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Abstracts. PHYTOPHARM 2017
and nutraceutical industries because it is one of best source of bioactive components including, triterpinoids, polysaccharides, benzenoids, benzoquinone derivatives, and maleic/succinic acid derivatives. Due to the rareness and cost effect, scientist developed several
culture techniques for the mass production. Recent pre-clinical and clinical studies on human strongly suggest that Antrodia cinnamomea could be novel alternative phytotherapeutic agent, or a synergizer in the treatment of cancer and other immune-related diseases.
DETECTION OF COUMARIN BY HPLC ANALYSIS IN CINNAMON SPICES FROM TURKISH MARKETS
© N. Yagmur Kumser Diker1, Gurbet Ünal2, Zehra Büyüktuncer Demirel2, I. Irem Tatli Qankaya
1 Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Botany, Ankara, Turkey;
2 Hacettepe University, Faculty of Health Sciences, Department of Nutrition and Dietetics, Ankara, Turkey
Cinnamon is generally used in many desserts to regulate insulin and blood glucose in Turkey. However, it can contain coumarin and the coumarin is a hepatotoxic natural compound found in different Cinnamomum species, specially in Cinnamomum cassia (L.) J. Presl. In this study, 22 spice samples and known cinnamon species [Cinnamomum cassia(L.) J. Presl and Cinnamomum verum J. Presl.] were performed using HPLC-DAD method. Each ground cinnamon sample (0.5 g) from different brands of the markets was extracted with 25 mL methanol (80%) by stirring with a magnetic stirrer at room temperature for 30 min. Samples were filtered and evaporated to dryness. Remain extract was adjusted to 5 mL in volumetric flask after dissolved in distillated water, then, the samples were lyophilized. Stock solutions of coumarin and samples (1 mg/mL) were prepared in methanol (50%). Although, the analysis method developed by Ding, Y. et al. was directly used for fingerprint analysis [1], for the detection of coumarin, a modified HPLC separation [2] was carried out using a reversed phase (RP-18, 5 ^m, 250 mm x 4.6 mm i.d.) column. The mobile phase made up of water: 5 mM ammonium acetate buffer, (0.2% (v/v) acetic acid)
(A) and acetonitrile/methanol 1:2 (v/v) (B) in a gradient elution: 0-2 min: 70% A; 2-30 min: 70-20% A; 30-35 min: 20-0% A; 35-45 min: 0% A; 45-55 min: 70% A. The sample injection volume was 5 and the flow rate was
0.8.mL/min. The analysis was performed at a wavelength of 279 nm. Even though, coumarin was not detected in standards and 4 samples, it was shown that 18 samples contained coumarin in the range between 0.3 and 4.9 mg in 1 g powder cinnamon. It is suggested that spice products should be evaluated for their quality according to tolerable daily intake (TDI) of 0.1 mg coumarin/kg body weight that limited by European Food Safety Authority (EFSA) [3]. Additionally, in fingerprint analysis, 2-methoxy cinnamaldehyde and eugenol peaks that were shown in known spices were observed in only one sample among 22 samples. This was also thought to depend on the quality of the product.
References:
1. Ding Y et al., 2011. Food Chemistry, 127:755-760.
2. Sproll C et al., 2008. Food Chemistry, 109:462-496.
3. EFSA. 2004. EFSA Journal, 104:1-36.
CHEMICAL COMPOSITION, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF THE ESSENTIAL OILS (EOS) OF DIFFERENT PINUS SPECIES FROM KOSOVO
© Fatbardhe Kurti1,2, Giangiacomo Beretta2, Behxhet Mustafa13, Fabrizio Gelmini2, Avni Hajdari13
1 Department of Biology. Faculty of Mathematical and Natural Science. University of Prishtina, Prishtine, Kosovo;
2 Department of Pharmaceutical Sciences, Universita degli Studi di Milano, Milan, Italy;
3 Institute of Biological and Environmental Research. University of Prishtina, Prishtine. Kosovo
Chemical profile, antioxidant and antimicrobial activity of total and fractionated EOs (F1 - hexane, F2 -hexane/diethyl ether, F3 - diethyl ether) derived from five Pinus species (Pinus heldreichii, P. peuce, P. mugo, Pinus nigra, P. sylvestris), were investigated. The hydrodistilled
EOs and their chromatographic fractions (direct solid phase extraction, SPE) were analysed by GC-MS and 112 compounds separated and identified. The main constituents were a-pinene, p-pinene, D-limonene, p-caryophyllene, germacrene D, bornyl acetate and
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] vol. 15/2017/suppLement 1
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