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Mirkhamidova Panda, Doctor of biology, professor, E-mail: parida.mirxamidova@mail.ru Khujanazarov Uktam Eshtemirovich, Candidate of biology, associated professor, E-mail: khuzhanazarov74@mail.ru Babakhanova Dilnoza Bahodirovna,
Teacher,
E-mail: dilnoza.boboxonova@mail.ru Ismoilova Komila, Student of the 4th course, Mukhamedov Gofurjon Isroilovich, Doctor of chemistry, professor, E-mail: rector@tvchdpi.uz
DETERMINATION OF THE TOTAL AMOUNT OF ANTIOXIDANTS IN SOME MEDICINAL PLANTS IN KASHKADARYA RIVER BASIN
Abstract: In the scientific work, the total amount of antioxidants in the leafs of some medicinal plants growing in Kashkadarya river basin like as in Mentha, Melissa, Trichodesma, Peganium, Origanium, Rheum, Ziziphora, Alhagi, Rumex were defined. On the research general antioxidants are found in Mentha, Melissa, Trichodesma, Peganum, Origanum, Rheum, Ziziphora, Alhagi, Rumex, but the highest amount of the antioxidants was studied in Mentha, Melissa, Origanium.
Keywords: antioxidants, free radicals, Mentha, Melissa, Trichodesma, Peganium, Origanium, Rheum, Ziziphora, Alhagi, Rumex, triton X-100, 96% of alcohol, 0-fenontroline, extract.
Antioxidants are active combinations; these are sig- Some of them are used as a drip, a liquid extract for nificant for the organisms of plants and animals. And the the treatment of diseases [2; 4]. Natural antioxidants antioxidants are important to complete the task of natu- protect the organism from free radicals and increase the ral detoxication systems to take out the free radicals and tolerances to various adverse effects and slow down the radical forms from organisms [1]. contamination process. Natural antioxidants can be used Natural antioxidants include ascorbic acids, lemon to treat many diseases. In the absence of antioxidants in acids, polyphenols, flavonoids, carotinoids, cysteine, our organism a number of diseases can be aroused [1]. phospholipids, tocopheroles, vitamins A and K. Antioxidant peculiarities in some medicinal plants such It is known that fruit vegetables are rich to antioxi- as Inula helenium, Glycyrrhiza glabra, Calendula offici-dants and it is beneficial to eat regularly. Antioxidants nalis, Hypericum scabrum, Matricaria recutita and Ruare found in the following quantities: black cherries, all mex thyrsiflorus were studied [7]. kinds of beans, walnuts, almond, forest coconut, pista- Determination of the total amount of antioxi-chio, garlic onion and others [2]. dants in medicinal plants is important as a biologiAs well as in various fruit trees a large amount of vi- cal value. The main purpose of our research is to de-tamin C, which is also found to be antioxidant in the termine the total antioxidant quantity in the leaves of leaves of walnuts, peach, mulberry, cherry and vine medicinal plants such as Mentha, Melissa, Trichodes-trees were defined, its amount depends on the vegeta- ma, Peganum, Origanum, Rheum, Ziziphora, Alhagi, tion period of plans. Rumex thyrsiflorus in Upper parts of Kashkadarya river
DETERMINATION OF THE TOTAL AMOUNT OF ANTIOXIDANTS IN SOME MEDICINAL PLANTS IN KASHKADARYA RIVER BASIN
basin. Research was carried out in the various vegetative periods of the medicinal plants.
In determination of the total antioxidants in the leaves of medicinal plants, a solution of 1% of triton-X-100 in 96% alcohol was used. The method is based on oxidation of antioxidants by iron III - chloride. It is
Table 1. - Amount of
returned from Fe+3 to Fe+2 chloride, its quantity is determined by intensity of the color generated by the addition of 0-fenontrolin.
The resulted color complex was measured at 505 nm wave length in the spectrophotometer - 46. The results are shown in (table 1).
general antioxidants
№ The name of plants Spring Summer Autumn
1. Mentha asiatica 3900 ± 200 10500±150 5850±150
2. Melissa officinalis 11700±150 2400±150 1800±20
3. Trichodesma incanum 6300±19 4500±150
4. Peganum harmala 7060 ± 450 1800±150 3000 ± 300
5. Origanum tyttanthum 1575±150 10200±450 3450 ±150
6. Rheum turkestanicum 8400 ± 0 1350±150
7. Ziziphora pedicellata 8850±195 4200±150
8. Alhagi pseudalhagi 4200 ± 300 1537.5 ± 112.5 3600 ± 300
9. Rumex thyrsiflorus 8100±300 7200 ± 200 1950±150
Our results show that the highest amount of general antioxidants is in Melissa leaves in spring, it is 11700 mkg/g, and such high concentration was found in Mentha and Origanium. It is 1500 mkg/g in Mentha, 10200 mkg/g in Origanum. This indicator is determined at the time of blossoming.
The medicinal Mentha extract contains 3900 mkg/g in spring, 10500 mkg/g in summer, 5850 mkg/g in autumn. There are ether oil, carotene, ges-peridin, urosolat, olenat acids in the leaves of medicinal Mentha. Flavonoids have been identified in the leaves.
Mentha preparations are used in medicine for stomach - intestinal pains, abdominal diseases, cholecystits, gallbladder disease and hepatitis. The highest concentration of antioxidants in leaves of Melissa plants is observed in spring, it involves 11700 mkg/g, 2400 mkg/g in summer and 1800 mkg/g in autumn. In traditional medicine, these plants are used for strengthening the nervous system and boosting heart disease, as well as for treatment some diseases.
The highest amount of general antioxidants in Origanum is detected during its blossoming period and its amount gets 10200 mkg/g, 1575 mkg/g in the spring and
33450 mkg/g in the autumn. It was found that the highest concentration of the Origanium is in the spring [5].
According to the facts, the Origanium plant contain 0,6% of ether oils, 1,35% of kumanrin, 11,06% of polyphenols, 3,2% of flavonoids [4; 6]. Medicinal forms of the origanium plant influence as an effect of the appetite, improving of stomach-intestinal tract and pain relief.
Rumex blooms in May-June. Its fruits are ripen in July, grows as a weed, its root is used as raw material, and it contains anthracic syrups, flavonoids, ether oil, resin (pitch), vitamin K, mucous matters and other compounds [1]. In traditional medicine is used as drip, boil, extracts [2; 4].
According to results, it is shown that the highest of general antioxidant was determined in bloomed period of Rumex, it consists of 8100 mkg/g. It was observed that its concentration decreased. It consisted of 7200mkg/g in summer and 1950mkg/g in autumn.
The research results show that general antioxidants are available in the leaves of medical plants like Mentha, Melissa, Trichodesma, Peganum, Origanum, Rheum, Ziziphora, Alhagi and Rumex. Its highest amount is defined in Melissa, Mentha and Origanum.
References:
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Agadjanyan A.Kh., Agadjanyan A. A. Antioxidant peculiarities of some medicinal plants. Journal of biology, Armenia, - 2012. - 4 (64). - P. 76-79. 2. Ibragimov A.Ya. Medicinal plants. - Tashkent, - 2016. - 404 p.
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6. Mukhtorov M. 1000 cure to 1000 disease. - Karshi, - 2009. - 271 p.
7. Rogojin V. V. Practicum on biological chemistry. - Sankt-Petersburg-Moscow-Krasnodar, - 2006. - 225 p.
