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6
Vestnik FEB RAS. 2018. № 6 Supplement
UDC 547.655.6; 615.45; 678.6 DOI: 10.25808/08697698.2018.202.6S.080
E.A. VASILEVA, N.P. MISHCHENKO, S. KIKIONIS, E. IOANNOU, V. ROUSSIS, S.A. FEDOREYEV
Development of new drug forms based on echinochrome a using electrospun micro/nanofibers
Key words: echinochrome A, electrospinning, micro/nanofibers, pharmaceutical polymers, drug formulations
The most well-known sea urchin pigment echinochrome A (Ech A) is the active substance in the antioxidant drug Histochrome® produced in Russia from the sand dollar Scaph-echinus mirabilis that is used in cardiology and ophthalmology. Histochrome is available only in ampoules in the form of echinochrome di- and trisodium salts for intravenous injections or infusions, since Ech A is insoluble in water.
Recently appeared a large number of publications revealing a wide range of new pharmacological activities of Ech A, for example gastroprotective [1], anti-diabetic [4], antiallergic [2], and mitochondria-protective properties against cardiotoxic drugs [3]. Therefore, development of new drug forms based on echinochrome with various components able to increase its solubility in water, to provide targeted and controlled release of the drug preserving or enhancing its pharmacological properties to expand the limits of drug's application is of great interest.
We obtained electrospun micro-/nanofibers from commercially used in pharmaceutical technology polymers (polyvinylpyrrolidone, polycaprolactone, cellulose acetate, hypromellose and polyethylene oxide) with Ech A incorporated and performed the in vitro dissolution tests at pH values 1.2 and 6.8. The most promising materials appeared to be polycaprolactone, hypromellose and polyethylene oxide since they provided a prolonged relesase of Ech A at pH 6.8.
REFERENCES:
1. Anufrieva, A.V.; Lebed'ko, O.A.; Berezina, G.P.; Kozlov, V.K. Effect of echinochrome A on biogenesis of free radicals in the gastric mucosa of adolescents with erosive gastroduodenitis. Far East Medical Journal 2012, 1, 78-81.
2. Itoh, T., Fujiwara, A., Ninomiya, M., Maeda, T., Ando, M., Tsukamasa, Y., & Koketsu, M. (2016). Inhibitory Effects of Echinochrome A, Isolated from Shell of the Sea Urchin Anthocidaris crassispina, on Antigen-Stimulated
* VASILEVA Elena Andreevna - Junior Researcher; MISHCHENKO Natalia Petrovna - PhD, Leading Researcher; FEDOREYEV Sergei Alexandrovich - DSc, Head of Laboratory (G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia); KIKIONIS Stefanos - PhD, IOANNOU Efstathia - Professor, ROUSSIS Vassilios - Professor (Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece). *E-mail: vasilieva_el_an@mail.ru
This study was supported by the grant of the Ministry of Education and Science of Russian Federation (project RFMEFI61317X0076).
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3. Jeong, S. H., Kim, H. K., Song, I. S., Lee, S. J., Ko, K. S., Rhee, B. D., et al. (2014a). Echinochrome A protects mitochondrial function in cardiomyocytes against cardiotoxic drugs. Marine drugs, 12, 2922-2936.
4. Mohamed, A. S., Soliman, A. M., & Marie, M. A. S. (2016). Mechanisms of echinochrome potency in modulating diabetic complications in liver. Life sciences, 151, 41-49.
