CC BY
3DOI 10.24412/CL-37135-2023-1-8-8
DEEP PENETRATION SUPERRESOLUTION IMAGING WITH ADAPTIVE OPTICS AND MULTI-FOCAL STRUCTURED ILLUMINATION
CHENSHUANG ZHANG, YONG ZHANG, DANYING LIN, BIN YU, JUNLE QU
Center for Biomedical Optics and Photonics, College of Physics and Optoelectric Engineering,
Shenzhen University
ABSTRACT
Optical imaging is crucial for biomedical research and clinical diagnosis. Improved depth and resolution are necessary for studying synaptic functions, neuronal structures, and microvessels in living animals' brain tissue. We developed an adaptive optics-based MSIM system (AO-MSIM) to overcome the limitations of multi-focus structured illumination microscopy (MSIM) when imaging thick samples. By generating a multi-focus excitation lattice using a spatial light modulator, we achieved multi-focus structured illumination scanning imaging. Aberrations in the excitation and emission paths were corrected using wavefront information, a spatial light modulator, and a deformable mirror. With pixel relocation and deconvolution image reconstruction algorithms, AO-MSIM achieved 150nm spatial resolution at a 500^m imaging depth, demonstrating its application in imaging thick brain tissue samples.
