazarovskaia1, P.A. Domnin2, I.I. Tsiniaikin1, S.A. Ermolaeva2, L.A. Osminkina1
1-Lomonosov Moscow State University, Faculty of Physics, Leninskie gory 1, 119991, Moscow, Russia 2- National Research Center for Epidemiology and Microbiology N. F. Gamaleya Gamaleyi St., 18, 123098,
Moscow, Russia
* van.m17@physics.msu. ru
The most common optical sensors are based on the effects of light interference in thin layers of silicon nanostructures, such as porous silicon (PS) [1] or silicon nanowires (SNF) [2].
In this work three different nanostructures were studied: (i) porous silicon film (e-pSi) obtained by electrochemical (EC) etching of crystalline silicon substrate; (ii) sacrificial-etched porous silicon film (se-pSi) obtained by double electrochemical (EC) etching of a crystalline silicon substrate with the first e-pSi layer dissolved in NaOH solution; (iii) porous silicon nanowire layer (pSiNWs) obtained by metal-assisted chemical etching (MACE) of crystalline silicon substrate.
SEM micrographs of the obtained silicon nanostructures with various morphologies after adsorption of E. coli. Bacteria are presented in Figure 1.
Fig. 1. SEM micrographs of silicon nanostructures with various morphologies after adsorption of E. coli.
IR reflectance spectra of the samples were measured without and with E. Coli at a concentration of 106 CFU/ml. Then, the change in the effective optical thickness of the nanostructures (EOT = 2xLxneJf) was determined using fast Fourier transform, and the adsorption efficiency of the bacteria was measured.
As a result, this work showed the possibility of diagnosing bacteria using Fabry-Perot interference in silicon nanostructures.
The study was supported by the Russian Science Foundation grant No. 22-72-10062, https://rscf.ru/project/22-72-10062/.
[1] A. Jane, R. Dronov, A. Hodges, N.H. Voelcker, Porous silicon biosensors on the advance, Trends in biotech., vol. 27(4), pp. 230-239, (2009).
[2] K.A. Gonchar, S.N. Agafilushkina, D.V. Moiseev, I.V. Bozhev, A.A. Manykin, E.A. Kropotkina, A.S. Gambaryan, L.A. Osminkina, H1N1 influenza virus interaction with a porous layer of silicon nanowires, Mater. Res. Express, vol. 7, pp. 035002, (2020).
