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Mirkosimova M.S.
2nd year master's student of Tashkent Pharmaceutical Institute
CREATION OF A NEW TYPE OF OILS USED TO TREAT VITILIGO
Annotation: Vitiligo is one of the current topics in medicine today. The origin of this disease and the lack of a clear treatment method cause many problems in the treatment process. Not all medications used to treat this disease work well. Our main goal is to create an effective drug from the extract of medicinal plant products used in the treatment of vitiligo. We hope that the drug prepared from the extract and excipients obtained will definitely give the results we expected.
Keywords: Psoralen, extraction, lanolin, ultraviolet light, viscosity, pharmaceutical psoralen, vitamins, trace elements.
Relevance of the topic: Vitiligo (Latin vitiligo - a disease of the skin) - the loss of normal pigment in some parts of the skin. The causes of vitiligo, popularly known as leprosy, are not yet fully understood in advanced medicine. That is why it is difficult to treat. This disease does not choose age, sex and race. 1-4% of the world's population suffers from vitiligo. However, there has been a recent increase in the number of cases among young people.
Many of the medications developed to date cause skin allergies, and oral medications may be less effective than ointments. The development of drugs with low side effects and high efficacy is one of the most important and urgent issues facing modern pharmaceuticals.
Purpose of the topic: The main purpose of our topic is to isolate the active substance from plant products, which is effective in vitiligo, by analyzing it, adding vitamins, trace elements and excipients, and preparing a type of ointment from it.
Experimental part: Psoralen production technology: The raw material for the production of psoralen was Psoralen drupaceous Beg, a fruit of the legume family Fabaceae.
Extraction of Coumarins from the Raw Material: The crushed white fruits were placed in a stainless-steel mixing extractor with a rotation speed of 60 rpm and a certain amount was extracted using 40% ethyl alcohol. Extraction was performed at room temperature. Over time, the alcoholic extract was filtered through an inert gas into a drunk filter and dropped into a collector. The water-alcohol extract took it to the next level. The short was washed with water and the water-alcohol mixture was regenerated.
Technical psoralen extraction: The collection of alcoholic extracts was evaporated using hot steam in a vacuum evaporator at a pressure of 100-150 mm Hg. The residual cube was placed in a glass reaction boiler and left at room
temperature for 1 day. The psoralen precipitated on the bottom of the pot. The liquid was decontaminated and the sediment was removed. Some of the sediment was washed with water, compacted, and air-dried.
Pharmaceutical psoralen extraction: Dried technical furocoumarins were poured into a mortar and then thoroughly mixed with Al2O3 in 2 parts. The index was elated using benzene.
The benzene eluate was filtered using a Büchner funnel with a paper filter and evaporated at 50-600 C in a rotary apparatus at a column pressure of 50-100 mm Hg. The psoralen precipitated. The sediment was filtered off.
In the first stage, a qualitative analysis of the substances contained in it was carried out by taking a portion of the extract obtained.
The following qualitative reactions were performed to determine the presence of coumarins.
1. Lactone reaction. Take two test tubes, add 2 ml of alcohol extract, add 0.5 ml of 10% sodium hydroxide solution to one of the test tubes, heat both tubes in a water bath until boiling and then cool. Both tubes were filled with 4 ml of distilled water. The solution in a solution of alkali and water formed a clear yellow color. Hence, this reaction confirms that it contains a lactone group. A few drops of dilute hydrochloric acid were added to this solution, the clear yellow solution lost its color and formed a precipitate or turbidity.
The amount of coumarins in the extract was determined by spectrophotometric and photo colorimetric methods.
In the next step, we got a type of ointment by mixing psoralen and lanolin and adding to it the vitamins and micronutrients needed for the pigment layer of the skin.
Preparation: Take 0.1 g of powdered psoralen, put it in a container with 5 g of anhydrous lanolin and mix well. Add 5 g of Vaseline oil and mix. Vitamins of group B and C from vitamins, microelements Cu, Zn, Fe from microelements were added to the resulting grease and mixed until a homogeneous mass was formed.
The parameters of the new ointment were analyzed.
Results obtained: In determining the authenticity, the isolated substance gave a micro sublimation reaction.
The new ointment is white in color, has a specific odor, and when the quality indicators were studied, it was determined that it actually contains coumarin by specific reactions. size 65 am, pHi-7.5, the content of heavy metals was found to be 0.0018%. These results were assessed as satisfactory.
Methods and techniques used: Extraction methods were used to extract biologically active substances from raw materials. Photocolorimetry, spectrophotometric methods were used to determine the amount of furocoumarins. Chromatography, distraction, lactone reaction, micro sublimation was used to determine the authenticity. An isometric method was used to
determine the viscosity. The appearance and odor of the ointment were studied by organoleptic methods.
Experimental and comparative methods were widely used in the experiment.
Conclusion: In our research on the development of a new form of ointment used in the treatment of vitiligo, we have studied the causes of the disease and its treatment, the literature on the drugs used, and identified the main active ingredient in the selected herbal product. we checked its content using special methods. The addition of excipients to the extracted main active biologically active substance and its enrichment with vitamins and microelements showed satisfactory results in the study of the quality indicators of the new ointment.
Reference:
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