CC BY
10
Vestnik FEB RAS. 2018. № 6 Supplement
UDC 547.99+615.277.3
DOI: 10.25808/08697698.2018.202.6S.056
E.S. MENCHINSKAYA , S.A. DYSHLOVOY, E.V. IVANETS, E.A. YURCHENKO
Cytotoxicity of some marine fungi metabolites against cancer cells
Key words: cancer cells, cytotoxicity, marine fungi metabolites
The aim of this study was investigate the cytotoxic activity of some low-molecular secondary metabolites of marine fungi-micromycetes. The influence of 38 compounds on the viability of mice neuroblastoma cells Neuro 2a, as well as human breast cancer cells MCF-7 and prostate cancer 22Rv1, was studied by MTT test.
It was found that neoehinulines B and C from the Vietnamese fungi Eurotium niveoglaucum demonstrated a cytotoxic activity against mice Neuro2a cells. Their EC50 concentrations were 50.9 ^M and 40.6 ^M, respectively. 4''-Dehydroxycandidusine A and candidusine A were less toxic EC50 = 78.9 ^M and 75.7 ^M, respectively.
N-methylpretrichodermamide B 1 at a concentration of 100 ^M induced the death of 47% of the neuroblastoma cells. It was found earlier, that N-methylpretrichodermamide B was highly cytotoxic against 22Rv1, PC-3, and LNCaP cancer cells with IC50 0.51, 5.11, and 1.76 ^M, respectively.
The highest cytotoxic activity was demonstrated by sesquiterpenoid nitrobenzoyl esters 9a,14-dihydroxy-6p-p-nitrobenzoylcinnamolide 2 with EC50 = 4.9 ^M, while its analogue 3 did not affect to viability of cells. Treatment human breast cancer cells (MCF-7) with compound 2 shown a less cytotoxic effect (EC50 = 59.6 ^M), compared to mice neuroblastoma cells, whereas compound 3 is practically inactive. The effect of compound 1on drug-resistant prostate cancer cells 22Rv1 was very significant with EC50 =3^M. Compound 2 was no toxic against this cancer cells also.
1 2 3
Thus, the same marine fungal metabolites have big potential as anticancer compounds.
O
* MENCHINSKAYA Ekaterina Sergeevna- PhD, Researcher, DYSHLOVOY Sergey Anatolyevich- PhD, Researcher, IVANETS Elena Vladimirovna - Graduate Student, YURCHENKO Ekaterina Aleksandrovna - PhD, Researcher (G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia). *E-mail: dminae@mail.ru
The work was supported by RFBR grant № 18-34-00737
