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Iskandarova Shokhista Fekhruzovna, doctor of pharmacy, docent at the Faculty Training Qualification of Pharmacists Tashkent Pharmaceutical Institute Abdukhalilova Nilufar Sobirovna, assistant of the department "Technology of medicinal forms" Tashkent Pharmaceutical Institute
DETERMINATION OF THE AMOUNT OF CURCUMIN IN DRY EXTRACT OF TURMERIC (CURCUMA LONGA L.)
Abstract: This article presents the results of the development of the technology for obtaining the dry extract from Curcuma Longa L. A stepwise process for obtaining the dry extract using the technological scheme is described. Keywords: Turmeric (Curcuma Longa L.), technology, dry extract, technological scheme.
Turmeric (Curcuma Longa L.) is a perennial herb of the ginger family. One of the main active components of Curcuma longa L. is curcumin - polyphenol, the main representative of the group of curcuminoids. Present the antitumor, antioxidant and anti-inflammatory activity of curcumin has been confirmed. The components of turmeric were allocated carbohydrates (4.7-8.2%), essential oils (2.44%), fatty acids (1.7-3.3%), curcuminoids (curcumin, demetoxicurcumin and bisdemetoxicurcumin), the content which is approximately 2%, although it can reach 2.5-5.0% of the dry weight, as well as other polypeptides, such as turmeric (0.1% dry extract).
Turmeric is a plant antimicrobial substance; it simultaneously improves digestion and facilitates normalizing intestinal flora. Owing to this fact, Turmeric serves as a good antimicrobial substance for weakened and chronic patients. This medicinal plant has an important prophylactic significance in the countries of tropical Asia, preventing spreading multiple intestinal infections.
Turmeric rhizomes are used for medical and food purposes. In order to speed up the ripening of spices, the collected roots are scalded with boiling water before drying, destroying the cells with the tincture curcumin. Curcumin, has essential oil cells, in the process of further fermentation soaked the entire root. At the same time, the starch contained in the root is partially hydrolyzed, and partly forms a colloid.
Immunomodulatory properties of curcumin have been established. Under the influence of this substance, the total number of leukocytes and antibody-forming cells increases, and the phagocytic activity of macrophages increases. In animal tests, curcumin has been shown to increase the body's antitumor activity.
Curcumin is an antagonist of Barbamil and chlorproma-
zine.
Based on the foregoing, the purpose of our research was to determine the quantitative content of curcuminoids in the dry extract obtained on this medicinal plant.
An analytical sample of the raw material was grounded to a particle size passing through a sieve with openings of 1 mm. About 1 g of (so-called) crushed raw materials were placed in a flask with a thin section with a capacity of 100 ml, 50 ml of 95% ethyl alcohol were added, added to the reflux condenser and heated in a boiling water bath for 30 minutes. Hot extraction sieved through cotton in a volumetric flask for the extraction. The extraction was repeated once more with 50 ml of 95% ethyl alcohol under the conditions described, filtering the extraction into the same volumetric flask. After cooling, the extraction volume was adjusted to the mark with 95% ethyl alcohol. 20 ml of the solution was chromatographed on a liquid chromatograph at least 3 times. After analyzing the extraction from the raw material, the column was washed with 20 ml of 100% acetonitrile. Then for 20 minutes was balanced by the mobile phase. After that, under the same conditions, a solution of a reliably known sample of curcuminoid (curcumin, dismetoxicurcumin, bis-dismetoxicurcumin) was chromatographed. Then, the areas of each peak were determined. The results obtained for each injection were averaged.
The total content of curcuminoids was calculated by algebraic addition of the obtained results for three individual substances or in terms of the total area of 3 peaks per curcumin, proposed as CO.
As a result, it was possible to choose the optimal conditions for chromatographic separation of the main curcuminoids: Column Phenomenex Luna 5 ^m C18(2)100 A, 250x x 3 mm, system: acetonitrile - 0.03 M phosphate buffer with pH 3.0 1: 1, flow rate 0.5 ml / min; detection at 425 nm (maximum absorption of all dominant curcuminoids). The figures below show the research results:
DETERMINATION OF THE AMOUNT OF CURCUMIN IN DRY EXTRACT OF TURMERIC (CURCUMA LONGA L.)
Figure 1.
Figure 3.
Conclusions: It was established that in the obtained dry extract, based on a long Turmeric, the content of curcumin was 9.8%.
References:
1. Abu Ali ibn Sino Canon of Medical Science III - Tashkent, 1996.
2. Petrov V. I., Spasov A. A. Russian encyclopedia of dietary supplements.- M.: Geotar-Media, 2007.
3. Halmatov Kh. H., Kharlamov I. A., Mavlankulova Z. I. Medicinal plants of Central Asia.- T.: Abu Ali Ibn Sino, 1998.-P. 228-229.
