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Journal of Stress Physiology & Biochemistry, Vol. 9 No. 4 2013, pp. 180-186 ISSN 1997-0838 Original Text Copyright © 2013 by Zhu, Di, Jia, Zhang, Wang
ORIGINAL ARTICLE
Comparison of Nonshivering Thermogenesis Induced by Norepinephrine Stress in Tree Shrews
Wan-long Zhu 1#, Di Zhang Jia Zheng 1#, Lin ZHang Jin-hu Liu 2, Jin Cheng 2, Zheng-kun Wang 1*
1 School of life Science of Yunnan Normal University, Kunming, 650500, China
2 Pingliang medical college, PingLiang, 744000, China
# Zhu WL and Zheng J contributed equally to this work
Tel.: +86 0871 5516068 *E-Mail: [email protected]
Received July 1, 2013
Nonshivering thermogenesis (NST)is an important thermogenic mechanism for small mammals. Quantitative measurement of NST is usually stimulated by injection of norepinephrine. The injection dosage of norepinephrine (NE)is critical for eliciting the maximum NST. Three empirical equations of NE dosages were often referenced in previous studies: (1)NE (mg / kg)= 2.53W-04; (2)NE(mg/kg) = 3.3W-0458 and (3)NE(mg/kg)= 6.6W-0458. In the present study, we used tree shrews (Tupaia belangeri) as experiment subjects to test the effects of the three dosages of NE on NST. Results showed that all the three dosages of NE could induce the maximum NST for T. belangeri. No significant differences were found in NST among groups and the NST was respectively 2.63±0.12 (formula 1), 2.66±0. 11 (formula 2) and 2.78±0.15 (formula 3). However, when injected with NE dosage from formula 3, the increase of body temperatures was significantly higher than the other two NE dosages (increased 1.5±0. 1oC (formula 3), 0.8±0. 2oC (formula 2), and 0.6±0. 1oC (formula 1), respectively). In order to prevent the death because of hyperthermia, formula 1 or 2 is recommended to be used.
Key words: Tupaia belangeri; Nonshivering thermogenesis; Norepinephrine
ORIGINAL ARTICLE
Comparison of Nonshivering Thermogenesis Induced by Norepinephrine Stress in Tree Shrews
Wan-long Zhu 1#, Di Zhang \ Jia Zheng 1#, Lin ZHang \ Jin-hu Liu 2, Jin Cheng 2, Zheng-kun Wang 1*
1 School of life Science of Yunnan Normal University, Kunming, 650500, China
2 Pingliang medical college, PingLiang, 744000, China
# Zhu WL and Zheng J contributed equally to this work
Tel.: +86 0871 5516068 *E-Mail: [email protected]
Received July 1, 2013
Nonshivering thermogenesis (NST)is an important thermogenic mechanism for small mammals. Quantitative measurement of NST is usually stimulated by injection of norepinephrine. The injection dosage of norepinephrine (NE)is critical for eliciting the maximum NST. Three empirical equations of NE dosages were often referenced in previous studies: (1)NE (mg / kg)= 2.53W-04;
(2)NE(mg/kg) = 3.3W-0458 and (3)NE(mg/kg)= 6.6W-0458. In the present study, we used tree shrews (Tupaia belangeri) as experiment subjects to test the effects of the three dosages of NE on NST. Results showed that all the three dosages of NE could induce the maximum NST for T. belangeri. No significant differences were found in NST among groups and the NST was respectively 2.63±0.12 (formula 1), 2.66±0. 11 (formula 2) and 2.78±0.15 (formula 3). However, when injected with NE dosage from formula 3, the increase of body temperatures was significantly higher than the other two NE dosages (increased 1.5±0. 1oC (formula 3), 0.8±0. 2oC (formula 2), and 0.6±0. 1oC (formula 1), respectively). In order to prevent the death because of hyperthermia, formula 1 or 2 is recommended to be used.
Key words: Tupaia belangeri; Nonshivering thermogenesis; Norepinephrine
Non-shivering thermogenesis occurs in brown adipose tissue (brown fat) that is present in all mammals, brown adipose tissue has a unique protein (uncoupling protein-1) that allows the uncoupling of electrons moving down their mitochondrial gradient from the synthesis of ATP, thus allowing the energy to be dissipated as heat (Jansky, 1973). Non-shivering thermogenesis is regulated mainly by thyroid hormone and the
sympathetic nervous system. Some hormones, such as norepinephrine (NE), may stimulate thermogenesis by activating the sympathetic nervous system, Because NE induced NST by heat is equivalent to that induced by cold, therefore, the maximum NST is generally defined as the total metabolic responses to NE, which can injecte the NE dose related to the body mass, determination of animal in the following thermal neutral zone in a
quiet, sober condition to determine the maximum oxygen consumption (Heldmaier et al., 1982).
Scholars focus on NST induced by different doses of NE on small mammals for many years, but only selected by the optimal NE injection dose obtain accurate and reliable results for NST. To determine appropriate doses of NE injection, which need a set of the different dose gradient, making dose response curve, and also need large amount of samples. Therefore, some scholars try to establish relations of NE injection dose related to body mass in small mammals. At present, there are
3 dose calculation formula in the literature: (1)NE(mg/kg)= 2.53W-04 (Wunder and Gettinger, 1996); (2)NE(mg/kg) = 3.3W-0458 (Merritt, 1986) and
(3)NE(mg/kg)= 6.6W-0458 (Heldmaier, 1971). These 3 kinds of doses are applied, and they can induce the maximum NST capacity, but there is not a systematic evaluation of the 3 NE dose.
Tupaia belangeri (Mammalia: Scandentia: Tupaiidae) live at the highest latitude, with the Yunnan-Kweichow Plateau being its northern limit (Wang et al., 1991). Previous studies demonstrate that environmental factors, such as short photoperiods and cold, are effective cues that influence body mass and thermogenesis in T. belangeri, separately (Zhang et al., 2011; 2012a; 2012b; 2012c). T. belangeri shown a seasonal increased in body mass and thermogenic capacity to adapt to the increase of energy requirements for thermoregulation (Zhu et al., 2012). In the present study, we determined the optimal NE dose induced by NST by comparing the 3 dose calculation formula.
MATERIALS AND METHODS
Samples
T. belangeri were captured (25°25 -26°22' N,
102°13'-102°57' E, 1679 m in altitude) around boscage at Luquan County in 2012. The average yearly temperature was 15.6 °C, mean monthly temperature ranges from 7.8 °C in winter to 19.6 °C in summer. After being captured, T. belangeri were brought and bred at the School of Life Sciences, Yunnan Normal University, Kunming (1910 m in altitude). Each weight-matched tree shrew was housed individually in a wire cage (40 cmx40 cmx40 cm) with no bedding; all animals (60 males) were healthy adults. The photoperiod, ambient temperature and humidity were maintained at 12 L: 12D (light on at 0800 h), 25 (±1)°C , and 850/-920/ relative humidity, respectively. Animals were kept for at least two weeks, and 10 adults with similar body mass were measured. 3 kinds of NE dose according to experiment the sequence from low to high: (1)NE (mg / kg ) = 2.53W"0-4; (2)NE(mg/kg) = 3.3W-0'458 and (3)NE(mg/kg)= 6.6W0458. Injection interval different dose is 3 day in order to avoid the effect of NE on metabolism (Wang and Wang, 2006). Pregnant, lactating or young individuals were excluded.
Measurement of metabolic rates
Metabolic rates were measured by using AD ML870 open respirometer (AD Instruments, Australia) at 30°C within the TNZ (thermal neutral zone), gas analysis were using ML206 gas analysis instrument, the temperature was controlled by SPX-300 artificial climatic engine (±0.5°C), the metabolic chamber volume is 500 ml, flow is 200 ml/min. The tree shrew were stabilized in the metabolic chamber for at least 60 min prior to the RMR measurement, oxygen consumption was recorded for more than 60 min at 5 min intervals. Two stable consecutive lowest readings were taken to calculate RMR (Li and Wang, 2005). Calculate method of metabolic rate is detailed by Hills (1972) (Hill,
1972). All metabolic measurements were performed more than 400 hours. Before and after the experiment, animal's body mass were measured, and body temperature were measured by digital thermometer (Sinan instrument of Beijing Normal University, SN2202), 1.5 cm was the probe inserted to the rectum, and read the body temperature after 45 s.
Nonshivering thermogenesis (NST) was induced by subcutaneous injection of norepinephrine (NE) (Shanghai Harvest Pharmaceutical Co. Ltd) and measured at 25°C. Two consecutive highest recordings of oxygen consumption more than 60 min at each measurement were taken to calculate the NST (Zhu et al., 2010).
Statistical analysis
Data were analyzed using SPSS 15.0 software package. Prior to all statistical analyses, data were examined for assumptions of normality and homogeneity of variance, using Kolmogorov-
Smirnov and Levene tests, respectively. Throughout the acclimation, NST were analyzed by Repeated measures ANCOVA with body mass as a covariate. Results were presented as mean ± SEM, and P < 0.05 was considered to be statistically significant.
RESULTS
Since no gender effects were found in any of the measured parameters, data from females and males were combined thereafter.
No differences of RMR were found between 3 kinds of NE does in T. belangeri (F=0.693, P>0.05, Table 1), there were also showed that No differences of RMR were found between 3 kinds of NE does (F=1.322, P>0.05, fig 1). For the body mass, there were no significant differences before and after the experiment (Table 1). Injected with NE dosage from formula 3, the increase of body temperatures was significantly higher than the other two NE dosages (increased 1.5±0. 1°C (formula 3), 0.8±0. 2°C (formula 2), and 0.6±0. 1°C (formula 1), respectively (fig 2).
Table 1. Body mass and resting metabolic rate (RMR) elicited by 3 different norepinephrine dosages in Tupaia belangeri
NE does Initial body mass (g) Final body mass (g) RMR (ml O2|g.h)
NE(mg | kg ) = 2.53W-04 102.32±3.21 100.63±3.22 1.23±0.21
NE(mg|kg) = 3.3W-0458 103.32±2.36 102.01±2.31 1.25±0.16
NE(mg|kg)= 6.6W-0458 101.32±2.32 99.25±2.33 1.28±0.25
Formulai Formula 2 Formula 3 Figure 1. NST elicited by 3 different norepinephrine dosages in Tupaia belangeri
Formula 2 Formula 3
Figure 2. Changes of body temperature elicited by 3 different norepinephrine dosages in Tupaia belangeri
DISCUSSION
NST is an important way of heat production in small mammals (Heldmaier et al., 1990). Nonshivering thermogenesis is regulated mainly by the sympathetic nervous system (Klingenberg and Echtay, 2001). Due to NST associated with NE does, the optimal NE injection dose obtain accurate NST (Depocas, 1960). When the injection of NE dose were too low, NE heat production contribution will not be fully displayed, at high doses induced thermogenesis remains unchanged or even drop, while the animal always lose the ability to regulate the body temperature because of hyperthermia, eventually led to the death (Wunder and Gettinger, 1996). Heldmaier (1971) proposed formula (3) is widely used in heat production researches (Li et al., 2001). But because of the experimental animal was under 5°C acclimation treatment, and the animal were anesthesia in the determination of NST, so it was generally believed that NE injection will cause the animal activity, and animal activity will affect the metabolism of determination, and later found anesthesia will produce some effects on metabolism, and after the injection of NE, animal often does not move. And many experiments were
not at 5C domestication, therefore, application of this dose formula is limited. Wunder and Gettinger studied wild small mammals which body weight were 15-900g , the experimental domestication was 23C, animal without anesthesia, the results showed that NE dose of acclimation temperature does not affect the induction of NST, but anesthesia will affect the NE sensitivity, and proposed formula (1) according to the dose response curve. In the present study, NST induced by 3 different norepinephrine dosages showed no significant differences in T. belangeri, which indicated that 3 kinds of injection dose can induce the maximum NST, but the changes of body temperature showed significant differences. Injected with higher NE dosage (formula 3), the increase of body temperatures was significantly higher than the other two NE dosages.
In conclusion, the present results suggested 3 doses can be used, we recommended dose (1) and (or) (2).
ACKNOWLEDGEMENT
The project was financially supported by National Science Foundation of China (No.
31260097); Project of Basic research for application
in Yunnan Province (No. 2013FA014).
REFERENCES
Depocas, F. (1960) The calorigenic response of cold-acclimated white rats to infused noradrenaline. Can J Biochem Physiol, 38: 107-114.
Jansky, L. (1973) Non-shivering thermogenesis and its thermoregulatory significance. Biol Rev, 48: 85-132.
Heldmaier, G. (1971) Nonshivering thermogenesis and body size in mammals. J Comp Physiol, 73: 222-248.
Heldmaier, G., Steinlechner, S., Rafael, J. (1982) Nonshivering thermogenesis and cold resistance during seasonal acclimatization in the Djungarian hamster. J Comp Physiol B, 149: 1-9.
Hill, R.W. (1972) Determination of oxygen consumption by use of the paramagnetic oxygen analyzer. J Appl. Physiol., 33(2): 261263.
Klingenberg, M., Echtay, K.S. (2001) Uncoupling proteins: the issues from a biochemist point of view. Biochim Biophys Acta, 1504: 128-143.
Li, Q.F., Sun, R.Y., Huang, C.X., Wang, Z.K., Liu, X.T., Hou, J.J. (2001) Cold adaptive thermogenesis in small mammals from different geographical zones of China. Comp Biochem Physiol, 129: 949-961.
Li, X.S., Wang, D.H. (2005) Regulation of body weight and thermogenesis in seasonally acclimatized Brandt's voles (Microtus brandti). Homones and Behavior, 48(3): 321-328.
Merritt, J. (1986) Winter survival adaptations of the short-tailed shrew (Blarina brevicauda) in an Appalachian montane forest. J Mammal, 67:
450-464.
Wang, Y.X., Li, C.Y., Ma, S.L. (1991) The classification and ecology of tree shrews. In: Peng, Y., Ye, Z., Zou, R. Eds. Biology of Chinese Tree shrews (Tupaia belangeri Chinensis). Yunnan Scientic and Technological Press, Kunming.
Wang, J.M., Wang, D.H. (2006) Comparison of nonshivering thermogenesis induced by dosages of norepinephrine from 3 allometric equations in Brandt' s voles (Lasiopodomys brandtii). Acta Theriol. Sin., 26(1): 84-88.
Wunder, B.A., Gettinger, R.D. (1996) Effects of body mass and temperature acclimation on the nonshivering thermogenic response of small mammals. In: Geiser, F., Hulbert, A.J., Nicol, S.C. eds. Adaptations to the cold: Tenth
international hibernation symposium, 131-139.
Zhang, L., Wang, R., Zhu, W., Liu, P., Cai, J., Wang, Z., Sivasakthivel, S., Lian, X. (2011) Adaptive thermogenesis of liver in tree shrew (Tupaia belangeri) during cold acclimation. Anim. Biol., 61: 385-401.
Zhang, L., Liu, P., Zhu, W., Cai, J., Wang, Z. (2012a) Variations in thermal physiology and energetics of the tree shrew (Tupaia belangeri) in response to cold acclimation. J. Comp. Physiol. B, 182: 167-176.
Zhang, L., Zhang, H., Zhu, W., Li, X., Wang, Z. (2012b) Energy metabolism, thermogenesis and body mass regulation in tree shrew (Tupaia belangeri) during subsequent cold and warm acclimation. Comp. Biochem. Physiol. A, 162: 437-442.
Zhang, L., Zhu, W.L., Wang, Z.K. (2012c) Role of photoperiod on hormone concentrations and adaptive capacity in tree shrews, Tupaia belangeri. Comp. Biochem. Physiol. A, 163: 253-
Zhu, W.L., Jia, T., Lian, X., Wang, Z.K. (2010) Effects of cold acclimation on body mass, serum leptin level, energy metabolism and thermognesis in Eothenomys miletus in Hengduan Mountains region. J. Therm. Biol., 35(1): 41-46.
Zhu, W.L., Zhang, H., Wang, Z.K. (2012). Seasonal changes in body mass and thermogenesis in tree shrews (Tupaia belangeri) the roles of photoperiod and cold. J. Therm. Biol., 37: 479484.
