Научная статья на тему 'Characterization of self-organized clusters of protein-coated Au nanoparticles in water'

Characterization of self-organized clusters of protein-coated Au nanoparticles in water Текст научной статьи по специальности «Нанотехнологии»

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Текст научной работы на тему «Characterization of self-organized clusters of protein-coated Au nanoparticles in water»

The 30th International Conference on Advanced Laser Technologies B-P-7

ALT'23

Characterization of self-organized clusters of protein-coated Au

nanoparticles in water

E. Molkova, B. Sarimov, S.Gudkov, V. Pustovoy, A.Semakin

Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova Str. Moscow, Russia

bronkos627@gmail.com

Nanoparticles trapped in the physiological fluid can be considered as systems that are formed as a result of successive interactions with the structures of the medium. The phenomenon of the formation of a "protein crown" on the surface of nanoparticles due to dynamic physicochemical interactions is known [1, 2].

Gold nanoparticles have important physical properties, such as surface plasmon resonance and the ability to quench fluorescence, which is successfully used in numerous test systems [3].

Lysozyme is widely used in the food industry. An increase in the content of lysozyme in biological fluids serves as a signal for certain diseases such as meningitis, blood and kidney diseases [4, 5]. Therefore, there is often a need for qualitative and quantitative characterization of lysozyme in liquid samples using biochemical test systems, including the use of nanoparticles. In this case, the question of the formation of protein-nanoparticle conjugates requires a detailed study.

The interactions of lysozyme protein with gold nanoparticles under different conditions of medium acidity were studied by optical methods, the results are shown in Fig.1. It was found that gold nanoparticles can act as stabilizers of lysozyme or interact with it, so that the degree of protein denaturation decreases at alkaline pH values, all other things being equal. And in turn, proteins prevent the aggregation of nanoparticles at acidic pH.

Fig.1. The effect of the acidity on the physical characteristics of colloids HEWL (1016 ml-1), AuNPs (1012 mL-1), HEWL (1016 ml-1) + AuNPs (1012 mL'): (a) size distribution, (b) maximum absorption wavelength, (c) refractive index.

[1] T. Cedervall, I. Lynch, S. Lindman, T. Berggard, E. Thulin, H. Nilsson, K. Dawson, S. Linse. Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles. Proceedings of the National Academy of Sciences, 104(7), 2050-2055, (2007).

[2] S. Shanwar, L. Liang, A. Nechaev, D. Bausheva, I. Balalaeva, V. Vodeneev, I. Roy, A. Zvyagin, E. Guryev. Controlled Formation of a Protein Corona Composed of Denatured BSA on Upconversion Nanoparticles Improves Their Colloidal Stability. Materials, 14, 1657-1673, (2021).

[3] H. Khan, M. Sakharkar, A. Nayak, U. Kishore, A. Khan. Nanoparticles for biomedical applications: An overview. Nanobiomaterials, 357-384, (2018).

[4] P. Gu, X. Liu, Y. Tian, L. Zhang, Y. Huang, S. Su, W. Huang. A novel visible detection strategy for lysozyme based on gold nanoparticles and conjugated polymer brush. Sensors and Actuators B: Chemical, 246, 78-84, (2017).

[5] T. Jing, H. Xia, Q. Guan, W. Lu, Q. Dai, J. Niu, S. Mei. Rapid and selective determination of urinary lysozyme based on magnetic molecularly imprinted polymers extraction followed by chemiluminescence detection. Analytica Chimica Acta, 692(1-2), 73-79, (2011).

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