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1Colloidal stability of gold nanoparticles conjugates with lysozyme under the influence of both environmental acidity and
temperature factors
E. Molkova*, V. Pustovoy, R. Sarimov, S. Gudkov, A. Simakin, E. Nagaev, T. Matveeva
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova Str. Moscow, Russia
* bronkos627@gmail.com
Upon entering the biological environment, any nanoscale systems instantly bind proteins that form a "protein crown" [1]. The protein corona is formed due to the thermodynamics of the aqueous environment and is mediated by Coulomb and van der Waals forces, hydrogen bonds and hydrophobic interactions [2]. The stability of such agglomerates can be influenced by a large number of factors. The absence of reliable knowledge about the effect of the resulting protein corona on further interactions of nanoparticles with the environment is a serious problem [3]. In our previous work, we considered the factor of the influence of the acidity of the medium on aggregation - disaggregation [4]. Since not one factor affects any process, but several, the next step in studying the colloidal stability of the nanosystem "lysozyme + gold nanoparticles" is to investigate the influence of the acidity of the medium coupled with temperature exposure. The measurements were carried out in the pH range from 2 to 12 and at a temperature from 25 to 80°C. The choice of the upper temperature limit is determined by the melting point of the protein lysozyme. Optical research methods were chosen as the fastest and most convenient for studying the states of a colloidal solution.
[1] R. Cai and C. Chen, The Crown and the Scepter: Roles of the Protein Corona in Nanomedicine, Advanced Materials, 1805740, (2018).
[2] T. Kopac, Protein corona, understanding the nanoparticle-protein interactions and future perspectives: A critical review, International Journal of Biological Macromolecules, 169, 290-301, (2021).
[3] J. Gebauer, M. Malissek, S. Simon, S. Knauer, M. Maskos, R. Stauber, L. Treuel, Impact of the Nanoparticle-Protein Corona on Colloidal Stability and Protein Structure. Langmuir, 28(25), 9673-9679, (2012).
[4] E. Molkova, V. Pustovoy, E. Stepanova, I. Gorudko, M. Astashev, A. Simakin, R. Sarimov, S. Gudkov, pH-Dependent HEWL-AuNPs Interactions: Optical Study, Molecules, 29, 82, (2024).
