CC BY
11B-I-19
Correlations between the blood microrheologic and microcirculation parameters in cardiological patients as determined by laser-optic methods
Y. Gurfnkel1, A. Priezzhev2, A. Lugovtsov2, P. Ermolinskiy2, L. Dyachuk3
1Lomonosov Moscow State University, Medical Research and Educational Center, MOSCOW,
Russian Federation
2Lomonosov Moscow State University, Department of Physics and International Laser Center, Moscow, Russian Federation
3Lomonosov Moscow State University, Medical Research and Education Center, Moscow, Russian Federation
The results of studying the hemorheological properties of the blood from scientific laboratories increasingly translated to cardiology clinics in order to implement them in treating those patients, in particular, who use antiplatelet agents and anticoagulants to improve blood viscosity and reduce the risk of blood clots in the heart cavities.
Recently we compared the blood aggregation parameters (aggregation index, characteristic time of aggregate formation, aggregation force and time) measured in vitro by laser aggregometry (LA) and optical trapping (OT) techniques with the parameters of blood rheology measured in vivo using vital digital capillaroscopy (VDC) in the nail bed capillaries. Overall 88 patients suffering from the arterial hypertension (AH) and patients with coronary heart disease (CHD) were enrolled in the study.
Our study revealed statistically significant correlation between the numbers of red blood cells (RBC) aggregated in the whole blood sample during the first 10 seconds of spontaneous aggregation in vitro and the presence of aggregates in the blood capillaries in vivo. Existence of correlation was also shown between the decrease in the average time of RBC aggregation measured in vitro and the decrease in the capillary blood-flow velocity (CBV) measured in vivo. The most pronounced deterioration in the rheological properties of blood in vivo and in vitro were found for patients with CHD. Measurements conducted with OT on single RBC in suspension showed statistically significant differences in the aggregation time for patients with AH and CHD (p < 0.05) depending on the CBV obtained by the VDC. The aggregation time measured on single RBC with OT depending on and presence or absence of stasis in blood flow detected by VDC revealed no significant differences for the group of hypertensive patients (p > 0.05), whereas for the group of patients with CHD, statistically significant differences were obtained (p < 0.05).
Our results demonstrate the possibility of using laser aggregometry and optical trapping for estimating the alterations of microrheologic and, consequently, microcirculation parameters. Alterations of RBC aggregation parameters measured in vitro can be used to evaluate the alterations of vital capillary blood flow parameters in human body. The earliest detection of microcirculation disorders is the best way to prevent possible complications of cardiovascular diseases and thus provide a well-timed start of treatment.
The Russian Science Foundation supported this study with the grant No. 18-15-00422.
