CC BY
156
Journal of Stress Physiology & Biochemistry, Vol. 8 No. 3 2012, pp. 214-223 ISSN 1997-0838 Original Text Copyright © 2012 by Nasibi, Fazelian and Rezazadeh
ORIGINAL ARTICLE
Comparison the effects of nitric oxide and spermidin pretreatment on alleviation of salt stress in chamomile plant (Matricaria recutita L.)
Fazelian Nasrin 1, Fatemeh Nasibi*2, Ramezan Rezazadeh 3
1 Shahid Bahonar University of Kerman, Iran
2 Biology Department, Shahid Bahonar University of Kerman, Iran
3 Plant and Seed Improvement Department, Hormozgan Agricultural Research Center, Bandar Abbass, Iran
Tel: 0983413222032, Fax: 0983413222032
* E-mail: [email protected]
Received June 9 2012
Salt stress is an important environmental stress that produces reactive oxygen species in plants and causes oxidative injuries. In this investigation, salt stress reduced the shoot and root length, while increased the content of malondealdehyde, Hydrogen peroxide, and the activity of Ascorbate peroxidase andguaiacol peroxidase. Pretreatment of chamomile plants under salt stress with sodium nitroprussideand Spermidin caused enhancement of growth parameters and reduction of malondealdehyde and Hydrogen peroxide content. Pretreatment of plants with sodium nitroprusside remarkably increased Ascorbate peroxidase activity, while Spermidin pre-treatment significantly increased guaiacol peroxidase activity. Application of sodium nitroprusside or Spermidin with Methylene blue which is known to block cyclic guanosine monophosphate signaling pathway, reduced the protective effects of sodium nitroprussideand Spermidin in plants under salinity condition. The result of this study indicated that Methylene blue could partially and entirely abolish the protective effect of Nitric oxide on some physiological parameter. Methylene blue also has could reduce the alleviation effect of Spermidin on some of parameters in chamomile plant under salt stress, so with comparing the results of this study it seems that Spermidin probably acts through Nitric oxide pathway, but the use of2-4- carboxyphenyl- 4,4,5,5- tetramethyl-imidazoline-1-oxyl-3-oxide is better to prove.
Key words: Antioxidant enzymes /Methylene blue /Polyamines/Salinity /Sodium nitroprusside
ORIGINAL ARTICLE
Comparison the effects of nitric oxide and spermidin pretreatment on alleviation of salt stress in chamomile plant (Matricaria recutita L.)
Fazelian Nasrin 1, Fatemeh Nasibi*2, Ramezan Rezazadeh 3
1 Shahid Bahonar University of Kerman, Iran
2 Biology Department, Shahid Bahonar University of Kerman, Iran
3 Plant and Seed Improvement Department, Hormozgan Agricultural Research Center, Bandar Abbass, Iran
Tel: 0983413222032, Fax: 0983413222032
* E-mail: [email protected]
Received June 9 2012
Salt stress is an important environmental stress that produces reactive oxygen species in plants and causes oxidative injuries. In this investigation, salt stress reduced the shoot and root length, while increased the content of malondealdehyde, Hydrogen peroxide, and the activity of Ascorbate peroxidase andguaiacol peroxidase. Pretreatment of chamomile plants under salt stress with sodium nitroprussideand Spermidin caused enhancement of growth parameters and reduction of malondealdehyde and Hydrogen peroxide content. Pretreatment of plants with sodium nitroprusside remarkably increased Ascorbate peroxidase activity, while Spermidin pre-treatment significantly increased guaiacol peroxidase activity. Application of sodium nitroprusside or Spermidin with Methylene blue which is known to block cyclic guanosine monophosphate signaling pathway, reduced the protective effects of sodium nitroprussideand Spermidin in plants under salinity condition. The result of this study indicated that Methylene blue could partially and entirely abolish the protective effect of Nitric oxide on some physiological parameter. Methylene blue also has could reduce the alleviation effect of Spermidin on some of parameters in chamomile plant under salt stress, so with comparing the results of this study it seems that Spermidin probably acts through Nitric oxide pathway, but the use of2-4- carboxyphenyl- 4,4,5,5- tetramethyl-imidazoline-1-oxyl-3-oxide is better to prove.
Key words: Antioxidant enzymes/Methylene blue /Polyamines /Salinity /Sodium nitroprusside
Excess amount of salt in the soil adversely affects plant growth and development. Processes such as seed germination, seedling growth, vegetative growth and flowering are adversely
affected by high salt concentration, ultimately causing reduced economic yield and a quality of produce. High salt concentrations decrease the osmotic potential of soil solution creating a water
stress in plants. Secondly, they cause severe ion toxicity, since Na+ is not readily sequestered into vacuoles. Finally, the interactions of salts with mineral nutrition may result in nutrient imbalances and deficiencies. The consequence of all these can ultimately lead to plant death because of growth arrest and molecular damage. Different plant species have developed different mechanisms to cope with these effects in response to salt stress (McCue and Hanson 1992). However, exposure of plants to salt stress can increase the production of reactive oxygen species (ROS). These species are highly reactive and can damage chlorophyll, proteins, lipids and nucleic acids (Foyer and Noctor, 2000). Plants process both enzymatic and non-enzymatic mechanisms are designated to minimize the concentration of ROS.
Nitricoxide (NO) is as mall and lipophilic gas and a bioactive molecule that play an important role in different physiological processes. There is increasing evidence showing that NO acts like a signal molecule in processes such as growth and development, respiratory metabolism, cell death, and ion leakage (Kopyra and Gwozdz 2004; Lamotto et al., 2005). On the other hand, NO can also mediate plant growth regulators and ROS metabolism and increasingly evident have shown, which is involved in signal transduction and responses to biotic and abiotic stress such as drought, low and high temperatures, UV and ozone exposure, heavy metal, herbicides, cold, and salt stress (Neill and Desikan 2003; Del Rio et al., 2004; Fan et al., 2007).Tolerance to drought, salt and heat stress was enhanced in wheat (Triticum aestivum) and rice (Oryza sativa) seedlings when the plants were treated with NO donor, sodium nitroprusside (Mata and Lamattina 2001; Uchida et al., 2002). In plant cells, the diamineputrescine (Put),
triaminespermidine (Spd) and tetraminespermine (Spm) constitute the major PAs. They are known to be essential for growth and development (Tabor and Tabor 1984). The protective role of PA in plant stress reported in many studies (Zhao and Yang, 2008; Zhao et al., 2008, Liu et al., 2006).In previous researches it has been reported that the many effects of polyamines in alleviation of stresses may be related to production of NO and NO signaling pathway (Tun et al., 2006). It is an interesting study of using Methylene blue (MB) to inhibit the NO signaling pathway. In organism, NO may act through activation of guanylatecyclase, which produces the second messenger cyclic GMP (cGMP), or through s-nitrosylation of redox-sensitive transcription factors or ion channels (Stamler, 1994). It has been reported that (MB) inhibits soluble guanylatecyclase and thereby the action of NO and cGMP (Keaney et al., 1994; Paciullo et al., 2010). There is another NO inhibitors or scavengers such as (2-4- carboxyphenyl- 4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (PTIO) but study on MB is lesser than PTIO. Based on the above observations, the objective of the present experiment was comparing the physiological mechanisms of exogenous NO and Spd with or without MB in increased chamomileplant tolerance to salinity stress.Comparing these responses can be useful in understanding the physiological and biochemical mechanisms of these compounds in plants which have to cope with salt stress.
MATERIALS AND METHODS
Chamomile (Matricaria recutita L.) seeds were sown in perlite and cocopite. After germination, seedlings were supplied with half strength of lang-Ashton nutrient solution three times a week. Sodium nitroprusside (SNP) was used as NO donor and methylene blue (MB) as inhibitor of NO
pathway. Two-month-old chamomileplants were obtained and watered separately with half strength Lang-Ashton solution with SNP (100^M), Spd (0.5mM), MB (100 nM), (MB + SNP) and (MB +Spd) for 10 days. Nutrient solution was used as control. After 10 days, all plants were divided in two groups one group was exposed to NaCl (200 mM) stress and other group was irrigated with water alone as control for 12 days.
Lipid peroxidation: For the measurement of lipid peroxidation in leaf rosettes, the thiobarbituric acid (TBA) test, which determines malondialdehyde (MDA) level, was applied (Heath and Packer, 1968).
H2O2 content: Hydrogen peroxide levels were determined according to Alexieva et al., (2001).
Enzyme extraction and antioxidant enzyme activity: Leaf fresh samples (500 mg) were ground in 5 ml of 50mM phosphate buffer (pH 7.5) containing 1mM EDTA, 1mM PMSF and 1% PVP using pre-chilled mortar and pestle. Then, the extract was centrifuged at 4 °C at 15,000 g for 30 min. The supernatant was used for measurements of enzyme activity and the activities of enzymes expressed as Unit/mg protein-1 (Bradford, 1976). Ascorbateperoxidase activity was measured by monitoring the oxidation of ascorbic acid (Nakano and Asada, 1981) and GPX activity was determined using the method of Plewa et al. ,(1999).
Statistical analysis: Data are means± SE of three replicates. Statistic assays were carried out by oneway ANOVA using Duncan test to evaluate whether the means were significantly different, taking p< 0.05 as significant.
RESULTS
Growth parameters: Salt stress (NaCl)
significantly decreased length of both shoot and
root of M. recutita plants (Figure 1). Pretreatment of plants with SNP and Spm significantly enhanced the growth of chamomile plants under saline condition. However, pretreatment of plants with (SNP+ MB), and or (Spm +MB) had the same effects on growth parameters under salinity stress when compared with SNP or Spm pretreated plants. In the non-saline conditions, exogenously applied SNP, Spm had no significant effect on length of shoot and root.
MDA and H2O2 content: results in Figure 2 demonstrated that salt stress, significantly increased the content of MDA. Under salt stress lipid peroxidation decreased in plants which were pretreated with SNP and Spd when compared with non-pretreated plants. Application of SNP or Spd with MB as pretreatment mitigates the effects of SNP and Spm. The results showed that salt stress increased H2O2 content, whereas SNP and Spd treatment significantly decreased the amounts of H2O2 in leaf rosettes of M. recutita. Exogenous application of (MB + SNP) and (MB + Spd) during 200mM NaCl had no significant effect on H2O2 content.
APX and GPX activites: the effect of salt stress on GPX and APX in chamomile plant leaves, either with or without SNP and Spd pretreatment was assayed. As is shown in (Fig. 3) the activity of GPX (Fig. 3-A) and APX (Fig. 3-B) was higher in stressed plants than those of the control groups, which may be a reflection of the oxidative burst under salt stress and the key role of these enzymes in ROS detoxification under these conditions. SNP pretreatment had no significant effects on GPX activity, while pretreatment of plants with Spd significantly enhanced the activity of this enzyme under stress condition. As shown in Figure 3-B, the activity of APX enzyme was considerably increased
in response to salinity stress. Application of SNP pretreatment increased the activity of APX in salt stressed plants while the Spd pretreatment increased the APX activity in control plant and had no significant effect on activity of this enzyme in
stress condition. Application of MB with SNP or Spd as pretreatments decreased the positive effects of these compounds on enzymes activity in saline conditions.
Figure 1. Effect of SNP, Spd and MB treatments on the length of shoot (A) and root (B) in chamomile plant leaves under control and salt stress condition. Data are means ± SE of three replicates. The significant of different between treatments was determined by one-way ANOVA taking p<0.05 as significant.
Figure 2. Effect of SNP, Spd and MB treatments on the contents of MDA (A) and H2O2 (B) in chamomile plant leaves under control and salt stress condition. Data are means± SE of three replicates. The significant of different between treatments was determined by one-way ANOVA taking p<0.05 as significant
Figure 3. Effect of SNP, Spd and MB treatments on the activity of GPX (A) and APX (B) activity in chamomile plant leaves under control and salt stress condition. Data are means ± SE of three replicates. The significant of different between treatments was determined by one-way ANOVA taking p<0.05 as significant.
DISCUSSION injury was time dependent and increased with
duration of stress. In the present investigations,
Salt stress disturbs intracellular ion homeostasis of
NaCl significantly increased the MDA content, while
plants, which leads to membrane dysfunction,
SNP and Spd alleviate the adverse effect of NaCl on
attenuation of metabolic activity, and cause growth
MDA concentration in chamomile plants. A
inhibition and ultimately leads to cell death
protective effect of NO on membrane injury has
(Sheokand et al., 2010). A key factor limiting plant
been reported under salt (Zhao et al., 2004),
growth is excessive Na+, a harmful mineral element
drought (Nasibi and Kalantari, 2009) and heavy
not required by most plants. Our finding showed
metal stress (Singh et al., 2008). It has been
that salinity stress has the negative effects on shoot
reported that role of NO in suppression of lipid
and root length and pre-treatment with SNP or Spd
peroxidation probably is related to NO reaction with
decreased NaCl damages, supporting that NO and
radicals of lipid alcoxyl (LO) and lipidperoxyl (LOO)
Spd is actively involved in the regulation of plant
that suppressed chain of peroxidation (Beligni and
growth. However, application of SNP and Spd with
Lamattina, 1999), that compatibility with results of
MB declined the improvement effect of SNP and
this experiment about reduction of MDA content by
Spd on plant growth under salinity stress. Previous
NO pretreatment. The role of polyamines in decline
studies have demonstrated that the exogenous NO
of MDA content also had been reported in some of
and polyamines mitigated decrease in plant growth
plants under stress condition (Velikova et al., 2000
caused by salinity is through increasing antioxidant
and Tang and Newton, 2005). The defensive role of
system, alleviating oxidative damage (Shi et al.,
polyamines may be related to the nature of these
2007; Zheng et al., 2011) and stimulating vacuolar
compounds, which can act as anti- oxidant, snatcher
H+-ATPase and H+-PPase activities (Liu et al., 2006)
of free radicals and membrane fixator (Velikova et
etc. Salt stress induces lipid peroxidation by
al., 2000). In this investigation, pretreatment of
production of ROS (Shi et al., 2007 and Zhang et al.,
plants with combination of SNP with MB decrease
2004), thus making the membranes leaky as evinced
the defensive effect of NO on lipid peroxidation, but
by increase electrolyte leakage. The membrane
in plant which were pretreated with (Spd+ MB),
MDA content did not change significantly in comparison with plants treated with Spd. Reduction of defensive effect of NO on lipid peroxidation by MB presumably concerned to the effect of MB on inhibition of signaling pathway of NO. Under normal conditions, the total amount of ROS formed in the plants is determined by the balance between the multiple ROS producing pathways and the ability of the enzymatic and non-enzymatic mechanism to deal with them. Under stress conditions, ROS formation is higher than ability of plants to remove it, and this could result in oxidative damages (Laspina et al., 2005). In chamomileplants under salinity, APX and GPX activities were elevated over the controls,therefore, we can assume that the plant antioxidant machinery was effectively struggling against stressful condition. Relatively higher activities of ROS-scavenging enzymes have been reported in tolerant genotypes when compared to susceptible ones, suggesting that the antioxidant system plays an important role in plant tolerance against environmental stresses (Shi et al., 2007). In addition, the results showed that under salt stress H2O2 content increased (Fig. 2). Increment of H2O2 content under salt stress was reported in response to salt stress in previous research (Uchida et al., 2002). Pretreatment with SNP and Spd decreased the H2O2 content and alleviated the salinity stress. In this study, the activity of GPX enzyme was significantly increased by exogenous Spd treatment, while SNP pretreatment is caused significant increase the activity of APX activity. In chamomile plants that exposed salt stress, the combination of MB with SNP (MB + SNP) and Spd (MB + Spd) had no significant effect on GPX activity, but (MB + Spd) treatment caused significant increase APX activity compared to plants treated with Spd alone. A
protective role of NO on H2O2 content has been reported under water stress (Zhao et al., 2008), salt stress (Sheokand et al., 2010) and heavy metal stress (Singh et al., 2008). It was suggested when NO/O2- proportion is in favor of NO, superoxide anion (O2-) combines with NO and produces peroxynitrite (ONOO), thus there is no superoxide anion to convert to H2O2, finally the amount of H2O2 will decrease in the presence of exogenous NO (Dellendonne et al., 2001). Peroxynitrite has been shown to combine with H2O2 to produce nitrite ion and oxygen (Beligni and Lamattina, 2001). In many studies, it was found that the function of PA alleviation of oxidative stress was attributed to induction of various ROS-scavenging enzyme activities (Hsu and Kao 2007; Wang et al., 2007; Tang et al., 2005). Combination of MB with SNP (SNP + MB) and Spd (Spd + MB) had not significant effect on H2O2 content in chamomile plants under stress condition. The result of this study indicated that MB could partially and entirely abolish the protective effect of NO on some physiological parameters under stress condition. Methylene blue also could reduce the alleviation effect of Spd on some of parameters in chamomile plant under salt stress. Comparing the effects of NO and SNP in this study showed that Spd probably acts through NO pathway, but the use of PTIO(scavenger of NO) is better to prove.
REFERENCES
Alexieva, V., Sergiev, I., Mapelli, S. and Karanov, E. (2001) The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ., 24, 1337- 1344.
Beligni, M.V. and Lamattina, L. (2001) Nitric oxide in plants: the history is just beginning. J.Plant Cell Environ., 24, 267-278.
221
Beligni, M.V. and Lamattina, L. (1999) Nitric oxide counteract cytotoxic processes mediated by rective oxygen species in plant tissues. Planta., 208, 337-344.
Bradford, M.M.( 1976) Arapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. J. Anal. Biochem., 72, 248-254.
Delledonne, M., Zeier, J., Marocco, A. and Lamb, C. (2001) Signal interactions between nitric ixide and reactive oxygen intermediates in the plant hypersensitive disease-resistance response. Proceed. Nat. Acad. Sci. USA, 98, 13454-13459.
Del Rio, L.A., Corpas, F.J. and Barroso, J.B. (2004) Nitric oxide and nitric oxide synthase activity in plants. Phytochem., 65, 783-792.
Fan, H.F., Guo, S.R., Li, J., Du, C.X. and Huang, B.J. (2007) Effects of exigenous nitric oxide on cucumis sativus seedlings growth and osmoatic adjustment substances contents under NaCl stress. J. Chin. Ecol., 26, 2045-2050.
Foyer, C.H. and Noctor, G. (2000) Oxygen processing in photosynthesis: regulation and signaling. New Phytol., 146, 359-388.
Heath, R.L. and Packer, L. (1968) Photoperoxidation in isolated chloroplast, kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 125, 189-198.
Hsu, Y. and Kao, C. (2007) Cadmium-induced oxidative damage in rice leaves is reduced by polyamines. Plant Soil,. 291, 27-37.
Huang, X., Rad, U. and Durner, J. (2002) Nitric oxide induces transcriptional activation of the nitric oxide-tolerant alternative oxidase in Arabidopsis suspension cells. Planta., 215, 914923.
Keaney, J.F., Puyana, J.C., Francis, S., Loscalzo, J.F., Stamler, J.S. and Loscalzo, J. (1994) Methylene blue reverse endotoxin-induced hypotension. J. Circ. Res., 74, 1121-1125.
Kopyra, M. and Gwozdz, E.A. (2004) The role of nitric oxide in plant growth regulation and responses to abiotic stresses. Acta. Physiol. Plant., 26, 459-472.
Kumar, D. and Klessig, D.F. (2000) Differential induction of tobacco MAP kinases by the defense signals nitric oxide, salicylic acid, ethylene and jasmonic acid. Mol. Plant. Microbe. Interact., 13(3), 347-351.
Lamotte, O., Courtois, C., Barnavon, L., Pugin, A. Wendehenne, D. (2005) Nitric oxide in plants: the biosynthesis and cellsignaling properties of a fascinating molecul. Planta., 221, 1-4.
Laspina, N.V., Groppa, M.D., Tomaro, M.L. and Benavides, M.P. (2005) Nitric oxide protects sunflower leaves against Cd-induced oxidative stress. Plant. Sci., 169, 323-330.
Liu, J., Yu, B. and Liu, Y. (2006) Effects of spermidine and spermine levels on salt tolerance associated with tonoplast H+-ATP ase and H+-PPase activities in barley roots. Plant. Growth. Regul., 49, 119-126.
Mata, C.G. and Lamattina, L. (2001) Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress. Plant. Physiol., 126, 1196-1204.
McCue, K.F. and Hanson, A.D. (1992) Salt-inducible betaine aldehyde dehydrogenase from sugar beet: cDNA cloning and expression. Plant Mol Biol., 18, 1-11
Nakano, Y. and Asada, K. (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase
in spinach choloroplast. Plant. Cell. Physiol., 22, 867-880.
Nasibi, F. and Kalantari, Kh. (2009) Influence of nitric oxide in protection of tomato seedling against oxidative stress induced by osmotic stress. Acta. Physiol. Plant., 31, 1037-1044.
Neill, S., Desikan, R. and Hancock, J. (2003) Nitric oxide signaling in plant. New Phytol., 159, 1135.
Paciullo, C.A., McMahon Horner, D., Hatton, K.W., Flynn, J.D. (2010) Methylene blue for the treatment of septic shock. Pharmacotherapy, 30, 702-715.
Plewa, M.J., Smith, S.R. and Wanger, E.D. (1991) Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobaccocell peroxidase. Mutat. Res., 247, 57-64.
Sheokand, S., Bhankar, V. and Sawhney, V. (2010) Ameliorative effect of exogenous nitric oxide on oxidative metabolism in NaCl treated Chickpea plants. Braz. J. Plant Physiol., 22 (2), 81-90.
Shi, Q., Ding, F., Wang, X. and Wei, M. (2007) Exogenous nitric oxide protect cucumber roots against oxidative stress induced by salt stress. Plant Physiol. Biochem., 45(8), 542-550
Singh, H.P., Batish, D.R., Kaur, G., Arora, K. and Kohli, R.K. (2008) Nitric oxide (as sodium nitroprusside) supplemention ameliorates Cd toxicity in hydroponically grown wheat roots. Env. Exp. Bot., 63(1), 158-167.
Stamler, J.S. (1994) Redox signaling: nitrosylation and related target interactions of nitric oxide. Cell, 78(6), 931-936.
Tabor, C.W. and Tabor, H. (1984) Polyamines. Annu. Rev. Biochem., 53, 749-790.
Tang, W. and Newton, R.J. (2005) Polyamines reduce salt-induced oxidative damage by increasing the activities of antioxidant enzymes and decreasing lipid peroxidation in Virginia pine. Plant Growth Regul., 46, 31-43
Tun, N.N., Santa-Catarina, C., Begum, T., Silveira,V., Handro, W., Floh, E. and Scherer, G. (2006) Polyamines induce rapid biosynthesis of nitric oxide (NO) in Arabidopsis thaliana seedlings. Plant Cell Physiol., 47(3), 346-354.
Uchida A.; Jagendorf A.T.; Hibino T.; Takabe T.; Takabe T. (2002) Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice. Plant Sci., 163(3), 515-523.
Velikova, V., Yordanov, I. and Edreva, A. (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci., 151, 5966.
Wang, X., Shi, G., Xu, Q. and Hu, J. (2007) Exogenous polyamines enhance copper tolerance of Nymphoids peltatum. J. Plant Physiol., 164(8), 1062-1070.
Zhao, L., Zhang, F., Guo, J., Yang, Y., Li, B. and Zhang, L. (2004) Nitric oxide functions as a signal in salt resistance in the calluses from two ecotypes of Reed. Plant Physiol., 134(2), 849-857.
Zhao, H. and Yang, H. (2008) Exogenous polyamines alleviate the lipid peroxidation induced by Cadmium chloride stress in Malus Hupehensis Rehd. Sci. Hort., 116, 442-447
Zhao, L., He, J., Wang, X. and Zhang, L. (2008) Nitric oxide protects against polyethyleneglycol-induced oxidative damage in two ecotypes of
reed suspension cultures. J. Plant Physiol., Zheng, C.L., Liu, L. and Xu, G.Q. (2011) The
165(2), 849-857. physiological responses of carnation cut
flowers to exogenous nitric oxide. Sci. Hortic., 127(3), 424-430.
