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3DETERMINING THE AMOUNT OF TRICHOGRAMMA GENERATIONS BEING MULTIPLIED IN THE BIOLABORATORY
1Davlatova Feruzahon Anvarovna, 2Arslanov Mahammatsoli Turg'unovich
1,2 Andijan Agricultural and Agrotechnological Institute https://doi.org/10.5281/zenodo.7671176
Abstract. In this article, experimental work was carried out to determine the application rate for the control ofgrain moth eggs andfor the control of eggs of the cotton bollworm and other insect pests, and it was found that the rate of 5 g. It was found that an additional 0.025, 0.044, 0.06 and 0.088 g of Trichogramma.
Keywords: eggs grain mole, laboratory, trichogram.
Trichogramma breeding has been going on for several decades in the countries of Central Asia. Of course, the purpose of carrying out these works is to reduce the use of chemical means in practice, i.e. in carrying out the work of combating agricultural equine pests.
Trichogramma is mainly used against the eggs of moths and butterflies found in cotton and other crops, and in turn is multiplied in the eggs of grain moth in special biolaboratories. Many authors have provided information on the rate of reproduction of this species. In this case, 1 gram of trichogram damages 5 grams of grain moth. To date, no specialist has raised doubts or objections to this information. In the biolaboratory, the trichogram is placed before fully hatched (70-75%) to damage the eggs of the grain moth. Because trichoramas that have not lost their vitality during the use of trichorama are reused. This work is basically using the trichogram to its full potential, although it takes a little time.
Taking into account the above-mentioned information, in 2022, we conducted experimental work in order to determine which of the trichograms in different weights actually affected grain moths and how the trichograms flew out of them and which of the obtained weights corresponded to our intended goal.
Experiments were carried out according to the methodical manual developed by Shshepetilnikova, Popov, Grinberg and other authors. 5 variants and 4 returns (1, 0.85, 0.8, 0.75 and 0.7 g.) were carried out in carrying out these works.
For this, the template is 1 g. in 70-75% of the hatched trichogram, in the rest of the variants fully hatched (100%), the hatched trichogram was used when infected with grain moth eggs. We know that 1 g. There are 50,000 eggs in a grain moth egg. Taking into account that 1 female trichogram has an average fertility of 60, from which generation of them is 1 g. The possibility of using That is, it is envisaged how much the trichogram of each generation used here will change.
In the first experiment, using the trichogram from the new generation, and the rest of the experiment using the 2-4 generation trichogram, 100 harmful grain moths were first counted with a trichogram, and how many of them were female and male trichogram, and based on the obtained result, the trichogram weight was filled in according to the weight of each generation (1- table).
According to the results of the experiment, the following can be said. In this table, when 1st-generation trichograms were used for grain moth damage in practice, out of 100 trichograms taken for analysis, on average, 97.6 trichograms were found, and 60 were female and 37.6 were
male, and the gender ratio was 1:1.6. It turned out to be close to the recommended norm. In the use of the remaining generations, it was found that the amount decreased over time, in the 2nd and 4th generations, it gradually decreased from 95.6 to 91.0 units, and the ratio of female and male genes decreased from 1:1:3 to 1:1.
According to the results of the conducted experiment, the amount of Trichogramma offspring, which are reproduced during the season, has led to a gradual decrease.
Table 1
Determining their quality indicator for the practical use of trichogramma generations. A scientific educational biolaboratory belonging to AKSHATI. 2022
Versions The number of eggs obtained, pcs Flying trichograms Proporti ons Additionally, Compared to the 1st generation Female tr-mma
All ? 6
1-generation 100 97,6 60,0 37,6 1:1,6 - 25
2- generation 100 95,6 54,3 41,6 1:1,3 0,3 59
3- generation 100 94,0 50,0 44,0 1:1,13 0,46 100
4- generation 100 91,2 45,6 45,6 1:1,0 0,6 144
In laboratory conditions, when the generations were used alternately, it was observed that their quantity decreased over time and the ratio of sexes also changed.
In addition, it is necessary to fill the trichogram with the amount of them distributed against night eggs. In this case, it is necessary to extract trichograms from the first infected grain moth and take into account their quantity (Table 2).
Table 2 shows the number of trichograms and the ratio of sexes to fill 1 gram of trichogram. Analytical work was carried out on the results of the first table.
In order to bring one gram of trichogram to the norm, it was determined that additional trichogram was used in the first generation at the weight of 0.025; 0.044; 0.06 and 0.088 grams.
Table 2
Weighting trichograms by generations Scientific Central Laboratory of AQXAI (2022)
Versions The number of eggs obtaine d, pes Flying trichograms Additionall y> Compared to the 1st generation gender Weight of trichigramm a
% pes ? <? ?
1- generatio n 70000 97, 5 6825 0 42042 2620 8 1750 109 4 656 0,025
2- generatio n 70000 95, 6 6692 0 37876,7 2 2904 3 3080 174 6 133 4 0,044
3- generatio n 70000 94, 0 6580 0 34939,8 3086 0 4200 222 6 197 4 0,06
4- generatio n 70000 91, 2 6384 0 31920 3192 0 6160 308 0 308 0 0,088
This corresponds to the required level of biological efficiency when trichogram is used in practice.
According to the results of the conducted experiment, it is appropriate to pay attention to their quantity and gender ratio when using trichogramma offspring during the season.
REFERENCES
1. Arslanov M.T., Asanov K.A., Rashidov M.I. and the head. Methodical manual for breeding grain moth, Tashkent. 2000.37. p.
2. Metodicheskie ukazaniya po massovomu razvedeniyu i primeneniyu trichogrammy dlya borby s vreditelyami selskohozyyastvennyx kultur. shchepetilnikovoy V.A. Gusev G.V., Tron N.M. Moscow, "Colossus", Tashkent, 1978.
