CC BY
0Correlation of pathologic alterations of microrheologic and microcirculation parameters measured by laser-optical
techniques
A.V. Priezzhev*, A.E. Lugovtsov, P.E. Ermolinskiy, D.A. Umerenkov, Yu.I. Gurfinkel
Lomonosov Moscow State University, 1-2 Leninskiye Gory, Moscow, Russia, 119991
* avp@biomedphotonics.ru
The goal of this work was to assess the correlation of pathologic alterations of red blood cells (RBC) deformability, i.e. the dependance of their elongation on shear stress, and aggregation properties, i.e. hydrodynamic strength of RBC aggregates, characteristic time of RBC aggregates formation, aggregation index, forces of pair aggregation of RBC, aggregation rate and degree of platelets, measured in vitro in blood samples drawn from patients suffering from socially important diseases and from healthy donors (control group) with alterations of the microcirculation parameters measured in these individuals in vivo. In vitro measurements were performed using the techniques of diffuse light scattering, turbidimetry, laser diffractometry, laser trapping and manipulation [1]. Digital capillaroscopy was used in vivo to visualize the capillaries and quantitatively evaluate the capillary blood flow in the nailfold vessels [2].
It was shown that in the groups of patients suffering from type 2 diabetes mellitus (DM) and cardiovascular diseases (CVD), the ability of RBC to deform is slightly reduced while the aggregation rate and forces of the cells interaction are significantly increased relative to those in the control group [3]. The degree and rate of platelets aggregation in patients suffering from DM and CVD are increased compared to the control group. The blood microcirculation in nailfold capillaries is impaired as well. The calculated correlation matrices have shown that the alterations of the parameters measured in vivo and in vitro for patients with different stages of these diseases are interrelated [4]. Good agreement between the results obtained with different techniques, and their applicability for the diagnostics of abnormalities of rheological properties of blood were demonstrated.
This work was supported by the Russian Science Foundation (Grant No. 23-45-00027).
[1] A. Lugovtsov, Y. Gurfinkel, P. Ermolinskiy, A. Maslyanitsina, L. Dyachuk, A. Priezzhev, Optical assessment of alterations of microrheologic and microcirculation parameters in cardiovascular diseases, Biomedical Optics Express, vol. 10, pp. 3974-3986 (2019).
[2] P. Ermolinskiy, Yu. Gurfinkel, E. Sovetnikov, A. Lugovtsov, A. Priezzhev, Correlation between the capillary blood flow characteristics and endothelium function in healthy volunteers and patients suffering from coronary heart disease and atrial fibrillation: A pilot study, Life. 13(10) (2043).
[3] A. Maslianitsyna, P. Ermolinskiy, A. Lugovtsov, A. Pigurenko, M. Sasonko, Yu. Gurfinkel, A. Priezzhev, Multimodal diagnostics of microrheologic alterations in blood of coronary heart disease and diabetic patients, Diagnostics 11, 76 (2021).
[4] I. Kadanova, A. Neznanov, A. Lugovtsov, Yu. Gurfinkel, A. Pigurkenko, L. Dyachuk, A. Priezzhev. Relationship between capillary blood flow parameters measured in vivo and blood microrheological parameters measured in vitro in arterial hypertension and coronary heart disease, Regional Blood Circulation and Microcirculation, 20(1), pp. 17-24 (2021). (in Russian).
