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Abstracts
COMPLEX METHOD FOR THE STUDY OF VOLUME-DEPENDENT RED BLOOD CELL BEHAVIOR
Parshina, E.Yu., Yusipovich, A.I., Maksimov, G.V., and Rubin, A.B.
Biological Department, M.V. Lomonosov Moscow State University, Moscow, Russia
The investigations of processes following cell volume changes require a complex of methods to reveal and visualize structural alteration of cell membrane and cytoplasm. In our lab we developed combination of approaches that allows us to investigate red blood cells under change of media osmolality, as a rule accompanied by changes of cell volume [1-3].
Laser interference microscopy (LIM) technique makes possible to investigate cytoplasmic alteration in living cells. LIM method in combination with a direct method for cell volume measurement may be employed for estimation of morphological changes and for assessment of redistribution of cell substances in the cytoplasm evoked by diverse stimuli including osmotic stress.
Micro Raman spectroscopy allows estimating conformation of hemoglobin in erythrocyte and atomic force microscopy (AFM) gives precise information about cell geometric dimensions and physical properties of cell surface. We have showed that the combination of these methods provide detailed information about the distribution of cytosolic hemoglobin inside RBC.
Red blood cell surface relief studied by AFM demonstrated volume-dependent alterations under change of media osmolarity. Results propose an implication of a two-dimensional membrane carcass in volume sensing and/or signal transduction.
References
1. Yusipovich, A.I., Parshina, E.Yu, Brysgalova, N.Yu, Brazhe, A.R., Brazhe, N.A., Lomakin, A.G., Levin, G.G., Maksimov, G.V. Laser interference microscopy in erythrocyte study. Journal of Applied Physics, 2009, vol. 105, p. 102037.
2. Yusipovich, A.I., Zagubizhenko, M.V., Levin, G.G., Platonova, A., Parshina, E.Y., Grygorzcyk, R., Maksimov, G.V., Rubin, A.B., Orlov, S.N. Laser interference microscopy of amphibian erythrocytes: impact of cell volume and refractive index. Journal of Microscopy, 2011, vol. 244, no. 3, p. 223-229.
3. Parshina, E.Yu, Yusipovich, A.I., Platonova, A.A., Grygorczyk, R., Maksimov, G.V., Orlov, S.N. Thermal in-activation of volume-sensitive K+,Cl- cotransport and plasma membrane relief changes in human erythrocytes. Pflugers Archiv European Journal of Physiology, 2013, p. 1-7 DOI.
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Бюллетень сибирской медицины, 2013, том 12, № 4, с. 24-68
