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BIOMEDICAL PHOTONICS
Complex of NaYF4:Yb,Er -HSA-MB for cancerous tumour destruction
E. N. Lazareva,12 A. A. Doronkina,1 D. K. Tuchina,1'2'3 A. M. Mylnikov,4 N. A. Navolokin,4'5'6
V. I. Kochubey,12 I. Yu. Yanina12
1- Saratov State University (National Research University), Institute of Physics, 83 Astrakhanskaya str., Saratov 410012, Russia 2- Tomsk State University (National Research University), Laboratory of laser molecular imaging and machine
learning, 36 Lenin's av., Tomsk 634050, Russia 3- A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, 33Leninsky prospect, building 2 Moscow, 119071, Russia 4- Saratov State Medical University, Department of Pathological Anatomy, 112 B Kazachaya str., Saratov 410012, Russia
5- Saratov State Medical University, Center for Collective Use of Experimental Oncology, Experimental Department,
112 B Kazachaya str., Saratov 410012, Russia
6- State Healthcare Institution «Saratov City Clinical Hospital No. 1 named after Yu.Ya. Gordeev», 19 Kholzunova st.,
Pathological Department, Saratov 410017, Russia e-mail: [email protected]
Upconversion nanoparticles (UCNPs) are of interest as novel luminescent probes for numerous applications in bio-photonics. In this paper, we present data of the stable surface coating of the UCNPs with human serum albumin (HSA) and methylene blue (MB).
One of the promising materials for the development of biophotonics therapy methods are functionalized UCNPs, which can not only increase the effectiveness of therapy, but also expand the diagnostic tool. Cross-linking of a photo-sensitizer (PS) with a shell ofNPs requires an additional coating of their surface. HSA nanoshells are biocompatible with many cell types over a wide range of concentrations, [1] are easily internalized by cells, [1, 2] are non-immunogenic, and have a long half-life in the circulation [3, 4].
Some PSs, such as MB, spontaneously bind to albumin, which makes it quite easy to cover the surface of NPs with a dye. The choice was based on the overlap of the NP luminescence and PS absorption bands.
Spectral measurements of the optical characteristics of biological tissues were carried out after intravenous administration of the complex of NaYF4:Yb,Er -HSA-MB to laboratory animals. For histological studies, samples of skin, organs and cancer were taken by surgery from rats.
The effect of intravenous injection of the complex of NaYF4:Yb,Er -HSA-MB on the change in the optical characteristics of biological tissues was shown in comparison with the injection of UCNPs.
The results of histological analysis of biopsy of organs and tumors of laboratory animals showed weak complex toxicity. The changes in the liver and lungs were reversible. Myocardial edema developed in the heart. A suggested way of excreting complex of NaYF4:Yb,Er -HSA-MB and the body is through the urine. The accumulation of complex occurs in the spleen, which is consistent with the data of other researchers. We have proposed a complex promising for photodynamic therapy.
The study was supported by a grant Russian Science Foundation No. 21-72-10057, https://rscf.ru/project/21-72-10057/
[1] K. Michaelis, M. M. Hoffmann, S. Dreis, E. Herbert, R. N. Alyautdin, M. Michaelis, J. Kreuter, K. Langer, Covalent linkage of apolipoprotein e to albumin nanoparticles strongly enhances drug transport into the brain, J. Pharmacol. Exp. Ther., vol. 317, pp. 1246-1253 (2006).
[2] J. M. Irache, M. Merodio, A. Arnedo, M. A. Camapanero, M. Mirshahi, S. Espuelas, Albumin nanoparticles for the intravitreal delivery of anticytomegaloviral drugs, Mini Rev. Med. Chem., vol. 5, pp.293-305 (2005).
[3] A. Loureiro, N. G. Azoia, A. C. Gomes, A. Cavaco-Paulo, Albumin-Based Nanodevices as Drug Carriers, Curr. Pharm. Des., vol. 22, pp. 1371-1390 (2016).
[4] F. F. An, X. H. Zhang, Strategies for Preparing Albumin-based Nanoparticles for Multifunctional Bioimaging and Drug Delivery,
Theranostics, vol. 7, pp. 3667-3689 (2017).
