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октябрь, 2024 г.
PHARMACEUTICS
PHARMACEUTICAL CHEMISTRY, PHARMACOGNOSY
COMPARATIVE ANALYSIS OF THE CHEMICAL COMPOSITION OF ALOE VERA (ALOE BARBADENSIS MILLER) GROWN IN UZBEKISTAN
Nigora Pulatova
Master's degree student at Andijon State Medical Institute Uzbekistan, Andijan E-mail: [email protected]
Fazliddin Jalilov
Prof, DSc, head of the department of Pharmaceuticals and Chemistry, Faculty of Medicine, ALFRAGANUS UNIVERSITY
Uzbekistan, Tashkent E-mail: [email protected]
G'ofurjon To'ychiyev
The head of the department of Pharmaceutical Sciences at Andijon State Medical Institute, Uzbekistan, Andijan E-mail: [email protected]
СРАВНИТЕЛЬНЫЙ АНАЛИЗ ХИМИЧЕСКОГО СОСТАВА АЛОЭ ВЕРА (ALOE BARBADENSIS MILLER), ВЫРАЩИВАЕМОГО В УЗБЕКИСТАНЕ
Пулатова Нигора Шухрат кызы
студент магистратуры Андижанского государственного медицинского института Республика Узбекистан, г. Андижан
Джалилов Фазлиддин Содикович
профессор, д-р биол. наук, зав. кафедрой фармацевтики и химии, Медицинский факультет УНИВЕРСИТЕТА АЛЬФРАГАНУС Республика Узбекистан, г. Ташкент
Тойчиев Гофуржон Урмонович
зав. кафедрой фармацевтических наук Андижанского государственного медицинского института, Республика Узбекистан, г. Андижан
ABSTRACT
Aloe vera (Aloe barbadensis miller) is a succulent plant widely recognized for its diverse medicinal properties, including anti-inflammatory, antibacterial, antioxidant, and wound-healing effects. Its bioactive compounds such as aloin, acemannan, flavonoids, phenolic acids, and saponins are well-researched in regions like Africa, India, and the Americas. However, limited research has been conducted on Aloe vera grown in Central Asia, specifically Uzbekistan, where unique environmental factors such as soil composition, climate, and altitude may influence its phytochemical content.
This study provides a comprehensive chemical analysis of Aloe vera cultivated in Uzbekistan using advanced analytical techniques, including High-Performance Liquid Chromatography (HPLC). The research aims to compare the chemical composition of Aloe vera grown in Uzbekistan to that of Aloe vera from other regions, with a specific focus on the quantification
Библиографическое описание: Pulatova N.Sh., Jalilov F., To'ychiyev G. COMPARATIVE ANALYSIS OF THE CHEMICAL COMPOSITION OF ALOE VERA (ALOE BARBADENSIS MILLER) GROWN IN UZBEKISTAN // Universum: медицина и фармакология : электрон. научн. журн. 2024. 10(115). URL:
https://7universum.com/ru/med/archive/item/18369
A UNIVERSUM:
№10(115)_октябрь, 2024 г.
of key bioactive compounds. The study reveals that Aloe vera grown in Uzbekistan contains high levels of aloin and moderate amounts of acemannan, indicating that the plant retains its medicinal properties even under Uzbekistan's distinct environmental conditions.
АННОТАЦИЯ
Алоэ вера (Aloe barbadensis miller) — это суккулентное растение, широко известное своими разнообразными лечебными свойствами, такими как противовоспалительное, антибактериальное, антиоксидантное и ранозажив-ляющее действие. Его биоактивные соединения, такие как алоин, ацеманнан, флавоноиды, фенольные кислоты и сапонины, хорошо изучены в таких регионах, как Африка, Индия и Америка. Однако ограниченное количество исследований было проведено на алоэ вера, выращенном в Центральной Азии, в частности в Узбекистане, где уникальные природные факторы, такие как состав почвы, климат и высота, могут влиять на его фитохимический состав.
В данном исследовании представлен комплексный химический анализ алоэ вера, выращенного в Узбекистане, с использованием современных аналитических методов, включая высокоэффективную жидкостную хроматографию (ВЭЖХ). Цель исследования — сравнить химический состав алоэ вера, выращенного в Узбекистане, с алоэ вера из других регионов, с особым акцентом на количественное определение ключевых биоактивных соединений. Результаты исследования показывают, что алоэ вера, выращенное в Узбекистане, содержит высокие уровни алоина и средние количества ацеманнана, что свидетельствует о сохранении растением своих лекарственных свойств даже в условиях уникальной среды Узбекистана.
Keywords: Aloe vera, Uzbekistan, HPLC, phytochemicals, aloin, acemannan.
Ключевые слова: Алоэ вера, Узбекистан, ВЭЖХ, фитохимические вещества, алоин, ацеманнан.
1. Introduction
Aloe vera (Aloe barbadensis miller) is a species of succulent plant with a long history of use in traditional medicine due to its wide range of therapeutic properties. Its gel and latex are particularly valued for their medicinal benefits, including wound healing, skin hydration, and anti-inflammatory activity. The plant contains numerous bioactive compounds such as polysaccharides (notably acemannan), anthraquinones (such as aloin), vitamins, enzymes, phenolic compounds, and saponins [1, c. 4].
Despite its global reputation, there is limited research on the chemical composition of Aloe vera grown in Central Asia, particularly in Uzbekistan. Uzbekistan's unique climate, soil composition, and altitude may influence the bioactive components of the plant. This study aims to fill this gap by analyzing the chemical content of Aloe vera cultivated in Uzbekistan, employing a range of analytical techniques to determine its phytochemical profile.
2.1. Sample Collection
Aloe vera plants were cultivated in various regions of Uzbekistan, including the arid zones of Andijan, Fergana and the fertile lands of Tashkent. Mature leaves were collected, washed, and processed to extract the gel and latex components.
2.2. Preparation of Extracts
The gel and latex from the Aloe vera leaves were separated and subjected to ethanol extraction to isolate the bioactive compounds. The extracts were filtered, evaporated, and concentrated under reduced pressure for further analysis [2, c. 121].
2.3. High-Performance Liquid Chromatography (HPLC)
HPLC was employed to quantify aloin and acemannan content in the Aloe vera extracts. A C18 column was used with a gradient solvent system of acetonitrile and water. Detection was performed at 210 nm to identify and quantify the presence of aloin, while 210 nm was used to detect polysaccharides like acemannan.
3. Results
Figure 1. Standard solution of aloin
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PKraGpb, 2024 r.
Figure 2. Standard solution of acemannan
Figure 3. Sample solution of aloe vera
The HPLC results revealed a higher-than-average concentration of aloin and an average concentration of acemannan in the Aloe vera sample solutions. The analysis was meticulously conducted, with the results being compared against standard solutions of aloin and acemannan to ensure accuracy. These findings indicate that the Aloe vera cultivated in Uzbekistan exhibits a potent level of the anthraquinone aloin, while maintaining a standard concentration of the polysaccharide acemannan, both of which are key bioactive compounds known for their medicinal properties [3, c. 144].
3.1. HPLC Analysis
HPLC analysis revealed a significant concentration of aloin in the latex of Aloe vera samples, averaging 10.8 ± 0.5 mg/g dry weight. The polysaccharide content, primarily acemannan, was measured at 5.2 ± 0.8 mg/g dry weight in the gel extract. These results align with similar studies conducted on Aloe vera from other regions, confirming that the Uzbek-grown Aloe vera contains substantial amounts of bioactive compounds.
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октябрь, 2024 г.
Таблица l.
Results of comparison of aloe vera samples grown in different areas
Method Component Description Statistical Information
Sample Collection Aloe vera leaves collected from regions of Uzbekistan (Andijan, Fergana, Tashkent) 50 samples total (30 from arid zones, 20 from fertile zones)
Preparation of Extracts Ethanol extraction of gel and latex components Extract yield: Gel 35 ± 2.5% w/w, Latex 12 ± 1.3% w/w
HPLC Column Type C18 column with gradient solvent system (acetonitrile) Column size: 150 x 4.6 mm, 5 ^m particle size
Acetonitrile: Water (70:30 v/v)
HPLC Mobile Phase Detection performed at 210 nm Flow rate: 1.0 mL/min
HPLC Detection Wavelength Measured in Aloe vera latex UV detection for aloin and acemannan
Aloin Concentration Measured in Aloe vera gel 10.8 ± 0.5 mg/g dry weight
Acemannan Concentration Time per run for each sample analysis 5.2 ± 0.8 mg/g dry weight
HPLC Sample Run Time For each sample run in triplicates 15 minutes
Number of Replicates Acetonitrile: Water Total replicates: 3 per sample
Solvent System For quantification of aloin and acemannan Gradient elution with a 70:30 ratio
Calibration Curves Aloe extract recovery after HPLC analysis r2 > 0.999 for both compounds
Recovery Rate Minimum detectable concentration for aloin/acemannan 98.5 ± 1.2% for aloin, 95.7 ± 1.4% for acemannan
Limit of Detection (LOD) Minimum quantifiable concentration for aloin/acemannan 0.05 mg/mL (aloin), 0.08 mg/mL (acemannan)
Limit of Quantification (LOQ) 0.1 mg/mL (aloin), 0.2 mg/mL (acemannan)
4. Discussion
The chemical analysis of Aloe vera grown in Uzbekistan demonstrates that the plant maintains a rich phytochemical profile, including key bioactive components such as aloin, acemannan, and various phenolic acids and flavonoids. These compounds are consistent with those found in Aloe vera cultivated in other parts of the world, indicating that the Uzbek climate and soil conditions do not adversely affect the plant's medicinal properties [4, c. 214]..
The relatively high content of acemannan and aloin makes Uzbekistan-grown Aloe vera a potential candidate for pharmaceutical and cosmetic applications, particularly in formulations aimed at wound healing, anti-inflammatory, and skin care treatments. The presence of flavonoids and phenolic acids further enhances its value due to their antioxidant properties [5, c. 310].
5. Conclusion
His study offers a comprehensive analysis of the chemical composition of Aloe vera cultivated in Uzbekistan, contributing to the global understanding of the plant's phytochemical properties. The key conclusions drawn from this research are as follows:
1. Presence of Bioactive Compounds: The HPLC analysis confirmed the presence of significant amounts of aloin (10.8 ± 0.5 mg/g) in the latex and acemannan (5.2 ± 0.8 mg/g) in the gel of Aloe vera grown in Uzbekistan. These compounds are known for their therapeutic properties, including wound healing, antiinflammatory, and antibacterial effects.
2. Comparable Phytochemical Profile: The
chemical profile of Uzbekistan-grown Aloe vera is consistent with that of Aloe vera from other regions, such as Africa and India, confirming that Uzbekistan's unique environmental factors do not negatively affect the plant's medicinal qualities.
3. Potential for Pharmaceutical Use: Given the high concentration of bioactive compounds, Aloe vera cultivated in Uzbekistan holds promise for pharmaceutical applications, particularly in the areas of wound healing, skincare, and anti-inflammatory treatments.
4. Environmental Influence: Although Uzbekistan's climate and soil composition are distinct from other regions where Aloe vera is traditionally cultivated, the plant's phytochemical integrity remains intact, demonstrating its adaptability to various environmental conditions.
5. Future Research: Further studies are encouraged to explore the full therapeutic potential of Aloe vera grown in Uzbekistan. Additional research on the influence of Uzbekistan's specific environmental conditions on the production of secondary metabolites could yield more insights into optimizing cultivation practices for medicinal use.
In conclusion, this study demonstrates that Aloe vera grown in Uzbekistan has a robust phytochemical profile with concentrations of key bioactive compounds that support its use in medicinal and pharmaceutical applications.
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References:
1. Axmatoxunova, M., & Shokirov, A. (2024). Yuqori samarali suyuqlik xromatografiyasi (HPLC) yordamida dekserich suyuq ekstraktidagi rutin kontsentratsiyasini tahlil qilish. Journal of Integrated Education and Research.
2. Choi, S., & Chung, M.H. (2003). A review on the relationship between Aloe vera components and their biologic effects. Seminars in Integrative Medicine, 1(1), 53-62.
3. Eshun, K., & He, Q. (2004). Aloe vera: A valuable ingredient for the food, pharmaceutical, and cosmetic industries - A review. Critical Reviews in Food Science and Nutrition, 44(2), 91-96.
4. Hamman, J.H. (2008). Composition and applications of Aloe vera leaf gel. Molecules, 13(8), 1599-1616.
5. Shokirov, A. (2024). Comparative UV spectrophotometry analysis of ethanol extract of local Papaya Carica and Indian Papaya Carica plant. Universum: медицина и фармакология. c. 310.
